Clinical pathology of Australian bluetongue virus serotype 16 infection in Merino sheep     

M. FLANAGAN  SJ JOHNSON  D. HOFFMANN  IG POLKINGHORNE  DJ REID  MA SHEPHERD 《Australian veterinary journal》1993,70(3):101-104

SUMMARY Viraemic blood from an ox naturally infected with Australian bluetongue (BLU) virus serotype 16 was passaged twice in sheep. Twelve 2- to 4-years-old Merino ewes, negative in a bluetongue agar gel Immunodiffusion test, were Inoculated with viraemic blood from the second sheep passage. They were examined for 18 days and compared with a control group. Significant changes in haematological measurements, namely packed cell volume, total white cell count and lymphocyte count, and in plasma enzyme concentrations, namely aspartate transaminase and creatine kinase, occurred in the infected sheep. All Infected sheep became sick. The antibody response, and clinical and necropsy findings were consistent with other reports of mild to moderate disease with Australian BLU serotypes.  相似文献   

Susceptibility of cats, sheep, and swine to a rabbit isolate of Encephalitozoon cuniculi     

V F Pang  J A Shadduck 《American journal of veterinary research》1985,46(5):1071-1077

Newborn cats, pigs, and sheep (3 to 14 days old) and postweanling cats (2.5 months old) that had been inoculated with Gardner feline sarcoma virus and feline leukemia virus at 10 days of age were infected experimentally with a rabbit isolate of the mammalian protozoan parasite Encephalitozoon cuniculi. Infection occurred in all cats and in some sheep, but was questionable in pigs. Brain and kidney were the 2 major target organs in cats. The lesions were compatible with, but less severe than, those of naturally infected cats and other carnivores. Of 13 cats, E cuniculi could be detected morphologically in the kidneys of 12 cats and in the brain of 1 cat. The organisms were reisolated from 2 cats with ground tissue suspension of kidney or urine sediment. The indirect immunofluorescence antibody (IFA) titers were 1:20 to 1:1,280 at the time the animals were killed, but antibodies were not detected before inoculation. Lesions were seen in the kidneys of 2 of 4 sheep. These lesions were mild, but were compatible with those in a spontaneously affected goat. Encephalitozoon cuniculi were found morphologically in the kidney of 1 sheep with lesions. All sheep had IFA titers of 1:10 to 1:20 before inoculation, and the titers were 1:20 to 1:320 when they were killed. Vasculitis, similar to the subacute-to-chronic stage of polyarteritis nodosa, was observed in 1 of 8 pigs. The lesions were primarily present in the kidney; comparable but milder lesions were also seen in the heart and brain. Antibody was not detected before inoculation.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Granuloma formation and suspected neuropathic pain in a domestic pigeon (Columba livia) secondary to an oil‐based,inactivated Newcastle disease vaccine administered for protection against pigeon paramyxovirus‐1     

ML Cowan  DJ Monks  SR Raidal 《Australian veterinary journal》2014,92(5):171-176

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Use of REP‐PCR and 16S rRNA gene sequencing for comparison of Mannheimia haemolytica isolates obtained from fatal cases of bovine respiratory disease in the USA and Australia     

JD Taylor  DJ Doyle  PJ Blackall  AW Confer 《Australian veterinary journal》2014,92(1-2):15-23

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Diffusing-Wave Spectroscopy of Dynamics in a Three-Dimensional Granular Flow   总被引：1，自引：0，他引：1  

N Menon  DJ Durian 《Science (New York, N.Y.)》1997,275(5308):1920-1922

Diffusing-wave spectroscopy was used to measure the microscopic dynamics of grains in the interior of a three-dimensional flow of sand. The correlation functions show that minutely separated grains fly from collision to collision with large random velocities. On a time scale 10(3) to 10(4) times longer than the average time between collisions, the grains displayed slow, collective rearrangements, which, at the long-time limit, produced diffusive dynamics.  相似文献   

Freezing equine semen: the effect of combinations of semen extenders and glycerol on post-thaw motility   总被引：1，自引：1，他引：0  

J Scherzer  RA Fayrer-Hosken  M Aceves  DJ Hurley  LE Ray  L Jones  GL Heusner 《Australian veterinary journal》2009,87(7):275-279

Objective   We evaluated combinations of two commercial semen extenders and three concentrations of glycerol to determine the combination that yielded the highest post-thaw sperm motility.
Design   A randomised 2 × 3 block design was used.
Procedure   Semen was collected from four stallions (6 collections per stallion). The sample was diluted with either a dried skim-milk glucose extender (EZ Mixin Original Formula) or a chemically defined, milk-free diluent (INRA 96), and each was used in combination with 2%, 3% or 4% glycerol in standard commercial freezing medium. Sperm motility was assessed by microscopy in fresh and post-thaw semen.
Results   There was a significant difference between the two extenders in the motility of spermatozoa after cryopreservation (48.9% for INRA 96; 38.6% for EZ Mixin OF; P < 0.0001). Glycerol at 4% in freezing medium yielded the highest post-thaw motility, significantly better than 2% ( P < 0.05). Three of four stallions had significantly higher post-thaw motility using INRA 96 relative to EZ Mixin OF ( P < 0.01), and two of four stallions had significantly higher post-thaw motility using 4% glycerol ( P < 0.05). The combination of INRA 96 and 4% glycerol in freezing medium gave the highest average post-thaw motility of 51.5%.
Conclusion   In this study, INRA 96 combined with 4% glycerol yielded an average recovery of progressively motile sperm consistently above the 35% target.  相似文献   

Porcine β1,4-Galactosyltransferase-I Sequence and Expression     

EA Landers  HR Burkin  GT Bleck  L Howell-Skalla  DJ Miller 《Reproduction in domestic animals》2009,44(2):228-234

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