Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method     

Noboru SASAKI  Thandar OO  Yoshikazu YASUDA  Takahiro ICHISE  Noriyuki NAGATA  Nozomu YOKOYAMA  Kazuyoshi SASAOKA  Keitaro MORISHITA  Shouta MM NAKAYAMA  Mayumi ISHIZUKA  Kensuke NAKAMURA  Mitsuyoshi TAKIGUCHI  Yoshinori IKENAKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1634

We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2–16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.  相似文献   

First record of infection by Trypanosoma sp. of Colossoma macropomum (Serrasalmidae), a neotropical fish cultivated in the Brazilian Amazon     

Roselene Nogueira Rodrigues  Marcos Sidney Brito Oliveira  Marcos Tavares-Dias 《Journal Of Applied Aquaculture》2018,30(1):29-38

Fish farming has grown rapidly in Brazil over the last two decades, due in part to the availability of water and species with potential for cultivation, such as Colossoma macropomum. This farmed fish, however, can be affected by parasitic infections with high mortality rates (>35%). The main objective of the present study was to describe the first occurrence of the Trypanosoma species in Colossoma macropomum, which occurred in fish cultured in the Brazilian Amazon region, as well as to morphologically characterize the trypomastigotes and the impact of these hemoparasites on the body condition of the hosts. The trypanosomes found in the blood of Colossoma macropomum were morphologically characterized by the size of the trypomastigote form, indicating the presence of only one morphotype. Of the 39 hosts examined from one fish farming, 41.0% were infected by Trypanosoma sp., with low levels of infection (mean blood density of 3.7/μL). The condition factor of the asymptomatic hosts was not affected by hemoparasitism. There was no correlation between the abundance of Trypanosoma sp. and the condition factor and size of the hosts. Finally, our understanding of host-parasite interactions and the detection of emerging diseases are fundamental for aquaculture.  相似文献   

Chemical Characterization and Release of Polyphenols from Pecan Nut Shell [<Emphasis Type="Italic">Carya illinoinensis</Emphasis> (Wangenh) C. Koch] in Zein Microparticles for Bioactive Applications     

Itamara Kureck  Priscila de Brito Policarpi  Isabela Maia Toaldo  Matheus Vinícius de Oliveira Brisola Maciel  Marilde T. Bordignon-Luiz  Pedro Luiz Manique Barreto  Jane Mara Block 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(2):137-145

The pecan nut [Carya illinoinensis (Wangenh) C. Koch] is a natural source of polyphenols with antioxidant properties. In this study, the encapsulation of aqueous and hydroalcoholic extracts of pecan nut shell were evaluated for the release of bioactive compounds and antioxidant potential in order to explore food applications using zein as encapsulating agent. The extracts showed high contents of total phenolics, condensed tannins and high antioxidant activity. Concentrations of proanthocyanidins were 9-fold higher in hydroalcoholic extracts. The LC-DAD analysis showed that catechins were the major phenolic compounds in samples, with epigallocatechin levels up to 138.62 mg mL^?1. Zein microparticles loaded with aqueous extract released 2.3 times more phenolic compounds than the hydroalcoholic extracts and the DSC thermograms showed that extracts of pecan nut shell remained thermally stable up to 240 °C. The zein microcapsules obtained in this study were efficiently encapsulated and represent an interesting additive due its high antioxidant capacity, physicochemical characteristics and morphology. The use of zein microparticles combined with natural extracts constitute a step forward in the improvement of current technology for delivering phenolic compounds with applications in functional foods and nutraceuticals.  相似文献   

Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation     

Hilton Marcelo de Lima Souza  Lara Durães Sette  Adolfo José da Mota  Joaquim Ferreira do Nascimento Neto  André Rodrigues  Tássio Brito de Oliveira  Fernando Mendes de Oliveira  Luiz Antônio de Oliveira  Hileia dos Santos Barroso  Sandra Patricia Zanotto 《Water, air, and soil pollution》2016,227(12):431

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A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture   总被引：1，自引：0，他引：1  

X. Serrat  M. Cardona  J. Gil  A. M. Brito  L. Moysset  S. Nogués  E. Lalanne 《Euphytica》2014,195(1):31-44

Certified seed producers systematically select and propagate registered varieties year after year in order to maintain their uniformity and the original registered cultivar traits. However, natural mutations, spontaneous breeding between varieties and alien grain contamination can introduce undesirable variability. NRVC 980385 is a temperate japonica rice cultivar (Oryza sativa ssp. japonica) first registered in Spain in 2002. In 2005 certification tests detected a plot differing from the original traits in terms of uniformity and height suggesting the presence of a certain heterozygosis. This material was therefore seen as an opportunity to obtain newly stabilized doubled haploid (DH) lines which could compete in the Spanish short grain seed market. In this study, an in vitro anther culture protocol is defined which also covers the field tests selection to obtain four new, improved and stabilized DH derived lines ready to be registered for commercial proposes. This took just 4 years from the initial anther collection until new lines were grown in large scale field trials. Consequently, this protocol reduces the time for obtaining field assessed DH lines thereby having considerable advantages over other techniques by both maintaining the original registered cultivars and/or generating new derived varieties.  相似文献   

Biogeography and conservation of taxa from remote regions: An application of ecological-niche based models and GIS to North-African canids     

José C. Brito  André L. Acosta 《Biological conservation》2009,142(12):3020-3029

In North Africa, and especially in the Sahara Desert, biodiversity is poorly known. Of the five widespread canid species present, one is Data Deficient, three are considered widespread although habitat selection could limit their area of occupancy, and distribution maps available are coarse for conservation planning. This study identifies biogeographic patterns in North-African canids through the combination of high resolution presence data with 16 environmental factors. Predictive models trained in north-west Africa are projected to all North Africa. canids exhibited distinct biogeographical affinities. GIS tools and Maximum Entropy models identify a mixture of climatic and habitat factors as main predictors of species occurrence. Suitable habitats for North-African canids are mostly fragmented: probable occurrence was identified for Canis aureus in Saharan peripheral regions and mountains, for Vulpes pallida in a narrow band along the Sahel and in southern Saharan mountains, for Vulpes rueppellii throughout the Sahara, for Vulpes vulpes in northern Africa until the Sahara northern limit, and for Vulpes zerda in almost all Sahara. Areas of potential sympatry between species with similar niches and parapatric ranges are identified along relatively narrow bands. The small pixel size of projections allows the identification of suitable refuges for species otherwise absent in the driest Saharan habitats, providing framework data for the definition of the global conservation status of V. pallida, and conservation strategies for the guild. The biological value of Saharan mountains is emphasised as they constitute isolated suitable areas. Ecological-niche based models should be developed for other endangered Saharan vertebrates.  相似文献   

Modeling genetic differences of combined broiler chicken populations in single-step GBLUP     

Matias Bermann  Daniela Lourenco  Vivian Breen  Rachel Hawken  Fernando Brito Lopes  Ignacy Misztal 《Journal of animal science》2021,99(4)

The introduction of animals from a different environment or population is a common practice in commercial livestock populations. In this study, we modeled the inclusion of a group of external birds into a local broiler chicken population for the purpose of genomic evaluations. The pedigree was composed of 242,413 birds and genotypes were available for 107,216 birds. A five-trait model that included one growth, two yield, and two efficiency traits was used for the analyses. The strategies to model the introduction of external birds were to include a fixed effect representing the origin of parents and to use unknown parent groups (UPG) or metafounders (MF). Genomic estimated breeding values (GEBV) were obtained with single-step GBLUP using the Algorithm for Proven and Young. Bias, dispersion, and accuracy of GEBV for the validation birds, that is, from the most recent generation, were computed. The bias and dispersion were estimated with the linear regression (LR) method,whereas accuracy was estimated by the LR method and predictive ability. When fixed UPG were fit without estimated inbreeding, the model did not converge. In contrast, models with fixed UPG and estimated inbreeding or random UPG converged and resulted in similar GEBV. The inclusion of an extra fixed effect in the model made the GEBV unbiased and reduced the inflation. Genomic predictions with MF were slightly biased and inflated due to the unbalanced number of observations assigned to each metafounder. When combining local and external populations, the greatest accuracy can be obtained by adding an extra fixed effect to account for the origin of parents plus UPG with estimated inbreeding or random UPG. To estimate the accuracy, the LR method is more consistent among scenarios, whereas the predictive ability greatly depends on the model specification.  相似文献   

Intestinal antiinflammatory activity of a lyophilized infusion of Turnera ulmifolia in TNBS rat colitis     

Galvez J  de Souza Gracioso J  Camuesco D  Galvez J  Vilegas W  Monteiro Souza Brito AR  Zarzuelo A 《Fitoterapia》2006,77(7-8):515-520

Turnera ulmifolia is a plant popularly known in Brazil and South America as chanana. Some species of Turnera are widely used in folk medicine for different types of inflammatory diseases. In this study, the preventive intestinal antiinflammatory activity of a lyophilized infusion obtained from the aerial parts of T. ulmifolia was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. The results obtained revealed that pretreatment to colitic rats with the extract, at 250 and 500 mg/kg, significantly attenuated the colonic damage induced by TNBS. This beneficial effect was associated with an improvement in the colonic oxidative status, since the infusion prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. On the other hand, this antioxidant activity was confirmed in in vitro studies. In conclusion, the preventive effect exerted by the lyophilized infusion of T. ulmifolia in the TNBS model of rat colitis is probably related to its antioxidant properties, due to its flavonoids content.  相似文献   

Estimates of heritability of atrial fibrillation in the Standardbred racehorse          下载免费PDF全文

M. Kraus  P. W. Physick‐Sheard  L. F. Brito  F. S. Schenkel 《Equine veterinary journal》2017,49(6):718-722

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Participation of phosphofructokinase,malate dehydrogenase and isocitrate dehydrogenase in capacitation and acrosome reaction of boar spermatozoa          下载免费PDF全文

E Breininger  D Dubois  VE Pereyra  PC Rodriguez  MM Satorre  PD Cetica 《Reproduction in domestic animals》2017,52(5):731-740

The aim of this work was to determine the enzymatic activity of phosphofructokinase (PFK), malate dehydrogenase (MDH) and isocitrate dehydrogenase (IDH) in boar spermatozoa and study their participation in bicarbonate‐induced capacitation and follicular fluid‐induced acrosome reaction. Enzymatic activity of these enzymes was determined spectrophotometrically in extracts of boar spermatozoa. Sperm suspensions were incubated in the presence of bicarbonate (40 mM), a well‐known capacitation inducer, or follicular fluid (30%), as an acrosome reaction inducer, and different concentrations of oxoglutarate, oxalomalate and hydroxymalonate, inhibitors of PFK, IDH and MDH, respectively. Capacitation percentages were determined by the fluorescence technique of chlortetracycline (CTC), and true acrosome reaction was determined by trypan blue and differential–interferential contrast, optical microscopy. The activity of PFK in boar spermatozoa enzymatic extracts was 1.70 ± 0.19 U/10¹⁰ spermatozoa, the activity of NAD‐ and NADP‐dependent IDH was 0.111 ± 0.005 U/10¹⁰ and 2.22 ± 0.14 U/10¹⁰ spermatozoa, respectively, and the activity of MDH was 4.24 ± 0.38 U/10¹⁰ spermatozoa. The addition of the specific inhibitors of these enzymes prevented sperm capacitation and decreased sperm motility during capacitation and inhibited the acrosome reaction (AR), without affecting the sperm motility during this process. Our results demonstrate the participation of PFK, IDH and MDH in bicarbonate‐induced capacitation and follicular fluid‐induced acrosome reaction in boar spermatozoa, contributing to elucidate the mechanisms that produce energy necessary for these processes in porcine spermatozoa.  相似文献