Effects of different concentrations of Chir98014 as an activator of Wnt/beta-catenin signaling pathway on oocyte in-vitro maturation and subsequent embryonic development in Sanjabi ewes     

Sarah Samereh  Hadi Hajarian  Hamed Karamishabankareh  Leila Soltani  Saheb Foroutanifar 《Reproduction in domestic animals》2021,56(7):965-971

The present study was conducted to investigate the effects of the activator factor of the WNT pathway, chir98014, leading to the in vitro sheep oocyte maturation medium, on the cumulus cell development, different nuclear maturation stages and the following process of embryonic development. Experiments included (a) addition of different concentrations (0, 0.1, 0.5, 1 µm) of chir98014 to the maturation medium and evaluation of the cumulus cell expansion, (b) addition of different concentrations of chir98014 to the maturation medium and investigation of different nuclear maturation stages, (c) addition of different concentrations of chir98014 to the maturation medium and examination of the subsequent embryonic maturation process and (d) addition of different concentrations of chir98014 to the embryonic development culture medium (the first 48 hr) and investigation of the subsequent embryonic development process. The extracted data were analysed using the SPSS software, considering the significance level of p < .05 and making the mean comparisons. The results showed that the addition of the 0.1 µM concentration of chir98014 to the maturation medium had no significant effects on the oocyte maturation and embryo development post-fertilization but it enhanced the Cumulus-oocyte complexes (COCs) expansion. In the fourth experiment, the low concentration of chir98014 in the embryo culture media improved the embryo development process, whereas the high one had a detrimental effect on it, as compared to the control group. Thus, the presence of the lower concentrations of this compound in the embryonic culture medium had favourable effects on the development of embryos.  相似文献   

Performance,meat characteristics and economic viability of rabbits fed diets containing banana peel     

Falcone  Diuly Bortoluzzi  Klinger  Ana Carolina Kohlrausch  de Toledo  Geni Salete Pinto  da Silva  Leila Picolli 《Tropical animal health and production》2020,52(2):681-685

Tropical Animal Health and Production - In developing countries, agricultural areas are used to grow ingredients for rabbits’ nutrition instead of food for the human population. In this...  相似文献   

Acute Toxicity and Anesthetic Effects of Clove Oil in Penaeus semisulcatus under Various Water Quality Conditions     

M. Soltani  GH. Marmari  M.R. Mehrabi 《Aquaculture International》2004,12(4-5):457-466

Acute toxicity and anesthetic effects of clove oil were studied in P. semisulcatus (1.8–2.1 g body weight). The EC₅₀ 1-h (the concentration effective for 50% of test animals), LC₅₀ 1-h (the concentration lethal to 50% of test animals after 1 h) and LC₅₀ 24-h (the concentration lethal to 50% of test animals after 24 h) were calculated at concentrations of 25, 130 and 30 mg/l, respectively, at 30°C, salinity 40 ppt, pH 8.6 and dissolved oxygen >6 mg/l. Generally, with increasing concentrations of clove oil, the times required for sedation and anesthesia decreased, while the recovery times increased. At concentrations 50, 100, 150 and 200 mg/l under temperature of 30°C and salinity of 40 ppt, the times required for sedation were 6 ± 0.2, 2.5 ± 0.3, 2 ± 0.08 and 0.5 ± 0.08 min, while times required for complete recovery were calculated to be 4.5 ± 0.3, 5.5 ± 0.17, 6.5 ± 0.25 and 11 ± 0.38 min, respectively. Also, the times required for deep anesthesia were 20 ± 1, 5 ± 0.5, 3 ± 0.4 and 2.2 ± 0.5 min in the above concentrations, while the times required for complete recovery were 10 ± 1, 11 ± 1.5, 14 ± 2.2 and 16 ± 3 min, respectively. Furthermore, considering the times to sedation, deep anesthesia and recovery at different temperatures of 20°C, 25°C, 30°C and 35°C and salinities of 25, 30, 35, 40 and 48 ppt; the combinations of salinity plus temperature and clove oil concentration plus salinity had the greatest and the least effects.  相似文献   

Remediation of metal-contaminated mine tailings by the application of organic and mineral amendments     

Benidire  Leila  Pereira  Sofia  Aboudrar  Wafae  Hafidi  Mohamed  Castro  Paula  Boularbah  Ali 《Journal of Soils and Sediments》2022,22(2):482-495

Journal of Soils and Sediments - Tailings are generally characterized by severe physicochemical conditions that limit the establishment of vegetation. The present study aimed to select suitable...  相似文献   

Effects of plant density and mepiquat chloride application on cotton boll setting in wheat–cotton double cropping system          下载免费PDF全文

CHEN Yuan  LIU Zhen-yu  HENG Li  Leila I. M. TAMBEL  ZHANG Xiang  CHEN Yuan  CHEN De-hua 《农业科学学报》2021,20(9):2372-2381

Sowing cotton directly after harvesting wheat in the Yangtze River Valley of China requires early mature of cotton without yield reduction. Boll-setting period synchronisation and more yield bolls distributed at the upper and middle canopy layers are also required for harvesting. The objective of this study is to quantify the individual and interaction effects of plant density and plant growth regulator mepiquat chloride(MC) on temporal and spatial distributions of yield bolls, as well as yield and yield components. During the 2013–2016 cotton growing seasons, the experiments were conducted on a shortseason cotton cultivar CRRI50 at Yangzhou University, China. Various combinations of plant density(12.0, 13.5 and 15.0 plants m~(–2)) and MC dose(180, 270 and 360 g ha~(–1)) were applied on cotton plants. The combination of 13.5 plants m~(–2) and 270 g ha~(–1) MC resulted in the greatest boll number per unit area, the highest daily boll setting number and more than 90% of bolls positioned within 45–80 cm above the ground. In conclusion, appropriate MC dose in combination of high plant density could synchronize boll-setting period and retain more bolls at the upper and middle canopy layers without yield reduction in the system of direct-seeded cotton after wheat harvest, and thus overcome the labor-intensive problem in current transplanting cropping system.  相似文献   

Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit          下载免费PDF全文

Yuan CHEN  Zhen-yu LIU  Leila I.M. TAMBEL  Xiang ZHANG  Yuan CHEN  De-hua CHEN 《农业科学学报》2021,20(1):100-108

To clarify the effect of the N deficit on the amount of square Bt insecticidal protein, different N application rates(0, 75, 150, 225, and 300 kg ha–1) were imposed on the conventional cultivar Sikang 1(SK-1) and hybrid cultivar Sikang 3(SK-3) during 2015–2016 cotton growth seasons. Under different N application rates, the square number per plant, square volume and square dry weight reduced when the N rates decreased from conventional rate(300 kg ha–1) to 0 kg ha–1. And the square Bt protein content decreased accordingly. The analysis of N metabolism showed that soluble protein content, GPT and GOT activities decreased, free amino acid, peptidase and protease activities increased under N deficit. Correlation analysis indicated that the reduced Bt protein content under N deficit was related to altered N metabolism. In conclusion, square development and the amount of square Bt toxin both decreased under N deficit, indicating that promoting the square development under appropriate N application rate would also promote the insect resistance during squaring stage.  相似文献   

INFLUENCE OF VARIOUS PHYSICAL PARAMETERS ON ANTHER CULTURE OF BARLEY     

Ferdaous Guasmi  Walid Elfalleh  Hédia Hannachi  Khadija Fères  Leila Touil  Nidhal Marzougui 《Journal of plant nutrition》2013,36(5):836-847

This study describes the effect of some parameters on anther culture of barley to optimize the plant regeneration condition of this species. The embryo formation and plant regeneration from anthers of three barley cultivars (‘Igri’, ‘Saida’ and ‘Libya’) were investigated. The effects of length of flag leaves, stage of microspore development, and pre-treatments (mannitol or cold pre-treatment) of anthers were investigated. Results showed significant responses. Anthers at the mid uninucleate to mid-late uninucleate stage gave the best anther culture response showing 80, 60, and 30% anther development in ‘Igri’, ‘Saida’ and ‘Libya’ barley cultivars, respectively. The use of mannitol (0.3 M) or 20 days cold pre-treatments showed the best results for embryo and green-plant production.  相似文献   

Shoot growth,plant tissue elemental composition,and soil salinity following irrigation of alfalfa and tall fescue with high‐sulfate waters 1     

Jennifer W. MacAdam  Daniel T. Drost  Lynn M. Dudley  Nader Soltani 《Journal of plant nutrition》2013,36(9):1137-1153

Groundwater contaminated with sulfate (SO₄ ^2‐) at concentrations higher than allowable for drinking water might still be usable for irrigation. Objectives were to determine the growth response and mineral uptake of two forage crops irrigated with waters containing SO₄ ^2‐ at concentrations ranging from 175 to 1743 mg/L, and with electrical conductivities (EC) ranging from 1.2 to 3.6 dS/m. Plants were grown for 12 weeks in 8‐L pots containing a calcareous sandy loam and were harvested at 4, 8, or 12 weeks for plant growth measurements and tissue analysis. Digested leaves, stems, and reproductive tissues were analyzed by inductively coupled plasma (ICP) spectroscopy at each harvest, as were saturated soil paste extracts. Shoot growth of tall fescue (Festuca arundinacea Schreb.) was not affected by irrigation water treatment, whereas shoot growth of alfalfa (Medicago sativa L.) was increased by a moderate level of soil solution SO₄ ^2‐ Sulfur (S), boron (B), magnesium (Mg), sodium (Na), and zinc (Zn) concentrations in shoot tissues of both species showed a tendency to increase with increasing SO₄ ^2‐ content of irrigation water. Shoot tissue concentration of molybdenum (Mo) increased with maturation in both species, while the concentrations of B, potassium (K), manganese (Mn), Na, and Zn decreased. Soil saturated paste extract concentrations of Mg and Na increased with irrigation water Mg and Na concentrations, while Ca and S concentrations in the soil solution became saturated at the higher irrigation water concentrations of these elements.  相似文献   

Effects of gibberellic acid on ovarian biochemical composition and ecdysteroid amounts in the migratory locust Locusta migratoria (Orthoptera,Acrididae)     

Khemais Abdellaoui  Monia Ben Halima-Kamel  Acheuk Fatma  Noureddine Soltani  Nadia Aribi  Mohamed Habib Ben Hamouda 《国际虫害防治杂志》2013,59(1):68-72

Migratory locusts represent the most important crop pests in Africa and Asia. Because environmental damages are associated with the use of synthetic insecticides in locust and grasshopper control, new environmentally acceptable approaches are becoming increasingly important. The effects of gibberellic acid (GA₃), a plant growth regulator, was investigated by topical application and ingestion on the reproductive physiology and ecdysteroids production of the migratory locust Locusta migratoria at concentrations of 125, 625, 3125, 4125, 5125 and 6125 μg/mL. The treatment applied to adult females during the pre-ovipositional phase provokes a significant adverse influence on their reproductive potential. Indeed, GA₃ significantly reduced both fecundity and fertility. Gibberellic acid also caused disturbances in the incorporation of the hemolymph metabolites (proteins, carbohydrates and lipids) in the oocyte resulting in a significant reduction in their concentrations in the ovaries. Ecdysteroid production was measured by an enzymo-immunoassay. GA₃, administered by ingestion to the adult females, significantly reduced the ecdysteroid titers in ovaries and freshly laid eggs.  相似文献   

Demographics of patients with surgical and nonsurgical cystic echinococcosis in East Azerbaijan from 2001 to 2012     

Vahedi MA  Vahedi ML 《Pakistan journal of biological sciences: PJBS》2012,15(4):186-191

Echinococcosis is very frequent helminthic infection in human being. The disease is endemic in Iran and particularly in its northwestern region, East Azerbaijan. The objective of this study was to study demographics of patients with cystic echinococcosis in East Azerbaijan; as well as to repot the sites of involvements. In this cross-sectional study, 318 patients with surgical and nonsurgical cystic echinococcosis were recruited from three referral centers in Tabriz within a 10-year period of time. The patients' demographics (age and gender) and the site of infection were investigated. Among the patients, females were predominant (57.5 vs. 42.5%). The mean age of patients was 32.59 +/- 18.47 (range: < 1-87) years and the most frequently age group was 20-30 years (23.6%), followed by the age groups of 30-40 years (21.7%), 10-20 years (14.2%), 40-50 years (11.9%), 50-60 years (11.3%), < 1 year (6%), 60-70 years (4.7%), 1-10 years (4.1%) and finally, > 70 years (2.5%). The lung was the most frequently infected organ (48.1%), followed by the liver (28.9%), gallbladder (3.5%), spleen and kidney (each one in 2.2%), abdomen, intestine, pleura, bronchus and mediastinum (each one in 1.6%), chest wall and heart (each one in 1.3%), brain, pericardium, facial sinus, pancreas and esophagus (each one in 0.6%) and the ovary, uterus, axillary region, pelvis and femur (each one in 0.3%). In conclusion, this study showed that the patients with hydatid cysts in East Azerbaijan Province are mainly females in the age group of 20-40 years. The lung and liver are the major sites of infection in these patients.  相似文献