Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism     

Xia Ge  D André d'Avignon  Joseph JH Ackerman  R Douglas Sammons 《Pest management science》2010,66(4):345-348

BACKGROUND: Glyphosate‐resistant (GR) weed species are now found with increasing frequency and threaten the critically important GR weed management system. RESULTS: The reported ³¹P NMR experiments on glyphosate‐sensitive (S) and glyphosate‐resistant (R) horseweed, Conyza canadensis (L.) Cronq., show significantly more accumulation of glyphosate within the R biotype vacuole. CONCLUSIONS: Selective sequestration of glyphosate into the vacuole confers the observed horseweed resistance to glyphosate. This observation represents the first clear evidence for the glyphosate resistance mechanism in C. canadensis. Copyright © 2010 Society of Chemical Industry  相似文献   

High-Tc superconductors in the two-dimensional limit:     

JH Choy  SJ Kwon  GS Park 《Science (New York, N.Y.)》1998,280(5369):1589-1592

The free modulation of interlayer distance in a layered high-transition temperature (high-Tc) superconductor is of crucial importance not only for the study of the superconducting mechanism but also for the practical application of high-Tc superconducting materials. Two-dimensional (2D) superconductors were achieved by intercalating a long-chain organic compound into bismuth-based high-Tc cuprates. Although the intercalation of the organic chain increased the interlayer distance remarkably, to tens of angstroms, the superconducting transition temperature of the intercalate was nearly the same as that of the pristine material, suggesting the 2D nature of the high-Tc superconductivity.  相似文献   

Rupert Paul Herd     

JH Arundel 《Australian veterinary journal》1999,77(12):803-803

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Amoebic meningoencephalitis in a young grey kangaroo (Macropus giganteus)     

JH NORTON  WJ HARTLEY 《Australian veterinary journal》1993,70(11):421-421

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Evaluation of protein-A gold labelling with Dirofilaria immitis antigen coated nitrocelluslose for the detection of anti-heartworm antibody in dogs     

JH TARISH  RB ATWELL 《Australian veterinary journal》1989,66(9):295-296

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Infiltrating Foxp3+ Regulatory T cells and Histopathological Features in Canine Classical and Spermatocytic Seminomas     

JH Kim  SK Chon  KS Im  NH Kim  KW Cho  JH Sur 《Reproduction in domestic animals》2013,48(2):218-222

In humans, regulatory T (T reg) cells are known to play a critical role in both the regulation of immune homoeostasis and the progression of cancer. However, there is little information about the identification, characterization and the function of T reg cells in canine tumours. We identified T reg cells in 28 canine seminoma samples using a Forkhead box P3 (Foxp3) antibody and investigated the relationship between T reg cell infiltration and histopathological features of classical and spermatocytic seminomas (SE and SS, respectively). The Foxp3 protein showed nuclear immunostaining in infiltrating lymphocytes, and Foxp3+ cells were diffused or focally distributed in seminoma tissues. Foxp3+ cells were frequently present in the SS histotype, in seminomas that showed no evidence of tumour cell invasion into the vessels and in seminomas showing a diffuse growth pattern with three cell types. Neither the SE/SS histotype nor the histopathological features of the tumour correlated with Foxp3+ cell counts. These results indicate that Foxp3+ T reg cells may be associated with a less malignant histological phenotype or may not play a critical role in the immune response of canine seminomas. Moreover, Foxp3+ T reg cells may be associated with SS seminoma, but further studies, involving a larger number of samples, are required to better understand whether these cells play a critical role in the immune response in canine seminomas. This is the first report to demonstrate the characteristics of T reg cell infiltration in canine seminoma.  相似文献   

Effect of Dimethyl Sulfoxide on Cell Cycle Synchronization of Goldfish Caudal Fin Derived Fibroblasts Cells     

CH Choresca  OJ Koo  SG Hong  HJ Oh  DK Gomez  JH Kim  BC Lee  SC Park 《Reproduction in domestic animals》2010,45(5):e73-e77

Several studies have previously been conducted regarding cell cycle synchronization in mammalian somatic cells. However, limited work has been performed on the control of cell cycle stages in the somatic cells of fish. The aim of this study was to determine the cell cycle arresting effects of several dimethyl sulfoxide (DMSO) concentrations for different times on different cell cycle stages of goldfish caudal fin‐derived fibroblasts. Results demonstrated that the cycling cells or control group (68.29%) yields significantly higher (p < 0.05) arrest in G0/G1 phase compared with the group treated for 24 h with different concentrations (0.5%, 1.0% or 1.5%) of DMSO (64.88%, 65.70%, 64.22% respectively). The cell cycle synchronization in the treatment of cells with 1.0% DMSO at 48 h (81.14%) was significantly higher than that in the groups treated for 24 h (76.82%) and the control group (77.90%). Observations showed that treatment of DMSO resulted in an increase in the proportion of cells at G0/G1 phase for 48 h of culture. However, high levels of apoptotic cells can be detected after 48 h of culture treated with 1% concentration of DMSO.  相似文献   

Clearing a continent; the eradication of bovine pleuropneumonia from Australia.     

JH Whittem 《Australian veterinary journal》2001,79(5):362-362

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Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato   总被引：6，自引：0，他引：6  

SR Scofield  CM Tobias  JP Rathjen  JH Chang  DT Lavelle  RW Michelmore  BJ Staskawicz 《Science (New York, N.Y.)》1996,274(5295):2063-2065

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two-hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.  相似文献   

基于ALR-GMM的群养猪攻击行为识别算法研究   总被引：2，自引：0，他引：2  

刘冬  何东健  陈晨  STEIBEL Juan  SIEGFORD Janice  NORTON Tomas 《农业机械学报》2021,52(1):201-208

群养猪攻击行为是评估猪群对微环境适应性的重要指标。活动指数模型能够描述猪群行为模式,已经在群养猪攻击行为识别研究中得到初步验证。然而,养殖设施的差异性和动态背景环境等因素所导致的环境适应性差是限制其商业化应用的主要障碍。本文基于递归背景建模思想,在高斯混合模型(GMM)中引入双曲正切函数,提出了一种自适应学习率GMM的活动指数计算方法(ALR-GMM),能够在动态背景环境下准确提取动物活动指数。与经典模型相比,平均相对误差从15.08%降到14.34%。育肥猪攻击行为识别试验中,采用ALR-GMM算法提取行为视频单元的活动指数特征,构建了活动指数最大值、平均值、方差和标准差特征向量,采用线性核函数支持向量机建立分类器。结果表明,本文算法的正确率、灵敏度、特效度和精度分别为97.6%、97.9%、97.7%和97.8%,满足实际应用需求。  相似文献