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31.
Toru Inoue Satya Parida David J Paton Wilai Linchongsubongkoch David Mackay Yooni Oh Dilok Aunpomma Simon Gubbins Takakiyo Saeki 《Journal of veterinary diagnostic investigation》2006,18(6):545-552
Forty peptides were synthesized corresponding to hydrophilic clusters of amino acids within the sequences of foot-and-mouth disease virus (FMDV) nonstructural proteins (NSP). Six peptides were studied in more detail and the most promising, a 2B peptide, was evaluated in enzyme-linked immunosorbent assay (ELISA) using sera from naive, vaccinated, and vaccinated-and-challenged cattle as well as bovine sera from field outbreaks. The performance of the new NSP peptide ELISA was compared to that of 4 commercial NSP ELISA kits. Antibody to 2B was detectable from the end of the first week to the second week after infection in most of the nonvaccinated animals and by the second to third week in vaccinated-and-challenged animals. The sensitivity of the 2B peptide ELISA was comparable to the 3ABC Ceditest (Ceditest FMDV-NS, Cedi Diagnostics B.V.; Chung et al., 2002). With some modification and further validation, this 2B test could be useful as a screening or conformational NSP test in postvaccination surveillance for FMD. 相似文献
32.
33.
Yasuhiro Inoue Takayuki Matsuura Tatsuji Ohara Koji Azegami 《Journal of General Plant Pathology》2006,72(2):111-118
The entire genome of bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice, and the partial genomes of related phages were sequenced and analyzed. The OP1 genome comprises double-stranded, 4785-bp long DNA with 51.1% G + C content. Fifty-nine open reading frames (ORFs) were detected.
ORF25 had similarity with the tail fiber gene of phages, whose product is related to host specificity. The ORF25 regions were
amplified from four host-range mutants (OP1h, OP1hC, OP1h2, and OP1h2C) by polymerase chain reaction, and their deduced amino acid sequences were compared. Three mutants (OP1hC, OP1h2, OP1h2C) had duplications of a small domain in the N-terminal portion, although there were slight differences in the position of the duplicated sequences. One mutant OP1h had substituted amino acids in the duplication region. New mutants isolated in the laboratory (OP1hC and OP1h2C from OP1 and OP1h2) acquired the ability to lyse strain N5874 belonging to phagovar (lysotype) C. However, they rapidly lost this lytic ability
when incubated with other phagovars. This loss was always accompanied by a loss of the characteristic repeats, suggesting
that the host range of OP1-related phages changed mainly through duplication and deletion of a small domain in ORF25.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AP008979,
AB214312 to AB214316 相似文献
34.
35.
Poxvirus infection in Nile crocodiles (Crocodylus niloticus) 总被引:1,自引:0,他引:1
G S Pandey N Inoue K Ohshima K Okada Y Chihaya Y Fujimoto 《Research in veterinary science》1990,49(2):171-176
An outbreak was encountered of numerous yellowish cutaneous nodules in one- to two-year-old farmed Nile crocodiles (Crocodylus niloticus) in Kasaba Bay Crocodile Farm at Lake Tanganyika in Zambia during 1988. Out of 4000 crocodiles of different age groups, 300 yearlings were affected and 82 of those affected died. The lesions were prominent on the head, especially around the eyelids, the nostrils, both sides of the mouth, ventral neck, ventral pale belly, limbs and the root of the tail. Histologically, the epidermal lesions revealed large focal areas of marked acanthosis accompanied by hyperkeratosis and parakeratosis. In the prickle cells, there were multiple small neutrophilic granular inclusions and large eosinophilic homogeneous cytoplasmic inclusions. Electron microscopically, there were numerous poxvirus particles and matrices in the cytoplasm of many prickle cells. Dark blue homogeneous cytoplasmic inclusions in toluidine blue sections consisted of an electron opaque matrix with mature viral particles (160 x 200 x 230 nm). Furthermore, there were clumps of granular matrix containing immature viral particles (200 x 399 nm) in the cytoplasm. Various features of viral developing processes were observed. 相似文献
36.
Serotypes and antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from diseased dogs 总被引:1,自引:0,他引:1
K Ozaki A Inoue H Atobe E Takahashi S Konishi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):233-239
Strains of Pseudomonas aeruginosa isolated from diseased dogs in Tokyo area during 1983 through 1986 were serotyped and assayed for antimicrobial susceptibility to obtain an epizootiological aspect of canine P. aeruginosa infection. Major sources of specimens were ear and nasal discharges and urine. The results of O-antigen typing using a monoclonal antibody kit showed that the most predominant serotype was type M. Types G and B were also major serotypes. In a yearly distribution of serotypes, type I was almost limited in 1986, and was isolated mainly from surgical wounds, which showed an episode of nosocomial infection, whereas that of type M or B was dispersed during 4 years from 1983 to 1986. As a result of antimicrobial susceptibility test, most canine strains were considered to be susceptible to 4 drugs, which were commonly used in both human and veterinary clinics, in contrast to human isolates. 相似文献
37.
We developed a fast-response multi-chamber system for measuring soil-surface CO2 efflux (Fc). The chambers (90 x 90 x 50 cm, L x W x H) had lids that opened and closed automatically, and were connected in parallel to a single CO2 analyzer equipped with a 16-channel gas sampler. Between measurements the chamber lids were raised to allow precipitation and leaf litter to reach the enclosed soil surface. When a chamber was closed, it was ventilated with well-buffered ambient air (125 l min-1) that entered by an inlet on one chamber sidewall and exited through a large vent on the opposite sidewall. The pressure difference between the inside and outside of the chamber was less than 0.22 Pa. Two additional mixing fans maintained an air speed of 0.3 +/- 0.1 m s-1 at 20 cm above the soil surface. Air was withdrawn continuously from the inlets and outlets of each chamber, and fed sequentially to an infrared CO2 analyzer. With this system, we measured Fc in a 40-year-old temperate Pinus densiflora Sieb. & Zucc. forest from February 8 to May 30, 2001. Mean Fc increased steadily from 0.9 micro mol m-2 s-1 at the beginning of February to 4.6 micro mol m-2 s-1 by the end of May. There was a statistically significant correlation between Fc and surface soil temperature (r = 0.896; P < 0.0001), and the Q10 value was 2.8. Spatial variation of Fc was higher in the non-growing season than in the growing season. Measurements were not interrupted by either rain or snow. 相似文献
38.
Toru Kohama Nobuya Mizoue Satoshi Ito Akio Inoue Kotaro Sakuta Hiroyuki Okada 《Journal of Forest Research》2006,11(4):235-242
We examined the extent to which direct and indirect measures of light and microsite conditions could explain variation in
tree height and diameter at the base of 6-year-old Cryptomeria japonica trees planted in a group selection opening of about 0.32 ha on a steep slope at Shiiba, Miyazaki Prefecture, southern Japan.
We first used the gap light index (GLI) and soil thickness (ST) as directly measured indices. For an indirect measure of light, we used a between-cohort competition index (BCI) estimated from the position and total height of residual trees. For indirect measures of microsite, we examined topographic
indices (slope, plan and profile curvature, average slope gradient, and relative elevation) derived from digital elevation
models (DEMs) with different resolutions ranging from 2 to 10 m. The multiple linear regression using GLI and ST explained about 45% of variation in tree size, while simple regression using only GLI explained about 35%. The contribution of ST was about half of GLI. The multiple regressions using BCI and the topographic indices did not explain any more variation than using BCI alone (R
2 of about 0.26). We conclude that microsite conditions with shallower soil and steeper slope have negative effects on tree
growth in group selection openings, although the relative importance is smaller than light conditions. More comprehensive
studies considering several openings with more heterogeneous topography including different species are needed to generalize
our growth prediction using the indirect measures, which are useful for practical forest management. 相似文献
39.
Endometrial adenocarcinoma in the uterine corpus is a malignant cancer that occurs in menopausal women and aged rodents. Because of the similarities in pathogenesis and morphology of endometrial adenocarcinoma in rodents and humans, prediction of the modes of action (MOA) in uterine carcinogenesis is important for extrapolation of rodent data to humans. Three MOAs have been accepted as major pathways for uterine carcinogenesis in rodents: 1) estrogenic activity, 2) increased serum 17beta-estradiiol (E2) to progesterone (P4) ratio and 3) modulation of estrogen metabolism to produce 4-hydroxyestradiol via P450 induction. Inhibition of estrogen excretion and increased aromatase in situ in the tumor are also a potential pathway. Here, chemicals showing uterine carcinogenicity were chosen from approximately 300 pesticides evaluated in Japan within the past decade, and their mechanisms were predicted using parameters from mechanistic and toxicity studies. Seven pesticides increased uterine tumor formation in rats, and the pathways of 4 pesticides could be predicted based on various mechanistic studies. The MOAs of cyenopyrafen and benthiavalicarb-isopropyl were predicted to be modulation of estrogen metabolism, while those of pyriminobac-methyl and spirodiclofen were predicted to be increased E2 to P4 ratio. The driven pathways of metazosulfuron and isopyrazam could not be predicted using several mechanistic studies. No mechanistic studies have been reported for sedaxane, which has a chemical structure and toxicological profile similar to isopyrazam. Our results indicated that appropriate mechanistic studies are useful for mechanism prediction in risk assessment. From this analysis, a flowchart showing a decision tree for predictive MOAs in uterine carcinogenesis was proposed. 相似文献
40.
Ota Erika do Carmo Cardoso Claudia Andrea Lima Inoue Luis Antonio Kioshi Aoki Zanon Ricardo Basso Silva Tarcila Souza de Castro 《Fish physiology and biochemistry》2022,48(3):735-748
Fish Physiology and Biochemistry - The present study was carried out to evaluate the genotoxic potential of nutritional quality of feed, using erythrocytic nuclear abnormalities assay in Nile... 相似文献