Effect of Bovine Ovarian Tissue Vitrification on the Structural Preservation of Antral Follicles     

MS Faheem  I Carvalhais  E Baron  F Moreira da Silva 《Reproduction in domestic animals》2013,48(5):774-780

This study was performed to evaluate the structural preservation of antral follicles after bovine ovarian tissue vitrification using histological analysis. Ovaries (n = 30) of slaughtered cows were cut into small fragments using a scalpel blade, and the ovarian tissues were randomly assigned to vitrification using 15% dimethyl sulphoxide (DMSO) and 15% ethylene glycol (EG) and fresh tissues (control) groups. For histological evaluations, fresh and post‐thawing ovarian tissues were immediately fixed, serially sectioned into 5‐μm sections and stained with haematoxylin and eosin (H&E). Nine serial sections per fragment were subjected for morphological assessment. The diameter of the antral follicles was determined and classified into four groups: 1 (≤1 mm), 2 (>1–2 mm), 3 (>2–3 mm) and 4 (>3–4 mm). Then, follicular morphology was evaluated in relation to atresia and categorized into seven grades: Grade A (healthy follicle); Grades B, C and D (early atresia); Grades E and F (moderate atresia); and Grade G (advanced atresia). The results revealed that small diameters of antral follicles (1 and 2 mm) were more susceptible for cryoinjury. The normal follicular morphology (Grade A) was not affected by vitrification throughout follicle diameters. Nevertheless, some damage features were monitored after vitrification. In conclusion, the morphological structure of bovine antral follicles could be successfully preserved by ovarian tissue vitrification.  相似文献   

An Association Analysis Between a Silent C558T Polymorphism at the Pig Vascular Cell Adhesion Molecule 1 Locus and Sow Reproduction and Piglet Survivability Traits     

O Ramirez  A Tomàs  J Casellas  M Blanch  JL Noguera  M Amills 《Reproduction in domestic animals》2008,43(5):542-546

The vascular cell adhesion molecule 1 (VCAM1) has a strong influence on embryonic development and on the formation of the umbilical cord and placenta. These developmental processes are crucial to ensure the success of pregnancy. In this work, we have identified two T306A and C558T single nucleotide polymorphisms (SNP) at exons 2 and 3 of the pig VCAM1 locus, respectively. The T306A substitution involves a non conservative Asn to Lys replacement at amino acid position 102, whereas the C558T polymorphism is synonymous. An in silico prediction of the consequences of the Asn₁₀₂→Lys₁₀₂ mutation with the PolyPhen software revealed that it is not deleterious. The T306A SNP segregated in the Iberian, Piétrain, Duroc, Large White and Landrace breeds as well as in European wild boars. The C558T SNP also segregated and most of commercial standard breeds. The genotyping of the C558T SNP in an Iberian × Meishan intercross allowed to find a suggestive association (Bonferroni threshold, p < 0.004) between C558T genotype and time the newborn piglet needs to reach the udder (p = 0.013) as well as a significant one with time to make the first ingestion of colostrum (p = 0.003). The biological basis of these associations remains unclear and they should be interpreted with caution.  相似文献   

Bovine Oocyte Quality in Relation to Ultrastructural Characteristics of Zona Pellucida,Polyspermic Penetration and Developmental Competence     

P Santos  A Chaveiro  N Simões  F Moreira da Silva 《Reproduction in domestic animals》2008,43(6):685-689

In the present study, the effect of bovine oocyte quality related to ultrastructural characteristics of zona pellucida (ZP), polyspermic penetration and embryo developmental competence was evaluated. Cumulus–oocyte complexes were punctioned from 453 ovaries, classified as 1, 2, 3 and 4 according to their morphological aspect, matured for 24 h and then divided into two groups. In group A, oocytes were fixed in 2.5% glutaraldehyde and 0.1 m sodium cacodylate and examined under a scanning electron microscope. Photomicrographs were taken and ZP’s pores were evaluated in squares of 6.4‐μm width. In group B, oocytes were fertilized in vitro. After 48 h, non‐cleaved oocytes were fixed for polyspermy evaluation. On days 7, 9 and 10, embryos were classified as developed (blastocysts and hatched blastocysts). Results showed that quality 1 oocytes revealed a ZP pore diameter of 0.50 ± 0.07 μm, which was smaller than the observed on oocytes of quality 2 (0.83 ± 0.10 μm), quality 3 (1.02 ± 0.22 μm) and quality 4 (1.38 ± 0.59 μm) (p ≤ 0.05). For In Vitro Fertilization (IVF), results showed that embryos originating from oocytes classed as 3 and 4 had lower cleavage rate (68.4% and 43.8%) than those belonging to class 1 and 2 (79.5% and 69.3%) (p ≤ 0.05). None oocyte classified as 3 and 4 developed to hatch blastocysts, while for oocytes belonging to quality 1 and 2, these values were, respectively, 15.2% and 12.5%. Concerning polyspermy, oocytes class 1 and 2 had lower polyspermic penetration than those belonging to class 3 and 4 (respectively 4.1%, 4.5%, 11.1% and 9.8%, for class 1, 2, 3 and 4). In conclusion, the present study demonstrated that oocytes with low qualities result in lower developmental competence and with high percentage of polyspermy after IVF, which can be the result of the ZP structure such as the number and the pore’s diameter.  相似文献   

Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos     

Luiz Sergio Almeida Camargo  Fernanda Queiros Costa  Michele Munk  Sabine Wohlres‐Viana  Raquel Varela Serapio  Bruno Campos Carvalho  Paulo Henrique Campos  Alex Cabral Vieira  Luiz Altamiro Garcia Nogueira  Joao Henrique Moreira Viana 《Reproduction in domestic animals》2019,54(10):1357-1365

This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus–oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleavage at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes.  相似文献   

Behavioral and physiological evaluation of sows raised in outdoors systems in the Brazilian semiarid region     

de Melo  Rafaela Leitão Correia  Dutra Júnior  Wilson Moreira  Palhares  Liliane Olímpio  de Moura Ferreira  Débora Nathália  de Aquino  Rafael Santos  Cordeiro Manso  Helena Emília Cavalcanti 《Tropical animal health and production》2019,51(5):1057-1063

Tropical Animal Health and Production - The objective of this study was to evaluate the biochemical, behavioral, and physiological parameters in pregnant sows of native and improved crossbreeds...  相似文献   

Intranasal Osteosarcoma in a Dog—A Case Report     

Gabriela Noronha de Toledo  Pamela Rodrigues Reina Moreira  Daniele Santos Rolemberg  Andréia Coutinho Facin  Julio Edward Hough Monteiro  Mareliza Possa de Menezes  Maria Eduarda Bastos Andrade Moutinho da Conceição  Felipe Augusto Ruiz Sueiro  Rosemeri de Oliveira Vasconcelos  Paola de Castro Moraes  Mirela Tinucci Costa  Andrigo Barboza de Nardi 《Topics in companion animal medicine》2018,33(2):35-39

Osteosarcoma is the most commonly reported bone tumor in dogs, typically affecting the axial and appendicular skeleton. Involvement of the paranasal sinuses and the nasal cavity is extremely rare. This report describes a 6-year-old, female, cross-breed dog presented with signs of 90 days duration and diagnosed with a mass located in the right nasal cavity. Radiography, rhinoscopy, and computed tomography findings suggested the presence of an intranasal tumor. Osteosarcoma in the nasal cavity was diagnosed based on histopathological examination and clinical and imaging findings. Survival time was 12 months from diagnosis.  相似文献   

Direct measurement of femtomoles of osmium and the 187Os/186Os ratio in seawater     

S Levasseur  JL Birck  CJ Allegre 《Science (New York, N.Y.)》1998,282(5387):272-274

Two depth profiles of the osmium concentration and the 187Os/186Os isotopic ratio in the Indian Ocean showed that the osmium concentration seems to be unaltered by chemical or biological processes occuring in seawater; accordingly, osmium is conservative. These data were obtained from an experimental method that eliminated the problems related to osmium preconcentration. This method led to a new evaluation of the concentration of osmium in seawater; the mean concentration of osmium and the 187Os/186Os ratio are equal to 10.86 +/- 0.07 picograms per kilogram and 8.80 +/- 0.07, respectively. The results suggest the existence of an organocomplex that dominates the speciation of osmium in seawater.  相似文献   

Unconditional quantum teleportation   总被引：5，自引：0，他引：5  

A Furusawa  JL Sorensen  SL Braunstein  CA Fuchs  HJ Kimble  ES Polzik 《Science (New York, N.Y.)》1998,282(5389):706-709

Quantum teleportation of optical coherent states was demonstrated experimentally using squeezed-state entanglement. The quantum nature of the achieved teleportation was verified by the experimentally determined fidelity Fexp = 0.58 +/- 0.02, which describes the match between input and output states. A fidelity greater than 0.5 is not possible for coherent states without the use of entanglement. This is the first realization of unconditional quantum teleportation where every state entering the device is actually teleported.  相似文献   

Femtosecond activation of reactions and the concept of nonergodic molecules     

EWG Diau  JL Herek  ZH Kim  AH Zewail 《Science (New York, N.Y.)》1998,279(5352):847-851

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Tracking the long-term decline and recovery of an isolated population     

RL Westemeier  JD Brawn  SA Simpson  TL Esker  RW Jansen  JW Walk  EL Kershner  JL Bouzat  KN Paige 《Science (New York, N.Y.)》1998,282(5394):1695-1698

Effects of small population size and reduced genetic variation on the viability of wild animal populations remain controversial. During a 35-year study of a remnant population of greater prairie chickens, population size decreased from 2000 individuals in 1962 to fewer than 50 by 1994. Concurrently, both fitness, as measured by fertility and hatching rates of eggs, and genetic diversity declined significantly. Conservation measures initiated in 1992 with translocations of birds from large, genetically diverse populations restored egg viability. Thus, sufficient genetic resources appear to be critical for maintaining populations of greater prairie chickens.  相似文献