Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification     

Parel JD  Galula JU  Ooi HK 《Veterinary parasitology》2008,153(3-4):379-383

To differentiate the morphologically similar pinworms of the common laboratory rodents, such as Syphacia obvelata and Syphacia muris, we amplified and sequenced the region spanning the internal transcribed spacer 1 (ITS-1), 5.8S gene, and ITS-2 of the ribosomal DNA followed by designing of species-specific primers for future use in the identification of the worms. It was observed that S. obvelata, S. muris and Aspiculuris tetraptera can be differentiated from each other based on their rDNA sequences. This is the first report of the ITS-1, 5.8S, and ITS-2 of the rDNA of the three aforementioned rodent pinworm species. The use of restriction endonucleases, AluI or RsaI, further allowed the delineation of the three species. Moreover, we also constructed species-specific primers that were designed for unique regions of the ITS-2 of the three species. This approach allowed their specific identification with no amplicons being amplified from heterogenous DNA samples, and sequencing confirmed the identity of the sequences amplified. Thus, the use of these specific primers along with PCR-RFLP can serve as useful tools for the identification of pinworms in rats, mice, and wild rodents.  相似文献   

Comparison of microfilaria concentration method for Setaria digitata infection in cattle and for Dirofilaria immitis infection in dogs     

Watanabe Y  Yang CH  Tung KC  Ooi HK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):543-545

Several peripheral blood microfilaria concentration methods that use Acetone (Acetone test), 2% formalin (modified Knott method), 5% Tween 20 solution, distilled water, 1% or 0.1% SDS were compared for their efficacy in detecting Setaria digitata microfilaria in cattle. The Acetone test was found to be more efficacious than the modified Knott method or the 5% Tween 20 solution test for detecting the S. digitata microfilaria in bovine blood. However, besides the Acetone test, the modified Knott method was also found to be suitable for Dirofilaria immitis microfilaria detection in dogs. SDS and distilled water were found not to be effective as hemolytic agent for the disruption of the red blood cell of both the cattle and dogs. Thus, the Acetone test is recommended for the primary screening of microfilaremia of S. digitata in cattle.  相似文献   

Epigenetic decisions in mammalian germ cells   总被引：1，自引：0，他引：1  

Schaefer CB  Ooi SK  Bestor TH  Bourc'his D 《Science (New York, N.Y.)》2007,316(5823):398-399

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Inheritance of seed resistance to bruchids in cultivated mungbean (Vigna radiata, L. Wilczek)     

Prakit Somta  Chanida Ammaranan  Peter A. -C. Ooi  Peerasak Srinives 《Euphytica》2007,155(1-2):47-55

Bruchid beetles or seed weevils are the most devastating stored pests of grain legumes causing considerable loss to mungbean (Vigna radiata (L.) Wilczek). Breeding for bruchid resistance is a major goal in mungbean improvement. Few sources of resistance in cultivated genepool were identified and characterized, however, there has been no study on the genetic control of the resistance. In this study, we investigated the inheritance of seed resistance to Callosobruchus chinensis (L.) and C. maculatus (F.) in two landrace mungbean accessions, V2709BG and V2802BG. The F₁, F₂ and BC generations were developed from crosses between the resistant and susceptible accessions and evaluated for resistance to the insects. It was found that resistance to bruchids in seeds is controlled by maternal plant genotype. All F₁ plants derived from both direct and reciprocal crosses exhibited resistance to the bruchids. Segregation pattern of reaction to the beetles in the F₂ and backcross populations showed that the resistance is controlled by a major gene, with resistance is dominant at varying degrees of expressivity. Although the presence of modifiers was also observed. The gene is likely the same locus in both V2709BG and V2802BG. The resistant gene is considered very useful in breeding for seed resistance to bruchids in mungbean.  相似文献   

C-protein (MyBP-C) isoforms from carp ordinary and dark muscles and muscle type-specific binding to myosin     

Tsuyoshi Okagaki  Ryo Suzuki  Atsushi Ooi 《Fisheries Science》2007,73(3):640-650

ABSTRACT:   C-protein is a myosin-associated protein of vertebrate striated muscle, and its function and properties have been extensively examined. However, there has been no report of C-protein of fish skeletal muscle so far. C-protein was identified in carp skeletal muscle by immunoassay using antibody against chicken C-protein, and the muscle-type specific C-protein was purified from carp ordinary and dark muscles for the first time. Although C-protein could be prepared from crude myosin by the reported procedure, C-protein degraded appreciably during the purification steps. Accordingly, C-protein was selectively extracted from the muscle with 0.15 M K-phosphate buffer (pH 5.8), and purified by ammonium sulfate fractionation, followed by AF-blue chromatography. Myosin free from the accessory proteins was obtained by diethylaminoethyl (DEAE) chromatography and used to assay the binding of C-protein with myosin. Ordinary muscle C-protein bound to ordinary muscle myosin in a saturable manner, but its maximum amount of binding was approximately twice that of dark muscle myosin. Similarly, dark muscle C-protein bound to dark muscle myosin much more than to ordinary muscle myosin. These results suggest that C-protein isoforms specifically bound with myosin isoforms originated from the same type of muscle.  相似文献   

A survey of abomasal and duodenal nematodes in cattle in Hokkaido, Japan     

Y Oku  M Nakazawa  S Hatakeyama  S Miyaji  T Kitaguchi  C A Cabrera-Lopez  M Okamoto  M Kamiya  M Ohbayashi  H K Ooi 《The Japanese journal of veterinary research》1987,35(2):67-72

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Assessment of Genetic Variability in the Coding Sequence of Melatonin Receptor Gene (MTNR1A) in Tropical Arid Sheep Breeds of India          下载免费PDF全文

VK Saxena  BK Jha  AS Meena  HK Narula  D Kumar  SMK Naqvi 《Reproduction in domestic animals》2015,50(3):517-521

Seasonal behaviour in sheep, which varies in tropical and temperate environmental conditions, is a matter of study, because it can provide a clue to address the problem of seasonality in sheep. Melatonin receptor is the membrane‐bound G‐coupled receptor, sensing the message of photoperiodic cues thorough melatonin. Restriction fragment length polymorphism (RFLP) studies were carried out to assess the variability of gene at G612A and C606T SNPs in MTNR1A gene, which have been studied to be markers for out‐of‐season breeding. Allelic frequency distribution corresponded to higher frequency of GG and CC genotype, in tropical arid sheep breed in comparison with temperate region sheep breed. PCR amplification of MTNR1A gene of 30 animals was performed and single nucleotide polymorphisms (SNPs) identification was carried out using Lasergene software. Seven SNPs/mutations were identified, but most of them were synonymous, except the one G706A, leading to substitution of valine by isoleucine. Polyphen‐2 analysis of G706A mutation revealed that it is a benign mutation. Two important SNPs C426T and G555A, which were identified in temperate sheep breeds, could not be traced in Magra and Marwari breeds of sheep. Thus, the Magra and Marwari breeds of tropical, arid region demonstrated the presence of both polymorphic SNPs markers G612A and C606T, associated with out‐of‐season breeding. GG and CC genotypes were having a higher prevalence in the studied population.  相似文献   

A protein interaction map of Drosophila melanogaster     

Giot L  Bader JS  Brouwer C  Chaudhuri A  Kuang B  Li Y  Hao YL  Ooi CE  Godwin B  Vitols E  Vijayadamodar G  Pochart P  Machineni H  Welsh M  Kong Y  Zerhusen B  Malcolm R  Varrone Z  Collis A  Minto M  Burgess S  McDaniel L  Stimpson E  Spriggs F  Williams J  Neurath K  Ioime N  Agee M  Voss E  Furtak K  Renzulli R  Aanensen N  Carrolla S  Bickelhaupt E  Lazovatsky Y  DaSilva A  Zhong J  Stanyon CA  Finley RL  White KP  Braverman M  Jarvie T  Gold S  Leach M  Knight J  Shimkets RA  McKenna MP  Chant J  Rothberg JM 《Science (New York, N.Y.)》2003,302(5651):1727-1736

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Fucoxanthin and Its Metabolites in Edible Brown Algae Cultivated in Deep Seawater     

Kanami Mori  Takashi Ooi  Masanori Hiraoka  Naohiro Oka  Hideyuki Hamada  Mitsumasa Tamura  Takenori Kusumi 《Marine drugs》2004,2(2):63-72

Three metabolites of fucoxanthin were isolated from a brown alga, Scytosiphon lomentaria, and the structure of a new compound was determined by NMR. The content of fucoxanthin, a biologically active carotenoid, in four edible brown algae, cultivated in deep seawater, was studied.  相似文献   

Optimal set of microsatellite markers required to detect illegitimate progenies in selected oil palm (Elaeis guineensis Jacq.) breeding crosses     

Siti Hazirah Zolkafli  Maizura Ithnin  Kuang-Lim Chan  Mohd Isa Zainol Abidin  Ismanizan Ismail  Ngoot Chin Ting  Leslie Cheng-Li Ooi  Rajinder Singh 《Breeding Science》2021,71(2):253

Oil palm is continually being improved via controlled crossing of selected palms to ensure sustainable yields and productivity. As such, correct parental assignment is important as the presence of illegitimates will compromise the progress of improvement. In the present study, we determined the optimal number of microsatellite (SSR) markers for detection of illegitimates in selected oil palm crosses with high confidence. Determining the optimal number of markers to assign parentage will ensure that the DNA fingerprinting will be cost effective for routine use as a quality control tool in oil palm improvement programs. Here, we evaluated a wide range of crosses that included a cross derived from wild germplasm palm. The results revealed that markers with high PIC are informative and detect most of the alleles present in a cross, including those exhibited by the illegitimates. A larger number of optimum sets of markers are needed to detect all illegitimates for crosses with higher levels of genetic diversity. The optimal number of polymorphic SSR markers determined in the present study can ensure that appropriate quality control is implemented for oil palm improvement programs.  相似文献