Effects of food enriched with egg yolk hydrolysate (bone peptide) on bone metabolism in orchidectomized dogs     

Toyokazu KOBAYASHI  Hiroshi KOIE  Arisa WATANABE  Arisa INO  Kazuya WATABE  Mujo KIM  Kiichi KANAYAMA  Kazuya OTSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):503-506

We examined the effects of chicken egg hydrolysate (also known as “bone peptide” or BP) on bone metabolism in 5- to 8-month-old orchidectomized dogs. The bone formation marker serum bone alkaline phosphatase (BAP) and the bone resorption marker urine deoxypyridinoline (DPD) were used as indicators to measure changes in bone metabolism. The following results were observed that Serum BAP was higher in dogs fed BP-enriched food throughout the clinical investigation. Serum BAP was statistically significantly higher in dogs fed BP-enriched food than in dogs fed non-BP-enriched food at 2 months after orchidectomy. This suggests that BP promoted bone formation immediately after orchidectomy.  相似文献   

Real-time PCR genotyping assay for feline erythrocyte pyruvate kinase deficiency and mutant allele frequency in purebred cats in Japan     

Kazuya KUSHIDA  Urs GIGER  Toshihiko TSUTSUI  Megumi INABA  Yoshio KONNO  Kureha HAYASHI  Kana NOGUCHI  Akira YABUKI  Keijiro MIZUKAMI  Moeko KOHYAMA  Yasuyuki ENDO  Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):743-746

Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy caused by mutations of the PKLR gene. A causative mutation of the feline PKLR gene was originally identified in Abyssinian and Somali cats in the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was developed and evaluated for rapid genotyping and large-scale screening for this mutation. Furthermore, a genotyping survey was carried out in a population of four popular purebred cats in Japan to determine the current mutant allele frequency. The assay clearly displayed all genotypes of feline PK deficiency, indicating its suitability for large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele frequency in Abyssinian and Somali cats was high enough to warrant measures to control and prevent the disease. The mutant allele frequency was relatively low in Bengal and American Shorthair cats; however, the testing should still be carried out to prevent the spread of the disease. In addition, PK deficiency should always be considered in the differential diagnosis of anemia in purebred cats in Japan as well as worldwide.  相似文献   

A Study on Borna Disease Virus Infection in Domestic Cats in Japan     

Azusa SOMEYA  Ryoko FUKUSHIMA  Michiko YOSHIDA  Yasuyuki TANAHASHI  Tangmunkhong PRAPEUK  Reiko IIZUKA  Hiroshi HIRAMI  Atsushi MATSUDA  Shunichi TAKAHASHI  Goro KURITA  Takashi KIMURA  Misuzu SEO  Masayuki FUNABA  Yoshii NISHINO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1157-1160

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Primary Ischial Osteosarcoma Occupying the Pelvic Cavity in a Japanese Black Cow     

Eiji NAGAMINE  Kazuya MATSUDA  Chiaki ISHII  Masateru KOIWA  Hiroyuki TANIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(6):891-894

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Comparative Investigation of Body Composition in Male Dogs Using CT and Body Fat Analysis Software     

Toyokazu KOBAYASHI  Hiroshi KOIE  Akiko KUSUMI  Masato KITAGAWA  Kiichi KANAYAMA  Kazuya OTSUJI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):439-446

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Oral Administration of Fermented Probiotics Improves the Condition of Feces in Adult Horses     

Saori ISHIZAKA  Akira MATSUDA  Yosuke AMAGAI  Kumiko OIDA  Hyosun JANG  Yuko UEDA  Masaki TAKAI  Akane TANAKA  Hiroshi MATSUDA 《Journal of Equine Science》2014,25(4):65-72

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Statistical analyses of intra- and interspecific density effects on recruitment of chub mackerel and sardine in Japan     

TATSU KISHIDA  HIROYUKI MATSUDA 《Fisheries Oceanography》1993,2(3-4):278-287

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Experimental induction of secretory and purulent otitis media by the surgical obstruction of the eustachian tube in dogs     

M. TOJO  H. MATSUDA  K. FUKUI  H. SASAI  E. BABA 《The Journal of small animal practice》1985,26(2):81-89

Secretory and purulent otitis media developed after the surgical obstruction of the eustachian tube in dogs. On 3 days after the obstruction, secretory otitis media alone was induced without regard of presence of aerobes in middle ear fluid. On 10 and 15–20 days, two types of otitis media were induced; in the secretory type aerobes were hardly detected, and in the purulent type a considerable number of various species of aerobes were detected. The present results suggest that the eustachian tube obstruction readily generates secretory otitis media and, when aerobes are present in the middle ear, the otitic condition may develop to purulent otitis media.  相似文献   

Drosophila Sex lethal gene initiates female development in germline progenitors   总被引：1，自引：0，他引：1  

Hashiyama K  Hayashi Y  Kobayashi S 《Science (New York, N.Y.)》2011,333(6044):885-888

Sex determination in the Drosophila germ line is regulated by both the sex of the surrounding soma and cell-autonomous cues. How primordial germ cells (PGCs) initiate sexual development via cell-autonomous mechanisms is unclear. Here, we demonstrate that, in Drosophila, the Sex lethal (Sxl) gene acts autonomously in PGCs to induce female development. Sxl is transiently expressed in PGCs during their migration to the gonads; this expression, which was detected only in XX PGCs, is necessary for PGCs to assume a female fate. Ectopic expression of Sxl in XY PGCs was sufficient to induce them to enter oogenesis and produce functional eggs when transplanted into an XX host. Our data provide powerful evidence that Sxl initiates female germline fate during sexual development.  相似文献   

In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans   总被引：5，自引：0，他引：5  

Iwai K  Kishimoto N  Kakino Y  Mochida K  Fujita T 《Journal of agricultural and food chemistry》2004,52(15):4893-4898

Seven kinds of hydroxycinnamic acid derivatives identified as 3-caffeoylquinic acid (3-CQA), 4-caffeolyquinic acid (4-CQA), 5-caffeoylquinic acid (5-CQA), 5-feruloylquinic acid (5-FQA), 3,4-dicaffeoylquinic acid (3,4-diCQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), and 4,5-dicaffoylquinic acid (4,5-diCQA) by MS, 1H NMR, and HPLC analyses were isolated from low-quality (immature) and commercial quality green coffee beans. The quantity of chlorogenic acid isomers (10.4 g/100 g), especially 5-CQA, in commercial green coffee beans [West Indische Bereiding (West India processing beans from Sumatra Island, Indonesia, WIB)] was higher than that in low-quality beans [9.1 g/100 g, Eerste Kwaliteit (Export low-quality beans from Java Island, Indonesia, EK-1, grade 4)], whereas little difference in diCQAs was detected between the two kinds of beans. The free radical scavenging activity of these isolates was evaluated in assay systems using DPPH free radicals and superoxide anion radicals generated by xanthine-XOD. The diCQAs showed strong (1.0-1.8-fold) free radical scavenging activity compared to commonly used antioxidants such as alpha-tocopherol and ascorbic acid. The potency order of superoxide anion radical scavenging activity was diCQAs > caffeic acid, CQAs > 5-FQA. The activities of the diCQAs were twice as effective as those of CQAs and 4 times as effective as that of 5-FQA. The diCQAs also exhibited more potent (2.0-2.2-fold) tyrosinase inhibitory activities compared to CQAs, arbutin, and ascorbic acid. The isolates exhibited antiproliferation activities in four cancer cell lines, U937, KB, MCF7, and WI38-VA. Among these, KB cells were most sensitive (IC50 = 0.10-0.56 mM).  相似文献