The Effect of Occlusive and Semi-Occlusive Dressings on the Healing of Acute Full-Thickness Skin Wounds on the Forelimbs of Dogs     

PAUL W. MORGAN DVM  DVSc    ALLEN G. BINNINGTON DVM  MSc  Diplomate ACVS    CRAIG W. MILLER DVM  MVSc  Diplomate ACVS    DALE A. SMITH DVM  DVSc    ANNE VALLIANT BSc  JOHN F. PRESCOTT MA  Vet MB  PhD 《Veterinary surgery : VS》1994,23(6):494-502

This project compared the effects of hydrocolloid (HC) and hydrogel (HG) occlusive dressings and a polyethylene (PE) semi-occlusive dressing on the healing of acute full-thickness skin wounds on the forelimbs of 10 dogs. All treatments resulted in a similar degree of healing at postoperative days 4 and 7. No significant differences existed in the number of wounds that were more than 90% healed at postoperative day 28 between the group treated with the HG dressing and the group treated with the PE dressing. There were significantly fewer wounds more than 90% healed at postoperative day 28 in the group treated with the HC dressing. Wounds under the HG dressing had the largest mean percentage of contraction at postoperative days 21 and 28. Wounds under the HG dressing also had the largest contraction/re-epithelialization ratio (postoperative days 21 and 28) compared with wounds under the PE and HC dressings. Wounds under the PE dressing had a significantly higher mean percentage of re-epithelialization than wounds under both occlusive dressings on postoperative days 14, 21, and 28. Wounds under the two occlusive dressings had exuberant granulation tissue present more often than wounds under the PE dressing. The two occlusive dressings had significantly higher bacterial counts on wounds compared with wounds under the PE dressing; analysis of variance (ANOVA), P = .0008. Wounds under the HC dressing showed the poorest healing in all parameters.  相似文献   

Esterase, malate, and lactate dehydrogenases activity in murine neuroblastoma     

R Prasad  N Prasad  K N Prasad 《Science (New York, N.Y.)》1973,181(98):450-451

Mouse neuroblastoma tumors have only the fifth isozyme band (A(4)) of lactate dehydrogenase, whereas this band is missing in the brain which contained four other bands of lactate dehydrogenase. The alpha-esterase isozyme patterns of tumors, kidney, and brain are similar except that there is an additional slowest-moving form of esterase in all tumor tissues. The malate dehydrogenase pattern is not altered in any of the tissues.  相似文献   

Effect of a Winter Cereal or Legume Crop on the Yield and Nutrient Uptake by Summer Maize/Cowpea Fodder     

M. George  R. Prasad 《Journal of Agronomy and Crop Science》1989,163(3):212-214

A field experiment conducted at the Indian Agricultural Research Institute, New Delhi brought out that summer maize green fodder yield was 31.8 t/ha to 34.1 t/ha, while that of cowpea fodder was only 15.5 to 16 t/ ha. Maize fodder and crude protein yield and NPK uptake was more after a winter legume (lentil) than after a winter cereal (wheat).  相似文献   

SEPTIC COXOFEMORAL ARTHRITIS AND OSTEOMYELITIS IN A DOG     

Lyndell  Levitt  BS  DVM  J. David  Fowler  BS  DVM  MVSc 《Veterinary radiology & ultrasound》1988,29(3):129-132

An 8-year-old male Labrador retriever presented with a rear limb lameness cased by an osteolytic lesion of the left acetabulum and femoral head and neck. Initial bone biopsy study was nondiagnostic. Histopathologic study and culture of the subsequently excised femoral head and neck revealed a Staphyloccocus intermedial arthritis and osteomyelitis. Neoplash was ruled out on the basis of histopathologic analysis.  相似文献   

ANGIOGRAPHIC EVALUATION OF BOVINE INTESTINAL HEALING FOLLOWING INVERTING, EVERTING, AND END-ON ANASTOMOSIS     

A. P. Singh  MVSc  PhD  G. R. Singh  MVSc  A. K. Bhargava  MS  PhD 《Veterinary radiology & ultrasound》1983,24(1):35-40

Angiography during intestinal healing following single-layer inverting, everting, and end-on anastomoses was carried out in 18 calves. Six anastomoses using the same technique were performed in each animal. Six anastomoses, along with one main artery supplying the anastomotic site, were removed from each calf on the first, third, seventh, 15th, 21st, and 28th postoperative days. Angiograms of tubular intestinal segments were obtained after intraarterial infusion of 2–3 ml of sodium iothalamate and lead suspension. Lead-infused intestinal segments were cut open through the mesenteric border to obtain additional angiograms of flat bowel. Angiograms in the early stage of intestinal healing revealed hypervascularity at the cut edges and an avascular zone at the suture line. These changes were more intense with the everting than with the inverting and end-on techniques. The sprouting of vessels at the anastomotic junction and their crossing over started on third postoperative day but were clearly visualized only after the seventh postoperative day. By 15–21 days after operation, anastomotic sites were almost completely revascularized for all techniques. Everted anastomosis elicited more intense vascular response and resulted in earlier vascularization than the other two techniques. No appreciable differences in the pattern of revascularization were observed with inverting and end-on anastomoses. Lead suspension proved to be better than sodium iothalamate for such studies. Angiograms of the open bowel provided more realistic and confirmatory information regarding the sequence of vascular-changes at the suture line.  相似文献   

ARTERIOGRAPHIC ANATOMY OF THE ABDOMINAL AORTA IN THE GOAT, DOG, PIG, AND RABBIT     

Ambika P.  Singh  MVSc  PhD  Gaj Raj  Singh  MVSc  Dwarika N.  Sharma  MVSc  PhD  Janki M.  Nigam  MVSc  PhD  Amar K.  Bhargava  MS  PhD 《Veterinary radiology & ultrasound》1982,23(6):279-281

Arteriographic anatomy of the major branches of the abdominal aorta of rabbits, dogs, pigs, and goats was investigated. The origin and location of the celiac, cranial mesenteric, right renal, left renal, caudal mesenteric, and external iliac arteries were demonstrated, and their placement was described in relation to the iliac bifurcation and lumbar vertebral bodies.  相似文献   

Note: Proline — An inducer of resistance against pearl millet downy mildew disease caused by<Emphasis Type="Italic">Sclerospora graminicola</Emphasis>     

Sathyanarayana?Niranjan?Raj  Nandini?Prasad?Shetty  Hunthrike?Shekar?ShettyEmail author 《Phytoparasitica》2004,32(5):523-527

In an attempt to find a suitable alternative to the otherwise perilous chemical control strategy of disease management, the amino acid proline was evaluated for its efficiency to elicit resistance in pearl millet (Pennisetum glaucum (L.) R. Br.) against downy mildew disease caused bySclerospora graminicola (Sacc.) Schroet both under greenhouse and field conditions. Proline treatment to seeds at 50 mM concentration for 3 h, significantly enhanced the seed germination and seedling vigor of pearl millet in comparison with the control. The same concentration and duration of seed treatment protected the pearl millet plants from downy mildew by offering 58% protection under greenhouse and 67% protection under field conditions. Studies revealed that 3 days were required for proline-treated plants to develop resistance, which was systemic and was sustained throughout the life of the plants. Apart from disease protection, proline was also found effective in enhancing vegetative and reproductive growth of the plants, as evidenced by the increase in height, fresh weight, leaf area, tillering capacity, 1000-seed weight and grain yield in comparison with the control plants. http://www.phytoparasitica.org posting Oct. 3, 2004.  相似文献   

Retinol, β-carotene and β-tocopherol concentrations in mare and foal plasma and in colostrum     

L. S. Gay  D. S. Kronfeld PhD  DSc  MVSc  A. Grimsley-Cook DVM  MS  J. J. Dascanio DVM  A. O. Ordakowski-Burk PhD  R. K. Splan PhD  E. A. Dunnington PhD  D. J. Sklan PhD 《Journal of Equine Veterinary Science》2004,24(3):115-120

Fat-soluble vitamins transfer poorly across the diffuse epitheliochorial placenta of the mare, so the neonatal foal is dependent on its supply via colostrum. Concentrations of retinol (RT), β-carotene (BC), and β-tocopherol (AT) were assayed in samples of plasma, colostrum and milk from nine mares of mixed light breeding and their foals at parturition and days 1, 2, and 4 postpartum. Samples were analyzed simultaneously for RT, BC, and AT using a new, less time-consuming reverse phase high-pressure liquid chromatography (HPLC) method. Feeds were analyzed, and mean daily intakes calculated. Mare plasma RT increased from day 1 to day 4 (P = .033), and plasma AT declined linearly from day 0 to day 4 (P = .006). Colostrum concentrations of all vitamins increased from parturition to day 1, and then declined rapidly over the 4 days. Foal plasma BC increased from parturition to day 4 (P = .080), and plasma AT increased from parturition to day 2 (P < .001), and 4 (P = .060). These observations suggest that three times the current recommendation of vitamin A is sufficient for pregnant mares, in keeping with previous studies of growth, pregnancy, and lactation. In contrast, the linear decrease in mare plasma AT suggests that 1.2 times the current recommendation of vitamin E may be less than optimal for pregnant mares.

Introduction

The diffuse epitheliochorial placenta of the mare does not allow fat-soluble vitamins to cross with ease to the fetus,[1] so their status in the neonatal foal is dependent on colostrum. This first milk contains these vitamins, which are necessary for many functions.Vitamin A is required for vision, reproduction, growth, development, and maintenance of epithelial cells, and osteoclast activity in bone formation. In addition, vitamin A deficiencies reduce resistance to disease, can cause neurological degeneration, and congenital defects.[2] Vitamin E, an antioxidant, prevents lipid peroxidation and cell membrane damage. Its deficiency in horses has been associated with a form of muscular dystrophy and impaired immune function. [3]Studies on the mare and foal have described blood concentrations of one or two of these vitamins,[4, 5 and 6] but only one involved all three. [7] In that study, vitamin concentrations were assayed in mare blood and milk, but only once in foal blood at parturition, so data during the periparturant period is incomplete.The objectives of this enumerative study were to determine concentrations of RT, BC, AT in plasma and colostrum, to evaluate colostral transfer of these vitamins, to assess the adequacy of vitamin intakes of mares, and to develop a time-saving single-run HPLC method.

Materials and methods

Animals

Nine healthy aged multiparous mares of mixed light breeding (13.6 ± 1.5 years, 592 ± 20 kg body mass) and their foals were sampled. The institutional animal care and use committee approved the protocol. Mares were kept on meager late-winter mixed grass pasture, with free access to round bale orchard and fescue grass hay, water, and trace mineral/vitamin salt. They were fed 2 kg of concentrate twice daily at 7:00 and 3:00 . The diet met or exceeded current recommendations for pregnant mares at 11 months gestation for energy.[8] Samples of hay and concentrate were submitted for partial proximate analysis to the Virginia Tech Forage Testing Laboratory where standard AOAC [9] methods are used ( Table 1). Samples of feeds were also taken for analysis of RT, BC, and AT ( Table 2).  相似文献   

Isolation and characterization of neutral lipids of desilked eri silkworm pupae grown on castor and tapioca leaves     

Shanker KS  Shireesha K  Kanjilal S  Kumar SV  Srinivas C  Rao JV  Prasad RB 《Journal of agricultural and food chemistry》2006,54(9):3305-3309

The neutral lipid of desilked eri silkworm pupae (Samia cynthia ricini) grown on two different host plants, castor (Ricinus communis Linn.) and tapioca (Manihot utilizsima Phol.) leaves, was extracted with hexane. The oil content in pupae was estimated to be in the range of 18-20% (dry basis). The pupal oil was found to be enriched with alpha-linolenic acid (ALA) with palmitic acid as the second major fatty acid. The level of ALA in the oil of silkworm pupae was found to be significantly higher (P < 0.001) when grown on tapioca (58.3%) as compared to those grown on castor (42.9%). Other chemical parameters such as percent free fatty acid, peroxide value, phosphorus content, percent unsaponifiable matter, and composition of sterols were also determined in both of the oils and compared. Reversed-phase high-performance liquid chromatography analysis of triacylglycerol molecular species showed that the pupal oil is rich in molecular species with equivalent carbon numbers (ECN) C36, C40, C42, C44, and C48. There was a significantly higher level (P < 0.001) of trilinolenin (C36) in the oil of tapioca-based silkworm as compared to castor-based silkworm pupae. Regiospecific analysis of the oil showed a higher level of ALA at the sn-2 position of silkworm pupae grown on tapioca (60.2%) as compared to those grown on castor (47.3%) oil. Thus, the presence of a large amount of ALA and their predominance at the sn-2 position make the eri pupal oil highly nutritious, provided that the oxidative stability is ensured.  相似文献   

Meiotic analysis of the hybrids between cultivated and synthetic tetraploid groundnuts     

Nalini Mallikarjuna  Sandhya Srikanth  Ravi K. Vellanki  Deepak R. Jadhav  Kumkum Das  Hari D. Upadhyaya 《Plant Breeding》2012,131(1):135-138

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