Multiple immunofluorescence labelling enables simultaneous identification of all mature fibre types in a single equine skeletal muscle cryosection     

Tulloch LK  Perkins JD  Piercy RJ 《Equine veterinary journal》2011,43(4):500-503

Skeletal muscle is composed of a heterogeneous mixture of several fibre types, each with specific physiological properties. In equine muscle, identification of these individual fibres (fibre typing) is important for both exercise physiology and pathological studies. Traditionally, fibre typing has been achieved by adenosine triphosphatase (ATPase) histochemistry or by immunoperoxidase labelling with antibodies directed at myosin heavy chain isoforms. ATPase histochemistry can be temperamental and lacks specificity, and both techniques require staining of serial cryosections to reveal the entire fibre type compliment of a single sample, which is time consuming and prone to inaccuracy. Here we describe an immunofluorescence labelling technique that enables rapid, accurate and specific identification of the 3 mature equine muscle fibre types in a single cryosection.  相似文献   

Observations on the indirect transmission of virulent ovine footrot in sheep yards and its spread in sheep on unimproved pasture     

RJ WHITTINGTON 《Australian veterinary journal》1995,72(4):132-134

SUMMARY: Virulent ovine footrot was transmitted accidentally to a group of 23 adult Merino sheep (flock B) after holding for 1 hour in sheep yards, which earlier the same day had contained another flock (flock A) with < 1% prevalence of sheep with footrot lesions. Sheep in flock B were rendered susceptible to virulent footrot by grazing 600 mm high unimproved pasture dominated by paspalum ( Paspalum dilatatum ) and kangaroo grass ( Themeda australis ) during warm, humid and wet weather. In addition to moisture, interdigital abrasions caused by the pasture might have predisposed the interdigital skin to infection with Dichelobacter nodosus .  相似文献   

Restriction-endonuclease analysis of Australian isolates of Leptospira interrogans serovar hardjo from cattle with agalactia and abortion   总被引：1，自引：0，他引：1  

S. DJORDJEVIC  M. HORNITZKY  AD ROSS  RJ WHITTINGTON 《Australian veterinary journal》1993,70(3):98-100

SUMMARY Polyacrylamide gel electrophoresis and agarose gel electrophoresis were used to resolve restriction endonuclease digests of 20 Australian isolates of Leptospira interrogans cultured from urine samples of cattle with agalactia and abortion. The restriction endonuclease profiles of 19 isolates closely matched the profiles of L interrogans serovar hardjo subtype hardjobovis reference strains. The remaining isolate had a different restriction profile from subtype hardjobovis and subtype hardjoprajitno reference strains and was serologically identified as serovar pomona. Silver staining of polyacrylamide gels gave enhanced resolution of restriction fragments compared with the traditional method of ethidium bromide staining of agarose gels.  相似文献   

Investigation of a second focus of equine morbillivirus infection in coastal Queensland   总被引：6，自引：0，他引：6  

RJ ROGERS  IC DOUGLAS  FC BALDOCK  RJ GLAVILLE  KT SEPPANEN  LJ GLEESON  PN SELLECK  KJ DUNN 《Australian veterinary journal》1996,74(3):243-244

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Investigations into the toxicity of Echium plantagineum in sheep: 1. Field grazing experiments     

JT SEAMAN  WS TURVEY  SJ OTTAWAY  AR GILMOUR  RJ DIXON† 《Australian veterinary journal》1989,66(9):279-285

A field grazing trial was undertaken to monitor the health and production of crossbred sheep grazing pasture where Echium plantagineum constituted a considerable proportion of the available forage. The trial, conducted for 19 months over successive grazing seasons, demonstrated a significant difference in production, with sheep on the E. plantagineum pasture being lighter and growing less wool compared with sheep on Echium-free pasture. No mortalities involving pyrrolizidine alkaloid poisoning were recorded in sheep grazing E. plantagineum, although there was histological evidence of moderately severe liver damage associated with high liver copper concentrations in at least one sheep following the grazing of large quantities of the plant.  相似文献   

Comparison of immunogenicity and virulence between Babesia bigemina parasites from continuous culture and from a splenectomised calf   总被引：1，自引：0，他引：1  

WK. JORGENSEN  AJ. DE. VOS  RJ. DALGLIESH † 《Australian veterinary journal》1989,66(11):371-372

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An outbreak of botulism in a deer herd in Queensland     

P. CHAMBERLAIN  RJ THOMAS† 《Australian veterinary journal》1995,72(11):427-428

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Premature farrowings caused by feeding cottonseed meal   总被引：1，自引：0，他引：1  

RJ LOVE  AJ PEACOCK  G EVANS † 《Australian veterinary journal》1990,67(6):223-226

Increasing the level of cottonseed meal (CSM) in sow diets from less than 5% to 10% increased the incidence of premature farrowings (gestation length less than 111 days) from 1.1% to 2.7% (p less than 0.001) and reduced the mean gestation length from 114.07 +/- 1.53 to 113.70 +/- 1.59 days (p less than 0.0001). Survival of piglets born prematurely was poor. After removal of CSM from the diet there was a residual effect lasting several weeks before the gestation length returned to normal. Experimental feeding of diets containing 20% and 40% CSM to small groups of sows caused significant shortening of gestation length and 3 of 26 sows fed 40% CSM farrowed prematurely. The mechanism by which CSM causes this effect has yet to be determined.  相似文献   

Review of oral rehydration solutions for horses with diarrhoea     

P. ECKE  DR HODGSON  RJ ROSE 《Australian veterinary journal》1997,75(6):417-420

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Eimeria alabamensis and Eimeria brasiliensis in cattle in northern Queensland     

RJ PARKER 《Australian veterinary journal》1991,68(11):365-366

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