The influence of genetic determination and environment on the variability of secondary resin canal traits was investigated on 15–19-year-old Norway spruce (Picea abies (L.) Karst.) clones grown in Lower Austria (Pressbaum and Ulmerfeld) and southern Sweden (Knutstorp and Hermanstorp). Eleven and 20 clones were present on the two Austrian and the two Swedish sites, respectively. The sites differed in their water availability, as indicated by different annual precipitation and soil type. Resin canal traits measured were the number of epithelial cells per canal, the number of resin canals per unit tangential wood surface area, the mean resin canal area and the total resin canal area per unit tangential wood surface area. The latter three traits are known to be related to the constitutive resin flow of Norway spruce.
Environment had an influence on the variability of resin canal traits but the most important factor for the variability was the tree’s genetic disposition. Within countries, clones from the drier sites (Pressbaum and Hermanstorp) showed significantly smaller resin canals. Trees from Pressbaum also had smaller total resin canal areas than trees from Ulmerfeld. The number of epithelial cells and the number of canals did not differ between sites.
Resin canal traits had wide genetic variation and high broad sense heritabilities (H2), with values between 0.28 and 0.82. Highest heritability values were reached for the number of epithelial cells and the number of canals (H2 > 0.8). Genotypic correlations across trials were high for the resin canal traits and approached 1 in both the Austrian and the Swedish trials, indicating that there was little genotype by environment interaction for these traits and thus the ranking of clones was very similar in the different environments.
The number of epithelial cells, the mean area and the total resin canal area showed either moderately significant positive genotypic correlations with tree growth traits or none at all.
In a breeding context, our results are encouraging and indicate that high constitutive defence potential against bark beetles, such as Ips typographus, is not aligned with low volume growth. But it should be taken into consideration that environment and forestry practices can also have an impact on the resin reservoir provided by the radial resin canals. 相似文献
Biodiversity is declining throughout southern African miombo woodlands due to poor land use practices that are detrimental
to soil, vegetation and habitat. We aimed at examining tree and shrub species diversity and distribution in Uapaca kirkiana-dominated woodlands under three land tenure types; forest reserves, customary and leasehold land. The study was conducted
at different forest sites within similar agroecological zone in southern Malawi. Study sites were located at a range of altitudes
between 900 and 1,200 m a.s.l. Three circular plots each with 32.60 m radius were established at each study site to sample
tree and shrub species composition, structure and distribution under three land tenure types. We found that forest reserves
had higher species diversity with an average of 16 tree families, 27 genera and 34 species as compared to only 10 tree families,
6 genera and 6 species on customary forest lands. Comparisons of diameter at breast height size class distributions showed
that customary land had significantly low numbers of small (5.0–10.0 cm) and very large (≥30 cm) diameter classes suggesting
lower levels of regeneration and high rate of anthropogenic activities. The high species diversity and richness in forest
reserves and leasehold land indicate high potential for protected lands to restore tree species diversity. It is concluded
that levels of human activities as influenced by land tenure type reduce tree species diversity, composition and distribution
at the different sites, and this confirms the hypothesis that open access lands are not compatible with conservation of tree
and shrub species diversity because of high anthropogenic activities. 相似文献
Fungi cause serious problems in wood utilization, and environmentally benign wood protection is required as an alternative to traditional chemicals. Chitosan has shown promising antimicrobial properties against several microorganisms. In this study, we present the characterization of and antifungal properties of a commercial chitosan formulation developed for impregnation of wood. A broad range of chemical and mycological methods were used to evaluate the uptake, fixation, and antifungal properties of chitosan for wood preservation. The results show that the higher the uptake of chitosan the lower the relative recovery of chitosan in wood after leaching, and the higher the molecular weight of chitosan the higher the recovery. Chitosan with high molecular weight proved to be more efficient against decay fungi than chitosan with low molecular weight. The fungi tested on chitosan-amended nutrient agar medium were totally inhibited at 1% (w/v) concentration. In decay studies using small wood blocks, 4.8% (w/v) chitosan concentration gave the best protection against brown rot fungi. 相似文献
ObjectiveTo characterize the impact of mechanical positive pressure ventilation on heart rate (HR), arterial blood pressure, blood gases, lactate, glucose, sodium, potassium and calcium concentrations in rattlesnakes during anesthesia and the subsequent recovery period.Study designProspective, randomized trial.AnimalsTwenty one fasted adult South American rattlesnakes (Crotalus durissus terrificus).MethodsSnakes were anesthetized with propofol (15 mg kg−1) intravenously, endotracheally intubated and assigned to one of four ventilation regimens: Spontaneous ventilation, or mechanical ventilation at a tidal volume of 30 mL kg−1 at 1 breath every 90 seconds, 5 breaths minute−1, or 15 breaths minute−1. Arterial blood was collected from indwelling catheters at 30, 40, and 60 minutes and 2, 6, and 24 hours following induction of anesthesia and analyzed for pH, PaO2, PaCO2, and selected variables. Mean arterial blood pressure (MAP) and HR were recorded at 30, 40, 60 minutes and 24 hours.ResultsSpontaneous ventilation and 1 breath every 90 seconds resulted in a mild hypercapnia (PaCO2 22.4 ± 4.3 mmHg [3.0 ± 0.6 kPa] and 24.5 ± 1.6 mmHg [3.3 ± 0.2 kPa], respectively), 5 breaths minute−1 resulted in normocapnia (14.2 ± 2.7 mmHg [1.9 ± 0.4 kPa]), while 15 breaths minute−1 caused marked hypocapnia (8.2 ± 2.5 mmHg [1.1 ± 0.3 kPa]). Following recovery, blood gases of the four groups were similar from 2 hours. Anesthesia, independent of ventilation was associated with significantly elevated glucose, lactate and potassium concentrations compared to values at 24 hours (p < 0.0001). MAP increased significantly with increasing ventilation frequency (p < 0.001). HR did not vary among regimens.Conclusions and clinical relevanceMechanical ventilation had a profound impact on blood gases and blood pressure. The results support the use of mechanical ventilation with a frequency of 1–2 breaths minute−1 at a tidal volume of 30 mL kg−1 during anesthesia in fasted snakes. 相似文献
Hydrated gluten, treated with various salts, was analyzed by near-infrared (NIR) spectroscopy to assess the ability of this method to reveal protein structure and interaction changes in perturbed food systems. The spectra were pretreated with second-derivative transformation and extended multiplicative signal correction for improving the band resolution and removing physical and quantitative spectral variations. Principal component analysis of the preprocessed spectra showed spectral effects that depended on salt type and concentration. Although both gluten texture and the NIR spectra were little influenced by treatment with salt solutions of low concentrations (0.1-0.2 M), they were significantly and diversely affected by treatment with 1.0 M salt solutions. Compared to hydration in water, hydration in 1.0 M sulfate salts caused spectral effects similar to a drying-out effect, which could be explained by salting-out. 相似文献
Fish oil was incorporated into milk under different homogenization temperatures (50 and 72 degrees C) and pressures (5, 15, and 22.5 MPa). Subsequently, the oxidative stability of the milk and changes in the protein composition of the milk fat globule membrane (MFGM) were examined. Results showed that high pressure and high temperature (72 degrees C and 22.5 MPa) resulted in less lipid oxidation, whereas low pressure and low temperature (50 degrees C and 5 MPa) resulted in faster lipid oxidation. Analysis of protein oxidation indicated that especially casein was prone to oxidation. The level of free thiol groups was increased by high temperature (72 degrees C) and with increasing pressure. Furthermore, SDS-PAGE and confocal laser scanning microscopy (CLSM) indicated that high temperature resulted in an increase in beta-lactoglobulin adsorbed at the oil-water interface. This was even more pronounced with higher pressure. Less casein seemed to be present at the oil-water interface with increasing pressure. Overall, the results indicated that a combination of more beta-lactoglobulin and less casein at the oil-water interface gave the most stable emulsions with respect to lipid oxidation. 相似文献
OBJECTIVE: To examine the relationship between mental distress, academic performance and regular breakfast consumption across gender and immigration status. DESIGN: Cross-sectional population-based study. Two four-page questionnaires were filled in during two school sessions. SETTING: All junior high schools in Oslo, Norway using the classroom as the setting for the study. SUBJECTS: All 10th grade students 15-16 years olds in 2000 and 2001. Of 8316 eligible students, 7343 (88.3%) participated in the study. RESULTS: All immigrant groups, except the Western countries group, are skipping breakfast more often than Norwegian students, and girls more often than boys (27 versus 19%). After adjustment for possible confounding factors, the odds ratio (OR) for being mentally distressed when eating breakfast seldom/never compared with every day was 3.0 (2.0-4.5) for boys, 1.6 (1.2-2.1) for girls and 1.6 (1.5-2.6) for the immigrant group. The comparable OR for having low school grades was similar for boys and girls, 2.0 (1.3-3.0), and 1.6 (1.5-2.6) for the immigrant groups. CONCLUSIONS: Skipping breakfast is a common feature among 10th grade students. The implications of skipping breakfast on mental distress and academic performance are stronger for boys than girls and stronger for Norwegians compared with immigrants. 相似文献
A novel HPLC method was developed for detection of the Fusarium mycotoxin, moniliformin in whole maize plants. The method is based on hydrophilic interaction chromatography (HILIC) on a ZIC zwitterion column combined with diode array detection and negative electrospray mass spectrometry (ESI(-)-MS). Samples were extracted using acetonitrile-water (85:15), and the extracts were cleaned up on strong anion exchange columns. By this procedure we obtained a recovery rate of 57-74% moniliformin with a limit of detection at 48 ng/g and a limit of quantification at 96 ng/g using UV detection at 229 nm, which is comparable to current methods used. Limit of detection and quantification using ESI(-)-MS detection was 1 and 12 ng/g, respectively. Screening of maize samples infected with the moniliformin producing fungi F. avenaceum, F. tricinctum, or F. subglutinans detected moniliformin levels of 1-12 ng/g in 15 of 28 samples using ESI(-)-MS detection. To our knowledge this is the first example of HILIC separation in mycotoxin analysis. 相似文献
Previous studies have recorded Spironucleus torosus Poynton et Morrison, 1990 from several species of gadoid fishes, including the only freshwater gadoid, the burbot Lota lota (L.). Two morphologically different isolates of S. torosus have been described (elongate and pyriform). Both have been found in saltwater, while only the elongate has been found in freshwater. To address the conspecificity of the two morphs of S. torosus, and to identify the source of S. torosus in burbot in Norway, we have sequenced the small subunit ribosomal RNA (SSU rRNA) gene from 43 isolates of S. torosus from six species of gadoid fishes sampled at 15 localities in Norway, Sweden and the Baltic Sea. Phylogenetic analyses of the SSU rRNA gene sequence data recovered two major clades, one containing mainly isolates from burbot, while the other contained isolates from marine gadoid fishes only. The genetic distance (based on 25 nucleotide substitutions in 789 base pairs) separating the two assemblages was not large enough to consider the two groups separate species. Spironucleus torosus isolated from burbot displayed limited genetic variation in the small subunit ribosomal RNA (SSU rRNA) gene along the post-Pleistocene migration route of its host. The present study is the first report of S. torosus in tusk Brosme brosme (Ascanius), whiting Merlangius merlangus (L.), and fourbeard rockling Enchelyopus cimbrius (L.). 相似文献