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11.
TATSUYA KAJI MASAAKI KODAMA HIROSHI ARAI MASATOMO TAGAWA MASARU TANAKA 《Fisheries Science》2002,68(6):1212-1218
ABSTRACT: Striped bonito Sarda orientalis larvae and juveniles were reared in a laboratory from hatching to 14 days post hatching, and the development of their digestive system was investigated histologically. On day 2 (first feeding day), the larval-type digestive system was differentiated. On day 3, a blind sac with densely distributed gastric glands developed and pyloric ceca appeared, suggesting that the digestive system was attaining adult-type structure. The yolk was completely absorbed on day 4. Bonito larvae preyed on rotifers and/or Artemia nauplii on day 2, but almost the whole gut was occupied by fish larvae from day 4. In the striped bonito, the adult-type digestive system was established right after first feeding, and this precocious development was well synchronized with the appearance of piscivory. 相似文献
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In this study, wind tunnel experiment has carried out to investigate the self staring capability for straight bladed vertical axis wind turbine. The flow visualization has also been performed at the rest of the rotor. The effect of the azimuthal angle of blade position relative to wind direction on the self starting capability was discussed based on the results of flow visualization. The torque and the centripetal force of the rotor when the self starting behavior starts were roughly calculated by using the result of flow visualization of the rotor. It was suggested that there exist the condition of wind speed and configuration of the blade position of the rotor at the rest of rotor to the reach to situational rotation number. 相似文献
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Alexander Smith Camilo A. Beltrán Manabu Kusunoki Alba M. Cotes Keiichi Motohashi Takumasa Kondo Michihito Deguchi 《Journal of General Plant Pathology》2013,79(1):74-85
Twenty-one isolates of Trichoderma spp. were collected from eight states in Colombia and characterized based on the 5′ end of the translation elongation factor-1α (EF1-α1) gene and RNA polymerase II gene encoding the second largest protein subunit (RPB2) by using mixed primers. Seven species of soil-dwelling Trichoderma were found: T. atroviride, T. koningiopsis, T. asperellum, T. spirale, T. harzianum, T. brevicompactum and T. longibrachiatum. Species identifications based on the EF1-α1 gene were consistent with those obtained from the RPB2 gene. Phylogenetic analyses with high bootstrap values supported the validity of the identification of all isolates. These results suggest that using the combination of the genes EF1-α1 and RPB2 is highly reliable for molecular characterization of Trichoderma species. Trichoderma asperellum Th034, T. atroviride Th002 and T. harzianum Th203 prevented germination of more than 70 % of sclerotia of Sclerotinia sclerotiorum in bioassay tests and are promising biological control agents. No relationship between mycelium growth rate and parasitism level was found. 相似文献
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Michihito Tagawa Genya Shimbo Kazuro Miyahara 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(10):1095
The lymphocyte to monocyte ratio (LMR) is a useful prognostic marker of various cancers in human and canine patients. This study aimed to determine whether this ratio could predict disease outcomes in cats with high-grade lymphoma. Medical records of 33 cats diagnosed with high-grade lymphoma were retrospectively analyzed. The prognostic influence of LMR and other clinicopathological data on the time to progression (TTP) and overall survival (OS) was studied using the Kaplan-Meier curves. The optimal cutoff value of this ratio was 3.4, which corresponded to the maximum sensitivity (1.000) and specificity (0.611) of the LMR for predicting median OS days, using receiver operating characteristic analysis. A univariate analysis demonstrated that cats with a low LMR had significant reductions in both TTP [hazard ration (HR) = 3.403, 95% confidence interval (CI): 1.502 to 8.720; P = 0.003] and OS (HR = 3.418, 95% CI: 1.433 to 9.449, P = 0.005). In multivariate analysis, independent predictors of OS included LMR (HR = 2.889, 95% CI: 1.048 to 8.843, P = 0.040), clinical stage (HR = 0.330, 95% CI: 0.118 to 0.960, P = 0.042), and age (HR = 4.151, 95% CI: 1.574 to 11.888, P = 0.004). 相似文献
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Masaki MAEZAWA Ai NAKAMICHI Nao AKIYAMA Michihito TAGAWA Ken-ichi WATANABE Yoshiyasu KOBAYASHI Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1643
A 4-year, 7-month-old Holstein cow presented with anorexia. Physical examination revealed masses in the interscapular region and vagina. Blast cells were detected in the masses and peripheral blood by fine needle aspiration cytology and hematological examination. By bone marrow aspiration, blast cells constituted up to 24.2% of all nucleated cells, and 22% and 2% of non-erythroid cells stained positive for myeloperoxidase and alpha-naphthyl acetate esterase (ANAE), respectively. Pathological examination revealed the mass lesions consisted of a proliferation of tumor cells, which were positive for monocytic markers (HLA-DR and Iba-1). The cow was diagnosed with acute myelomonocytic leukemia (AMML). Even when tumor cells are ANAE-negative, AMML cannot be completely ruled out and should be considered when diagnosing cattle with leukemia/lymphoma. 相似文献
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Hirotaka UNNO Mika INADA Akiyoshi NAKAMURA Michie HASHIMOTO Keiko ITO Koji HASHIMOTO Masaru NIKAIDO Tomohito HAYASHI Eiji HATA Ken KATSUDA Yoshio KIKU Yuichi TAGAWA Kazuhiro KAWAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):1007-1009
A rapid and efficient DNA extraction method was developed for detecting mastitis pathogens in milk. The first critical step involved cell wall disruption by bead-beating, as physical disruption using beads was more effective for DNA extraction from Gram-positive bacteria, such as Staphylococcus aureus, than enzymatic disruption using proteinase K. The second critical step involves the use of acetic acid and ammonium sulfate in the purification process, as these reagents effectively and efficiently remove the lipids and proteins in milk. Using these methods, DNA suitable for loop-mediated isothermal amplification was obtained within 30 min. Also, the rapid and sensitive detection of S. aureus in milk was possible at levels as low as 200 cfu/ml. 相似文献
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Tom ICHINOHE Nobuo KANNO Yasuji HARADA Takuya YOGO Masahiro TAGAWA Satoshi SOETA Hajime AMASAKI Yasushi HARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):761-770
Degenerative cranial cruciate ligament (CCL) rupture is characterized histologically by
degenerating extracellular matrix (ECM) and chondroid metaplasia. Here, we describe the
progression of chondroid metaplasia and the changes in the expression of ECM components in
canine CCL rupture (CCLR). CCLs from 26 stifle joints with CCLR (CCLR group) and normal
CCLs from 12 young beagles (control group) were examined histologically and
immunohistochemically for expression of type I (COLI), type II (COLII), type III collagen
(COLIII) and Sry-type HMG box 9 (SOX9). Cell density and morphology of CCLs were
quantified using hematoxylin–eosin staining. The percentage of round cells was higher in
the CCLR group than in controls. COLI-positive areas were seen extensively in the
connecting fibers, but weakly represented in the cytoplasm of normal CCLs. In the CCLR
group, there were fewer COLI-positive areas, but many COLI-positive cells. The percentages
of COLII-, COLIII- and SOX9-positive cells were higher in the CCLR group than in controls.
The number of spindle cells with perinuclear halo was high in the CCLR group, and most of
these cells were SOX9-positive. Deposition of COLI, the main ECM component of ligaments,
decreased with increased COLIII expression in degenerated CCL tissue, which shows that the
deposition of the ECM is changed in CCLR. On the contrary, expression of SOX9 increased,
which may contribute to the synthesis of cartilage matrix. The expression of COLII and
SOX9 in ligamentocytes showed that these cells tend to differentiate into
chondrocytes. 相似文献
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E. P. STEFFEY J. D. BAGGOT J. H. EISELE‡ N. WILLITS M. J. WOLINER K. A. JARVIS A. R. ELLIOTT M. TAGAWA 《Journal of veterinary pharmacology and therapeutics》1994,17(3):202-210
In monkeys, dogs and swine (six each) we tested the reduction of the isoflurane MAC (minimal alveolar concentration) produced by 2 mg-kg-1 morphine intravenously (i-v.) and the concurrent effect on Pco2 with spontaneous ventilation. MAC fell to a minimum of 55% of control at 53 min in monkeys, 50% at 38 min in dogs and 13% at 33 min in swine. Paco2 rose at constant MAC with morphine to 55–60 mmHg, but did not fall over the next several hours despite the decline of plasma morphine concentration, and the resulting needed rise in isoflurane concentration to keep the anaesthesia depth at 1 MAC. After isoflurane concentration had returned to pre-morphine control levels, naloxone immediately reduced Paco2 to or below control level. Morphine pharmacokinetics in the three species studied conformed to a two-compartment model. 相似文献