柔嫩艾美耳球虫MAGL基因的克隆及表达     

孟祥龙  廖申权  戚南山  吴彩艳  吕敏娜  李娟  孙铭飞  李国清 《畜牧与兽医》2013,45(9):1-5

酰基甘油酯酶(MAGL)是将酰基甘油分解为甘油和游离脂肪酸的丝氨酸水解酶家族成员之一,在酯代谢中起着关键酶的作用,是研制抗鸡球虫药物的重要靶标。本研究利用生物信息学技术预测拼接了柔嫩艾美耳球虫MAGL基因序列,以第二代裂殖子总RNA为模板,通过RTPCR技术获得Etmagl基因。将Etmagl与pCold-43a载体连接,构建pCold-43a-Etmagl重组载体,并在大肠杆菌BL21中获得可溶性蛋白,经亲和层析获得纯化的重组蛋白。结果显示,扩增的Etmagl序列ORF长1 752 bp,编码584个氨基酸,与预测序列相似度为99%,与弓形虫MAGL相似度好(55%);IPTG诱导后融合蛋白高效表达,大小约为114 ku,经免疫印迹鉴定为目的蛋白。本研究成功利用大肠埃希菌原核表达体系重组表达并纯化了柔嫩艾美耳球虫酰基甘油脂肪酶,为建立以MAGL为靶标的抗球虫药物筛选模型奠定了基础。  相似文献   

Morphology and aspects of growth of a trypanosome transmitted by the marine leech Johanssonia arctica (Piscicolidae) from Northern Norway     

Karlsbakk E  Haugen E  Nylund A 《Folia parasitologica》2005,52(3):209-215

The fish leech Johanssonia arctica (Johansson, 1898) was collected from king crabs Paralithodes camtschaticus (Tilesius, 1815) in Finnmark, N Norway, and allowed to feed on experimental fish hosts in the laboratory. This leech ingested blood from laboratory-reared cod (Gadus morhua) and halibut (Hippoglossus hippoglossus). Some experimental halibut acquired trypanosome infection, with parasitaemia between ca. 500 and 60,000 trypanosomes ml(-1). The trypanosomes were of variable size and measured 39-90 microm (mean 57 microm) ca. 81 days post-infection. Characteristic features are cell striation, refractile cytoplasmic granules, anterior nucleus and a relatively long (ca. 6 microm, max 9 microm) distance from the posterior end to the kinetoplast. Following growth, the trypanosomes became increasingly slender, with fewer striae and a nucleus position less pronounced anterior. The trypanosome is considered distinct from a type transmitted by the leech Calliobdella nodulifera (Malm, 1863) in the NE Atlantic, but is regarded conspecific with a trypanosome transmitted by J. arctica in the NW Atlantic. This trypanosome has in the past been identified as Trypanosoma murmanensis Nikitin, 1927, a poorly described species. T. murmanensis cannot be recognized with certainty among the trypanosomes transmitted by C. nodulifera and J. arctica respectively. We propose that the J. arctica-transmitted species is considered T. murmanensis Nikitin, 1927 sensu stricto.  相似文献   

Potential hazard characteristics of Tilia, Betula, and Acer trees removed in the Helsinki City Area during 2001–2003     

Minna Terho  Anna-Maija Hallaksela 《Urban Forestry & Urban Greening》2005,3(2):113-120

In order to study decay, and to improve the management and protection of old urban trees, a total of 256 felled urban trees were examined during 2001–2003: 95 Tilia spp., 74 Betula spp., and 87 Acer spp. Most of the trees (73%) were located in the main parks and along the main streets in the downtown area of Helsinki City, Finland. The mean age of the trees was over 60 years, and the majority (64%) were old park trees. Poor condition and increasing risk of failure were the main reasons for felling in 82% of the cases. Thirty three percent of these trees were degenerated or dead, but the amenity value of 14% of the risk trees was still high. The latter were old, big trees which posed a potential hazard, but had a vital and balanced crown.Some characteristic profiles for potential failure were identified for each of the tree species studied: Ganoderma lipsiense in the butts and hollows in the stems of Tilia spp., weak fork formations together with Rigidoporus populinus on Acer spp., and degeneration together with decay in the stem on Betula spp.Decay fungi most commonly identified were R. populinus, G. lipsiense, Inonotus obliquus and Piptoporus betulinus. In addition, Kretzschmaria deusta was very common in three of the parks, and on every one of the tree species investigated.  相似文献   

Extraesophageal reflux and reflux aspiration in dogs with respiratory diseases and in healthy dogs     

Sirkku Kouki  Sanna J. Viitanen  Ninna Koho  Henna P. Laurila  Liisa Lilja-Maula  Saila Holopainen  Mikko Neuvonen  Mikko Niemi  Aline Fastrès  Cécile Clercx  Minna M. Rajamäki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):268-276

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Increases in volatile organic compound emissions of Scots pine in response to elevated ozone and warming are modified by herbivory and soil nitrogen availability     

Minna Kivimäenpää  Rajendra P. Ghimire  Sirkka Sutinen  Elina Häikiö  Anne Kasurinen  Toini Holopainen  Jarmo K. Holopainen 《European Journal of Forest Research》2016,135(2):343-360

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The marine herring myxozoan Ceratomyxa auerbachi (Myxozoa: Ceratomyxidae) uses Chone infundibuliformis (Annelida: Polychaeta: Sabellidae) as invertebrate host     

Køie M  Karlsbakk E  Nylund A 《Folia parasitologica》2008,55(2):100-104

Sequencing of SSU rDNA showed that actinospores of the tetractinomyxon type, which develop in Chone infundibuliformis Kr?yer (Annelida, Polychaeta, Sabellidae) from the northern ?resund, Denmark, are identical with Ceratomyxa auerbachi Kabata, 1962 (Myxozoa, Ceratomyxidae). This myxosporean was found in the gallbladder of the Atlantic herring Clupea harengus L. from the northern ?resund, Denmark, and from the Bergen area, western Norway. The pansporocysts and actinospores of C. auerbachi are described. This is the third elucidated two-host life cycle of a marine myxozoan, and the first involving a marine ceratomyxid.  相似文献   

不同播期对紫花苜蓿生长性状及草产量的影响   总被引：1，自引：0，他引：1  

张晓华 张众徐娜  庞敏娜 《中国农学通报》2011,27(14):27-30

2009年在内蒙古鄂尔多斯赛乌素嘎查,采用随机区组法进行了不同播期对紫花苜蓿生长性状及草产量影响的田间试验研究。连续2年对出苗时间、出苗率、越冬率、株高、物候期、草产量、鲜干比、茎叶比的测定结果表明,在春播与秋播区间内,出苗时间与播种日期存在显著的正相关关系,生育时期随播期的提前而相应提前;秋播越早,根颈越粗,入土越深,当年越冬性越强,翌年草产量也越高;不同播期对鲜干比、茎叶比影响甚微。综合评价认为,该地种植紫花苜蓿,宜选择秋季播种,阈值为9月中旬。  相似文献   

Early detection of ketoprofen-induced acute kidney injury in sheep as determined by evaluation of urinary enzyme activities     

Raekallio MR  Saario-Paunio EM  Rajamäki MM  Sankari SM  Palviainen MJ  Siven MS  Peltoniemi SM  Leinonen ME  Honkavaara JM  Vainio OM 《American journal of veterinary research》2010,71(10):1246-1252

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Estimating annual GPP,NPP and stem growth in Finland using summary models     

Sanna Härkönen  Minna Pulkkinen  Remko Duursma  Annikki Mäkelä 《Forest Ecology and Management》2010

In this study we introduce and test a new simple approach for estimating annual stand-level gross primary production (GPP), net primary production (NPP) and stem biomass growth based on carbon acquisition and allocation, by combining existing summary models. The focus is on the variation of GPP and NPP across different parts of Finland caused by climate.  相似文献   

Modelling the predisposition of Norway spruce to <Emphasis Type="Italic">Ips typographus</Emphasis> L. infestation by means of environmental factors in southern Finland     

Minna Blomqvist  Maiju Kosunen  Mike Starr  Tuula Kantola  Markus Holopainen  Päivi Lyytikäinen-Saarenmaa 《European Journal of Forest Research》2018,137(5):675-691

New measures for effective monitoring and controlling of bark beetle infestations are needed as a response to intensified outbreaks caused by the climate change. Various environmental factors affect tree health and susceptibility, as well as stand predisposition to bark beetles. European spruce bark beetle Ips typographus L. abundance and outbreak frequency in Finland has significantly increased during the last decade. The ability to identify sites under a high risk of infestation would facilitate adaptation to this new situation and help target limited forest health management resources. Accordingly, our goal was to investigate the importance of various stand, soil and topographic characteristics in the assessing predisposition of Norway spruce dominated urban forest in southern Finland to I. typographus infestations. Information on the environmental factors was assessed in the field in 2014 and derived from a digital elevation model. Ips typographus infestation intensity was classified into three infestation index classes based on tree-wise symptoms of resin flow, discoloration and defoliation. Cumulative logit link models were utilized for investigating stand-level infestation probability. The best explanatory factors were aspect, slope, site type and soil texture. Models with the highest cumulative probabilities for severe infestation were linked with eastern aspect, moderate steep slope and rich site type fertility (0.72) and eastern aspect, shallow soil and rich site type fertility (0.71). Higher soil C/N ratios with east aspect and rich site type fertility was associated with an increased risk of severe infestation in a third model. The lowest risk was associated with southern and southwestern aspects, fine soil texture, moderate site fertility and gentle slopes.  相似文献