Genetic diversity and aggressiveness of Calonectria pteridis in Eucalyptus spp.     

R. G. Freitas  R. F. Alfenas  L. M. S. Guimarães  J. L. Badel  A. C. Alfenas 《Plant pathology》2019,68(5):869-877

Calonectria leaf blight, caused by Calonectria pteridis, is currently one of the main foliar diseases in eucalypt plantations in Brazil. In warm and high rainfall regions, the disease can be a limiting factor for eucalypt production when planting susceptible genotypes. The most effective method for controlling this disease in the field is the use of resistant genotypes, which requires knowledge of the genetic variability and aggressiveness of the pathogen population for effective deployment of plant resistance. This work evaluated the genetic diversity and aggressiveness of C. pteridis populations obtained from infected eucalypt plants in Monte Dourado (Pará state) and Imperatriz (Maranhão state), Brazil. To study the genetic diversity, 16 ISSR primers were tested, five of which amplified polymorphic, reproducible and informative bands. Thirty-one closely related genotypes were identified from 84 isolates studied, indicating that the population has a low genetic diversity. The aggressiveness of seven isolates, selected according to geographic origin and their clustering in the ISSR-based dendogram, was determined by inoculation of a hybrid Eucalyptus grandis × E. urophylla clone under controlled conditions. Disease severity was assessed by both measuring the percentage of plant defoliation and assigning a score according to a diagrammatic scale of symptoms. A high correlation between the two evaluation methods was observed, which revealed significant differences in aggressiveness among the isolates. The diagrammatic scale is recommended for disease evaluation because results are obtained much faster, before the occurrence of severe defoliation. No correlation between clustering in the ISSR-based phylogenetic analysis and aggressiveness was observed.  相似文献   

寄主植物中抑制十字花科黑腐病菌Ⅲ型分泌系统小分子化合物的鉴定     

蓝逆寒  苏德山  周乐  覃霞  黄佩芳  杨梅  姜伯乐 《植物病理学报》2019,49(2):262-270

 寻找抑制植物病原菌III型分泌系统的植物源活性小分子化合物,是研发生物安全农药的重要途径之一。本研究采用水煮提取法从十字花科黑腐病菌寄主植物满身红萝卜中提取分离活性小分子化合物,利用高效液相-质谱联用解析出活性物质的单体结构。然后用荧光素酶基因luxAB构建融合报告系统以及定量PCR检测活性物质对十字花科黑腐病菌III型分泌系统的抑制效果,最后采用剪叶接种和压渗接种的方法研究活性小分子物质对十字花科黑腐病菌的生防作用。研究表明,植物鞘氨醇和二氢鞘氨醇对十字花科黑腐病菌III型分泌系统基因的转录表达有一定程度的抑制作用,但是对I、II、IV型分泌系统基因的表达没有明显的抑制作用。植物鞘氨醇在XCM1上影响菌的生长,而二氢鞘氨醇不影响菌的生长。同时,还发现这两种物质能显著降低Xcc在寄主植株满身红萝卜上的病害症状以及能够使Xcc在非寄主植物辣椒上引起过敏反应的能力丧失。该研究结果为深入研究小分子化合物对十字花科黑腐病菌III型分泌系统的作用机制及后续开发植物源抑制剂提供了一定的理论依据。  相似文献   

几种工程措施对黄土区陡峭边坡植被盖度的影响及其机理     

刘乐  孙宏义  张建新  李东泽 《干旱区研究》2019,36(4):1041-1048

陡削以兰州新区的一段陡峭边坡作为试验地点,通过分析在植被重建初期经人工整地后形成的4种微地形(大圆形坑、小圆形坑、条形坑和原状坡面)的植被盖度特征及环境因子(土壤硬度和水分、地表和空气温度、坡位、无纺布)对植被盖度的影响,探寻能有效提高植被盖度的几种工程改造措施。研究表明:①铺设了无纺布坡面的植被盖度显著大于对照坡面;陡峭边坡不同坡位的植被盖度:下坡位>中坡位>上坡位(P<0.05),这与自然坡面植被盖度分布规律一致。②3种微地形的植被盖度均显著大于陡峭坡面,其中条形坑的土壤水分和植被盖度最大,最大盖度维持的时间最长。③微地形和坡面的植被盖度均与土壤水分呈显著正相关,与空气温度、地表温度均呈显著负相关;微地形的土壤硬度(3kg·cm^-2)适宜植物生长,大于该值会对植被生长产生抑制作用;铺设无纺布有利于促进上坡位的植被生长,使坡面植被分布更均匀。研究结果表明,在陡峭边坡坡度、坡位和温度等无法改变的情况下,选择对压实的坡面进行整地翻耕处理,增加长条坑的数量并铺设无纺布等有效的工程改造措施,能显著增加植被盖度,促进植被恢复。  相似文献   

Characterization of the intrarenal renin‐angiotensin system in cats with naturally occurring chronic kidney disease     

Bianca N. Loureno  Amanda E. Coleman  Roy D. Berghaus  Jaime L. Tarigo  Chad W. Schmiedt  Scott A. Brown 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(2):647

  相似文献   

Effect of three diets on the gametogenic development and fatty acid profile of Paracentrotus lividus (Lamarck, 1816) gonads     

Andreia I. G. Raposo  Susana M. F. Ferreira  Rodolfo Ramos  Pedro M. Santos  Catarina Anjos  Teresa Baptista  Carla Tecelo  Jos L. Costa  Ana Pombo 《Aquaculture Research》2019,50(8):2023-2038

In this study, the effects of three diets were investigated to enhance Paracentrotus lividus production for commercial purposes. P. lividus were fed ad libitum for 80 days with: diet A—fresh Codium tomentosum Stackhouse, 1797; diet B—formulated using a jellified mix of macroalgae and vegetables, including C. tomentosum (20%), Coralina sp. Linnaeus, 1758 (17%), cabbage Brassica oleracea var. capitata Linnaeus, 1753 (30%), carrot Daucus carota Linnaeus, 1753 (30%) and agar (3%) as a gelling agent. Diet C consisted of maize Zea mays Linnaeus, 1753 (56%) and New Zealand spinach Tetragonia tetragonoides (Pallas, 1781) Kuntze, 1891 (44%). Their effects on the gonadal and somatic growths, gonadosomatic index (GI) and gametogenesis were evaluated, as well as on the total lipid content and fatty acid composition of sea urchin's gonads. Diet A provided high values of eicosapentaenoic acid (EPA). Gonads of sea urchins fed with diet A were found mostly in growth and maturation stages of gametogenesis and showed the lowest lipid content. Sea urchins fed with diet B presented their gonads in the reabsorption stage and had the highest values of omega‐3 polyunsaturated fatty acids (PUFAs). Sea urchins fed with diet C were in the early stages of gametogenesis and had the highest values of lipid content, plus omega‐6 PUFAs. Once as an ingredient in a balanced mix with vegetables, C. tomentosum can be a key factor to the development of new promising high‐quality and low‐cost feed for P. lividus roe enhancement.  相似文献   

Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms: Evidence for genetic diversity and population structure     

Adolfo Isla  Mnica Saldarriaga‐Crdoba  Derie E. Fuentes  Romina Albornoz  Denise Haussmann  Jorge Mancilla‐Schulz  Alexis Martínez  Jaime Figueroa  Ruben Avendao‐Herrera  Alejandro Yez 《Journal of fish diseases》2019,42(5):721-737

Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic‐resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty‐two Chilean P. salmonisisolates, as well as the type strain LF‐89^T, were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48% of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF‐89^T and EM‐90 strains, as well as a seven‐isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen.  相似文献   

两种杂交石斑鱼子一代杂种优势的微卫星标记分析   总被引：2，自引：3，他引：2  

周翰林  张勇  齐鑫  张海发  张磊  王乐  蒙子宁  刘晓春  林浩然 《水产学报》2012,36(2):161-169

使用6对微卫星引物对两种杂交子一代(青龙斑和虎龙斑)及其亲本(斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼)共5个群体进行微卫星分析,计算等位基因频率、有效等位基因数、基因杂合度、多态信息含量、相似指数和Nei氏遗传距离。结果显示,在5种石斑鱼中,棕点石斑鱼的平均有效等位基因数最大(6.849 3),最小的是鞍带石斑鱼(2.608 6)。6对微卫星引物在这5个群体中具有丰富的多态性,平均多态信息含量(PIC)分别为0.666 7、0.751 3、0.441 9、0.664 0、0.542 6。在平均观测杂合度(Ho)中,虎龙斑和青龙斑的最高均为0.940 0,鞍带石斑鱼的最低为0.508 3。遗传距离和遗传相似率结果显示,青龙斑和虎龙斑均与父本鞍带石斑鱼的亲缘关系较近。杂交子一代群体的等位基因基本来自父母本群体双方,可推断杂交子一代的遗传物质来自父母双方,属两性融合生殖,是真正意义上的杂交种。杂交后代的遗传变异水平明显增强,这是杂种优势得以形成的重要遗传物质基础。  相似文献   

Toxicity of aqueous aluminium to the ectoparasitic monogenean Gyrodactylus salaris     

Arnulf Soleng  Antonio B.S. Polo  Tor A. Bakke 《Aquaculture (Amsterdam, Netherlands)》2005,250(3-4):616-620

Both laboratory and field experiments have demonstrated that aqueous aluminium can act as a paraciticide to the monogenean Gyrodactylus salaris Malmberg, 1957 infecting freshwater salmonids. However, the reproductive conditions of gyrodactylids surviving to cessation of an aluminium exposure is unknown. Therefore, Atlantic salmon (Salmo salar L.) parr infected with G. salaris was experimentally exposed to elevated concentrations of aqueous aluminium for more than 1 month. During this period, the infection increased the first week before it peaked and started to steadily decline approaching elimination. When almost all parasite specimens were eliminated, the water quality was adjusted to normal aluminium-poor freshwater. During the next 3 weeks, the surviving G. salaris were shown to clearly resume their reproduction. The experiment demonstrates the potential of the gyrodactylids to reproduce after cessation of an aluminium exposure. Thus, the as-yet unknown mechanism behind the toxic effects of aluminium on G. salaris survival and reproduction might to some extent be reversible. The present study gives further support for this metal as a parasiticide to G. salaris but demonstrates at the same time that in order to eliminate the parasites totally, the aluminium treatment must eradicate all G. salaris from the skin of the infected hosts. Furthermore, the potential development of resistance to aluminium should also be studied.  相似文献   

我国水产养殖保险发展的几点建议     

王莉  骆乐 《渔业经济研究》2006,(1):32-35

水产养殖业是风险较大的产业,水产养殖者在水产养殖的过程中,难免遭受自然灾害和意外事故,保险机构如能对水产养殖者所遭受的经济损失及时提供经济保障,必将为水产养殖业的稳定发展起到重要作用。本文对中国水产养殖保险发展现状、必要性及存在的问题等进行了阐述,并提出一些建议。  相似文献   

Effect of dietary carbohydrate-to-lipid ratios on growth, lipid deposition and metabolic hepatic enzymes in juvenile Senegalese sole (Solea senegalensis, Kaup)   总被引：3，自引：0，他引：3  

Jorge Dias  Rebeca Rueda-Jasso  Stephan Panserat  Luis E C da Conceição  Emidio F Gomes  & Maria T Dinis 《Aquaculture Research》2004,35(12):1122-1130

A study was undertaken to determine the effect of various dietary carbohydrate‐to‐lipid ratios on growth performance, whole‐body composition and tissue lipid content in Senegalese sole (Solea senegalensis) juveniles. Data on the dietary regulation of key hepatic enzymes of the lipogenic and glycolytic pathways (glucose‐6‐phosphate dehydrogenase, G6PD; malic enzyme, ME; fatty acid synthetase, FAS; pyruvate kinase, PK and glucokinase, GK) were also generated. Four isonitrogenous (crude protein: 52% dry matter (DM)) diets were formulated to contain one of two lipid levels (11% and 21% DM). Within each dietary lipid level, the nature of the carbohydrate fraction (raw or extruded peas) was varied. Triplicate groups of 54 sole (initial body weight: 23.6±1.2 g) were grown in recirculated seawater over 67 days. Fish were fed using automated feeders. At the end of the study, whole‐body, liver, viscera and muscle samples were withdrawn for analyses. During the experimental period, the mean fish weight about doubled in all treatments. No significant differences were found in growth performance (ranging from 1.1% to 1.4% body weight day^?1) among dietary treatments. High‐fat diets increased whole‐body fat content. Similarly, daily fat gain ranged from 0.54 to 0.78 g kg^?1 day^?1 and highest values were found in fish fed high‐lipid diets. Dietary treatments also affected tissue lipid content (liver, viscera and muscle), with highest values in fish fed high‐fat diets. The nature of dietary carbohydrates had little influence on performance criteria, but affected tissue lipid deposition. The activities of G6PD, ME and FAS were depressed by elevated levels of dietary lipid, confirming the inhibitory effect of dietary fats on lipid biosynthesis. At both dietary lipid levels, ME and FAS activities were little affected by dietary carbohydrate. Activities of PK and GK were not affected by the starch level of the diets. In Senegalese sole juveniles, the lipogenic pathway is more susceptible to modulation by dietary means (particularly through lipid intake) than the glycolytic pathway.  相似文献