Effects of photoperiod on salsolinol‐induced prolactin secretion in goats     

Tomoyoshi YAEGASHI  Jin JIN  Tatsuru SAWADA  Hayato SAITO  Ferenc FÜLÖP  György Miklos NAGY  Tsutomu HASHIZUME 《Animal Science Journal》2012,83(5):418-425

The aim of the present study was to clarify the relation between salsolinol (SAL)‐induced prolactin (PRL) release and photoperiod in goats. A single intravenous (i.v.) injection of SAL was given to adult female goats under short (8 h light, 16 h dark) or long (16 h light, 8 h dark) photoperiod conditions at two different ambient temperatures (20°C or 5°C), and the PRL‐releasing response to SAL was compared to that of thyrotropin‐releasing hormone (TRH) or a dopamine (DA) receptor antagonist, sulpiride. SAL, as well as TRH or sulpiride, stimulated the release of PRL promptly after each injection in both 8‐ and 16‐h daily photoperiods at 20°C (P < 0.05). The area under the response curve (AUC) of PRL for the 60‐min period after injections of saline (controls), SAL, TRH and sulpiride in the 16‐h daily photoperiod group was greater than each corresponding value in the 8‐h daily photoperiod group (P < 0.05). There were no significant differences in the AUC of PRL among the values produced after the injection of SAL, TRH and sulpiride in 16‐h daily photoperiod group; however, the values produced after the injection of TRH were smallest among the three in the 8‐h daily photoperiod group (P < 0.05). The PRL‐releasing responses to SAL, TRH and sulpiride under a short and long photoperiod condition at 5°C resembled those at 20°C. These results show that a long photoperiod highly enhances the PRL‐releasing response to SAL as well as TRH or sulpiride in either medium or low ambient temperature in goats.  相似文献   

Effect of Supplemental Vitamin E on Antibody Titer in Japanese Black Calves Vaccinated against Bovine Herpesvirus-1     

Konosuke OTOMARU  Shun SAITO  Karura ENDO  Masayuki KOHIRUIMAKI  Shin-ichi FUKUYAMA  Hiromichi OHTSUKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1671-1673

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Identification of a potent immunostimulatory oligodeoxynucleotide from Streptococcus thermophilus lacZ     

Takeshi SHIMOSATO  Masanori TOHNO  Takashi SATO  Junko NISHIMURA  Yasushi KAWAI  Tadao SAITO  Haruki KITAZAWA 《Animal Science Journal》2009,80(5):597-604

Immunostimulatory sequences of oligodeoxynucleotides (ODNs), such as CpG ODNs, are potent stimulators of innate immunity. Here, we identified a strong immunostimulatory CpG ODN, which we named MsST, from the lac Z gene of Streptococcus (S.) thermophilus ATCC19258, and we evaluated its immune functions. In in vitro studies, MsST had a similar ability as the murine prototype CpG ODN 1555 to induce inflammatory cytokine production and cell proliferation. In mouse splenocytes, MsST increased the number of CD80+CD11c+and CD86+CD11c+ dendritic cells and CD4+CD25+ regulatory T cells. We also analyzed the effects of MsST on the expression of regulatory cytokines by real-time quantitative PCR. MsST was more potent at inducing interleukin-10 expression than the ODN control 1612, indicating that MsST can augment the regulatory T cell response via Toll-like receptor 9, which plays an important role in suppressing T helper type 2 responses. These results suggest that S. thermophilus , whose genes include a strong Immunostimulatory sequence-ODN, is a good candidate for a starter culture to develop new physiologically functional foods and feeds.  相似文献   

Microbiota analysis of Caspian Sea yogurt, a ropy fermented milk circulated in Japan     

Kenji UCHIDA  Keiko AKASHI  Hidemasa MOTOSHIMA  Tadasu URASHIMA  Ikichi ARAI  Tadao SAITO 《Animal Science Journal》2009,80(2):187-192

We analyzed the microbiota of domestic ropy fermented milk, Caspian Sea yogurt (or 'kasupikai yohguruto' in Japanese), circulated in Japan. We collected six varieties from five localities. Lactococcus ( L) lactis ssp. cremoris was isolated from all samples as the dominant strain at levels of 10⁸−10⁹ CFU/g. We show this strain produces an extracellular polysaccharide (EPS) that causes the unique characteristic viscosity of the product. From analysis of the RAPD pattern of 60 bacterial isolates from the six samples, we found that 59 strains from a total of 60 isolates were identical and produced this viscosity. Furthermore, PFGE analysis of representative strains from each sample indicated that the isolates could be classified into four subgroups. This suggests these L. lactis ssp. cremoris strains found in Caspian Sea yogurt may have been slightly mutated during subculture in Japan. In addition, Lactobacillus (L.) sakei ssp. sakei were isolated from three samples; L. plantarum , Gluconoacetobacter sacchari and Acetobacter aceti were isolated from two samples; and L. paracasei , L. kefiri , Leuconostoc ( Leu. ) mesenteroides were isolated from one sample.  相似文献   

Steady-state pharmacokinetics of zonisamide in plasma, whole blood, and erythrocytes in dogs     

K. FUKUNAGA  M. SAITO  M. MUTO  R. YOSHIOKA  K. MISHIMA  M. FUJIWARA  & K. ORITO 《Journal of veterinary pharmacology and therapeutics》2010,33(1):103-106

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中国和日本农业活动对流域氮素收支的影响     

S. D. KIMURA  YAN Xiao-Yuan  R. HATANO  A. HAYAKAWA  K. KOHYAMA  TI Chao-Pu  DENG Mei-Hu  M. HOJITO  S. ITAHASHI  K. KURAMOCHI  CAI Zu-Cong  M. SAITO 《土壤圈》2012,22(2):137-151

To analyze the effect of agricultural activity on nitrogen(N) budget at the watershed scale,a comparative study was conducted at two Japanese watersheds,the Shibetsu River watershed(SRW) and Upper-Naka River watershed(UNRW),and one Chinese watershed,the Jurong Reservoir watershed(JRW).The total area and the proportion of agricultural area(in parentheses) of the watersheds were 685(51%),1 299(21%),and 46 km 2(55%) for SRW,UNRW,and JRW,respectively.The main agricultural land use in SRW was forage grassland,while paddy fields occupied the highest proportion of cropland in UNRW(11% of total area) and JRW(31% of total area).The farmland surplus N was 61,48,and 205 kg N ha 1 year 1 for SRW,UNRW,and JRW,respectively.The total input and output for the whole watershed were 89 and 76,83 and 61,and 353 and 176 kg N ha 1 year 1 for SRW,UNRW,and JRW,respectively.The proportion of discharged N to net anthropogenic N input was 31%,37%,and 1.7% for SRW,UNRW,and JRW,respectively.The two watersheds in Japan showed similar proportions of discharged N to those of previous reports,while the watershed in China(JRW) showed a totally different characteristic compared to previous studies.The high N input in JRW did not increase the amount of discharged N at the outlet of the watershed due to high proportions of paddy fields and water bodies,which was an underestimated N sink at the landscape scale.  相似文献   

Water re-use system for smolt production     

Svein Risa  Harald Skjervold 《Aquaculture (Amsterdam, Netherlands)》1975,6(2):191-195

A recirculation unit for salmon smolt production is described. The preliminary results for growth rate and viability of the fry were satisfactory.  相似文献   

Identification of domestic cat hepadnavirus from a cat blood sample in Japan     

Kazuki TAKAHASHI  Yasuyuki KANEKO  Akiko SHIBANAI  Shushi YAMAMOTO  Ayana KATAGIRI  Tatsuyuki OSUGA  Yoshiyuki INOUE  Kohei KURODA  Mika TANABE  Tamaki OKABAYASHI  Kiyokazu NAGANOBU  Isao MINOBE  Akatsuki SAITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):648

The hepatitis B virus (Hepadnaviridae) induces chronic hepatitis and hepatic cancer in humans. A novel domestic cat hepadnavirus (DCH) was recently identified in several countries, however, the DCH infection status of cats in Japan is unknown. Therefore, we investigated the DCH infection rate of 139 cat samples collected in Japan. We identified one positive blood sample (0.78%) from a 17-year-old female cat with chronically elevated alanine aminotransferase. Phylogenetic analysis demonstrated that the DCH strain identified in this study is genetically different from strains in other countries. Further investigations are required to elucidate the evolution of DCH and the impact of DCH infection on hepatic diseases in domestic cats.  相似文献   

Kazakhstani native cattle reveal highly divergent mtDNA from Bos taurus and Bos indicus lineages with an absence of Bos indicus Y chromosome     

Hayate Yamanaka  Kako Murata  Risa Tabata  Fuki Kawaguchi  Shinji Sasazaki  Yoshio Yamamoto  Meirat Bakhtin  Polat Kazymbet  Alykhan Meldevekob  Maratbek Z. Suleimenov  Masahide Nishibori  Hideyuki Mannen 《Animal Science Journal》2019,90(1):29-34

Kazakhstan is the largest landlocked country and contains two important propagation routes for livestock from the Fertile Crescent to Asia. Therefore, genetic information about Kazakhstani cattle can be important for understanding the propagation history and the genetic admixture in Central Asian cattle. In the present study, we analyzed the complete mtDNA D‐loop sequence and SRY gene polymorphism in 122 Kazakhstani native cattle. The D‐loop sequences revealed 79 mitochondrial haplotypes, with the major haplogroups T and I. The Bos taurus subhaplogroups consisted of T (3.3%), T1 (2.5%), T2 (2.5%), and T4 (0.8%) in addition to the predominant subhaplogroup T3 (86.9%), and the Bos indicus subhaplogroup of I1 (4.1%). Subsequently, we investigated the paternal lineages of Bos taurus and Bos indicus, however, all Kazakhstani cattle were shown to have Y chromosome of Bos taurus origin. While highly divergent mtDNA subhaplogroups in Kazakhstani cattle could be due to the geographical proximity of Kazakhstan with the domestication center of the Fertile Crescent, the absence of Bos indicus Y chromosomes could be explained by a decoupling of the introgression dynamics of maternal and paternal lineages. This genetic information would contribute to understanding the genetic diversity and propagation history of cattle in Central Asia.  相似文献   

The Eurasian Steppe is an important goat propagation route: A phylogeographic analysis using mitochondrial DNA and Y‐chromosome sequences of Kazakhstani goats     

Risa Tabata  Fuki Kawaguchi  Shinji Sasazaki  Yoshio Yamamoto  Meirat Bakhtin  Polat Kazymbet  Alykhan Meldevekob  Maratbek Z. Suleimenov  Masahide Nishibori  Hideyuki Mannen 《Animal Science Journal》2019,90(3):317-322

Goats (Capra hircus) were domesticated in the Fertile Crescent and propagated all over the world. The Silk Road through the Eurasian Steppe belt is a possible propagation route for domestic goats to Central Asia. Kazakhstan is in close geographical proximity to domestication centers and covers the majority of the Eurasian Steppe belt. In this study, we examined the genetic diversity and phylogeographic structure of Kazakhstani goats. The mtDNA sequences of 141 Kazakhstani goats were categorized into haplogroups A, C, and D, of which haplogroup A was predominant (97%), whereas haplogroups C and D were detected at low frequencies (1.4% each). The Kazakhstani haplotypes C were thzen categorized into Asian mtDNA type. Sequence analysis of the SRY gene on the Y‐chromosome in 67 male Kazakhstani goats revealed two haplotypes: Y1A (64%) and Y2A (36%). Analysis of the distribution of mtDNA haplogroups and SRY haplotypes from Eurasia and Africa demonstrated genetic similarity among animals from Kazakhstan, Mongolia, and Northwest China located on the Eurasian Steppe belt. These phylogeographic results suggested that the Eurasian Steppe belt was an important propagation route for goats to Central Asia.  相似文献