ROMANCING IN SCIENCE     

Horn DW 《Science (New York, N.Y.)》1921,53(1359):44-45

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MEDICAL LICENSURE OF NON-MEDICAL DOCTORS     

Horn DW 《Science (New York, N.Y.)》1923,58(1492):79-80

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Virus infections of the European Eel in North Rhine Westphalian rivers     

Linna Danne  Lisa Horn  Anita Feldhaus  Daniel Fey  Sebastian Emde  Heike Schütze  Mikolaj Adamek  John Hellmann 《Journal of fish diseases》2022,45(1):69-76

Viral infections have been suggested to play a role in the decline of the panmictic population of the European eel (Anguilla anguilla). However, despite the importance of knowledge about pathogenic eel viruses, little is known about their spread in the wild European eel population and only a few eel pathogenic viruses have been described so far. In this study, we aimed to investigate the health status of the A. anguilla stock in North Rhine Westphalia (NRW) State of Germany. For this purpose, we examined tissue samples of 16 elvers, 100 yellow eels and 6 silver eels, sampled from the rivers Rhine, Lippe and Ems. Virus detection was performed via a combination of cell culture and PCR. Next to the detection of frequently encountered pathogenic eel viruses (anguillid herpesvirus 1 and eel virus European X (EVEX)), we isolated the eel picornavirus 1 (EPV-1) from tissue of yellow eels and elvers and demonstrate the distribution of EPV-1 in wild eel population in NRW.  相似文献   

Following the Artificially Induced Eel Maturation Process by Means of In Vivo CT Scanning     

Tamás  Müller Róbert  Romvári Miklós  Bercsényi Csaba  Hancz Tamás  Molnár András  Szabó Péter  Horn 《Journal of the World Aquaculture Society》2004,35(2):217-224

Artificially induced maturation of 10 male eels Anguilla anguilla L. weighing 170 ± 30-3 g was followed for 6 wk with in vivo CT scanning in a repeated manner. Since testis tissue has a well defined X-ray density value, the volumetric development could be described. Parallel with this process, a strong decrease of the abdominal fat volume was measured and depicted with the applied surface smoothing method. As an affect of the hCG treatment (250 IU/week per eel) at the third wk, the testis could clearly be recognized on the images. From that time the average testis volume values of the 10 eels were 4,975, 6345, 11,583 and 14,553 mm^J respectively. At the sixth wk, the relative testis volume in the abdomen was 25%. In the fillet a characteristic shift (from -10 to -20 Hounsfield units) of the density histogram towards the fat interval was detected. The average density value of the fillet was found to be zero; this was supported furthermore by the histological analysis of the fillet (fat to muscle rate 0.35-0.40). On the basis of the above findings, the applied CT scanning method was proven to be suitable to follow in vivo the maturation process of male eels.  相似文献   

EXISTENCE OF ONLY ONE VARIETY OF CULTIVATED MANGOSTEEN EXPLAINED BY ASEXUALLY FORMED "SEED"     

Horn CL 《Science (New York, N.Y.)》1940,92(2385):237-238

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Effects of lactational status on forage intake, digestibility, and particulate passage rate of beef cows supplemented with soybean meal, wheat middlings, and corn and soybean meal     

K H Ovenell  K S Lusby  G W Horn  R W McNew 《Journal of animal science》1991,69(6):2617-2623

Sixteen mature, lactating (453 kg) and 16 nonlactating (487 kg) Hereford and Angus x Hereford cows were used to determine effects of different dietary supplements and lactational status on forage intake, digestibility, and particulate passage rate. Supplement treatments and amounts fed (kg/d) were as follows: control, 0; and equal daily amounts of CP from soybean meal (SBM), 1.36; wheat middlings (WM), 3.41; or a blend of corn and soybean meal (corn-SBM; 22% corn and 76% SBM), 3.41. Cows were fed supplements at 0800 and had ad libitum access to prairie hay (4.9% CP) in stalls from 0800 to 1100 and from 1300 to 1600 for three 17-d periods. Lactational status and supplement type did not interact (P greater than .50) for hay DMI, DM digestibility, or particulate passage rate. Cows fed SBM ate more hay DM (P less than .01) and had greater hay DM digestibility (P less than .01) than did cows in other treatment groups. Average hay DMI (kg/100 kg of BW) was 1.95, 2.16, 1.94, and 1.89, and hay DM digestibility was 52, 61, 55, and 53% for control, SBM, WM, and corn-SBM supplements, respectively. Total diet DM digestibility was increased by supplementation (P less than .01), but no differences (P greater than .18) were observed among supplements. Lactating cows ate more (P = .13) hay DM (2.11 vs 1.87 kg/100 kg of BW) and had greater (P less than .05) fecal output (4.6 vs 4.3 kg/d) than did nonlactating cows. Dry matter digestibility and particulate passage rate were not affected (P greater than .35) by lactational status.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

A RETROSPECTIVE ANALYSIS OF 140 DOGS WITH ORAL MELANOMA TREATED WITH EXTERNAL BEAM RADIATION     

David R.  Proulx  DVM  David M.  Ruslander  DVM  Richard K.  Dodge  MS  Marlene L.  Hauck  DVM  PHD  Laurel E.  Williams  DVM  Birgitte  Horn  BVSc  G. Sylvester Price    DVM  PHD  Donald E.  Thrall  DVM  PHD 《Veterinary radiology & ultrasound》2003,44(3):352-359

Despite the early notion that canine oral malignant melanoma is radioresistant, recent data suggest that external beam radiotherapy is effective in local tumor control. However, optimal fractionation schedules have not been established. The high rate of regional and distant metastasis is another problem that has hindered long-term control. The role of chemotherapy in the management of canine oral melanoma has also not been determined. In this study, data from 140 dogs irradiated at North Carolina State University were evaluated with the following objectives: (1) to compare the efficacy of three radiation therapy protocols (36 Gy, 9 Gy x 4 fractions; 30 Gy, 10 Gy x 3 fractions; or >45 Gy, 2-4 Gy x 12-19 fractions) for the treatment of dogs with oral malignant melanoma, (2) to identify any host or tumor factors influencing prognosis, and (3) to determine the impact of systemic chemotherapy on treatment outcome. Information regarding response to therapy, disease progression, and survival were determined from the medical records or from information obtained by telephone or mail survey. Relationships between host, tumor, and treatment variables and outcome measures (response, time to first event, and survival) were evaluated using Fisher's exact test (response) and the Cox regression model (time to first event and survival). The median time to first event for the 140 dogs was 5.0 months (95% C.I., 4-6 months) and the median survival was 7.0 months (95% C.I., 6-9 months). In the univariate analysis, the following variables were associated with increased time to first event and survival: (1) rostral tumor sublocation; (2) lack of bone lysis observed on skull imaging, and (3) microscopic tumor burden. In a multivariate analysis of 111 dogs with complete data for these variables, tumor sublocation, bone lysis, and tumor volume were identified as joint predictors of time to first event (p < .001, p < .001, and p = .04, respectively) and survival (p < .001, p < .001, and p = .05, respectively). There were no differences in response, time to first event and survival between the three radiation therapy protocols used. Systemic chemotherapy had no impact on the development of metastatic disease, time to first event, or survival, although the dosages used in this study were suboptimal. External beam radiation therapy is effective in local disease control of canine oral malignant melanoma; however, the optimal fractionation scheme has yet to be determined. The high metastatic rate observed with this disease and the inefficacy of systemic chemotherapy indicate that further investigation into novel therapies is warranted.  相似文献   

Construction and characterization of a BAC library for sunflower (Helianthus annuus L.)   总被引：1，自引：0，他引：1  

Nehir Özdemir  Renate Horn  Wolfgang Friedt 《Euphytica》2004,138(2):177-183

A bacterial artificial chromosome (BAC) library was constructed using the sunflower (Helianthus annuus L.) restorer line RHA325, which carries the restorer gene Rf1 and the Pl2-gene conferring resistance to downy mildew. High molecular weight DNA was prepared from nuclei using leaf material from two-week old seedlings. The library was constructed using the HindIII site of pBeloBAC11. The current BAC library comprises 104,736 clones. The insert size of the clones varied between 20 and 270 kb, with an average insert size of 60 kb. The whole 1.9× sunflower BAC library was spotted in duplicate on four high-density filters, each carrying 55,296 clones. The content of organellar DNA, which was estimated by colony hybridisation against the mitochondrial probe coxI and the chloroplast probe rbcL, proved to be less than 0.03 and 0.1%, respectively. BAC pools, allowing PCR-based screening, were made and used to identify positive BAC clones for the markers OP-K13_454, closely linked to the restorer gene Rf1. The PCR-based screening was verified by the results obtained for this marker by colony hybridisation.  相似文献   

Inheritance of flower colour and flavonoid pigments in Gerbera     

A. Tyrach  W. Horn 《Plant Breeding》1997,116(4):377-381

The flower colour of Gerbera, an important ornamental cut flower, is derived from carotenoids and flavonoids. The knowledge of enzymological and genetic control of flavonoid biosynthesis is still incomplete. The present paper summarizes the results obtained at our institute between 1981 and 1993. The material for the investigation of phenotypic segregation and segregation of flavonoids after chromatographic analysis came from 408 progenies of controlled crosses. Phenotypic segregation analysis showed acyanic genotypes to be homozygous recessive and recessive epistatic over cyanic genotypes, respectively. This was confirmed by the existence of two loci controlling steps in biosynthesis (fht, dfr or ans) showing recessive mutants and complementary gene action after crosses. Flavone formation is effected by one dominant allele (fns⁺); dominant and recessive genotypes are now available. Regarding anthocyanidin inheritance, an unusual epistasis of 4′-hydroxylation (pelargonidin formation) over 3′,4′-hydroxylation (cyanidin formation) was observed. Final proof of the postulated gene actions will come from enzymological and molecular biological investigations of the chemogenetically defined Gerbera genotypes now available.  相似文献   

Interspecific hybrids of sunflower as a source of Sclerotinia resistance     

S. Rönicke    V. Hahn    R. Horn    I. Grone    L. Brahm    H. Schnabl  W. Friedt 《Plant Breeding》2004,123(2):152-157

Sclerotinia sclerotiorum is considered the most devastating pathogen of sunflower grown in humid environments. In this study, progenies of partial hybrids between Helianthus maximiliani, a wild species that has been shown to be resistant to S. sclerotiorum, and H. annuus were characterized by amplified fragment length polymorphism (AFLP) analysis to identify whether there are introgressions from H. maximiliani into the cultivated sunflower at the molecular level. Wild species‐specific fragments as well as fragments not found in either parent were detected. Progenies tended to cluster together according to the original partial hybrids in the dendrogram by the use of bootstrap procedures. The progenies were studied for their reaction to S. sclerotiorum using artificial inoculation of sunflower heads. Some of the progenies showed a higher level of resistance compared with resistant inbred lines. It was possible to identify two AFLP‐fragments which seem to be linked to Sclerotinia resistance.  相似文献