Rigid removable cover for dorsal wound protection and tube fixation in pigs          下载免费PDF全文

GD Stynes  GK Kiroff  WA Morrison  GA Edwards  RS Page  MA Kirkland 《Australian veterinary journal》2016,94(4):111-116

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Systemic Concentrations of Endogenous and Exogenous FSH in Anoestrous Ewes Superstimulated with Folltropin^®-V     

PM Bartlewski  KJ Fuerst  BD Alexander  WA King 《Reproduction in domestic animals》2009,44(2):353-358

The synchronization of follicular waves with medroxyprogesterone acetate (MAP) and oestradiol‐17β (E₂‐17β) prior to ovarian superstimilation in anoestrous ewes reduces the variability in superovulatory responses by an unknown mechanism. Follicle stimulating hormone (FSH) is a primary promoter of antral follicular development, but the relevance of circulating FSH concentrations to the superovulation performance in ewes has not been examined. Eighteen anoestrous Rideau Arcott ewes (May–June) were superovulated with Folltropin^®‐V (porcine FSH), with (n = 8; treated ewes) or without (n = 10; control ewes) a single i.m. dose of 350 μg of E₂‐17β, given on the sixth day of a 14‐day treatment with MAP‐releasing intravaginal sponges (60 mg). The superovulatory treatment, begun 6 days after E₂‐17β injection, consisted of six i.m. applications of Folltropin^®‐V given twice daily (at 08:00 and 16:00 h), followed by an i.m. injection of GnRH (50 μg). Blood samples collected every 8 h throughout the 3‐day treatment, were analysed by radioimmunoassays for concentrations of ovine and porcine FSH, using species‐specific standards and primary antibodies. Serum concentrations of oFSH were greater (p < 0.05) in the controls compared to treated ewes at 40, 64 and 72 h and the variability in mean oFSH concentrations was greater (p < 0.05) in control ewes at 40, 48, 64 and 72 h after the 1^st Folltropin^®‐V injection. There were no differences (p > 0.05) between the two groups in serum concentrations of pFSH. Significant correlations were recorded between the number of corpora lutea (CL) and oFSH concentrations at 8 h (r = 0.72, p < 0.05), 16 h (r=0.63, p < 0.05) and 64 h (r = 0.84, p < 0.01) after the 1^st Folltropin^®‐V injection. The total number of recovered embryos was positively correlated to oFSH concentrations at 56 h (r = 0.69, p < 0.05). We concluded that changes in endogenous FSH concentrations during ovarian superstimulation with pFSH might contribute to the variability in superovulatory responses in ewes.  相似文献   

Dukes'' Physiology of Domestic Animals     

WA Snowdon 《Australian veterinary journal》1994,71(6):187-187

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Different Culture Media Requirements of IVF and Nuclear Transfer Bovine Embryos   总被引：3，自引：0，他引：3  

GF Mastromonaco  E Semple  C Robert  GJ Rho  DH Betts  WA King 《Reproduction in domestic animals》2004,39(6):462-467

Important differences exist between in vitro fertilized (IVF) and nuclear transfer (NT) bovine embryos. Studies have shown that although in vitro development is comparable, post-implantation survival is greatly reduced in NT embryos. In this study, we compare serum and bovine serum albumin (BSA) supplementation during oocyte maturation and embryo culture of IVF and NT embryos. In experiment 1, oocytes and embryos were randomly distributed into different treatment groups consisting of synthetic oviductal fluid (SOF) medium supplemented with either serum, fatty acid-free BSA (FAF) or fraction V BSA during maturation and/or culture to assess IVF embryo development. In experiment 2, oocytes were matured in SOF + serum or SOF + FAF and reconstructed embryos were cultured in SOF + FAF to assess NT embryo development. Among the IVF treatment groups, a greater number of blastocysts were observed in the steer serum (SER) group (IVM and IVC in SOF + serum) on day 6; however, no significant differences were seen in blastocyst development from day 8 onwards. Hatching frequencies on days 8 and 9 were significantly greater in groups with serum, with the exception of FAF (IVM and IVC in SOF + FAF) on day 9. For the NT treatment groups, the presence of serum during IVM resulted in a higher proportion of MII oocytes and increased blastocyst development and hatching rates were compared with supplementation of FAF. These results indicate that both serum and FAF provide comparable embryo development for IVF but not for NT bovine embryos.  相似文献   

留茬高度对人工草地牧草产量及质量的影响   总被引：14，自引：5，他引：9  

瓦庆荣  代志进  卢琪 《草业学报》2000,9(1):65-68

试验对黑麦草／白三叶混播草地在不同留茬高度下的生产特性进行了分析研究,研究结果表明,草地牧草的枯死率与留茬高度呈正比关系,在不同留茬高度下,草地牧草青绿物质的生长速度和产量呈抛物线形式变化;青绿牧草产量占牧草总产量的百分比随留茬高度的上升而下降,而死亡物质的数量占牧草产量的百分比随留茬高度的上升而增加。  相似文献   

Treatment of feline hyperthyroidism using orally administered radioiodine: a study of 40 consecutive cases     

R. MALIK  WA LAMB  DB CHURCH 《Australian veterinary journal》1993,70(6):218-219

SUMMARY Forty cats with hyperthyroidism were treated using 200 to 300 (typically 250) mBq of orally administered ¹³¹I. Thirty-six cases (90%) were successfully treated, as assessed by resolution of clinical signs and reduction In plasma thyroxine concentrations to normal or reduced values after treatment. Although higher doses of ¹³¹I appear to be required when the radioisotope is administered orally rather than Intravenously, a less stressful administration procedure and greater availability of therapy capsules offer useful advantages for treating thyrotoxic cats.  相似文献   

Hyperthermia in Merino wethers affected with humpyback disease     

BHG PEARSE  SKJ PEUCKER  WA HOEY 《Australian veterinary journal》1992,69(4):94-95

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贵州优良草坪草种质资源评价   总被引：1，自引：1，他引：0  

尚以顺  吴佳海  瓦庆荣  赵熙贵  刘贵林  张文 《草业科学》2003,20(1):26-28

通过对1998－2000年引种征集的19个草坪草品种特性试验以及对供试草种的密度、质地、色泽、均一性、抗性等进行测定，分析表明：参试的19个草坪草品种，大多数在独山地区的气候、土壤等自然条件下生长良好，是较理想的草坏草，可供生产上利用。  相似文献   

Pyelonephritis, nephrolithiasis and perinephric abscessation in a dog     

DC LEWIS  DRT ADAMSON  KA JACOBS  WA LAMB 《Australian veterinary journal》1988,65(6):195-196

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Chronological Appearance of Apoptosis in Bovine Embryos Reconstructed by Somatic Cell Nuclear Transfer from Quiescent Granulosa Cells     

JO Gjørret  J Wengle  P Maddox-Hyttel  WA King 《Reproduction in domestic animals》2005,40(3):210-216

Efficiency of cloning has remained low and in spite of attempts to improve this technology, many reconstructed embryos do not implant or are lost during early pregnancy. Chromosomal aberrations, deviant gene expression patterns and abnormal regulation of cell death may be involved in this increased early embryonic loss. Here, we investigate the chronological onset of both apoptotic changes in nuclear morphology and DNA degradation [detected by transferase-mediated dUTP nick-end labelling (TUNEL) reaction] in bovine two-cell- to blastocyst-stage embryos. Such embryos were generated either by reconstruction with nuclear transfer from quiescent granulosa cells or by regular in vitro embryo production. Nuclear condensation was observed from the two-cell stage and TUNEL labelling was observed from the six-cell stage in reconstructed embryos, whereas nuclear condensation was evident from the eight-cell stage and TUNEL labelling from the 13-cell stage in embryos derived in vitro. Furthermore, reconstructed embryos displayed elevated ratios of embryos containing apoptotic nuclei at pre-compaction stages and higher indices of apoptotic nuclei in morula and blastocyst stages when compared with in vitro-produced embryos.  相似文献