Reduced Germination and Seedling Vigor of Weeds with Root Extracts of Maize and Soybean,and the Mechanism Defined as Allelopathic     

Dhungana  Sanjeev Kumar  Kim  Il-Doo  Adhikari  Bishnu  Kim  Jeong-Ho  Shin  Dong-Hyun 《Journal of Crop Science and Biotechnology》2019,22(1):11-16

Journal of Crop Science and Biotechnology - Due to the negative consequences of synthetic herbicides use and their reducing effectiveness due to development of resistant weeds, promotion of...  相似文献   

Preparation and properties of solid polymer electrolyte based on imidazolium-based ionic liquids for structural capacitors     

Bong-Sang Cho  Jun Choi  Ki-Young Kim 《Fibers and Polymers》2017,18(8):1452-1458

We prepared solid polymer electrolytes (SPEs) composed of poly(ethylene glycol) monomethyl ether acrylate (1A9OMe) and 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIm][OTF]) and 1-ethyl-3-methylimidazolium bis(trifluoromethane sulfonyl) imide ([EMIm][TFSI]) as the ionic liquid. The SPEs formed by appropriately adding ionic liquids in the 1A9OMe prior to thermal cure. The ratio of 1A9OMe and ionic liquid was 1:9, 3:7, and 5:5, respectively. The characterization of solid polymer electrolytes were investigated using Fourier transform infrared spectroscopy in the attenuated total reflectance mode (FTIR-ATR), Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and glavanostatic charge-discharge test. The highest ionic conductivity of SPEs was found to be 4.90×10^?4 S/cm in a 1A9OMe/[EMIm][OTF] of 3:7. As IL contents were increased, the specific capacitance of supercapacitor was increased. The specific capacitance of supercapacitor for ionic liquid with large ion size was lower than that for ionic liquid with smaller ion size.  相似文献   

沉淀法制备的紫杉醇白蛋白微粒包封率测定     

钱佳怡  隋小宇  潘虹  袁橙  董岩  简白羽 《东北林业大学学报》2020,(2):115-117

采用沉淀法制备紫杉醇白蛋白微粒,采用高效液相色谱法(HPLC)测定紫杉醇质量浓度,以二氯甲烷为有机相,采用萃取法分离白蛋白微粒与游离药物,测定其包封率。结果表明:紫杉醇在10~100 mg·L-1范围内线性良好,平均回收率可达到97.6%~101.0%;有机溶剂萃取法测定紫杉醇白蛋白微粒包封率的平均回收率,达94.80%~101.67%。这种方法测定紫杉醇白蛋白微粒回收率准确可靠,适用于沉淀法制备的白蛋白微粒的包封率测定。  相似文献   

马铃薯AP2/ERF转录因子的克隆及植物表达载体构建     

王慧  刘洪  杨奕琦  许思思  熊兴耀  周倩 《分子植物育种》2020,(8):2563-2568

AP2/ERF基因家族转录因子普遍存在于植物中,参与植物体内的各种生物学过程,包括植物的生长发育、生物和非生物胁迫响应等。前期转录组测序的结果发现马铃薯‘加湘1号’一个AP2/ERF家族基因(PGSC0003DMG400012154)在接种晚疫病菌(Phytophthora infestans)24 h后被显著激活。从接种P.infestans 24 h的‘加湘1号’的总RNA中通过RT-PCR获得了该基因CDS序列为885 bp,BLAST分析显示该基因编码一个295个氨基酸残基的蛋白,并且含有1个AP2/ERF结构域,是AP2/ERF转录因子家族中的ERF亚家族的一员。本研究将该基因与XcmⅠ酶切的表达载体pCXSN连接,转化大肠杆菌,通过测序挑选插入正确克隆酶切验证,并成功转化农杆菌。本研究结果为进一步研究该基因的功能提供了帮助。  相似文献   

Milk vetch dwarf virus infection in the Solanaceae and Caricaceae families in Southeast Asia     

Aamir Lal  Eui-Joon Kil  Vo T. B. Thuy  Chairina Fadhila  Phuong T. Ho  Hee-Seong Byun  Hang T. Dao  Ji-Kwang Kim  Sukchan Lee 《Plant pathology》2020,69(6):1026-1033

Milk vetch dwarf virus (MDV) is an important member of the genus Nanovirus and is transmitted by the aphid Aphis craccivora. MDV has multiple single-stranded DNA genome components, each approximately 1 kb, and two or three alpha-satellite molecules. It mainly infects plants of the legume family Fabaceae. Recently, papaya (Carica papaya) collected in Yesan, South Korea, displaying symptoms of leaf yellowing and dwarfism, was identified as a new host for MDV. To examine the geographical distribution of MDV, papaya samples with symptoms were harvested in South Korea, Vietnam, and Taiwan in August 2018, along with tomato and pepper samples grown in adjacent fields in Vietnam. The results revealed the presence of MDV not only in papaya but also in pepper and tomato. This MDV infection in members of the Solanaceae family was confirmed by Southern blot hybridization performed using a PCR product of segment S as a probe. Based on sequence analysis of three MDV components (M, S, and C3), we verified the presence of three different isolates of MDV in these three countries and homology between sequences of isolates from papaya and from members of the Solanaceae from Vietnam. Taken together, our results clearly demonstrate MDV infection in Vietnam and Taiwan for the first time and confirm that MDV can infect economically important pepper and tomato.  相似文献   

川西北地区上二叠统吴家坪组凝灰岩分布地震预测     

徐敏  臧殿光  江娜  徐宝亮  郑虹  龚立  杨晋蓉 《长江大学学报》2021,18(3):21-28

通过对川西北地区的老井进行复查,发现多口井在上二叠统吴家坪组存在大套的凝灰岩,对上述井的地质、测井、地震资料综合分析发现,凝灰岩的岩性以凝灰质泥岩、凝灰质粉砂岩、凝灰质灰岩、沉凝灰岩、凝灰岩、凝灰质白云岩为主,次生溶蚀孔隙发育;凝灰岩在测井曲线和地震剖面上均有明显的响应特征:在测井曲线上表现为明显的低纵波速度、高自然伽马特征,凝灰岩底界在地震剖面上均表现为波峰反射特征.为此,采用模型正演分析技术及波阻抗反演技术,首次对四川盆地川西北地区晚二叠世吴家坪组凝灰岩的分布进行了地震预测:双鱼石构造-剑阁区块的北部地区凝灰岩分布最厚,南部减薄;双鱼石构造东部凝灰岩分布较厚,西部减薄,直至不发育.研究结果表明,晚二叠世吴家坪组在川西北地区沉积了一套凝灰岩,从西北至东南方向减薄;由于凝灰岩物性好,孔隙度高,可成为四川盆地火山岩气藏勘探全新的后备领域,为川西北地区下一步新的勘探领域开发提供了重要研究基础.  相似文献   

养分管理对安溪茶园土壤肥力及茶叶品质的影响   总被引：6，自引：0，他引：6  

刘扬  孙丽莉  廖红 《土壤学报》2020,57(4):917-927

安溪是铁观音的主产区,茶园养分管理滞后已成为该地区茶叶生产的瓶颈,本研究旨在为该地区茶园的养分管理提供科学依据。试验于2015年在安溪县采集了50个茶园的茶青与土壤样品,测定了土壤5项主要肥力指标和茶青中9种主要次生代谢物含量;并根据茶农氮肥用量调研数据,初步将茶园养分管理划分为:少量、中量和过度型等三种方式。限制性主坐标轴分析发现,此分类方式可解释34.4%(P0.001)茶园土壤肥力参数的总体差异,说明此分类方式能反映出不同茶园养分管理的总体水平;并且,养分管理对表层土壤有效磷的影响最为显著。进一步分析表明,养分管理对茶青综合品质的影响可解释品质总差异的7.48%(P0.001);大部分茶青次生代谢物在中量型管理下含量最高。说明养分管理影响安溪茶园土壤的肥力状况,施肥量过高或过低均不利于高品质茶叶的生产,该地区的建议施氮量约为200～400 kg·hm~(–2)·a~(–1)。  相似文献   

Did incentives help municipalities recover from the Great Recession? Evidence from Midwestern cities     

Joshua Drucker  Geon Kim  Rachel Weber 《Growth and change》2019,50(3):894-925

This study assesses the impacts of local business incentives in the largest urban areas of Illinois, Michigan, and Wisconsin, three Midwestern states that share similar histories and settings. We assembled a unique dataset combining information on two types of incentives, tax increment financing districts and tax abatements, together with socio‐economic, geographic, fiscal, and spatial competitive characteristics for all of the municipalities in six metropolitan areas. The outcome measures include employment growth, establishment formation, and business relocation. The analysis extends knowledge of the effects of economic development incentives in two ways. First, we improve upon previous research by incorporating key factors in municipal decisions to offer incentives. Second, we add to limited empirical evidence concerning local incentives following the Great Recession. Variation in the use of incentives reflects not only local decision‐making but also differing fiscal capacities and situations of adaptation to adverse economic conditions, with some governments pulling back on incentives and others initiating new approaches to retain or lure businesses.  相似文献   

贪食迈阿密虫(M.avidus)对4种海水养殖鱼类组织匀浆液的趋化性     

林能锋  陈立志  曾红 《福建畜牧兽医》2021,(2):11-13

通过对贪食迈阿密虫(M. avidus)对4种养殖鱼类组织匀浆液的趋化特性进行研究,以探讨贪食迈阿虫对养殖鱼类的侵染特点。试验结果显示,贪食迈阿密虫对大黄鱼、大菱鲆、黄姑鱼、鲈鱼的眼、脑、脑脊液匀浆液有强烈的趋化效果(P<0.01),对皮肤黏液、鳃、肝、肌肉、肾组织匀浆液的趋化性较弱(P>0.05);对不同鱼类组织匀浆液对贪食迈阿密虫的趋化诱导差异比较结果推测,贪食迈阿密虫对宿主具有一定的选择性。本研究有助于深化贪食迈阿密虫致病性的认识,并为海水养殖鱼类盾纤虫病的预防提供参考资料。  相似文献   

Effect of miRNA-33 on ABCA1/ABCG1 expression in RAW264.7 macrophage-derived foam cells after Hcy treatment     

DAI Pei  GAO Fen  GAO Hong-wei  WANG Yuan  FENG Gao-jie  ZHANG Qin-feng  BAI Rui  QIN Wei-wei  LI Hong  SONG Xiao-su 《园艺学报》2019,35(2):212-217

AIM:To study whether homocysteine (Hcy) inhibits the expression of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) by microRNA-33 (miRNA-33) signaling, and reduces the efficiency of reverse cholesterol transport (RCT).METHODS:RAW264.7 macrophages were induced by oxidized low-density lipoprotein (ox-LDL) to establish foam cell model. Oil red O staining was used to determine whether the model was established successfully. miRNA-33 mimics and miRNA-33 inhibitor were transfected into the cells by Lipofectamine 2000, and the cells were exposed to Hcy at concentration of 5 mmol/L for 24 h. The intracellular lipid droplets were observed by Oil red O staining. The expression of ABCA1 and ABCG1 at mRNA and protein levels was determined by real-time PCR and Western blot. The cellular cholesterol content was analyzed by HPLC, and effluent rate of cholesterol was detected by the method of liquid scintillation counting.RESULTS:Compared with blank control group, the lipid content in miRNA-33 mimics group was increased, and the expression of ABCA1 and ABCG1 at mRNA and protein levels was decreased (P<0.05). The intracellular cholesterol content was increased gradually (P<0.05), and the cellular cholesterol efflux rate was gradually decreased (P<0.05) in miRNA-33 mimics group. Compared with blank control group, the testing results in miRNA-33 inhibitor group were the opposition of those in miRNA-33 mimics group (P<0.05). No diffe-rence of the above indexes among blank control group, miRNA-33 mimics-NC group and miRNA-33 inhibitor-NC group was observed.CONCLUSION:Hcy inhibits the mRNA and protein expression of ABCA1 and ABCG1 through miRNA-33 signaling, and reduces the efficiency of RCT in RAW264.7 macrophage-derived foam cells.  相似文献