Efficacy of Hyperimmunized Plasma in the Treatment of Horses with Acute Colitis     

Rachel P. Atherton  Martin O. Furr  Harold C. McKenzie  Anne M. Desrochers 《Journal of Equine Veterinary Science》2011,31(1):19-25

Colitis in the adult horse is a life-threatening clinical condition that can be caused by any of several enteric pathogens. This study was conducted to determine whether treating horses with plasma obtained from donors that were hyperimmunized against the common equine diarrheal pathogens Clostridium difficile, Clostridium perfringens, and Salmonella sp shortens the duration of diarrhea in acute colitis. To evaluate the efficacy of plasma treatment, 42 horses with acute onset of diarrhea were studied. Horses were enrolled if they were of age >1 year, duration of diarrhea at presentation was <72 hours, and they had not received equine plasma within the last 3 months. In addition, the serum cortisol concentrations of horses with acute diarrhea were studied.Horses were randomized to receive hyperimmunized plasma, control plasma (collected from nonimmunized horses), or no plasma therapy. Clinical parameters and fecal consistency were observed until resolution, discharge, or death, and complete blood counts (CBCs) and biochemical profiles were collected throughout the study. A total of 38 horses completed the study. The mean duration of diarrhea was 40.7 ± 9.8 (mean ± SEM) hours, 119.2 ± 56.1 hours, and 72.0 ± 24.5 hours for the hyperimmunized plasma, normal plasma (NP), and control groups, respectively. Using survival analysis techniques, this difference did not achieve statistical significance (P = .374). Serum cortisol was found to be increased in all horses at presentation and to decrease with time in all treatment groups. There was no difference in cortisol concentrations between the three treatment groups studied (P = .237).  相似文献   

Immunohistochemical and histomorphometric evaluation of vascular distribution in intact canine cranial cruciate ligament     

Hayashi K  Bhandal J  Kim SY  Rodriguez CO  Entwistle R  Naydan D  Kapatkin A  Stover SM 《Veterinary surgery : VS》2011,40(2):192-197

Objectives: To (1) describe vascular distribution in the grossly intact canine cranial cruciate ligament (CCL) using immunohistochemical techniques specific to 2 components of blood vessels (factor VIII for endothelial cells, laminin for basement membrane); and (2) compare the vascularity in different areas of interest (craniomedial versus caudolateral bands; core versus epiligamentous regions; and proximal versus middle versus distal portions) in the intact normal canine CCL. Study Design: In vitro study. Animals: Large, mature dogs (n=7) of breeds prone to CCL disease that were euthanatized for nonorthopedic conditions. Methods: Intact CCL were collected from fresh canine cadavers free from stifle pathology. CCL tissue was processed for immunohistochemistry and stained for factor VIII and laminin. Vascular density was determined by histomorphometric analysis. Results: Specific vascular staining was sparsely identified throughout the CCL; however, the proximal portion of the CCL appears to have a greater number of vessels than the middle or distal portion of the ligament. Conclusions: The CCL is a hypovascular tissue and its vascular distribution is not homogeneous.  相似文献   

Impact of acute conjunctivitis on ocular surface homeostasis in dogs     

Lionel Sebbag  Elizabeth A. Soler  Rachel A. Allbaugh  Jonathan P. Mochel 《Veterinary ophthalmology》2020,23(5):828-833

  相似文献   

Residual setup error in the canine intracranial region after megavoltage,kilovoltage, or cone‐beam computed tomographic image guidance for radiation therapy     

Celina Y. Morimoto  Monique N. Mayer  Narinder Sidhu  Rachel Bloomfield  Cheryl L. Waldner 《Veterinary and comparative oncology》2020,18(2):199-205

Sources of residual setup error after image guidance include image localization accuracy, errors associated with image registration, and inability of some treatment couches to correct submillimeter translational errors and/or pitch and roll errors. The purpose of this experimental study was to measure setup error after image‐guided correction of the canine intracranial region, using a four degrees‐of‐freedom couch capable of 1 mm translational moves. Six cadaver dogs were positioned 45 times as for clinical treatment using a vacuum deformable body cushion, a customizable head cushion, a thermoplastic mask and an indexed maxillary plate with a dental mould. The location of five fiducial markers in the skull bones was compared between the reference position and after megavoltage (MV), kilovoltage (kV) and cone‐beam computed tomography (CBCT)‐guided correction using orthogonal kV images. The mean three‐dimensional distance vectors (3DDV) after MV, kV and CBCT‐guided correction were 1.7, 1.5 and 2.2 mm, respectively. All values were significantly different (P < .01). The 95th percentiles of the 3DDV after online MV, kV and CBCT‐guided correction were 2.8, 2.6 and 3.6 mm, respectively. Residual setup error in the clinical scenario examined was on the order of millimetres and should be considered when choosing PTV margins for image‐guided radiation therapy of the canine intracranial region.  相似文献   

City sanitation and socioeconomics predict rat zoonotic infection across diverse neighbourhoods     

Maureen H. Murray  Mason Fidino  Rebecca Fyffe  Kaylee A. Byers  James B. Pettengill  Kerry S. Sondgeroth  Halcyon Killion  Seth B. Magle  Maria Jazmin Rios  Nora Ortinau  Rachel M. Santymire 《Zoonoses and public health》2020,67(6):673-683

Rat‐associated zoonoses transmitted through faeces or urine are of particular concern for public health because environmental exposure in homes and businesses may be frequent and undetected. To identify times and locations with greater public health risks from rats, we investigated whether rat characteristics, environmental features, socioeconomic factors, or season could predict rat infection risk across diverse urban neighbourhoods. In partnership with a pest management company, we sampled rats in 13 community areas along an income gradient in Chicago, a large city where concern about rats has increased in recent years. We collected kidneys for Leptospira spp. testing and colon contents for aerobic bacteria such as Salmonella spp. and Escherichia coli. Of 202 sampled rats, 5% carried Leptospira spp. and 22% carried E. coli. Rats were significantly more likely to carry Leptospira spp. on blocks with more standing water complaints in higher‐income neighbourhoods (OR = 6.74, 95% CI: 1.54–29.39). Rats were significantly more likely to carry E. coli on blocks with more food vendors (OR = 9.94, 2.27–43.50) particularly in low‐income neighbourhoods (OR = 0.26, 0.09–0.82) and in the spring (OR = 15.96, 2.90–88.62). We detected a high diversity of E. coli serovars but none contained major virulence factors. These associations between environmental features related to sanitation and infection risk in rats support transmission through water for Leptospira spp. and faecal–oral transmission for E. coli. We also found opposing relationships between zoonotic infection risk and income for these two pathogens. Thus, our results highlight the importance of sanitation for predicting zoonotic disease risks and including diverse urban areas in pathogen surveillance to mitigate public health risks from rats.  相似文献   

Outbreak of human infections with uncommon Salmonella serotypes linked to pet bearded dragons, 2012–2014     

Craig A. Kiebler  Lyndsay Bottichio  Latoya Simmons  Colin Basler  Rachel Klos  Nikos Gurfield  Elizabeth Roberts  Akiko Kimura  Linda S. Lewis  Kiyomi Bird  Felicia Stiles  Linda K. Schlater  Kristina Lantz  Thomas Edling  Casey Barton Behravesh 《Zoonoses and public health》2020,67(4):425-434

Reptiles are one of the fastest growing sectors in the United States pet industry. Reptile‐associated salmonellosis (RAS) continues to be an important public health problem, especially among children. We investigated an outbreak of human Salmonella infections resulting from serotypes Cotham and Kisarawe, predominately occurring among children. An outbreak of illnesses was identified in persons with exposure to pet bearded dragon lizards. Human and animal health officials, in cooperation with the pet industry, conducted epidemiologic, traceback and laboratory investigations. Onsite sampling was conducted at two US breeding facilities, one foreign breeding facility, and a large pet retail chain. A total of 166 patients in 36 states were identified with illness onset dates from 02/2012–06/2014. The median patient age was 3 years (range, <1–79 years), 57% were aged ≤5 years, and 37% were aged ≤1 year. Forty‐four patients (37%) were hospitalized, predominantly children. Sampling at breeding facilities and a national pet store chain resulted in isolation of outbreak serotypes at each facility; isolation proportions ranged from 2%–24% of samples collected at each facility.Epidemiologic, microbiologic and traceback evidence linked an outbreak of uncommon Salmonella serotypes to contact with pet bearded dragons. The high proportion of infants involved in this outbreak highlights the need to educate owners about the risk of RAS in children and the potential for household contamination by pet reptiles or their habitats. Strategies should be developed to improve breeding practices, biosecurity and monitoring protocols to reduce Salmonella in the pet reptile trade.  相似文献   

Effects of metronidazole on the fecal microbiome and metabolome in healthy dogs     

Rachel Pilla  Frederic P. Gaschen  James W. Barr  Erin Olson  Julia Honneffer  Blake C. Guard  Amanda B. Blake  Dean Villanueva  Mohammad R. Khattab  Mustafa K. AlShawaqfeh  Jonathan A. Lidbury  Jörg M. Steiner  Jan S. Suchodolski 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(5):1853-1866

  相似文献   

Genome-wide detection of signatures of selection in three Valdostana cattle populations     

Salvatore Mastrangelo  Slim Ben Jemaa  Elena Ciani  Gianluca Sottile  Angelo Moscarelli  Mekki Boussaha  Marina Montedoro  Fabio Pilla  Martino Cassandro 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(6):609-621

The Valdostana is a local dual purpose cattle breed developed in Italy. Three populations are recognized within this breed, based on coat colour, production level, morphology and temperament: Valdostana Red Pied (VPR), Valdostana Black Pied (VPN) and Valdostana Chestnut (VCA). Here, we investigated putative genomic regions under selection among these three populations using the Bovine 50K SNP array by combining three different statistical methods based either on allele frequencies (F_ST) or extended haplotype homozygosity (iHS and Rsb). In total, 8, 5 and 8 chromosomes harbouring 13, 13 and 16 genomic regions potentially under selection were identified by at least two approaches in VPR, VPN and VCA, respectively. Most of these candidate regions were population-specific but we found one common genomic region spanning 2.38 Mb on BTA06 which either overlaps or is located close to runs of homozygosity islands detected in the three populations. This region included inter alia two well-known genes: KDR, a well-established coat colour gene, and CLOCK, which plays a central role in positive regulation of inflammatory response and in the regulation of the mammalian circadian rhythm. The other candidate regions identified harboured genes associated mainly with milk and meat traits as well as genes involved in immune response/inflammation or associated with behavioural traits. This last category of genes was mainly identified in VCA, which is selected for fighting ability. Overall, our results provide, for the first time, a glimpse into regions of the genome targeted by selection in Valdostana cattle. Finally, this study illustrates the relevance of using multiple complementary approaches to identify genomic regions putatively under selection in livestock.  相似文献   

A comparison of thyroid hormone levels and plasma capillary zone electrophoresis in red-eared sliders (Trachemys scripta elegans) and map turtles (Graptemys spp.) depending on season and sex     

Christoph Leineweber  Sabine Öfner  Anke C. Stöhr  Rachel E. Marschang  Karina Mathes 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2020,49(1):78-90

  相似文献   

Risk assessment of Aphanomyces euteiches root rot disease: quantification of low inoculum densities in field soils using droplet digital PCR     

Gibert  Simon  Edel-Hermann  Véronique  Moussa Mcolo  Rayanti  Gautheron  Elodie  Michel  Joël  Bernaud  Eric  Gautheron  Nadine  Sol  Jean-Marie  Capelle  Gery  Galland  Rachel  Bardon-Debats  Arnaud  Lambert  Claudine  Steinberg  Christian 《European journal of plant pathology / European Foundation for Plant Pathology》2021,160(3):503-518

European Journal of Plant Pathology - Fusarium verticillioides is a soil-borne plant pathogen of maize plants (Zea mays L.) responsible for major yield losses by causing root, stalk, and ear rot...  相似文献