Malignant mesenchymoma with widespread metastasis including bone marrow involvement in a dog     

Kristen M. Weishaar  Elijah F. Edmondson  Douglas H. Thamm  Christine S. Olver 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(3):447-452

A male castrated Golden Retriever was presented for evaluation of a large mass over the left shoulder extending to the lower part of the neck that had been present for an extended period of time, but had a recent history of rapid growth. Previous aspirates of the mass were consistent with a lipoma. The mass was surgically excised and was diagnosed as an extraskeletal osteosarcoma based on histopathology. After surgery, the dog was initiated on a chemotherapy protocol with carboplatin and metronomic cyclophosphamide. He became neutropenic, anemic, and thrombocytopenic 14 days after the carboplatin treatment was administered. The neutropenia resolved, but the anemia and thrombocytopenia progressed. A bone marrow aspirate revealed erythroid hypoplasia, myeloid hyperplasia with a predominance of early precursors, and a subset of cells that made up 20% of the total population that were reported as bizarre and unclassifiable. These cells were discrete in nature and were thought to be hematopoietic in origin. The dog was euthanized due to deterioration of the clinical condition. On postmortem examination, widespread metastasis involving the lungs, liver, kidney, heart, and bone marrow was found. Histopathology of the tumor lesions determined 2 distinct malignant populations of liposarcoma and osteosarcoma, consistent with malignant mesenchymoma. However, the possibility of 2 separate neoplastic processes cannot be definitively excluded. This is the first report of bone marrow metastasis of a malignant mesenchymoma in a dog.  相似文献   

Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species     

Ramona Thieme  Ulrich Darsow  Tatyana Gavrilenko  Dimitriy Dorokhov  Horst Tiemann 《Euphytica》1997,97(2):189-200

Interspecific somatic hybrids between dihaploid breeding clones of potato, S. tuberosum and two accessions of wild Mexican species S. pinnatisectum and the hybrid line S. pinnatisectum × S. bulbocastanum were regenerated following electrofusion of mesophyll protoplasts to combine important agricultural traits of S. tuberosum and a high level of late blight resistance from selected wild accessions. In two fusion combinations 239 calli were regenerated; 162 from 195 calli analysed were identified as hybrids by means of isozyme analysis of peroxidases and, for some hybrid clones, by RAPD analysis. Depending on the fusion combination, 47–89 percent of the somatic hybrids had the expected ploidy level and 7–16 percent were mixoploids. Somatic hybrids were phenotypically intermediate as compared to their parents and some of them were able to be backcrossed sexually with potato. Fertility and crossability depended on combination and ploidy level of the somatic hybrids. In tests with detached leaves the wild partner clones had a high late blight resistance score of 8,6 and 8,9; the susceptible tuberosum-partners of 2,8 and 3,5, respectively. Nearly 25 percent of somatic hybrids had a resistance level of 6 or higher in the first year of assessment. The average resistance value of most somatic hybrids was lower than the average parental level. The reasons for variation in resistance values are discussed in connection with the practical application of fusion hybrids. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Ecological level analysis of primary lung tumors in dogs and cats and environmental radon activity     

Brittany L. Fowler  Chad M. Johannes  Annette O'Connor  Deanna Collins  Jonathan Lustgarten  Chaohui Yuan  Kristen Weishaar  Kelly Sullivan  Kelly R. Hume  Jennifer Mahoney  Brittany Vale  Alicia Schubert  Valerie Ball  Katie Cooley-Lock  Kaitlin M. Curran  Laura Nafe  Allison Gedney  Megan Weatherford  Dana N. LeVine 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(6):2660-2670

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Phosphorylated KIT as a predictor of outcome in canine mast cell tumours treated with toceranib phosphate or vinblastine     

Douglas H. Thamm  Kristen M. Weishaar  Joseph B. Charles  E. J. Ehrhart 《Veterinary and comparative oncology》2020,18(2):169-175

Canine cutaneous mast cell tumour (MCT) is the most common malignant skin tumour in dogs and can exhibit variable biologic behaviour. Dysregulated signalling through the receptor tyrosine kinase (RTK) KIT can promote cell proliferation and survival, and assessment of its dysregulation via detection of activating c‐kit gene mutations or assessment of KIT protein localization is associated with multiple features of malignancy. The aim of the current study was to use a previously validated immunohistochemical (IHC) assay to directly measure phosphorylated KIT (pKIT) in order to investigate its association with other established prognostic markers, response to therapy, progression free interval (PFI) and overall survival time (OST) in dogs treated medically for measurable MCT. Tumour tissue from 74 dogs enrolled in a prospective study comparing toceranib and vinblastine for MCT treatment were evaluated for pKIT immunoreactivity. pKIT was variably expressed, with some degree of positivity observed in 49/74 cases (66%). pKIT immunoreactivity was significantly associated with aberrant KIT localization, high mitotic index and high histologic grade. On univariate analysis, pKIT immunoreactivity predicted shorter PFI and OST in the entire patient population as well as shorter PFI in the toceranib treated group, and was the sole predictive factor for OST upon multivariate analysis, while mitotic index was the sole independent predictive factor for PFI. These results demonstrate that IHC detection of pKIT correlates with several features of aggressive behaviour, and may confer information that is complementary to other prognostic factors. However, the role of pKIT in predicting outcome needs to be studied further before recommendations can be made for its routine use.  相似文献   

Associations between self-reported health conscious consumerism, body-mass index, and attitudes about sustainably produced foods     

Ramona Robinson  Chery Smith 《Agriculture and Human Values》2003,20(2):177-187

An evaluation was made of theassociations between self-reported healthconscious consumerism, body-mass index (BMI),and consumer beliefs, attitudes, intentions,and behaviors regarding sustainably producedfoods. Self-administered surveys were completedby adult consumers (n = 550) in threemetropolitan Minnesota grocery stores. Selecteddemographic and psychographic differencesbetween health conscious consumers andnon-health conscious consumers were evaluated.Compared to non-health conscious consumers,health conscious consumers were more likely tobe female, older, more educated, higher incomeearners, more active, healthier, and possess ahealthier body mass index. They also held moresupportive beliefs, attitudes, and intentionswith regard to sustainably produced foods. Inconclusion, some consumers are interested insupporting sustainable production practices andtheir support may be linked to improvedpersonal, environmental, and communityhealth.  相似文献   

Economic growth and nutrition transition:an empirical analysis comparing demand elasticities for foods in China and Russia     

Christine Burggraf  Lena Kuhn  ZHAO Qi-ran  Ramona Teuber  Thomas Glauben 《中国农业科学(英文版)》2015,(6)

This study provides empirical evidence on the link between economic growth and nutrition transition in two emerging econ-omies, China and Russia. Both countries have experienced rising average incomes,...  相似文献   

Phytoremediation of a multi contaminated soil: mercury and arsenic phytoextraction assisted by mobilizing agent and plant growth promoting bacteria     

Elisabetta Franchi  Eleonora Rolli  Ramona Marasco  Gloria Agazzi  Sara Borin  Paola Cosmina  Francesca Pedron  Irene Rosellini  Meri Barbafieri  Gianniantonio Petruzzelli 《Journal of Soils and Sediments》2017,17(5):1224-1236

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Sex-related variations in bone microstructure of rabbits intramuscularly exposed to patulin     

Hana Duranova  Veronika Kovacova  Ramona Babosova  Radoslav Omelka  Maria Adamkovicova  Birgit Grosskopf  Marcela Capcarova  Monika Martiniakova 《Acta veterinaria Scandinavica》2015,57(1)

Background

Patulin, a toxic mold metabolite, has been found as natural contaminant of processed fruits, most notably apples, apple juices and other apple-based products. A number of adverse health effects in humans and animals are associated with patulin intoxication. The current study was performed to analyse possible toxic effects of patulin on femoral bone microstructure in adult rabbits in detail. Fourteen clinically healthy four-month-old rabbits of both sexes (6 males and 8 females) were included in the study. Animals from the experimental groups (group E♂, n = 3; group E♀, n = 4) were injected intramuscularly with patulin at dose 10 μg/kg body weight two times a week for 28 days. The dose of patulin was estimated based on the maximum permitted level of patulin for apple products intended for infants and young children. Three males and four females without patulin administration served as controls (groups C♂ and C♀). Cortical bone thickness and qualitative and quantitative histological characteristics of compact bone tissue were investigated.

Results

Intramuscular applications of patulin significantly increased the thickness of cortical bone in both sexes of rabbits. In patulin-exposed males, an absence of primary vascular longitudinal bone tissue near the endosteal border was observed, which could be associated with intensive bone remodeling. Femoral diaphyses of females displayed a lower number of secondary osteons in the middle part of the substantia compacta, and occurrence of the osteons near the periosteum. This could indicate alterations in bone turnover. Histomorphometrical evaluations showed significantly increased sizes of the primary osteons’ vascular canals (P < 0.05) in males exposed to patulin possibly due to mycotoxin-induced increased levels of testosterone.

Conclusions

This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits. Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent.  相似文献   

Vinorelbine rescue therapy for dogs with primary urinary bladder carcinoma          下载免费PDF全文

M. E. Kaye  D. H. Thamm  K. Weishaar  J. A. Lawrence 《Veterinary and comparative oncology》2015,13(4):443-451

The goal of this study was to evaluate the anti‐tumour activity and toxicoses of vinorelbine as a palliative rescue therapy for dogs with primary urinary bladder carcinoma. Thirteen dogs refractory to prior chemotherapeutics and one dog naïve to chemotherapeutic treatment were enrolled. Vinorelbine (15 mg m^?2 IV) was administered intravenously along with concurrent oral anti‐inflammatory drugs, if tolerated. A median of six doses of vinorelbine (range: 1–16) was administered. Two dogs (14%) had partial responses, and eight (57%) experienced stable disease. Subjective improvement in clinical signs was noted in 11 dogs (78%). Adverse events were mild and primarily haematological in nature. Median time to progression was 93 days (range: 20–239 days). Median survival time for all dogs was 187 days; median survival for 13 pre‐treated dogs was 207 days. Vinorelbine may have utility in the management of canine primary urinary bladder carcinoma and should be evaluated in a prospective study.  相似文献   

Mapping of a novel,major late blight resistance locus in the diploid (1EBN) Mexican Solanum pinnatisectum Dunal on chromosome VII          下载免费PDF全文

Marion Nachtigall  Janine König  Ramona Thieme 《Plant Breeding》2018,137(3):433-442

Late blight caused by the oomycete Phytophthora infestans (Mont.) de Bary (Pi) is the most important foliar disease of potato worldwide. An intraspecific hybrid between individuals of a resistant and a susceptible S. pinnatisectum accession was backcrossed to the susceptible parent to generate a segregating population for late blight resistance consisting of 84 plants. In detached‐leaflet assays, reaction to late blight segregated in a 1r:1s manner in BC₁ progeny indicating the presence of a single dominant resistance gene. A genetic map was constructed based on 1,583 DArT/SSR markers which were allocated to 12 linkage groups, covering 1,793.5 cM with an average marker distance of 1.1 cM. The late blight resistance locus derived from S. pinnatisectum was mapped on chromosome VII. In comparison with the previously reported resistance genes Rpi1 and Rpi2, the new target resistance locus most likely is located on the opposite arm of chromosome VII. Results of this study will serve as a basis for future fine mapping of the late blight resistance locus and the development of locus‐specific markers for marker‐assisted selection.  相似文献