牛子宫复旧过程中胶原蛋白和肌浆球蛋白降解规律的研究     

田文儒 Noak.  DE 《黑龙江畜牧兽医》1991,(6):10-12

本实验监测8头围产期奶牛外周血浆中胶原蛋白和肌浆球蛋白的代谢产物——羟基脯氨酸(HP)和3甲基组氨酸(3MH)的含量,借以找出这两种蛋白质的代谢规律和子宫复旧的关系。结果表明:荷兰黑白花牛和娟珊牛血浆中HP的含量和子宫角直径分别呈显著及极显著正相关(r=0.41,P<0.05和r=0.57 P<0.01),而3MH分别呈极显著和显著正相关(r=0.52 P<0.01和r=0.39(P<0.05)。此外,黑白花牛血浆中3MH的含量极显著高于娟珊牛(P<0.001)。两个品种牛子宫复旧的平均时间,黑白花牛为37.4±3.1天,娟珊牛32.3±2.1天。结果说明牛产后子宫体积的缩小与子宫肌肉的胶原蛋白和肌浆球蛋白降解成氨基酸排出体外有关,子宫复旧的速度可能决定于这两种蛋白质的降解速度。  相似文献   

Serological evidence for the presence of Leptospira interrogans serovar bratislava in Australian pigs     

RJ RICHAPPEL  WA ELLIS†  B ADLER‡  L AMON  BD MILLAR§  SS ZHU  RW PRIM§ 《Australian veterinary journal》1992,69(5):119-120

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Molecular basis of globoid cell leukodystrophy in Irish setters     

McGraw RA  Carmichael KP 《Veterinary journal (London, England : 1997)》2006,171(2):370-372

Globoid cell leukodystrophy (GLD), or Krabbe's disease, is a progressive autosomal recessive disorder of the central nervous system in man and in various other species. GLD has been shown to result from various mutations in the gene encoding galactocerebrosidase (GALC), a lysosomal enzyme. We investigated the molecular basis of GLD in a related group of Irish setters. Sequencing of the GALC cDNA from an affected individual revealed an insertion mutation of 78 base pairs (bp) consisting of 16 bp of insertion site duplication and 62 bp of sequence derived from the U4 small nuclear RNA. We implemented a PCR-based test which is useful for identifying carriers of the mutation.  相似文献   

An epidemiological study of Fasciola hepatica in the Netherlands     

C.P.H. Gaasenbeek  H.J. Over  N. Noorman  WA. de Leeuw 《The Veterinary quarterly》2013,33(4):140-144

Summary

Transmission of F.hepatica under natural conditions was analysed in a three year programme. The variables used were the indirect haemagglutination (IHA) technique, worm establishment in tracer lambs and the population dynamics, infection rate and shedding pattern of Lymnaea truncatula.

It is concluded that fluke eggs, infected snails and metacercariae on herbage can survive the winter in the Netherlands. Metacercarial availability was positively correlated to the amount of rainfall in the grazing period. The role of developed eggs that survive the winter is important, because this results in earlier infections in the herd.

The use of the serological diagnosis method IHA is important to detect F. hepatica infection in an early stage. Use of cellophane paper on floats is a useful method for determining the shedding pattern of cercariae from L. truncatula. It is concluded that collection of metacercariae on cellophane floats, inventarization of L. truncatula and its infection level are useful tools for the prediction of liverfluke infections.  相似文献   

Early Pregnancy Diagnosis by Serum Progesterone and Ultrasound in Sheep Carrying Somatic Cell Nuclear Transfer-Derived Pregnancies     

B Alexander  G Coppola  GF Mastromonaco  E St. John  ER Reyes  DH Betts  WA King 《Reproduction in domestic animals》2008,43(2):207-211

Early pregnancy diagnosis and monitoring play an important role following embryo transfer in sheep. The aims of the current study were to investigate (i) the pattern of serum progesterone profiles in sheep carrying somatic cell nuclear transfer (SCNT)‐derived (clone) pregnancies, and (ii) the frequency of pregnancy loss during development following SCNT embryo transfer. Sheep SCNT embryos were made using standard nuclear transfer techniques. Day 7 embryos were surgically transferred to oestrus‐synchronized recipients (n = 27). As a control, normal fertile ewes (n = 12) were bred by natural breeding. Serum was collected from all the ewes on the day of estrus (day 0 sample), 7 days post‐estrus (day 7 sample) and 19 days post‐estrus (day 19 sample) and every 10 days thereafter until lambing or pregnancy loss occurred. Serum progesterone (P4) was assessed using enzyme immunoassay. Pregnancy was confirmed by ultrasound scanning on day 35 of pregnancy followed by subsequent scanning every 10 days. In control ewes, pregnancy rate on day 35 was 83.3% (10/12), whereas in the ewes that received SCNT embryos, it was 22.2% (6/27; p < 0.05). The day 45 pregnancy rate in the control ewes was 83.3%, whereas in the SCNT embryo recipients it was 11.0% (p < 0.05). Hormone analysis revealed that SCNT embryo recipients exhibited a significantly lower P4 profiles at different time points in pregnancy compared to controls (p < 0.05). This study highlights the use of serum progesterone in combination with ultrasound for the investigation of embryo loss and crucial times during development of normal and SCNT embryos in sheep. Further, the serum P4 levels directly reflect the degree of placental development in these two groups.  相似文献   

Effect of a mindfulness exercise on stress in veterinary students performing surgery     

Brenda S. Stevens  Kenneth D. Royal  Kelli Ferris  Abigail Taylor  Amy M. Snyder 《Veterinary surgery : VS》2019,48(3):360-366

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Suppression of volcanism during rapid extension in the basin and range province, united states     

PB Gans  WA Bohrson 《Science (New York, N.Y.)》1998,279(5347):66-68

Continental extension and volcanism are generally thought to be complementary. Stratigraphic and structural data from some highly extended parts of the Basin and Range province reveal instead that rapid extension appears to have suppressed volcanism. This relation may reflect enhanced crystallization of midcrustal magmas during extension resulting from exsolution of magmatic volatiles, increased interaction of magmas with meteoric water, and dispersal of magma into smaller bodies. Some rift environments may thus be characterized by voluminous synextensional plutonism with little or no concomitant volcanism.  相似文献   

周小舟教授治疗肝癌经验          下载免费PDF全文

韩志毅  孙新峰  马文峰  张卫  周小舟 《勤云标准版测试》2016,36(9):57-59

周小舟教授认为肝癌的病因是内因正气亏虚和外因外染疫毒相互作用所致,其主要病机是气虚血瘀,针对主要病机设立芪术抗癌方辨证加减治疗,临床疗效显著;周教授通过实验及临床研究亦初步阐明了芪术抗癌方的作用机制,值得临床借鉴。  相似文献   

热处理对水牛乳稳定特性的影响     

瓦云超  赵海晴  李仁芳  刘演景  伍云  谢秉锵  蒋欣荣  瞿恒贤  王琴  张奇波  于凡  任远庆  顾瑞霞 《乳业科学与技术》2016,39(3):25-28

为比较不同热处理条件对水牛乳稳定性的影响,实验采用不同条件热处理后的pH缓冲容量、总溶解固体（total dissolved solids,TDS）、蛋白沉淀量及表观黏度等指标研究杂交高代水牛乳样品。结果表明：与未经处理的水牛乳样品相比,水牛乳经不同条件热处理后,其pH缓冲容量、TDS、蛋白沉淀量及表观黏度皆发生显著变化。其中水牛乳分别在经121℃/15 min和95℃/5 min处理后,其pH缓冲容量、TDS、蛋白沉淀量及表观黏度分别达到最大值和最小值。因此,认为在本实验研究范围内,水牛乳经121℃/15 min及95℃/5 min处理后,对其稳定性影响最大。  相似文献   

用于狂犬病常规诊断的一种组织培养感染试验     

Webs.  WA 《中国兽药杂志》1989,(2):45-48

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