The modified Johnson-Reid method--a practice model for coccidiosis monitoring in the fattening of young fowl?     

H P Schmid 《Tier?rztliche Praxis》1987,15(1):37-42

Models of coccidiosis-monitoring known from literature are described. A suitable alternative of determining the coccidia status in a broiler population is the "modified Johnson-Reid method". This method makes a standardization of diagnostic finding and evaluation possible as well as a differentiated intestinal localization and the determination of coccidia incidence severity. In addition to the microscopic screening of the caecum contents--as described by Johnson-Reid--it appears necessary to take also swabs from parts of the duodenum and jejunum (near the Meckel's diverticulum). Subsequently this method is an important help for veterinarians working with broiler production, for diagnostic institutions as well as producers of anticoccidial agents, especially in regard to its documentation rate and the resulting consequences. In this way other diagnostic efficiency data in broiler production are significantly completed.  相似文献   

Tricyclic Guanidine Alkaloids from the Marine Sponge Acanthella cavernosa that Stabilize the Tumor Suppressor PDCD4     

Tanja Grkovic  Johanna S. Blees  Magdalena M. Bayer  Nancy H. Colburn  Cheryl L. Thomas  Curtis J. Henrich  Megan L. Peach  James B. McMahon  Tobias Schmid  Kirk R. Gustafson 《Marine drugs》2014,12(8):4593-4601

A cell-based high-throughput screen that assessed the cellular stability of a tumor suppressor protein PDCD4 (Programmed cell death 4) was used to identify a new guanidine-containing marine alkaloid mirabilin K (3), as well as the known compounds mirabilin G (1) and netamine M (2). The structures of these tricyclic guanidine alkaloids were established from extensive spectroscopic analyses. Compounds 1 and 2 inhibited cellular degradation of PDCD4 with EC₅₀ values of 1.8 μg/mL and 2.8 μg/mL, respectively. Mirabilin G (1) and netamine M (2) are the first marine natural products reported to stabilize PDCD4 under tumor promoting conditions.  相似文献   

Sympathetic activation in dogs with congestive heart failure caused by chronic mitral valve disease and dilated cardiomyopathy   总被引：1，自引：0，他引：1  

W A Ware  D D Lund  A R Subieta  P G Schmid 《Journal of the American Veterinary Medical Association》1990,197(11):1475-1481

Baseline plasma norepinephrine (NE) and epinephrine (EPI) concentrations were measured in dogs with naturally acquired heart failure (HF) caused by either degenerative mitral valve disease and mitral regurgitation (MR) or idiopathic dilated cardiomyopathy (DCM). Compared with controls (clinically normal), dogs with HF had increased plasma NE concentration, which was correlated positively with clinical severity of HF. Dogs with the most severe degree of HF (New York Heart Association functional class IV) had mean NE concentration significantly (P less than 0.05) greater than that of dogs with all other functional classes of HF. Overall, mean NE concentration in dogs with DCM was greater than that in dogs with MR. Plasma EPI concentration was not different between control dogs and dogs with HF or between dogs with DCM or MR. Correlations were not found between the echocardiographically derived end systolic volume index (used as an estimate of myocardial function) and plasma NE and EPI concentrations or serum sodium or potassium concentration. Dogs with DCM, as a group, had a small but significant (P less than 0.05) decrease in serum sodium concentration, compared with dogs with MR. This difference was maintained only for class-IV HF when dogs were separated according to functional HF class. In dogs with DCM, significant inverse correlation was found between plasma NE and serum sodium concentrations. When grouped together, all dogs with HF maintained this relationship; however, dogs with MR did not have correlation between plasma NE and serum sodium concentrations. Plasma EPI and serum sodium concentrations were not correlated for any group. It was concluded that in dogs, plasma NE, but not EPI, concentration is high in relation to the clinical severity of naturally acquired HF.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

DOPPLER MEASUREMENT OF SPLANCHNIC BLOOD FLOW DURING DIGESTION IN UNSEDATED NORMAL DOGS     

Sabine  Riesen  DR MED VET  Valerie  Schmid  DR MED VET  Lorrie  Gaschen  DVM  DR MED VET  Andre  Busato  DR MED VET MSC  Johann  Lang PD  DR MED VET 《Veterinary radiology & ultrasound》2002,43(6):554-560

The purpose of this study was to measure splanchnic blood flow during digestion in unsedated dogs by using duplex Doppler sonography. The study population consisted of 12 healthy dogs. Blood flow in the cranial mesenteric artery, the celiac artery, and the aorta was measured before a test meal and at 20, 60, and 90 minutes after eating. The following measurements were made or calculated: vessel diameter, peak systolic velocity, end diastolic velocity, mean velocity, resistive index, pulsatility index, and flow volume. There was a significant postprandial decrease in the resistive and pulsatility indices in both the cranial mesenteric (preprandial RI = 0.867, postprandial RI = 0.796, preprandial PI = 3.033, postprandial PI = 2.173) and the celiac (preprandial RI = 0.854, postprandial RI = 0.769, preprandial PI = 2.639, postprandial PI = 1.930) arteries. In both vessels the end diastolic velocity, the mean velocity, and the flow volume increased significantly postprandially. These changes occurred significantly earlier in the celiac artery than in the cranial mesenteric artery. The findings most likely correspond to postprandial splanchnic vasodilation. Doppler ultrasound provide a good methode of detecting changes in postprandial splanchnic blood flow in the dog.  相似文献   

Flooding tolerance of spelt (Triticum spelta L.) compared to wheat (Triticum aestivum L.) – A physiological and genetic approach     

M. St. Burgos  M.M. Messmer  P. Stamp  J.E. Schmid 《Euphytica》2001,122(2):287-295

In marginal, agroclimatic zones, yield is often affected by flooding, but the effect is much less for winter spelt (Triticum spelta L.) than for winter wheat (Triticum aestivum L.). This study evaluates the reaction of a wheat x spelt population (F₅ RILs of Forno x Oberkulmer) to flooding stress in the early phase of germination. Lines with greater tolerance to 48 h flooding just after imbibition showed less electrolyte leakage (r = -0.79) indicating greater membrane integrity and better survival. Five QTL explaining 40.6% of the phenotypic variance for survival to flooding were found, and localized on the chromosomes 2B, 3B,5A, and 7S. The tolerance to 48 h flooding four days after sowing was best correlated with the mean germination time (r = 0.8), indicating that the plants with a fast coleoptile growth during flooding are less susceptible to flooding. Ten QTL were found for seedling growth index after flooding explaining 35.5% of the phenotypic variance. They were localized on chromosomes 2A, 2B, 2D, 3A, 4B, 5A, 5B, 6A, and 7S. Standard varieties of spelt and wheat showed the same tolerance characteristics. The possibility to use marker assisted selection for flooding tolerance is discussed. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Impacts of population growth, economic development, and technical change on global food production and consumption   总被引：3，自引：0，他引：3  

Uwe A. Schneider  Petr HavlíkErwin Schmid  Hugo ValinAline Mosnier  Michael ObersteinerHannes Böttcher  Rastislav SkalskýJuraj Balkovi?  Timm SauerSteffen Fritz 《Agricultural Systems》2011,104(2):204-215

Over the next decades mankind will demand more food from fewer land and water resources. This study quantifies the food production impacts of four alternative development scenarios from the Millennium Ecosystem Assessment and the Special Report on Emission Scenarios. Partially and jointly considered are land and water supply impacts from population growth, and technical change, as well as forest and agricultural commodity demand shifts from population growth and economic development. The income impacts on food demand are computed with dynamic elasticities. Simulations with a global, partial equilibrium model of the agricultural and forest sectors show that per capita food levels increase in all examined development scenarios with minor impacts on food prices. Global agricultural land increases by up to 14% between 2010 and 2030. Deforestation restrictions strongly impact the price of land and water resources but have little consequences for the global level of food production and food prices. While projected income changes have the highest partial impact on per capita food consumption levels, population growth leads to the highest increase in total food production. The impact of technical change is amplified or mitigated by adaptations of land management intensities.  相似文献   

Oxidation-resistant gold-55 clusters     

Boyen HG  Kästle G  Weigl F  Koslowski B  Dietrich C  Ziemann P  Spatz JP  Riethmüller S  Hartmann C  Möller M  Schmid G  Garnier MG  Oelhafen P 《Science (New York, N.Y.)》2002,297(5586):1533-1536

Gold nanoparticles ranging in diameter from 1 to 8 nanometers were prepared on top of silicon wafers in order to study the size dependence of their oxidation behavior when exposed to atomic oxygen. X-ray photoelectron spectroscopy showed a maximum oxidation resistance for "magic-number" clusters containing 55 gold atoms. This inertness is not related to electron confinement leading to a size-induced metal-to-insulator transition, but rather seems to be linked to the closed-shell structure of such magic clusters. The result additionally suggests that gold-55 clusters may act as especially effective oxidation catalysts, such as for oxidizing carbon monoxide.  相似文献   

Nitrogenase MoFe-protein at 1.16 A resolution: a central ligand in the FeMo-cofactor     

Einsle O  Tezcan FA  Andrade SL  Schmid B  Yoshida M  Howard JB  Rees DC 《Science (New York, N.Y.)》2002,297(5587):1696-1700

A high-resolution crystallographic analysis of the nitrogenase MoFe-protein reveals a previously unrecognized ligand coordinated to six iron atoms in the center of the catalytically essential FeMo-cofactor. The electron density for this ligand is masked in structures with resolutions lower than 1.55 angstroms, owing to Fourier series termination ripples from the surrounding iron and sulfur atoms in the cofactor. The central atom completes an approximate tetrahedral coordination for the six iron atoms, instead of the trigonal coordination proposed on the basis of lower resolution structures. The crystallographic refinement at 1.16 angstrom resolution is consistent with this newly detected component being a light element, most plausibly nitrogen. The presence of a nitrogen atom in the cofactor would have important implications for the mechanism of dinitrogen reduction by nitrogenase.  相似文献   

Scientific goals and experiments of the project “winter precipitation at Mount Rigi”: An overview     

J. Staehelin  A. Waldvogel  J. L. Collett Jr.  R. Dixon  R. Heimgartner  W. Henrich  C. Hsu  L. Li  L. Mosimann  B. Oberholzer  A. S. H. Prevot  W. Schmid  T. Schumann  M. Steiner  M. Volken  B. Zinder 《Water, air, and soil pollution》1993,68(1-2):1-14

Wet deposition plays an important role as a mechanism for cleansing of the polluted troposphere. Fluxes of pollutants from the atmosphere to earth's surface can affect soil and aquatic ecosystems. The investigation of the pollutant incorporation into wet deposition is therefore of considerable interest for environmental research. The project “Winter precipitation at Mount Rigi” addresses the processes determining the chemical composition of wet deposition through an integrated approach considering atmospheric physical and chemical processes and their complex interactions. The investigated topics include: aerosol and gas scavenging, atmospheric chemistry (cloud water chemistry), cloud physics and dynamics. The design of the experiment, using three ground based measuring stations at the northwestern slope of the mountain, and the conceptual ideas using case study investigations are discussed.  相似文献   

Microbial activity and bacterial composition of H₂-treated soils with net CO₂ fixation     

Susanne Stein  Rolf Schilling  Michael Schmid 《Soil biology & biochemistry》2005,37(10):1938-1945

The effects of H₂ gas treatment of an agricultural soil cultivated previously with a mixture of clover (Trifolium pratense) and alfalfa (Medicago sativa) on CO₂ dynamics and microbial activity and composition were analyzed. The H₂ emission rate of 250 nmol H₂ g⁻¹ soil h⁻¹ was similar to the upper limit of estimated H₂ amounts emitted from N₂ fixing nodules into the surrounding soil ([Dong, Z., Layzell, D.B., 2001. H₂ oxidation, O₂ uptake and CO₂ fixation in hydrogen treated soil. Plant and Soil 229, 1-12.]). After 1 week of H₂ supply to soil samples simultaneously with H₂ uptake net CO₂ production declined continuously and this finally led to a net CO₂ fixation rate in the H₂-treated soil of 8 nmol CO₂ g⁻¹ soil h⁻¹. The time course of H₂ uptake and CO₂ fixation in the soils corresponded with an increase in microbial activity and biomass of the H₂-treated soil determined by microcalorimetric measurements, fluorescence in situ hybridization analysis (FISH) and DNA staining (DAPI). Shifts in the bacterial community structure caused by the supply of H₂ were recorded. While the H₂ treatment stimulated β-and γ-subclasses of Proteobacteria, it had no significant effect on α-Proteobacteria. In addition, FISH-detectable bacteria of the Cytophaga-Flavobacterium-Bacteroides phylum increased in numbers.  相似文献