Use of cDNA-AFLP for transcript profiling in narrow genetic pools; for example, cucumber (Cucumis sativus L.)   总被引：1，自引：1，他引：1  

K. M. Bae    Y. S. Kwon    I. H. Cho    S. I. Yi 《Plant Breeding》2006,125(5):488-492

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Selection and characterization of a 5-methyltryptophan resistant mutant in Zea mays L.     

Kwon Kyoo Kang  Toshiaki Kameya 《Euphytica》1993,69(1-2):95-101

Summary A maize plant resistant to 5-methyltrytophan (5MT) was selected from M₂ seeds (Zea mays L. Danggin, inbred line) originating from ears treated with ethylmethane sulfonate (0.2%) at 6 hr after self-pollination. Genetic analysis of the progeny of plants selected from a medium containing 50 ppm 5MT showed that 5MT resistance was inherited as a single dominant nuclear gene. This resistance was also expressed in callus and seedling. Analysis of the free amino acids in kernels and calli showed that homozygous resistant plants (MR1) contained higher levels of total free amino acids than sensitive plants and calli. In particular, the their kernels the levels of tryptophan, threonine and serine were, respectively, 4.5, 5.9 and 6.3 times higher than those of the sensitive plants. From the results, it may be expected that mutants resistant to amino acid anologs will be useful not only for studying amino acid biosynthesis but also for improving the nutritional quality of maize.Abbreviations EMS Ethylmethane sulfonate - 5MT 5-methyltryptophan - 2,4-D 2-4-dichlorophenoxyacetic acid  相似文献   

Profiling diversity and comparison of Eastern and Western cultivars of Prunus persica based on phenotypic traits     

Jung Hyun Kwon  Ji Hae Jun  Eun Young Nam  Kyeong Ho Chung  Seong Sig Hong  Ik Koo Yoon  Seok Kyu Yun  Yong Bum Kwack 《Euphytica》2015,206(2):401-415

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Preliminary analysis of recombinant β-tubulin of Pseudocohnilembus persalinus (Ciliophora: Scuticociliatida) as a vaccine antigen candidate against scuticociliatosis     

Sung Mi Kim  Eun Hye Lee  Se Ryun Kwon  Sun Joung Lee  Sung Koo Kim  Yoon Kwon Nam  Ki Hong Kim   《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):21-26

The recombinant β-tubulin of Pseudocohnilembus persalinus was investigated to test whether tubulin could be a suitable antigenic target against scuticociliatosis. The cDNA of P. persalinus β-tubulin has an open reading frame of 1332 bp and had 15 TAA triplets code for glutamine (Q). All 15 TAA/Q triplets of the β-tubulin cDNA were mutated to universal CAA/Q triplets by site-directed mutagenesis and the mutated β-tubulin was expressed in Escherichia coli as a fusion with glutathione S-transferase (GST-BTU). In Western blot analysis, rat sera immunized with recombinant GST-BTU reacted with reduced β-tubulin of P. persalinus. The immunized rat sera showed higher parasiticidal activity than those of control. Ciliates preincubated with heat-inactivated immune sera showed significantly lower proliferation than ciliates preincubated with control sera when exposed to naïve rat sera as a complement source. The present results suggest that the recombinant β-tubulin of scuticociliates may be used as a target antigen for development of vaccine against scuticociliatosis.  相似文献   

Analysis of quantitative trait loci associated with seed germination and coleoptile length under low temperature condition     

Joohyun Lee  Soon Wook Kwon 《Journal of Crop Science and Biotechnology》2015,18(4):273-278

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Differential identification of Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum based on polymorphic regions of glgC and speC genes     

Kang MS  Kwon YK  Jung BY  Kim A  Lee KM  An BK  Song EA  Kwon JH  Chung GS 《Veterinary microbiology》2011,147(1-2):181-185

Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum cause fowl typhoid and pullorum disease in avian species, respectively, and have been of considerable economic importance to the poultry industry in parts of the world. The definitive diagnosis of these diseases can be made only by isolation and identification of the causative agent. However, rapid identification of biovars Gallinarum and Pullorum is not easily feasible due to their common antigenic structure and genomic sequence similarity. We developed a duplex polymerase chain reaction (PCR) assay to identify and discriminate between strains of biovars Gallinarum and Pullorum. Duplex PCR primers were designed to target polymorphic regions of glgC and speC genes showing multiple mutations in the sequenced S. enterica subsp. enterica serovar Gallinarum 287/91 genome and were applied to the specific identification of biovars Gallinarum and Pullorum. Boiled lysates of 131 reference and field strains of Salmonella and other related Gram-negative bacteria were tested to validate the duplex PCR assay. All strains of biovars Gallinarum (n=53) and Pullorum (n=21) tested were correctly identified based on this assay (100% sensitivity) while the other strains (n=57) were PCR negative (100% specificity). These results demonstrate that a highly accurate biovar-specific duplex PCR assay can be performed for the rapid identification and discrimination of biovars Gallinarum and Pullorum from field isolates.  相似文献   

Genetic characterization of H7N2 influenza virus isolated from pigs     

Kwon TY  Lee SS  Kim CY  Shin JY  Sunwoo SY  Lyoo YS 《Veterinary microbiology》2011,153(3-4):393-397

Because pigs have respiratory epitheliums which express both α2-3 and α2-6 linked sialic acid as receptors to influenza A viruses, they are regarded as mixing vessel for the generation of pandemic influenza viruses through genetic reassortment. A H7N2 influenza virus (A/swine/KU/16/2001) was isolated from pig lungs collected from the slaughterhouse. All eight genes of the influenza virus were sequenced and phylogenetic analysis indicated that A/swine/KU/16/2001 originated in Hong Kong and genetic reassortment had occurred between the avian H7N2 and H5N3 influenza viruses. The first isolation of H7 influenza virus in pigs provides the opportunity for genetic reassortment of influenza viruses with pandemic potential and emphasizes the importance of surveillance for atypical swine influenza viruses.  相似文献   

Metabolite profiling of Angelica gigas from different geographical origins using 1H NMR and UPLC-MS analyses     

Kim EJ  Kwon J  Park SH  Park C  Seo YB  Shin HK  Kim HK  Lee KS  Choi SY  Ryu do H  Hwang GS 《Journal of agricultural and food chemistry》2011,59(16):8806-8815

Angelica gigas obtained from different geographical regions was characterized using (1)H nuclear magnetic resonance (NMR) spectroscopy and ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) followed by multivariate data analyses. Principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) score plots from (1)H NMR and UPLC-MS data sets showed a clear distinction among A. gigas from three different regions in Korea. The major metabolites that contributed to the discrimination factor were primary metabolites including acetate, choline, citrate, 1,3-dimethylurate, fumarate, glucose, histamine, lactose, malate, N-acetylglutamate, succinate, and valine and secondary metabolites including decursin, decursinol, nodakenin, marmesin, 7-hydroxy-6-(2R-hydroxy-3-methylbut-3-ethyl)coumarin in A. gigas roots. The results demonstrate that (1)H NMR and UPLC-MS-based metabolic profiling coupled with chemometric analysis can be used to discriminate the geographical origins of various herbal medicines and to identify primary and secondary metabolites responsible for discrimination.  相似文献   

Protective Effects of Platycodon grandiflorum Aqueous Extract on Thioacetamide-induced Fulminant Hepatic Failure in Mice     

Lim JH  Kim TW  Park SJ  Song IB  Kim MS  Kwon HJ  Cho ES  Son HY  Lee SW  Suh JW  Kim JW  Yun HI 《Journal of toxicologic pathology》2011,24(4):223-228

The aim of the present study was to evaluate the protective activity of aqueous extract from Platycodon grandiflorum (BC703) on thioacetamide (TA)-induced hepatotoxicity in mice. We found that BC703 significantly decreased mortality and the change in serum transaminase following TA administration. The group treated with BC703 at doses of 1, 5, and 10 mg/kg produced significant hepatoprotective effects against TA-induced liver damage by decreasing the activities of serum enzymes, nitric oxide and lipid peroxidation in dose-dependent manners. Histopathological studies further substantiated the protective effect of BC703. These results show the hepatoprotective activity of aqueous extract from Platycodon grandiflorum on thioacetamide-induced fulminant hepatic failure.  相似文献   

Development of one-step reverse transcriptase-polymerase chain reaction to detect duck hepatitis virus type 1   总被引：5，自引：0，他引：5  

Kim MC  Kwon YK  Joh SJ  Kwon JH  Kim JH  Kim SJ 《Avian diseases》2007,51(2):540-545

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