Antiradical function of sulfhydryl residues and oxidative stress markers in dairy cattle plasma     

Masahito TANAKA  Yuko KAMIYA  Mitsuru KAMIYA  Shigeru SHIOYA  Yutaka NAKAI 《Animal Science Journal》2007,78(1):61-65

The antiradical function of sulfhydryl (SH) residue in dairy cattle plasma and the relationship of SH residue concentrations to other oxidative stress markers, ascorbic acid and thiobarbituric acid reactive substance (TBARS) in plasma were investigated. The concentration of reduced glutathione (GSH) in phosphate buffer solution (pH 7.6) and SH residues in dairy cattle plasma decreased significantly (P < 0.05) in vitro by the addition of peroxyradicals at 38°C, depending on incubation periods. The decrease of GSH concentration with the peroxyradical solution was partially protected by the addition of sodium ascorbate solution. A positive and significant correlation with SH residues and albumin concentration in the fresh plasma obtained from 15 dairy cattle was observed (P < 0.05). The SH residue concentration was not correlated with the TBARS concentration in plasma. The total ascorbic acid and SH residues concentration in the plasma correlated positively but not significantly (P < 0.10). These results suggested that SH residues in dairy cattle plasma play important part in the antiradical function.  相似文献   

Transmission of Rhus (Rhus javanica L.) Yellows by Hishimonus sellatus and Host Range of the Causal Phytoplasma     

Minoru TANAKA  Shigeru OSADA  Izumi MATSUDA 《Journal of General Plant Pathology》2000,66(4):323-326

In 1998, rhus (Rhus javanica L.) yellows (RhY), caused by phytoplasma, was found in Miyagi Prefecture, Japan. In vector transmission tests, Hishimonus sellatus acquired RhY phytoplasma from diseased R. javanica and transmitted it to healthy R. javanica. Twenty-two species of herbaceous plants in 10 families were infected with RhY phytoplasma by H. sellatus. The host range and main symptoms on test plants of RhY phytoplasma differed from those of Macrosteles striifrons-transmitted phytoplasmas, which belong to the same 16Sr I group phytoplasma. Received 6 December 1999/ Accepted in revised form 14 May 2000  相似文献   

Characteristics of Fluid Composition of Left Displaced Abomasum in Beef Cattle Fed High-Starch Diets     

Toshihiro ICHIJO  Hiroshi SATOH  Yuki YOSHIDA  Isao MURAYAMA  Tomoko KIKUCHI  Shigeru SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1165-1167

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Prognostic Judgment at Post-Surgery by Biochemical Parameters in Beef Cattle with Left Displaced Abomasum     

Toshihiro ICHIJO  Hiroshi SATOH  Yuki YOSHIDA  Isao MURAYAMA  Kiyoshi TAGUCHI  Shigeru SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(10):1419-1421

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Immune-Stimulatory Effects of a Bacteria-Based Probiotic on Peripheral Leukocyte Subpopulations and Cytokine mRNA Expression Levels in Scouring Holstein Calves     

Abdul Qadir QADIS  Satoru GOYA  Minoru YATSU  Atsushi KIMURA  Toshihiro ICHIJO  Shigeru SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(5):677-684

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The Gs-linked receptor GPR3 maintains meiotic arrest in mammalian oocytes     

Mehlmann LM  Saeki Y  Tanaka S  Brennan TJ  Evsikov AV  Pendola FL  Knowles BB  Eppig JJ  Jaffe LA 《Science (New York, N.Y.)》2004,306(5703):1947-1950

Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype can be reversed by injection of Gpr3 RNA into the oocytes. Thus, the GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle and is crucial for the regulation of meiosis.  相似文献   

Crystal structures and structural stabilities of the disulfide bond-deficient soybean proglycinin mutants C12G and C88S     

Adachi M  Okuda E  Kaneda Y  Hashimoto A  Shutov AD  Becker C  Müntz K  Utsumi S 《Journal of agricultural and food chemistry》2003,51(16):4633-4639

The constituent subunits of seed storage protein 11S globulin have two disulfide bonds that are common among 11S globulins from legume and nonlegume seeds. In the case of the A1aB1b subunit of soybean 11S globulin, glycinin, Cys12-Cys45 and Cys88-Cys298 are observed by X-ray crystallography. The significance of these two disulfide bonds for structural stability was investigated by mutagenesis of Cys12 to Gly and of Cys88 to Ser. The disulfide bond-deficient mutants C12G and C88S could form the correct conformations identical to that of the wild-type proglycinin except in the vicinities of the mutation sites C12 and C88 as shown by their crystal structures. Thermal stability monitored by differential scanning calorimetry of the mutants indicated that the contribution of these disulfide bonds to the thermal stability of proglycinin A1aB1b is low, although there is a small difference in the extent of the contribution between the two disulfide bonds (Cys12-Cys45 > Cys88-Cys298). The contribution of Cys88-Cys298 to the resistance of proglycinin A1aB1b to proteinase digestion is higher than that of Cys12-Cys45. Possible effects of structure on the different properties of C12G and C88S are discussed.  相似文献   

Estimates of numbers of masu salmon caught by recreational fishermen in the coastal area off Iburi, Hokkaido, Japan   总被引：1，自引：0，他引：1  

Yasuyuki  MIYAKOSHI  Tatsuya  KOYAMA  Tomoya  AOYAMA  Shigeru  SAKAKIBARA  Shuichi  KITADA 《Fisheries Science》2004,70(1):87-93

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Potato yield enhancement through intensification of sink and source performances     

Akira Katoh  Hiroki Ashida  Ichiro Kasajima  Shigeru Shigeoka  Akiho Yokota 《Breeding Science》2015,65(1):77-84

The combined total annual yield of six major crops (maize, rice, wheat, cassava, soybean, and potato; Solanum tuberosum L.) amounts to 3.1 billion tons. In recent years, staple crops have begun to be used as substitutes for fossil fuel and feedstocks. The diversion of crop products to fuels and industrial feedstocks has become a concern in many countries because of competition for arable lands and increased food prices. These concerns are definitely justified; however, if plant biotechnology succeeds in increasing crop yields to double the current yields, it will be possible to divert the surplus to purposes other than food without detrimental effects. Maize, rice, wheat, and soybean bear their sink organs in the aerial parts of the plant, and potato in the underground parts. Plants with aerial storage organs cannot accumulate products beyond their capacity to support the weight of these organs. In contrast, potato has heavy storage organs that are supported by the soil. In this mini-review, we introduce strategies of intensifying potato productivity and discuss recent advances in this research area.  相似文献   

Multiple biological functions of novel basic proteins isolated from duck egg white: duck basic protein small 1 (dBPS1) and 2 (dBPS2)     

Naknukool S  Hayakawa S  Ogawa M 《Journal of agricultural and food chemistry》2011,59(9):5081-5086

Biological functions of duck basic protein small 1 (dBPS(1)) and 2 (dBPS(2)) were investigated by in vitro experiments. Results of agarose gel retardation assay indicated that dBPS(1) and dBPS(2) associate with RNA. Addition of NaCl or urea induced partial dissociation of dBPS(1)/dBPS(2)-RNA complex, implying that electrostatic interaction, hydrophobic interaction, and hydrogen bonds are involved in the association of dBPS(1)/dBPS(2) to RNA. dBPS(1) and dBPS(2) inhibited pancreatic lipase activity with the fifty percent inhibitory concentration (IC(50)) of 250 and 100 μg/mL, respectively. Peptic hydrolysates of dBPS(1) and those of dBPS(2) showed a potent angiotensin I-converting enzyme (ACE) inhibition with an IC(50) of 22.5 and 49.6 mg/L. The most potent ACE-inhibitory peptide was a nanopeptide (EKKGFCAGY) from dBPS(1) and an octapeptide (KYCPKVGY) from dBPS(2). These multiple biological functions of dBPS(1) and dBPS(2) may contribute to reducing the risk of lifestyle diseases.  相似文献