Alpha-amylase/subtilisin Inhibitor Levels in Australian Barleys     

S.J Jarrett  RJ Marschke  M.H Symons  C.E Gibson  R.J Henry  G.P Fox 《Journal of Cereal Science》1997,25(3):261-266

An enzyme-linked immunosorbent assay (ELISA) was used to determine the bifunctional alpha-amylase/subtilisin inhibitor (BASI) content of barley grain from 11 cultivars grown in six diverse locations in Australia. The inhibitor ranged from 119 to 254 μg/g in 57 barley samples. Genotype had a significant (P<0·05) effect on BASI content but there was no effect due to environment. Total protein varied independently of BASI and was influenced by environment and genotype. BASI content was higher (P<0·05) in malting barley than in feed barley and was correlated positively (r=0·29;P<0·05) with alpha-amylase activity in corresponding malts. The ELISA used monoclonal and polyclonal antibodies raised against purified BASI. In immunoblot analysis the monoclonal antibody showed high specificity for the inhibitor in barley and also detected the inhibitor in wheat. Low levels of inhibitor (mean 3·2 μg/g) were found in 12 Australian wheat cultivars using the ELISA developed for barley. The assay had a linear working range of 5–50 ng/mL with a detection limit of 2 ng/mL. Reproducibility between assays was good (CV=4·9%) but mean recoveries were high, ranging from 116–129% when purified inhibitor was added to barley extracts. The ELISA may have useful applications in brewing research and barley breeding programmes.  相似文献   

The establishment and development of Haemonchus contortus in goats   总被引：1，自引：0，他引：1  

W A Rahman  G H Collins 《Veterinary parasitology》1990,35(3):189-193

Twelve goats were inoculated with 40,000 third-stage Haemonchus contortus larvae and two were killed on each of Days 4, 7, 11, 14, 18 and 21 after inoculation (DAI). The number of worms that established, and the site of development were recorded. More worms established in the fundic, than in the middle or pyloric thirds of the abomasum. Early development occurred within the mucosa; emergence into the lumen started between 7 and 11 days after infection. By 4 DAI, all worms had completed the third moult to the L4 stage. At 11 DAI the majority of the worms were adults. A mean of 13.2% of the female worms had eggs in their uteri at 18 DAI; by 21 DAI more than half of the female worms had eggs in their uteri. The development of H. contortus was essentially similar to that described in sheep.  相似文献   

Total ear canal ablation combining bulla osteotomy and curettage in dogs with chronic otitis externa and media     

S L Beckman  W B Henry  P Cechner 《Journal of the American Veterinary Medical Association》1990,196(1):84-90

Ear canal ablation combining bulla osteotomy and curettage was performed on 44 dogs (n = 72 ears). Indications for the procedure included one or more of the following: chronic nonresponsive otitis externa and/or media (n = 71), tumor in the horizontal portion of the ear canal (n = 1), failed lateral ear resection (n = 11), ossified auricular cartilages secondary to chronic otitis externa (n = 22), failed previous total ear canal ablation (n = 1), and otitis interna (n = 1). In 40 dogs, the surgery was successful in alleviating all clinical signs of otitis externa and media. During the immediate postoperative period, 2 dogs died of causes unrelated to otitis. Complications related to the surgery developed in 9 of the surviving 42 dogs. Ultimately, 95% (40 of the surviving 42) of the dogs were cured by use of this procedure. Surgery successfully resolved the original problems in 97% (66 of 68) of the surgically treated ears of these dogs.  相似文献   

Case of angiosarcoma of the nasal passage of the horse--ultrastructure and differential diagnosis from progressive haematoma     

C W Chan  E A Collins 《Equine veterinary journal》1985,17(3):214-218

An angiosarcoma involving the right frontal and maxillary sinuses in a nine-year-old Thoroughbred mare was studied post mortem by light and electron microscopy. Important diagnostic features included malignant endothelial cells lining ill-defined vascular spaces, supported by other vasoformative elements. Rhabdomyoblasts were also found. The tumour is apparently rarely seen in the nasal passages of the horse.  相似文献   

Effects of stable dust on farm animals-a review     

Mark Collins  Bo Algers 《Veterinary research communications》1986,10(1):415-428

The relationship between air quality-its dust content-and respiratory diseases of animals in the food industry and otherwise in examined. The correlation between dust content and incidence of disease in farm animals is also discussed. Lesions caused either directly or indirectly by dusttransported microorganisms are reviewd. Effects of dust on animal performance are included.This review is an attempt to compile available information on the natural occurrence of stable dust and the possibilities for its measurement and control.  相似文献   

Bovine congenital erythropoietic protoporphyria in a crossbred limousin heifer in Ireland     

Conor G. McAloon  Michael L. Doherty  Henry O’Neill  Michael Badminton  Eoin G. Ryan 《Irish veterinary journal》2015,68(1)

An unusual case of an 11-month-old, black Limousin-cross heifer, with an 8-month history of episodic seizures and photosensitisation, was referred by a veterinary practitioner to the Farm Animal Section of the UCD Veterinary Hospital, School of Veterinary Medicine, University College Dublin, Ireland, in August 2014. Following an investigation, a diagnosis of Bovine Congenital Erythropoietic Protoporphyria (BCEPP) was made. To the authors’ knowledge this is the first report of such a case in Ireland. BCEPP should be considered as a differential diagnosis in young animals displaying periodic seizures and/or photosensitisation.

Electronic supplementary material

The online version of this article (doi:10.1186/s13620-015-0044-3) contains supplementary material, which is available to authorized users.  相似文献   

Effect of the route of administration on the mucosal and systemic immune responses to Lawsonia intracellularis vaccine in pigs          下载免费PDF全文

MG Nogueira  AM Collins  RH Dunlop  D Emery 《Australian veterinary journal》2015,93(4):124-126

In an on‐farm study, 40 weaned piglets aged 3 weeks were vaccinated with Lawsonia intracellularis vaccine orally, IM or IP while a fourth group remained unvaccinated. All vaccinated animals showed increased serum levels of L. intracellularis‐specific IgG antibodies, but significantly elevated concentrations of specific IgG, IgA and cytokines were generated in ileal mucosal secretions from the orally and IP vaccinated pigs when examined at 17 days after vaccination.  相似文献   

Next steps to reduce agricultural tractor overturn fatal and non-fatal injuries     

Cole HP 《Journal of agricultural safety and health》2007,13(4):347-348

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Agroforestry systems recover tree carbon stock faster than natural succession in Eastern Amazon,Brazil     

Cardozo  Ernesto Gómez  Celentano  Danielle  Rousseau  Guillaume Xavier  Silva  Hulda Rocha e  Muchavisoy  Henry Mavisoy  Gehring  Christoph 《Agroforestry Systems》2022,96(5-6):941-956

Agroforestry Systems - The potential of agroforestry systems (AFS) for atmospheric carbon sequestration in degraded tropical lands is of key interest for climate change and rural development...  相似文献   

Conservation agriculture practices have changed habitat use by rodent pests: implications for management of feral house mice     

Ruscoe  Wendy A.  Brown  Peter R.  Henry  Steve  van de Weyer  Nikki  Robinson  Freya  Hinds  Lyn A.  Singleton  Grant R. 《Journal of pest science》2022,95(1):493-503

Journal of Pest Science - The advent of ‘conservation agriculture’ (CA) farming using zero- or no-tillage practices and an accompanying change in crop rotations in the last...  相似文献