寨卡病毒SYBR GreenⅠreal-time PCR方法的建立及应用     

于宁  刘宇梦  李成辉  李卓昕  汪伟  孙文超  鲁会军  金宁一 《中国动物传染病学报》2021,(1)

为建立一种针对寨卡病毒的快速诊断方法,本研究根据寨卡病毒的3’端保守基因序列,设计合成1对引物,建立了检测寨卡病毒的荧光定量PCR方法。结果显示:所建立的检测方法的Ct值与标准品在1.41×10^1~1.41×10^10^ copies/μL具有良好的线性关系,相关性为1,斜率为-3.502;灵敏性结果显示,该方法的检测限度为1.41×10^1 copies/μL,是普通PCR的10000倍;特异性结果显示,对CHIKV、DENV和JEV无特异性扩增,特异性强;重复性试验结果显示,组内和组间变异系数均小于1%,重复性好。本研究建立的SYBR Green I real-time PCR检测方法,可用于寨卡病毒感染的快速诊断。  相似文献   

The M43 domain-containing metalloprotease RcMEP1 in Rhizoctonia cerealis is a pathogenicity factor during the fungus infection to wheat          下载免费PDF全文

PAN Li-jun  LU Lin  LIU Yu-ping  WEN Sheng-xian  ZHANG Zeng-yan 《农业科学学报》2020,19(8):2044-2055

Wheat(Triticum aestivum L.) is an important staple crop for global human. The necrotrophic fungus Rhizoctonia cerealis is the causal pathogen of sharp eyespot, a devastating disease of wheat. Herein, we identified RcMEP1, a zinc metalloproteaseencoding gene from R. cerealis genomic sequences, and characterized its pathogenesis function. RcMEP1 expressed at markedly-high levels during R. cerealis infection process to wheat. The predicted protein RcMEP1 comprises of 287 amino acid residues and contains a signal peptide and a M43 metalloprotease domain harboring the active site motif(HEVGHWLGLYH). The assays of Agrobacterium tumefaciens-mediated transient expression in Nicotiana benthamiana leaves indicated that RcMEP1 is an apoplastic elicitor of cell death, and that the predicted signal peptide functions and is required for secretion and cell death-induction. The purified RcMEP1 protein and its M43 domain peptide were individually able to induce plant cell death and H2 O2 accumulation, and to inhibit expression of host chitinases when infiltrated into wheat and N. benthamiana leaves, while the M43 domain-deleting peptide and negative control lacked the capacity. Moreover, compared with the control pretreatment, the purified RcMEP1 protein or its M43-domain peptide resulted in enhanced pathogenesis in the inoculated wheat, whereas the M43 domain-deleting peptide failed. These results suggest that RcMEP1 acted as an important pathogenicity factor during R. cerealis infection to wheat and that its signal peptide and M43 domain are required for the secretion and pathogenesis of RcMEP1. This study provides insights into pathogenesis role of M43 domain-containing metalloproteases during R. cerealis infection to wheat.  相似文献   

春大棚基质化栽培辣椒品种比较试验     

王书娟  齐合玉  鲁利平  孙超  陈文钊 《蔬菜》2019,(7):76-80

为了提高甜辣椒规模化、产业化生产的竞争力,进一步提高产量、效益和品质,加快甜辣椒栽培管理技术的推广和应用,特引进7个辣椒新品种并采用基质化栽培管理模式进行对比研究。通过对辣椒生长势、品质和产量的比较分析,结果表明:夏驰、国福908、海迈604表现突出,具有个大、肉厚、口感好、品质优、连续坐果性好等特点,而且产量、效益较高,抗病性强,适合设施基质化栽培生产,有很好的推广价值。  相似文献   

利用酵母双杂交系统筛选与草莓镶脉病毒移动蛋白P1互作的寄主因子     

张享享  蒋西子  李帅  蒋磊  江彤 《植物病理学报》2019,49(1):56-63

 构建感染草莓镶脉病毒(SVBV)森林草莓的酵母cDNA文库,利用酵母双杂交系统,筛选出与SVBV P1蛋白互作的15种寄主因子。生物信息学分析发现,这15种寄主因子参与茉莉酸途径、泛素化、光合作用、抗病抗逆、蛋白修饰、蛋白运输和氧化还原等多种生物过程。另外,这些寄主因子还具有其他分子功能,包括氧化还原酶活性、蛋白二硫化物异构酶活性和金属离子结合活性等。本研究初步探讨了P1与寄主因子的互作机理,为揭示SVBV侵染森林草莓以及SVBV在寄主中扩展的分子机制提供理论依据。  相似文献   

Effect of dietary calcium or phosphorus deficiency on bone development and related calcium or phosphorus metabolic utilization parameters of broilers from 22 to 42 days of age          下载免费PDF全文

YANG Yun-feng  XING Guan-zhong  LI su-fen  SHAO Yu-xin  ZHANG Li-yang  LU Lin  LUO Xu-gang  LIAO Xiu-dong 《农业科学学报》2020,19(11):2775-2783

This experiment was conducted to investigate the effect of dietary calcium (Ca) or phosphorus (P) deficiency on bone development and related Ca or P metabolic utilization parameters of broilers from 22 to 42 days of age based on our previous study, which indicated that dietary Ca or P deficiency impaired the bone development by regulating related Ca or P metabolic utilization parameters of broilers from 1 to 21 days of age. A total of 504 one-day-old Arbor Acres male broilers were randomly assigned to 1 of 4 treatments with 7 replicates in a completely randomized design, and fed the normal control and Ca- or P-deficient diets from 1 to 21 days of age. At 22 days of age, the broilers were further fed the normal control diet (0.90% Ca+0.35% non-phytate P (NPP)), the P-deficient diet (0.90% Ca+0.18% NPP), the Ca-deficient diet (0.30% Ca+0.35% NPP) or the Ca and P-deficient diet (0.30% Ca+0.18% NPP), respectively. The results showed that dietary Ca or P deficiency decreased (P<0.05) tibia bone mineral density (BMD), bone breaking strength (BBS), ash content, tibia ash Ca content and serum P content on days 28 and 42, but increased (P<0.05) tibia alkaline phosphatase (ALP) activity of broilers on day 42 compared with the control group. Furthermore, the broilers fed the P-deficient diet had the lowest (P<0.05) tibia BMD, BBS, ash content, serum P content and the highest (P<0.05) serum Ca content on day 28 compared with those fed the Ca-deficient or Ca and P-deficient diets. The results from the present study indicated that the bone development and related Ca or P metabolic utilization parameters of broilers were the most sensitive to dietary P deficiency, followed by dietary Ca deficiency or Ca and P-deficiency; dietary Ca or P deficiency impaired the bone development possibly by regulating serum Ca and P contents as well as tibia Ca content and ALP activity of broilers from 22 to 42 days of age.  相似文献   

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens          下载免费PDF全文

ZENG Xian-ying  CHEN Xiao-han  MA Shu-jie  WU Jiao-jiao  BAO Hong-mei  PAN Shu-xin  LIU Yan-jing  DENG Guo-hua  SHI Jian-zhong  CHEN Pu-cheng  JIANG Yong-ping  LI Yan-bing  HU Jing-lei  LU Tong  MAO Sheng-gang  GUO Xing-fu  LIU Jing-li  TIAN Guo-bin  CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.  相似文献   

Tanshinone ⅡA induces apoptosis via JNK signaling pathway in human osteosarcoma HOS cells     

LU Jian-hong  HUANG Xiao-wen  JIN Yi-jun  YANG Yan-nan  WU Yi-zheng 《园艺学报》2019,35(1):28-33

AIM: To explore the effect of tanshinone ⅡA on human osteosarcoma HOS cells and the underlying mechanism.METHODS: The cell viability and the appropriate dose of tanshinone ⅡA were determined by CCK-8 assay. Colony formation assay and Transwell assay were used to investigate the proliferation and migration abilities of the HOS cells treated with tanshinone ⅡA. The apoptosis of the HOS cells was monitored by Hoechst 33258 staining, transmission electron microscopy and flow cytometry. The protein levels of apoptosis-related molecules and JNK signaling-associated proteins were determined by Western blot. Meanwhile, a JNK inhibitor was added for confirming the relationship between the pathway and apoptosis mentioned above.RESULTS: Tanshinone ⅡA inhibited both HOS cell proliferation and migration in a dose-and time-dependent manner. Exposure of the HOS cells to tanshinone ⅡA resulted in the activation of apoptosis. Tanshinone ⅡA treatment increased the protein levels of cleaved caspase-3, Bax and JNK signaling-associated proteins, and decreased the protein level of Bcl-2, which were reversed by JNK inhibitor SP600125. Moreover, the result of CCK-8 assay revealed that tanshinone ⅡA-induced cell death was alleviated by JNK inhibitor.CONCLUSION: Tanshinone ⅡA induces cell growth inhibition and the activation of apoptosis via JNK signaling pathway in human osteosarcoma HOS cells.  相似文献   

郁金香新品种‘黄玉’     

张艳秋  邢桂梅  张惠华  崔玥晗  鲁娇娇  吴天宇  屈连伟 《园艺学报》2020,47(Z2):3047-3048

‘黄玉’是从郁金香‘Golden Apeldoorn’的芽变中选育的新品种。株高37 cm，花单生于茎顶，鲜黄色，外轮花瓣外侧黄绿色。生育期75 d，盛花期13 d，花朵品质高，抗寒能力强，是优良的盆栽和园林绿化品种。  相似文献   

面向农业监视的流媒体实时传输机制     

林声肯  秦伟博  鲁绍坤 《吉林农业大学学报》2021,43(2):224-230

研究了分布式网络中流媒体的实时传输问题,该问题直接影响农业环境实时监视网络系统的通信效率、实时性和可靠性.考虑到农业地理环境的复杂多样,对流媒体数据传输需要考虑到网络的异构性.通过分析流媒体数据包和音视频帧的依赖关系,建立基于边缘节点的实时传输模型,在模型基础上提出一种基于边缘节点计算的实时传输机制.并通过模拟带宽缓慢变化和不规则抖动两种网络环境在玉米大田中进行仿真和试验分析.对所提出的传输机制的迟延和丢包率进行了深入的分析和认证.结果 表明:这种传输机制能够降低动态网络中流媒体数据的传输迟延和丢包现象,为农业环境监视提供了实时性和可靠性的保证.  相似文献   

The Safety Evaluation of Cefalonium Intramammary Infusion (Dry Cow)     

HUA WeiYi  LIU YiMing  XU Fei  LU YongQiang  KONG Mei  WANG HaiTing  HUANG HuiLi  WANG HongLei  WU LianYong  LI XiuBo 《中国农业科学》2019,52(2):359-366

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