Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations     

Antoine LF Gady  Freddy WK Hermans  Marion HBJ Van de Wal  Eibertus N van Loo  Richard GF Visser  Christian WB Bachem 《Plant methods》2009,5(1):13-14

Background  

The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as a model for berry-fruit plants. With the progress of the tomato sequencing project, reverse genetics becomes an obvious and achievable goal.  相似文献   

The Pelagos Sanctuary for Mediterranean marine mammals     

Giuseppe Notarbartolo‐di‐Sciara  Tundi Agardy  David Hyrenbach  Tullio Scovazzi  Patrick Van Klaveren 《水产资源保护:海洋与淡水生态系统》2008,18(4):367-391

• 1. In February 2002, France, Italy and Monaco agreed to establish an international sanctuary for Mediterranean marine mammals. The resulting Pelagos Sanctuary encompasses over 87500 km² of the north‐western Mediterranean Sea, extending between south‐eastern France, Monaco, north‐western Italy and northern Sardinia, and surrounding Corsica and the Tuscan Archipelago.
• 2. The Pelagos Sanctuary illustrates how the tenets of Marine Protected Area (MPA) design can be reconciled with the dynamic nature of oceanic systems, because its spatial scale was defined by oceanographic and ecological considerations, specifically the location of the Ligurian permanent frontal system.
• 3. By expanding protective measures beyond national waters, the Pelagos Sanctuary also sets a precedent for the implementation of pelagic protected areas in the high seas. The Pelagos Sanctuary will contribute to the conservation of the Mediterranean Sea at two scales: (i) locally, by protecting important cetacean foraging and breeding grounds in the Ligurian Sea, and by providing ‘umbrella’ protection to other marine predators in this area; and (ii) regionally, by empowering other conservation measures, such as the Specially Protected Areas Protocol of the Barcelona Convention and the wider goals of the Agreement on the Conservation of Cetaceans of the Black and Mediterranean Seas (ACCOBAMS).
• 4. However, because few cetacean species are resident within the Sanctuary, their effective long‐term conservation will require large‐scale management and coordinated monitoring throughout the Mediterranean basin.

Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

Comparative energy and economic analyses of conventional and System of Rice Intensification (SRI) methods of rice production in Thai Nguyen Province,Vietnam     

Tuyet Thi Anh Truong  John Fry  Phu Van Hoang  Hoang Huy Ha 《Paddy and Water Environment》2017,15(4):931-941

The consumption of energy inputs in agricultural production has been increasing rapidly during the past decades. However, given the limitations and costs of non-renewable energy, increasing production while using the least energy possible has become a major concern of most nations. Prompted by this concern, we conducted a face-to-face survey of 90 farming households in Thai Nguyen Province, Vietnam, to find out how energy is being used in agriculture and, specifically, in their rice production. Through analysis of energy input–output balances, combined with economic efficiency analysis, a comparison was made of conventional and SRI methods of rice production. The study found that applying the SRI method can save around 23% of energy inputs, while increasing energy outputs by 11%. Economic benefits per hectare also rise by more than 8 million dong (USD 364) compared to those under the conventional cultivation system. The study also showed conflicts between the energy and economic balances for manual compared with machine ploughing operations. This study contributes to providing an overview of energy consumption in rice cultivation at the household level. Its findings can help stakeholders to assess current policies and make better decisions on the uses of energy in agricultural production. In addition, the comprehensive approach taken here to analysing energy use and efficiency could expand the analysis and comparison of energy uses at sectoral or activity level—still a new field in Vietnam and many other countries.  相似文献   

Induced expression of selected plant defence related genes in pot azalea, <Emphasis Type="Italic">Rhododendron simsii</Emphasis> hybrid     

Gil Luypaert  Johan Witters  Johan Van Huylenbroeck  Patrick De Clercq  Jan De Riek  Ellen De Keyser 《Euphytica》2017,213(10):227

A set of putative marker genes to study plant defense responses against Polyphagotarsonemus latus, a key pest in the production of Rhododendron simsii hybrids, was selected and validated. Genes belonged to the biosynthetic pathway of phytohormones jasmonic acid (JA) (RsLOX, RsAOS, RsAOC, RsOPR3 and RsJMT) and salicylic acid (SA) (RsPAL and RsICS). Furthermore, RsPPO, a putative marker gene for oxidative stress response was successfully cloned from R. simsii. A CTAB-based extraction protocol was optimized to assure excellent RNA quality for subsequent RT-qPCR analysis. The RT-qPCR protocol was extensively tested and RsRG7 and RsRG14 were selected as reference genes from a geNorm pilot study. Validation of the marker genes was done after application with elicitors [methyl jasmonate (MeJA), coronatine, β-aminobutyric acid and acibenzolar-Smethyl] or wounding. Both 100 μM MeJA and 0.1 μM coronatine had a significant effect on the expression of all marker genes. Foliar application of MeJA on the shoots resulted in a significantly earlier response when compared to root application and subsequent sampling of the shoots. Expression patterns after MeJA treatment were generally the same in six R. simsii genotypes: ‘Nordlicht’, ‘Elien’, ‘Aiko Pink’, ‘Michelle Marie’, ‘Mevrouw Gerard Kint’ and ‘Sachsenstern’. Wounding resulted in the same expression patterns as MeJA treatment except for RsJMT. None of the genotypes showed a significant induction of the latter gene 6 h upon wounding. Findings of these experiments indicated that the tolerant genotype ‘Elien’ has low basal expression levels of RsPPO. This might be the first step towards the breeding of mite-tolerant genotypes.  相似文献   

Multi-model comparison on the effects of climate change on tree species in the eastern U.S.: results from an enhanced niche model and process-based ecosystem and landscape models     

Louis R. Iverson  Frank R. ThompsonIII  Stephen Matthews  Matthew Peters  Anantha Prasad  William D. Dijak  Jacob Fraser  Wen J. Wang  Brice Hanberry  Hong He  Maria Janowiak  Patricia Butler  Leslie Brandt  Christopher Swanston 《Landscape Ecology》2017,32(7):1327-1346

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High vegetative compatibility diversity of Cryphonectria parasitica infecting sweet chestnut (Castanea sativa) in Britain indicates multiple pathogen introductions     

A. Pérez-Sierra  P. Romón-Ochoa  C. Gorton  A. Lewis  H. Rees  S. Van Der Linde  J. Webber 《Plant pathology》2019,68(4):727-737

Chestnut blight, caused by Cryphonectria parasitica, was identified in Devon, UK, in December 2016. Intensive surveys detected the disease at further sites in Devon (seven), Berkshire (one), Dorset (one), Derbyshire (four) and a cluster of eight sites in southeast London. Over 570 survey samples were tested, and 227 were positive for C. parasitica by isolation and real-time PCR. A total of 227 isolates were tested for mating type, and 197 screened for vegetative compatibility group (VCG) and compared with VCGs known from mainland Europe. The same isolates were also screened for the presence of Cryphonectria hypovirus 1 (CHV-1). Eleven VCGs were identified within the UK population. Five corresponded to already known European VCGs but six were unique. The European VCGs mainly came from the Devon, Dorset, Berkshire and Derbyshire disease outbreaks, whilst unique VCGs were almost exclusively from the southeast London cluster. Both mating types were detected, but only one mating type was present at each site, with the exception of a single Devon site. Perithecia of C. parasitica were never observed at any site. CHV-1 was found in seven isolates from three different locations and was always subtype-I, which has limited hypovirulence. Therefore, although CHV-1 is associated with C. parasitica at some outbreaks, it probably has limited impact on virulence. The diversity of VCGs and their distribution at outbreak sites, together with findings of CHV-1, suggests C. parasitica has been introduced to the UK multiple times over at least two decades through international plant trade.  相似文献   

Robotic weeders can improve weed control options for specialty crops     

Steven A Fennimore  Matthew Cutulle 《Pest management science》2019,75(7):1767-1774

Specialty crop herbicides are not a priority for the agrochemical industry, and many of these crops do not have access to effective herbicides. High‐value fruit and vegetable crops represent small markets and high potential liability in the case of herbicide‐induced crop damage. Meanwhile, conventional and organic specialty crop producers are experiencing labor shortages and higher manual weeding costs. Robotic weeders are promising new weed control tools for specialty crops, because they are cheaper to develop and, with fewer environmental and human health risks, are less regulated than herbicides. Now is the time for greater investment in robotic weeders as new herbicides are expensive to develop and few in number, organic crops need better weed control technology and governments are demanding reduced use of pesticides. Public funding of fundamental research on robotic weeder technology can help improve weed and crop recognition, weed control actuators, and expansion of weed science curricula to train students in this technology. Robotic weeders can expand the array of tools available to specialty crop growers. However, the development of robotic weeders will require a broader recognition that these tools are a viable path to create new weed control tools for specialty crops. © 2019 Society of Chemical Industry  相似文献   

Development of enzymes for robust aryloxyphenoxypropionate and synthetic auxin herbicide tolerance traits in maize and soybean crops     

Clayton T Larue  Michael Goley  Lei Shi  Artem G Evdokimov  Oscar C Sparks  Christine Ellis  Andrew M Wollacott  Timothy J Rydel  Coralie E Halls  Brook Van Scoyoc  Xiaoran Fu  Jeffrey R Nageotte  Adewale M Adio  Meiying Zheng  Eric J Sturman  Graeme S Garvey  Marguerite J Varagona 《Pest management science》2019,75(8):2086-2094

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AFLP as a fingerprinting tool for characterising isolates of Fusarium oxysporum f. sp. elaeidis causal organism for fusarium wilt disease of oil palm in Ghana     

Adusei-Fosu  Kwasi  Dickinson  Matthew  Yankey  Egya Ndede 《植物病害和植物保护杂志》2019,126(6):575-584

Journal of Plant Diseases and Protection - Fusarium wilt is a damaging disease of oil palm in Ghana. Genetic diversity of Fusarium oxysporum f. sp. elaeidis (Foe) the causal organism of the disease...  相似文献   

Temperate zone coastal seascapes: seascape patterning and adjacent seagrass habitat shape the distribution of rocky reef fish assemblages     

Swadling  Daniel S.  Knott  Nathan A.  Rees  Matthew J.  Davis  Andrew R. 《Landscape Ecology》2019,34(10):2337-2352

Landscape Ecology - Whilst the composition and arrangement of habitats within landscape mosaics are known to be important determinants of biodiversity patterns, the influence of seascape patterning...  相似文献