Squamous cell carcinoma in a digit of the hind limb with systemic metastasis in a 17-year-old female koala     

Mio KOBAYASHI  Toshinori YOSHIDA  Risako YAMASHITA  Ryo ICHIKAWA  Junta NAKAHARA  Kazuki NAKAMURA  Hiromu OKANO  Yasunori TAKAHASHI  Nanao ITO  Makoto SHIBUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):994

We encountered a case of cutaneous squamous cell carcinoma (SCC) in a 17-year-old female koala at a zoo. A fragile, papillary, elevated mass was found on the third digit of the right hind limb. SCC was identified histopathologically: squamous cell-like polygonal tumor cells showed a nest-like growth pattern with epidermal down growth, central keratinization and necrotic foci, and invaded dermal connective tissues. Metastatic lesions were observed in various organs, including the lung and axillary lymph node: in the lung, multiple metastatic foci similar to the primary lesion, and in the axillary lymph node, individual polygonal tumor cells infiltrated the sinusoids. Immunohistochemistry revealed that the tumor cells were positive for proliferating cell nuclear antigen, which exhibited 32–33% of labeling indices in the tumor cells. To our knowledge, this is the first report of a case of SCC in a digit of a koala.  相似文献   

Seasonality of serum levels of vitellogenin in male Japanese whiting, Sillago japonica, reared under natural temperature and photoperiod     

Komei  HOTTA  Takayuki  WATANABE  Chiho  KISHIDA  Yukio  NAKAMURA  Nobuyuki  OHKUBO  Takahiro  MATSUBARA  Shinji  ADACHI  Kohei  YAMAUCHI 《Fisheries Science》2003,69(3):555-562

ABSTRACT:   Because blood vitellogenin (Vg) has been considered a biomarker for environmental estrogens, the basal levels of Vg and 17β-estradiol (E₂) were determined in male Japanese whiting reared under natural conditions. Serum levels of Vg and E₂ were measured and gonadal development was assessed by gonadosomatic index (GSI) and histological observation in 8–10 male fish at monthly intervals throughout the annual reproductive cycle. Serum E₂ was <60 pg/mL throughout the study period. In contrast, serum Vg exhibited seasonal changes: serum levels of Vg gradually increased from April to May (mean 63 ± 13 ng/mL and 124 ± 48 ng/mL in April and May, respectively), and then reached a peak value (mean 352 ± 68 ng/mL) in June. Thereafter, serum Vg gradually decreased, reaching undetectable levels (<50 ng/mL) in October. Serum levels of Vg tended to increase in the male fish in which the GSI was >1%. Histological observation revealed that testes in such male fish were in active spermatogenesis and then all of the testes of male fish in which serum Vg decreased to ND levels were regressed. These results suggest that Vg productive potency (sensitivity to estrogens) may increase in the spermatogenic stage, resulting in production of Vg in response to very low levels of natural or xenobiotic estrogens.  相似文献   

Two forms of cytochrome P450 cDNA from 3-methylcholanthrene-treated European eel Anguilla anguilla     

Shyamal C  MAHATA  Ryoichi  MITSUO  Jun-Ya  AOKI  Hironori  KATO  Takao  ITAKURA 《Fisheries Science》2003,69(3):615-624

ABSTRACT:     The cytochrome P450 (CYP) represents a large group of microsomal monooxygenases that catalyze drugs as well as a host of lethal environmental contaminants such as dioxins, leading to either detoxification and excretion from the animal or generation of carcinogenic intermediates. In the present study two forms of cDNA were cloned (Eu MC1 and Eu MC2) for European eel CYP1A genes by polymerase chain reaction (PCR) techniques. The cDNA of Eu MC1 was 3368 bp long coding 521 amino acid residues, and that of Eu MC2 was 2464 bp long coding 517 amino acid residues. Identities of deduced amino acid sequences between Eu MC1 and Japanese eel CYP1A1 and that between Eu MC2 and the second form of Japanese eel CYP1A were 98% and 97%, respectively, showing decisively that Eu MC1 and Eu MC2 are orthologous to Japanese eel CYP1A1 and the second form of CYP1A, respectively. A striking difference between the two eel species was that the Eu MC1 peptide was two amino acid residues longer than that of the Japanese eel CYP1A1. Existence of two loci of CYP1A in Japanese and European eels may suggest that the two forms of CYP1A exist widely among the eel species, because the divergence between the two eel species has been shown to be close to the basal divergence among eels. The identities in CYP1A may help to estimate genetic distance between European and Japanese eels.  相似文献   

Comparison of consumption of bluegill by Far Eastern catfish and largemouth bass     

Osamu  KATANO  Tomoyuki  NAKAMURA  Shoichiro  YAMAMOTO 《Fisheries Science》2003,69(5):989-994

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The development of angiotensin I-converting enzyme inhibitor derived from chicken bone protein   总被引：1，自引：1，他引：0  

Fu-Yuan CHENG  Yu-Tse LIU  Tien-Chun WAN  Liang-Chuan LIN  Ryoichi SAKATA 《Animal Science Journal》2008,79(1):122-128

In order to produce angiotensin I‐converting enzyme (ACE) inhibitor for application in functional food, chicken bones were gathered from a meat processing factory and then hydrolyzed with Alcalase, pepsin and trypsin for 12 h. The hydrolysates were lyophilized, stored at ?80°C and tested experimentally every 2 h for pH value, peptide content, degree of hydrolysis (DH), electrophoresis and activity of ACE inhibitor. The hydrolysates of Alcalase had the highest peptide content and DH. The components of more than 66 kDa had disappeared in hydrolysates of Alcalase and trypsin after 2 h of hydrolysis. The hydrolysates of Alcalase were more active in inhibiting ACE, especially when hydrolyzed at 4 and 8 h, and also had low IC50 values of 1.960 and 0.945 mg/mL. According to the results of DH and electrophoresis, the higher activity of ACE inhibitor is assumed to be derived from the low molecular peptides in hydrolysates of Alcalase. Chicken leg bone has a high potential to be utilized to develop ACE inhibitory peptides as a potential ingredient of functional food intended to alleviate hypertension.  相似文献   

The in vitro antioxidant properties of alcalase hydrolysate prepared from silkie fowl (Gallus gallus) blood protein          下载免费PDF全文

Fu‐Yuan Cheng  I‐Chun Lai  Liang‐Chuan Lin  Ryoichi Sakata 《Animal Science Journal》2016,87(7):921-928

Two types of proteins including blood plasma protein and blood cell protein were isolated from silkie fowl (Gallus gallus) blood and hydrolyzed using alcalase for 0, 2, 4 and 6 h. The blood plasma protein hydrolysate (BPH) and blood cell protein hydrolysate (BCH) were analyzed for pH value, peptide content and antioxidative properties. The significantly higher peptide contents were observed in BPH than that of BCH, which showed that blood plasma protein was more suitable to hydrolysis by alcalase than blood cell protein. Both BPH and BCH showed strong 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical‐scavenging activity and Fe²⁺ chelating ability. BPH at 4 h of hydrolysis (BPH4) demonstrated significantly higher antioxidant capacity than those treated by alcalase in most of the assays. The BPH4 was separated using ultra‐filtration and assessment of the fractions and indicated that low molecular weight of peptides (< 3 kDa) possessed greater DPPH scavenging activity, Fe²⁺ chelating ability and inhibitory activity of lipid peroxidation. These results show that BPH has the potential to be ingredients in the food industry as a replacement of synthetic antioxidants.  相似文献   

Forecasting the catch of Japanese anchovy larvae using a net survey prior to the fishing season     

Naotaka Yasue  Ryoichi Utsumi  Toyoho Gosho 《Fisheries Science》2008,74(4):938-940

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Lipid droplets are formed in 2-cell-like cells     

Asuka FURUTA  Toshinobu NAKAMURA 《The Journal of reproduction and development》2021,67(2):79

Embryonic stem (ES) cells, derived from the inner cell mass of a blastocyst, are believed to pluripotent cells and give rise to embryonic, but not extraembryonic, tissues. In mice, totipotent 2-cell stage embryo-like (2-cell-like) cells, which are identified by reactivation of murine endogenous retrovirus with leucin transfer RNA primer (MuERV-L), arise at a very few frequencies in ES cell cultures. Here, we found that a lipid droplet forms during the transition from ES cells to 2-cell-like cells, and we propose that 2-cell-like cells utilize a unique energy storage and production pathway.  相似文献   

Survey of ophthalmic disorders among captive pinnipeds in Japan     

Misato NAKAMURA  Marin MATSUSHIRO  Masatoshi TSUNOKAWA  Seiya MAEHARA  Takanori KOORIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1075

Pinnipeds have evolved enlarged ocular bulbs to catch fish in the dark. However, their large protruding eyes are easily damaged, which increases the risk of blindness and death in these animals. In captivity, ophthalmic disorders, manifested as keratitis and cataracts, are common among pinnipeds. In this study, we investigated symptoms of ophthalmic disorders in pinniped species using a questionnaire distributed to 32 zoos and aquariums throughout Japan. We conducted this study in cooperation with the Japanese Association of Zoos and Aquariums. The survey included 295 pinnipeds from four otariid species, five phocid species, and one odobenid species. Of these, 43.1% of the pinnipeds had diseases affecting the lens, cornea, and/or other eye parts. Age was positively associated with lens disorders in California sea lions, South American sea lions, and spotted seals. Conflicts and public appearances were also associated with corneal and/or lens disorders in California sea lions. Treatments were evaluated as effective for corneal disorders and conjunctivitis. The results of this study indicate that ophthalmic disorders in pinnipeds are related to the conditions of their captive environment. Aquariums and zoos should be encouraged to share information regarding optimal maintenance practices to improve the living conditions of pinnipeds.  相似文献   

Effects of feeding level of milk replacer on body growth,plasma metabolite and insulin concentrations,and visceral organ growth of suckling calves     

Mitsuru KAMIYA  Masatoshi MATSUZAKI  Hideki ORITO  Yuko KAMIYA  Yoshi‐nori NAKAMURA  Eisaku TSUNEISHI 《Animal Science Journal》2009,80(6):662-668

The objective was to evaluate effects of feeding level of milk replacer on body growth, plasma metabolite and insulin concentrations, and allometric growth of visceral organs in suckling calves. Holstein bull calves (n = 8; 3–4 days of age) were fed either a low amount (average 0.63 kgDM/day, LM) or high amount (average 1.15 kgDM/day, HM) of high protein milk replacer until they were slaughtered at 6 weeks of age. Body weight (BW) at 4, 5, and 6 weeks of age, feed intake, average daily gain, and feed efficiency were higher in the HM than LM calves. The HM group had higher plasma glucose at 3 and 4 weeks of age and insulin levels after the age of 4 weeks compared with LM calves whereas no effect was detected on plasma nonesterified fatty acid or urea nitrogen concentrations. The HM calves had greater empty body weight (EBW), viscera‐free BW and most of the organs dissected than LM calves. Relative weights (% of EBW) of liver, spleen, kidneys, and internal fat were higher, whereas head and large intestine was lower in HM than LM calves. The results suggest that increased milk feeding levels would accelerate the growth of the body and specific organs.  相似文献