Fecal microbiome in dogs with lymphoid and nonlymphoid tumors     

Hyeona Bae  Seul Ki Lim  Hee Eun Jo  Yeonsu Oh  Jinho Park  Hak-Jong Choi  DoHyeon Yu 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):648-659

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Advanced technique for three-dimensional visualization of compound distributions in a rice kernel     

Ogawa Y  Sugiyama J  Kuensting H  Ohtani T  Hagiwara S  Liu X  Kokubo M  Yamamoto A  Kudoh Ki  Higuchi T 《Journal of agricultural and food chemistry》2001,49(2):736-740

A three-dimensional (3D) visualization technique for the compound distribution in a rice kernel was developed. This technique is a combination of sectioning, staining, and digital image postprocessing. By using a special microtome system with adhesive tapes, a set of sequential sections of a rice kernel, which can be preserved with their own set of relative position data, was obtained. A single set of sequential sections was stained by various chemical techniques for the visualization of protein, starch, or lipid content. Each stained section was digitally captured using a CCD imaging device. As the stained areas represent areas containing dye-target complex, the distribution of each compound in the section was visualized in two dimensions. The digitally captured images of a single set of sequential sections were reconstructed to produce a 3D plotting image. As a result, the distributions of various compounds in a rice kernel could be visualized in a new 3D model.  相似文献   

Modeling monthly evaporation using two different neural computing techniques   总被引：2，自引：2，他引：0  

Özgür Kişi 《Irrigation Science》2009,27(5):417-430

Two different artificial neural network (ANN) techniques, multi-layer perceptrons (MLP) and radial basis neural networks (RBNN), are employed in the estimation of monthly pan evaporation. The monthly climatic data, air temperature, solar radiation, wind speed, pressure and humidity, of three stations operated by the U.S. Environmental Protection Agency in California are used as inputs to the ANN models to estimate monthly evaporation. In the first part of the study, the accuracy of MLP and RBNN are compared with each other. The multi-linear regression (MLR) and the Stephens–Stewart (SS) methods are also considered for the comparison. The concern of second part of the study is to investigate the ability of neural computing techniques, MLR and SS models in evaporation estimation using data from nearby station(s). The performances of the models are evaluated using mean square errors, mean absolute relative errors and determination coefficient (R ²). The effect of periodicity on model’s estimation performance is also investigated. Based on the comparisons, it is found that the MLP and RBNN computing techniques could be employed successfully in modeling monthly evaporation process.  相似文献   

X‐ray computed tomography of severed root wounds of Prunus serrulata and Zelkova serrata     

Junhyung Park  Dahye Seo  Ki Woo Kim 《Forest Pathology》2020,50(4)

The performance of laboratory X‐ray computed tomography (XCT) for the non‐destructive imaging of root wood was evaluated. Lateral roots of oriental cherry (Prunus serrulata var. spontanea) and Japanese zelkova (Zelkova serrata) were severed in spring and maintained in soil for 6 months. Without sectioning, XCT revealed the phloem, xylem and vascular cambium structures in the root wood. A virtual transverse section showed a ring of woundwood covering the severed, lateral root of the two trees. Different levels of X‐ray absorption were evident around the cut surfaces of P. serrulata; however, they were rarely detected in Z. serrata. More adventitious roots were observed on Z. serrata than on P. serrulata. Distinct white spots in the rays were only detected in Z. serrata. These results suggest that XCT has potential applications in forest pathology, providing virtual sections of wound closure, wood density distribution, organ redifferentiation, and mineral deposition in root wood.  相似文献   

Discovery and Photoisomerization of New Pyrrolosesquiterpenoids Glaciapyrroles D and E,from Deep-Sea Sediment Streptomyces sp.     

Keebeom Ko  Seong-Hwan Kim  Subin Park  Hwa Seung Han  Jae Kyun Lee  Jin Wook Cha  Sunghoon Hwang  Ki Young Choi  Yoon-Jae Song  Sang-Jip Nam  Jongheon Shin  Seung-Il Nam  Hak Cheol Kwon  Jin-Soo Park  Dong-Chan Oh 《Marine drugs》2022,20(5)

Two new pyrrolosesquiterpenes, glaciapyrroles D (1) and E (2) were discovered along with the previously reported glaciapyrrole A (3) from Streptomyces sp. GGS53 strain isolated from deep-sea sediment. This study elucidated the planar structures of 1 and 2 using nuclear magnetic resonance (NMR), mass spectrometry (MS), ultraviolet (UV), and infrared (IR) spectroscopic data. The absolute configurations of the glaciapyrroles were determined by Mosher’s method, circular dichroism spectroscopy, and X-ray crystallography. Under 366 nm UV irradiation, the glaciapyrroles were systematically converted to the corresponding photoglaciapyrroles (4–6) via photoisomerization, resulting in the diversification of the glaciapyrrole family compounds. The transformation of the glaciapyrrole Z to E isomers occurred in a 1:1 ratio, based on virtual validation of the photoisomerization of these olefinic compounds by ¹H-NMR spectroscopy and liquid chromatography/mass spectrometry (LC/MS) analysis. Finally, when encapsulated in poly(lactic-co-glycolic acid) nanoparticles, glaciapyrrole E and photoglaciapyrrole E displayed significant inhibitory activity against influenza A virus. This is the first report of antiviral effects from glaciapyrrole family compounds, whose biological functions have only been subjected to limited studies so far.  相似文献   

Mixture of Phlorotannin and Fucoidan from Ecklonia cava Prevents the Aβ-Induced Cognitive Decline with Mitochondrial and Cholinergic Activation     

Hye Ju Han  Seon Kyeong Park  Jin Yong Kang  Jong Min Kim  Seul Ki Yoo  Dae-Ok Kim  Gun-Hee Kim  Ho Jin Heo 《Marine drugs》2021,19(8)

The anti-amnesic effect of a mixture (4:6 = phlorotannin:fucoidan from Ecklonia cava, P4F6) was evaluated on amyloid-beta peptide (Aβ)-induced cognitive deficit mice. The cognitive function was examined by Y-maze, passive avoidance, and Morris water maze tests, and the intake of the mixture (P4F6) showed an ameliorating effect on Aβ-induced learning and memory impairment. After the behavioral tests, superoxide dismutase (SOD) activity and thiobarbituric acid-reactive substances (TBARS) contents were confirmed in brain tissue, and in the results, the mixture (P4F6) attenuated Aβ-induced oxidative stress. In addition, mitochondrial activity was evaluated by mitochondrial reactive oxygen species (ROS) content, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) content, and mitochondria-mediated apoptotic signaling pathway, and the mixture (P4F6) enhanced mitochondrial function. Furthermore, the mixture (P4F6) effectively regulated tau hyperphosphorylation by regulating the protein kinase B (Akt) pathway, and promoted brain-derived neurotrophic factor (BDNF) in brain tissue. Moreover, in the cholinergic system, the mixture (P4F6) ameliorated acetylcholine (ACh) content by regulating acetylcholinesterase (AChE) activity and choline acetyltransferase (ChAT) expression in brain tissue. Based on these results, we suggest that this mixture of phlorotannin and fucoidan (P4F6) might be a substance for improving cognitive function by effectively regulating cognition-related molecules.  相似文献   

Use of microsatellite markers to detect quantitative trait loci in Yorkshire pigs   总被引：1，自引：0，他引：1  

Kim CW  Hong YH  Yun SI  Lee SR  Kim YH  Kim MS  Chung KH  Jung WY  Kwon EJ  Hwang SS  Park DH  Cho KK  Lee JG  Kim BW  Kim JW  Kang YS  Yeo JS  Chang KT 《The Journal of reproduction and development》2006,52(2):229-237

To identify genetic markers associated with economic traits in pigs, 157 microsatellite markers were examined in Yorkshire pigs. Thirty eight female Yorkshire pigs were initially examined and six of them were selected as progenitors; half were more than 1.5 standard deviations (SD) above the mean for average daily gain (ADG) and backfat thickness (BFT), and the remaining half were more than 1.5 SD below the mean. These pigs were then mated to male Duroc pigs, and 200 F2 pig offspring were examined for the association of specific alleles with ADG and BFT. To confirm the specific markers identified in the initial analysis, associations of significant markers with economic traits were further examined in 228 additional performance-tested purebred pigs. Twenty-five microsatellite markers were significantly associated with either ADG or BFT, and among these, 17 were associated with both traits. The markers with the highest association to ADG were also associated with BFT. Our study reveals that specific markers could be used to predict economic significance, and confirms several quantitative trait loci (QTL) identified in previous studies. However, further analysis with more closely-spaced microsatellite markers is required to refine predictive values for economic traits and positions of QTL that are reliable for actual phenotypic prediction.  相似文献   

Antioxidant activities of abietane diterpenoids isolated from Torreya nucifera leaves     

Lee WS  Kim JR  Han JM  Jang KC  Sok DE  Jeong TS 《Journal of agricultural and food chemistry》2006,54(15):5369-5374

Investigation on antioxidant compounds from the ethanolic extracts of Torreya nucifera leaves resulted in the isolation of abietane diterpenoids, a known 18-methylesterferruginol (1) and a new 18-dimethoxyferruginol (2). The structures of compounds 1 and 2 were elucidated on the basis of their spectroscopic analyses. Compounds 1 and 2 inhibited the Cu2+-mediated, 2,2'-azobis(2-amidinopropane)hydrochloride-mediated and 3-morpholinosydnonimine-1-mediated low-density lipoprotein (LDL) oxidation in the thiobarbituric acid-reactive substances assay as well as the macrophage-mediated LDL oxidation. Compounds 1 and 2 exhibited the potent antioxidant activities in the conjugated diene production, relative electrophoretic mobility, and apoB-100 fragmentation on copper-mediated LDL oxidation. Compound 1 also suppressed nitric oxide production and inducible nitric oxide synthase expression in lipopolysaccharide-stimulated RAW264.7 cells.  相似文献   

Processes affecting genetic structure and conservation: a case study of wild and cultivated <Emphasis Type="Italic">Brassica rapa</Emphasis>     

Naja Steen Andersen  Gert Poulsen  Bente Anni Andersen  Lars Pødenphant Kiær  Tina D’Hertefeldt  Mike J. Wilkinson  Rikke Bagger Jørgensen 《Genetic Resources and Crop Evolution》2009,56(2):189-200

When planning optimal conservation strategies for wild and cultivated types of a plant species, a number of influencing biological and environmental factors should be considered from the outset. In the present study Brassica rapa was used to illustrate this: to develop Scandinavian conservation strategies for wild and cultivated B. rapa, DNA-marker analysis was performed on 15 cultivated and 17 wild accessions of B. rapa plus 8 accessions of the cross compatible B. napus. The B. rapa cultivars were bred in Sweden and Finland in 1944–1997 and the wild B. rapa material was collected from Denmark, Sweden and United Kingdom. The B. napus accessions were bred within the last 20 years in the Scandinavian countries. Results were based on scoring of 131 polymorphic ISSR markers in the total plant material. A Bayesian Markov chain Monte Carlo (MCMC) approach implemented in NewHybrids demonstrated a clear distinction of B. rapa and B. napus individuals except for three individuals that seemed to be backcrosses. The backcrossed hybrids descended from two Swedish populations, one wild and one escaped. The overall pattern of genetic variation and structure in B. rapa showed that cultivated and wild B. rapa accessions formed two almost separated clusters. Geographical origin and breeding history of cultivars were reflected in these genetic relationships. In addition, wild populations from Denmark and Sweden seemed to be closely related, except for a Swedish population, which seemingly was an escaped cultivar. The study point to that many processes, e.g. spontaneous introgression, naturalisation, breeding and agricultural practise affected the genetic structure of wild and cultivated B. rapa populations.  相似文献   

Comparison of Protective Efficacy between Formalin‐killed and aroA Gene‐knockout Vibrio anguillarum Vaccines in Olive Flounder,Paralichthys olivaceus          下载免费PDF全文

Seung Hyuk Choi  Dong Soo Kim  Ki Hong Kim 《Journal of the World Aquaculture Society》2017,48(6):854-859

The aro genes in bacteria encode enzymes needed for the biosynthesis of aromatic amino acids, and mutant bacteria that are defective in the enzymes can replicate only a limited number in vertebrates owing to the lack or scarceness of chorismate, through which the mutant bacteria of the aro genes become attenuated. In the present study, the 5‐enolpyruvylshikimate‐3‐phosphate synthase (aroA) gene‐knockout Vibrio anguillarum (ΔaroA V. anguillarum) were generated by the allelic exchange method, and its vaccine potential was evaluated in the olive flounder, Paralichthys olivaceus, by comparing the protective efficacy of a formalin‐inactivated V. anguillarum. The LD₅₀ (50% lethal dose) value of ΔaroA V. anguillarum was 1000 times higher than that of wild‐type V. anguillarum in olive flounder fingerlings, and the growth of ΔaroA V. anguillarum was significantly suppressed by coincubation with nonimmune olive flounder serum compared with that of wild‐type V. anguillarum. The survival rates and serum agglutination titers of fish immunized with ΔaroA V. anguillarum were significantly higher than those of fish immunized with the same amount of formalin‐inactivated V. anguillarum, suggesting that although the inactivated V. anguillarum vaccine can provide a high protection in olive flounder, the protective efficacy can be enhanced by immunization with an auxotrophic mutant ΔaroA V. anguillarum.  相似文献