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Reconstructing past ocean pH-depth profiles 总被引：1，自引：0，他引：1
Measurement of boron isotope compositions in species of planktonic foraminifera that calcified their tests at different depths in the water column are used to reconstruct the pH profile of the upper water column of the tropical ocean. Results for five time windows from the middle Miocene to the late Pleistocene indicate pH-depth profiles similar to that of the modern ocean in this area, which suggests that this method may greatly aid in our understanding of the global carbon cycle. 相似文献
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ZF Ren ZP Huang JW Xu JH Wang P Bush MP Siegal PN Provencio 《Science (New York, N.Y.)》1998,282(5391):1105-1107
Free-standing aligned carbon nanotubes have previously been grown above 700 degreesC on mesoporous silica embedded with iron nanoparticles. Here, carbon nanotubes aligned over areas up to several square centimeters were grown on nickel-coated glass below 666 degreesC by plasma-enhanced hot filament chemical vapor deposition. Acetylene gas was used as the carbon source and ammonia gas was used as a catalyst and dilution gas. Nanotubes with controllable diameters from 20 to 400 nanometers and lengths from 0. 1 to 50 micrometers were obtained. Using this method, large panels of aligned carbon nanotubes can be made under conditions that are suitable for device fabrication. 相似文献
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Susceptibility of cats to equine morbillivirus 总被引：7，自引：0，他引：7
Objective To assess the susceptibility of cats to equine morbillivirus (EMV) by direct administration of the virus by subcutaneous, intra-nasal or oral routes, and following exposure to infected cats.
Design A disease transmission study, with controls, using ten cats.
Procedure Groups of cats were given the virus by the designated methods and assessed for evidence of infection by clinical examination, plus pathological and virological tests.
Results All cats administered the virus by subcutaneous, intra-nasal or oral routes became infected and developed the disease within 4 to 8 days. One of two cats in contact with affected cats also developed the disease, but two cats kept near to affected cats did not become infected. The virus was isolated from a range of tissues collected from the infected cats, and the lesions observed in affected cats were similar to those previously observed in horses naturally and experimentally infected with the virus.
Conclusion This is the first demonstration that animals can be infected with EMV by non-parenteral means, that the virus can transmit naturally between animals and confirms other reports of the similarity of EMV disease in horses and cats. 相似文献
Design A disease transmission study, with controls, using ten cats.
Procedure Groups of cats were given the virus by the designated methods and assessed for evidence of infection by clinical examination, plus pathological and virological tests.
Results All cats administered the virus by subcutaneous, intra-nasal or oral routes became infected and developed the disease within 4 to 8 days. One of two cats in contact with affected cats also developed the disease, but two cats kept near to affected cats did not become infected. The virus was isolated from a range of tissues collected from the infected cats, and the lesions observed in affected cats were similar to those previously observed in horses naturally and experimentally infected with the virus.
Conclusion This is the first demonstration that animals can be infected with EMV by non-parenteral means, that the virus can transmit naturally between animals and confirms other reports of the similarity of EMV disease in horses and cats. 相似文献
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Stable integration and expression of a cry1Ia gene conferring resistance to fall armyworm and boll weevil in cotton plants 
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Polymerase chain reaction and other laboratory techniques in the diagnosis of long incubation rabies in Australia 总被引：3，自引：0，他引：3
KA MCCOLL AR GOULD PW SELLECK PT HOOPER HA WESTBURY JS SMITH† 《Australian veterinary journal》1993,70(3):84-89
SUMMARY Blood and post-mortem tissues from a 10-years-old girl were submitted to the Australian Animal Health Laboratory. Clinical signs and histopathological lesions had suggested a diagnosis of rabies, but, an unusually long incubation period of at least 5 years did not encourage such a diagnosis. Serological examinations by the rapid fluorescent focus inhibition test revealed a dramatic increase in rabies virus-neutralising antibody during the 10-day period of hospitalisation. The results of a fluorescent antibody test on brain smears, and an immunoperoxidase test on formalin-fixed sections of brain were also consistent with a diagnosis of rabies. Attempts to isolate virus were unsuccessful. Polymerase chain reactions (PCRs) were conducted on a 10% suspension of a post-mortem sample from the patient's brain, using primers based on the published sequence of the Pasteur virus strain of rabies virus. 413 and 513 bp fragments from the nucleoprotein gene and a 403 bp fragment from the glycoprotein gene were amplified. Subsequent sequencing of these fragments, and comparison with equivalent regions of known rabies viruses, confirmed that the fragments originated from a virus belonging to the rabies virus serotype. This case demonstrated the advantage of using a range of laboratory techniques to obtain a definitive diagnosis. In particular, a PCR-based test may allow a diagnosis, even in the face of conditions that preclude virus isolation such as apparently occurred in this case. Finally, this case demonstrated that an unusually long incubation period should not discourage a tentative clinical diagnosis of rabies. 相似文献