Differences in lipid peroxidation and Cu,Zn-superoxide dismutase in the hippocampal CA1 region between adult and aged dogs   总被引：2，自引：0，他引：2  

Hwang IK  Yoon YS  Yoo KY  Li H  Choi JH  Kim DW  Yi SS  Seong JK  Lee IS  Won MH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):273-277

Reactive oxygen species have been long associated with oxidative stress relevant to many pathological damages. In brain, 4-hydroxy-2E-nonenal (HNE), a major cytotoxic end product of lipid peroxidation, is produced. In contrast, superoxide dismutase (SOD), one of the major antioxidant enzymes, protects neurons from oxidative stress. The aim of this study is to observe differences in the distribution of HNE and Cu,Zn-superoxide dismutase (SOD1) in the hippocampal CA1 region of adult (2-3 years of age) and aged (10-12 years of age) dogs. The HNE immunoreactivity and protein level in the CA1 region were significantly high in the aged dogs compared to those in the adult dogs. SOD1 immunoreactivity and its protein level were also higher in the aged dogs than those in the adult dogs. However, there were not significant differences in NeuN (a neuron-specific soluble nuclear antigen) immunoreactivity in CA1 neurons between the adult and aged dogs. These differences may be associated with oxidative stress in aged dogs compared to that in adult dogs.  相似文献   

A Microbial Fermentation of Soybean and Cottonseed Meal Increases Antioxidant Activity and Gossypol Detoxification in Diets for Nile tilapia,Oreochromis niloticus     

Se‐Jin Lim  Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2011,42(4):494-503

We report the beneficial effects of a microbial fermentation process of plant protein sources. To reduce anti‐nutritional factors, soybean meal (SM) and cottonseed meal (CM) were fermented with Aspergillus oryzae, a probiotic microorganism, and a 12‐wk feeding study was conducted to evaluate the supplemental effects of the fermented soybean meal (FSM) or fermented cottonseed meal (FCM) in diets for juvenile Nile tilapia. Growth performance and hematological parameters were not influenced by the fermentation process, while the highest inclusion of both FSM and FCM lowered feed utilization efficiency. The microbial fermentation significantly increased dietary total polyphenols and consequently led to higher 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging activities in both diets and fish tissue. Dietary and liver gossypol concentrations were significantly decreased by the fermentation process of CM. The findings suggest that microbial fermentation of SM or CM with A. oryzae can enhance antioxidant activities in diets and fish, and effectively reduce or degrade the toxicity of gossypol present in CM. A microbial fermentation could make plant protein sources more useful and functional in feeds for fish.  相似文献   

Effect of capsaicin on cholecystokinin and neuropeptide Y expressions in the brain of high-fat diet fed rats     

Park ES  Jo S  Yi SJ  Kim JS  Ee HS  Lee IS  Seo KM  Sung JK  Lee I  Yoon YS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(2):107-114

Capsaicin, one of the pungent principles of hot pepper, has been reported to cause a cessation of increases in body weight and fat gain induced by high-fat feeding. Especially, in body weight and feeding control, cholecystokinin (CCK) has been well known as a satiety signal and neuropeptide Y (NPY) has been described as one of the most potent orexigenic signals. This study was carried out to investigate the effect of capsaicin on CCK- and NPY- immunoreactivities (IR) in the brain of high-fat fed rats. The animals were divided into normal-fat diet (NF), high-fat diet (HF) and high-fat diet containing capsaicin (HF-CAP) groups. Mean body weight gain (MBWG) of HF group was higher than that of NF group. However, in HF-CAP group, MBWG was lower than that of HF group. CCK-IR in suprachiasmatic nucleus (SCN), paraventricular nucleus (PVN), median eminence (ME), arcuate nucleus (ARC) and amygdala was not prominent in all the groups. In cerebral cortex, CCK-IR was more reduced in HF-CAP group than in the other groups. In the HF-CAP group, NPY-IR in the hypothalamic nuclei, amygdala and cerebral cortex was more poorly found than in the NF and HF groups. It is concluded that (1) NPY-IR may react more sensitively on capsaicin than CCK-IR, (2) no rapid increase of body weight in capsaicin treated rats may result from the diminished food intake through the low expression of NPY in hypothalamus in HF-CAP group.  相似文献   

Isolation of antioxidant compounds from orange juice by using countercurrent supercritical fluid extraction (CC-SFE).   总被引：2，自引：0，他引：2  

F J Se?oráns  A Ruiz-Rodriguez  S Cavero  A Cifuentes  E Iba?ez  G Reglero 《Journal of agricultural and food chemistry》2001,49(12):6039-6044

Antioxidants from orange juice are isolated by the use of countercurrent supercritical fluid extraction (CC-SFE) and characterized by reversed-phase liquid chromatography (RPLC) coupled to mass spectrometry (MS) and diode-array detection (DAD). A pilot-scale SFE plant equipped with a packed column has been employed for countercurrent extraction and fractionation of raw orange juice with carbon dioxide. Several experiments have been performed in order to study the effect of the countercurrent conditions on the content of antioxidative compounds. In this study, the main variable that has been considered is the solvent-to-feed ratio (S/F) because it plays an essential role in the extraction efficiency. The values tested covered a wide range of sample and solvent (CO(2)) flow rates. In each experimental run, two different extracted fractions and the residual nonextracted juice were obtained and characterized. Different flavonoids have been identified in the fractions obtained after CC-SFE. The possibility of using this process for antioxidant compounds enrichment is discussed.  相似文献   

Preliminary analysis of recombinant β-tubulin of Pseudocohnilembus persalinus (Ciliophora: Scuticociliatida) as a vaccine antigen candidate against scuticociliatosis     

Sung Mi Kim  Eun Hye Lee  Se Ryun Kwon  Sun Joung Lee  Sung Koo Kim  Yoon Kwon Nam  Ki Hong Kim   《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):21-26

The recombinant β-tubulin of Pseudocohnilembus persalinus was investigated to test whether tubulin could be a suitable antigenic target against scuticociliatosis. The cDNA of P. persalinus β-tubulin has an open reading frame of 1332 bp and had 15 TAA triplets code for glutamine (Q). All 15 TAA/Q triplets of the β-tubulin cDNA were mutated to universal CAA/Q triplets by site-directed mutagenesis and the mutated β-tubulin was expressed in Escherichia coli as a fusion with glutathione S-transferase (GST-BTU). In Western blot analysis, rat sera immunized with recombinant GST-BTU reacted with reduced β-tubulin of P. persalinus. The immunized rat sera showed higher parasiticidal activity than those of control. Ciliates preincubated with heat-inactivated immune sera showed significantly lower proliferation than ciliates preincubated with control sera when exposed to naïve rat sera as a complement source. The present results suggest that the recombinant β-tubulin of scuticociliates may be used as a target antigen for development of vaccine against scuticociliatosis.  相似文献   

绝缘避雷线抽能的研究     

刘炳尧  舒之瑜 《湖南农业大学学报(自然科学版)》1991,18(3)

According to the data of model experiment and theory analysis, this paper put forward the principle and the condition of power obtainable of modulation, and discuss the reason for that one can''t obtain the power at the presentation of resonance. The resul  相似文献   

Age-related changes in ionized calcium-binding adapter molecule 1 immunoreactivity and protein level in the gerbil hippocampal CA1 region     

Choi JH  Lee CH  Hwang IK  Won MH  Seong JK  Yoon YS  Lee HS  Lee IS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1131-1136

Microglia are evenly distributed throughout the brain parenchyma. They respond rapidly to a variety of alterations in the microenvironment of the brain and act as sensors for pathological events in the brain. In the present study, we investigated the age-dependent changes in the immunoreactivity and protein level of ionized calcium-binding adapter molecule 1 (Iba-1), a microglial marker, in the CA1 region of the gerbil hippocampus. Iba-1 immunoreactive microglia were detected in the hippocampal CA1 region of the postnatal month 1 (PM 1) group. Iba-1 positive microglia were morphologically inactive between the PM 1 and PM 12 stages. Some Iba-1 immunoreactive microglia were present in the active form in the hippocampal CA1 region of the PM 18 and PM 24 groups. The Iba-1 protein levels in hippocampal CA1 homogenates were decreased in the PM 1 through PM 6 groups and increased in an age-dependent manner thereafter. These results suggest that Iba-1 immunoreactive microglia in the active form were detected in the hippocampal CA1 region in the PM 18 and PM 24 groups. This result may be associated with an age-dependent susceptibility to neurodegenerative diseases associated with the hippocampus.  相似文献   

Clinical and Electroretinographic Findings of Progressive Retinal Atrophy in Miniature Schnauzer Dogs of South Korea     

Man Bok JEONG  Shin Ae PARK  Se Eun KIM  Young Woo PARK  Kristina NARFSTR?M  Kangmoon SEO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(10):1303-1308

  相似文献   

CPRMethicillin resistant coagulase-negative staphylococci isolated from South Korean ducks exhibiting tremor     

Jee Eun Han  Sun Young Hwang  Ji Hyung Kim  Sang Phil Shin  Jin Woo Jun  Ji Young Chai  Yong Ho Park  Se Chang Park 《Acta veterinaria Scandinavica》2013,55(1):88

Background

We describe coagulase-negative staphylococci (CoNS) isolates collected from ducklings exhibiting tremor in South Korea over the period of 2010 to 2011. Screening of antimicrobial susceptibility and analysis of SCCmec elements of CoNS were also investigated.

Results

Staphylococcus cohnii was the most frequent staphylococcus (9 isolates) and S. sciuri (4 isolates), S. lentus (3 isolate), S. simulans (1 isolate) and S. epidermidis (1 isolate) were also detected. Among the 15 antimicrobials tested in this study, resistance against oxacillin (15 isolates, 83.3%) was most frequently observed, but only one isolate (SNUDS-1) possessed mecA. This isolate was shown to possess SCCmec type III; the type 3 ccr complex and the class A mec complex.

Conclusions

Based on these results, isolate SNUDS-1 was shown to possess SCCmec type III; the type 3 ccr complex and the class A mec complex. Although the SCCmec type III is not predominant in human, MR-CoNS (Methicillin resistance Coagulase-negative staphylococci) in food animals should be monitored to prevent the dissemination of antimicrobial resistance genes and resistant pathogens to the community.  相似文献   

Genetic diversity of Korean Bacillus anthracis isolates from soil evaluated with a single nucleotide repeat analysis     

Sang Hoon Kim  Kyoung Hwa Jung  Se Kye Kim  Seong-Joo Kim  Ji-Cheon Kim  Soo Young Cho  Jin Choul Chai  Young Seek Lee  Yun Ki Kim  Hyun Chul Hwang  Sam Gon Ryu  Young Gyu Chai 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(4):457-465

Bacillus (B.) anthracis, the etiological agent of anthrax, is one of the most genetically monomorphic bacteria species in the world. Due to the very limited genetic diversity of this species, classification of isolates of this bacterium requires methods with high discriminatory power. Single nucleotide repeat (SNR) analysis is a type of variable-number tandem repeat assay that evaluates regions with very high mutation rates. To subtype a collection of 21 isolates that were obtained during a B. anthracis outbreak in Korea, we analyzed four SNR marker loci using nucleotide sequencing analysis. These isolates were obtained from soil samples and the Korean Center for Disease Control and Prevention. The SNR analysis was able to detect 13 subgenotypes, which allowed a detailed evaluation of the Korean isolates. Our study demonstrated that the SNR analysis was able to discriminate between strains with the same multiple-locus variable-number tandem repeat analysis genotypes. In summary, we obtained SNR results for four SNR marker loci of newly acquired strains from Korea. Our findings will be helpful for creating marker systems and help identify markers that could be used for future forensic studies.  相似文献