Developmental Competence of Frozen-thawed Blastocysts from Fair-quality Bovine Embryos Cultured with β-Mercaptoethanol     

T. OTOI  N. KOYAMA  K. YAMAMOTO  N. HORIKITA  S. TACHIKAWA  T. SUZUKI 《Veterinary journal (London, England : 1997)》2000,159(3):392-286

Two-hundred-and-thirty-one fair-quality embryos at the compacted morula stage collected from 89 superovulated cows were cultured in TCM199 or Brinster's BMOC-3 medium with or without 100 microM beta-mercaptoethanol (beta-ME). After 24 h culture, a total of 142 fair-quality embryos developed to the blastocyst stage, of which 106 were subsequently frozen with 1.8 M ethylene glycol. The mean cell number and development rates of frozen-thawed blastocysts from the fair-quality embryos cultured in TCM199 containing beta-ME were higher than those of the fair-quality embryos directly frozen without culture. The pregnancy rates obtained with frozen blastocysts from fair-quality embryos tended to be lower than those of non-cultured fresh fair-quality embryos and cultured fresh blastocysts. These results indicate that the inclusion of beta-ME in pre-freezing culture media improve the development of frozen-thawed blastocysts from fair-quality embryos, but not the pregnancy rate.  相似文献   

A Recessive Lesion Mimic Mutant of Rice with Elevated Resistance to Fungal Pathogens     

Sakae ARASE  Chang-Min ZHAO  Kazuya AKIMITSU  Mikihiro YAMAMOTO  Masahiko ICHII 《Journal of General Plant Pathology》2000,66(2):109-116

A gamma-ray-induced rice mutant, M1009, which spontaneously forms brown specks on leaves was isolated. In appearance, these lesions very much resemble those that are produced during the hypersensitive resistance response of rice to pathogens. A similar phenotype has been associated with a number of mutants in maize or Arabidopsis, which have been briefly categorized as disease lesion mimics. Brown speck formation was suppressed at temperatures of 25°C and above, and was severe at 20°C. Light irradiation is also required to initiate brown specks. In addition, the mutant exhibits heightened resistance to two major fungal pathogens of rice, Magnaporthe grisea and Cochliobolus miyabeanus. Genetic characterization of the mutant has indicated that its les-bs (lesion-brown speck) phenotype segregates as a monogenic recessive mutation. Received 13 September 1999/ Accepted in revised form 15 December 1999  相似文献   

Anaphylaxis after vaccination for cats in Japan     

Megumi YOSHIDA  Keijiro MIZUKAMI  Masaharu HISASUE  Ichiro IMANISHI  Keigo KURATA  Masaki OCHIAI  Masato ITOH  Tadahiro NASUKAWA  Jumpei UCHIYAMA  Hajime TSUJIMOTO  Masahiro SAKAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):149

Severe adverse reactions in cats after vaccination were examined from 316 cases reported to the Ministry of Agriculture, Forestry and Fisheries (MAFF) in Japan during 15-year period from April 2004 to March 2019. We found that 130 (41%) showed anaphylaxis, and 99 (76%) of the 130 cases of anaphylaxis resulted in death. Veterinarians should be well prepared to deal with vaccine-associated anaphylaxis in cats. Bovine serum albumin (BSA) as indicator of purification was detected at high levels in commercially available feline vaccines. BSA might derive from fetal calf serum in culture media. This study provides useful information about anaphylaxis including critical details of the potential clinical signs associated with adverse events to feline vaccination.  相似文献   

Performance of Bangladesh indigenous rice in a weed infested field and separation of allelopathy from resource competition     

Sheikh Muhammad Masum  Mohammad Amzad Hossain  Hikaru Akamine  Jun‐Ichi Sakagami  Takahiro Ishii  Ichiro Nakamura  Md. Asaduzzaman  Prasanta Chitta Bhowmik 《Weed Biology and Management》2019,19(2):39-50

This study aimed to identify the potential allelopathic indigenous rice (Oryza sativa L. ssp. indica) varieties from Bangladesh using a performance study in a weed‐infested field and to assess the extent of allelopathic interference relative to resource competition in a glasshouse experiment. Six varieties – namely, “Boterswar,” “Goria,” “Biron” and “Kartiksail” as the most allelopathic, “Hashikolmi” as weakly allelopathic and “Holoi” as nonallelopathic – were raised following a nonweed control method. The infestation levels of weed species were calculated using Simpson's Diversity Index (SDI), which ranged from 0.2 to 0.56. However, a significant correlation coefficient (0.87, P < 0.001) was obtained from these field data compared with the root inhibition percentage from the laboratory bioassay, and the “Boterswar” variety was the most allelopathic. The interactions between the allelopathic variety “Boterswar,” weakly allelopathic variety “Hashikolmi” and Echinochloa oryzicola via a target (rice)‐adjacent (E. oryzicola) cogrowth culture were determined in a hydroponic arrangement. The relative competitive intensity (RCI) and the relative neighbor effect (RNE) values showed that the crop–weed interaction was facilitation for “Boterswar” and competition for “Hashikolmi” and E. oryzicola in rice/E. oryzicola cogrowth cultures. The allelopathic effects of “Boterswar” were much higher than the resource competition in rice/E. oryzicola cogrowth cultures. The converse was observed for “Hashikolmi.” Moreover, the mineral content of E. oryzicola was severely affected by “Boterswar”/E. oryzicola cogrowth cultures’ exudate solution. Therefore, the allelopathic potential of “Boterswar” variety might be useful for developing the weed‐suppressing capacity of rice, which will likely have a significant influence on paddy weed control.  相似文献   

Oocyte development and serum profiles of vitellogenin and steroid hormone levels in captive female Pacific herring Clupea pallasii during their first maturational cycle     

YASUNORI  KOYA    KIYOSHI  SOYANO  KAZUHISA  YAMAMOTO  HIROYUKI  OBANA  TAKAHIRO  MATSUBARA 《Fisheries Science》2003,69(1):137-145

ABSTRACT:   The present study investigates the relationship between oocyte development and serum steroid hormone levels in captive Pacific herring, Clupea pallasii , during the first reproductive cycle. The process of oocyte development in Pacific herring belongs to the group-synchronous type. Maturity of the ovary was divided into six periods based on histological observation (i.e. immature (April to September), onset of vitellogenesis (August to October), progress of vitellogenesis (October to December), completion of vitellogenesis (December to March), maturation and spawning (March to April) and spent (late April)). The pattern of seasonal change in the gonadosomatic index (GSI) well reflected the ovarian maturity. Serum vitellogenin levels showed good correlation with change in GSI, which increased from September to a peak (4.2 ± 0.3 mg/mL) in March. Serum estradiol-17β (E2) levels elevated from September and reached a peak (15.8 ± 4.2 ng/mL) in December, and remained comparatively high until March, suggesting that the active vitellogenin synthesis during vitellogenesis is controlled by the high E2 level. 17,20β-Dihydroxy-4-pregnen-3-one showed a single sharp peak (2.4 ± 0.28 ng/mL) in early April of the second year, suggesting it was a maturation-inducing steroid in this species.  相似文献   

Comparison of consumption of bluegill by Far Eastern catfish and largemouth bass     

Osamu  KATANO  Tomoyuki  NAKAMURA  Shoichiro  YAMAMOTO 《Fisheries Science》2003,69(5):989-994

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Predominant expression of growth hormone secretagogue receptor in the caudal lobe of chicken pituitary and parallel developmental increase in expression of pituitary GH and proventriculus ghrelin mRNA     

Ichiro YAMAMOTO  Yukinori YOSHIMURA  Akira TSUKADA  Norio KANSAKU  Nobumichi TSUSHIMA  Minoru TANAKA 《Animal Science Journal》2008,79(1):116-121

To examine the involvement of ghrelin in growth hormone (GH) synthesis in the chicken pituitary, the regional distribution of GH secretagogue receptor (GHS‐R)/ghrelin receptor was investigated. Quantitative real‐time polymerase chain reaction (Q‐PCR) analysis revealed that the expression levels of GHS‐R and GH mRNA in the caudal lobe were about fourfold and sevenfold higher in the cephalic lobe of 7 day‐old chickens, respectively. Immunohistochemical analysis showed that GHS‐R immunoreactivity was more abundant in the caudal lobe than in the cephalic lobe, as was the case for GH immunoreactivity. By Q‐PCR, parallel increases were observed in the expression levels of ghrelin mRNA in the proventriculus and GH mRNA in the pituitary from embryonic day 17 to day 7 after hatching, whereas no significant change was found in the expression levels of GHS‐R mRNA in the pituitary during this period. These results suggest that proventriculus‐derived ghrelin may participate in pituitary GH synthesis by acting on its receptor during late embryonic development and the early post‐hatching period in chickens.  相似文献   

Immunohistochemical analysis for G protein in the olfactory organs of soft-shelled turtle, Pelodiscus sinensis     

Shoko NAKAMUTA  Makoto YOKOSUKA  Kazumi TANIGUCHI  Yoshio YAMAMOTO  Nobuaki NAKAMUTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):245-250

In turtles, the epithelia lining the upper and lower chambers of the nasal cavity project axons to the ventral and dorsal parts of the olfactory bulbs, respectively. In a semi-aquatic soft-shelled turtle, Pelodiscus sinensis, more than 1,000 odorant receptor genes have been found, but it is not known where they are expressed. In this study, we aimed to clarify the distribution of cells expressing these genes in the olfactory organs of soft-shelled turtles. Immunoreactions for the Gαolf, the α subunit of G protein coupled to the odorant receptors, were detected on the surface of epithelia lining both the upper and lower chambers of the nasal cavity. The receptor cells in the epithelium of both chambers possessed cilia on the tip of their dendrites, whereas microvillous, non-ciliated, receptor cells were not found. These data suggest that the odorant receptor genes are expressed by the ciliated receptor cells in the upper and lower chamber epithelia. Precise location of the vomeronasal epithelium is not known at present.  相似文献   

Characterization of gene expression in mouse embryos at the 1-cell stage     

Ryoma YAMAMOTO  Ken-ichiro ABE  Yutaka SUZUKI  Masataka G. SUZUKI  Fugaku AOKI 《The Journal of reproduction and development》2016,62(1):87-92

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Improvement and evaluation of the feller-buncher head     

Hiroshi Kobayashi  Masahiro Iwaoka  Takumi Uemura  Ichiro Kageyama 《Journal of Forest Research》1996,1(1):57-59

The high quality forest machines have recently become very popular in Japan. Many improvements on these machines have been made to fit the Japanese forests. This report describes the improvement and evaluation of the feller-buncher head of the prototype machine: FG-35. This head is mounted on the telescopic and knuckle-boom carrier. The prototype machine was improved to cut the trees, felled down on the ground or felled side ways by the storm as in the Kyushu district. And also the machine was improved so that it could be driven well without crashing the chainsaw-bar by the beginners. The testing operation of this machine carried out in Sumita-chou in Iwate and in Kuzu-chou in Oita. The productivity of the machine can be estimated at about 20 m³/h. The results of the improvement on the machine can be said to be good. A portion of this report was presented at the 106th Annual Meeting of the Japanese Forestry Society (1995).  相似文献