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431.
SUN Wei ZHANG Xue-fu Ahsan Morshed Gudrun Johannsen Johannes Keizer Yves Jaques Stefano Anibald LI Nan LIU Jia-yi 《中国农业科学(英文版)》2012,11(5):823-830
For the users' convenience of accessing the AGRIS resources quickly and using them fully,the paper decomposes the structure of AGRIS Search net,analyzes the users' requirement met for conducting a bili... 相似文献
432.
We present a report of dendritic ulcerative keratitis in a 4-year old locally immunosuppressed dog suspected to result from acute primary canine herpesvirus-1 (CHV-1) infection. The dog was presented for evaluation of mild blepharospasm and conjunctival hyperemia in the right eye (OD) shortly after attending a public boarding facility. For approximately 3 months, the dog had been receiving topical prednisolone acetate 1.0% and tacrolimus 0.02% in both eyes (OU) q12h for treatment of follicular conjunctivitis. Ophthalmic examination revealed three regions of corneal fluorescein retention OD. The lesions had a dendritic pattern, were approximately 2-3 mm in length, and were located at the dorsomedial, lateral, and ventromedial aspects of the cornea. No additional abnormalities were noted on complete ophthalmic and physical examinations. CHV-1 was identified in conjunctival samples OD by polymerase chain reaction, and paired CHV-1 serum virus neutralization antibody titers were positive and consistent with acute infection. Topical prednisolone acetate and tacrolimus were discontinued. The dog was treated with cidofovir 0.5% OU q12h for a period of 4 weeks, with resolution of corneal disease noted within 1 week of treatment. In conjunction with previous studies, this case report supports a central role for alterations in host immune status in the pathogenesis and clinical manifestations of CHV-1 ocular disease in dogs. 相似文献
433.
Di Concetto S Mandsager RE Riebold TW Stieger-Vanegas SM Killos M 《Veterinary anaesthesia and analgesia》2012,39(1):99-105
Objective To investigate whether rostral extension of the hind limbs increases the cranio‐caudal dorsal interlaminar distance between the seventh lumbar vertebra and the sacral bone (LS distance) in sternally recumbent anesthetized dogs. Study design Prospective clinical study. Animals Eighteen dogs (eight neutered males, three intact males, six spayed females, one intact female) of various breeds, weighing 4–34 kg and ranging in age from 1 to 13 years. Methods Each dog was grouped by size: small (≤10 kg), medium (15–20 kg) or large (≥25 kg). Each dog was anesthetized and positioned in sternal recumbency. Computed tomography (CT) of the lumbosacral area was performed with the hind limbs resting on the stifle and the feet extended posteriorly, and then with the hind limbs extended rostrally. LS distance, craniocaudal dorsal interlaminar distance between sixth and seventh lumbar vertebra (L6–L7 distance), length of L7 vertebral body and lumbosacral angle (LS angle) were measured on a reconstructed mid‐sagittal CT image from the two hind limb positions. The measurements from the two hind limb positions for the whole dog population and by size were compared using Student’s T tests. Diagnostic interpretation of the CT images was performed. Results The length of L7 was taken as the reference value as it was not affected by hind limb position. LS distance, L6–L7 distance and LS angle were significantly higher when the hind limbs were extended rostrally in all three size groups. The CT images of ten dogs showed clinically undetected osteoarthrosis of the ileo‐ and lumbosacral area. Conclusions and clinical relevance Rostral extension of the hind limbs significantly increases LS and L6–L7 distance and LS angle even in dogs with clinically undetected osteoarthrosis of the ileo‐ and lumbosacral area, and may enhance the ease of lumbosacral epidural injection in sternally recumbent anesthetized dogs. 相似文献
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438.
Alessandra Lagomarsino Brigitte A. Knapp M. Cristina Moscatelli Paolo De Angelis Stefano Grego Heribert Insam 《Journal of Soils and Sediments》2007,7(6):399-405
Background, Aims, and Scope The genetic structure and the functionality of soil microbes are both important when studying the role of soil in the C cycle
in elevated CO2 scenarios. The aim of this work was to investigate the genetic composition of the fungal community by means of PCR-DGGE and
the functional diversity of soil micro-organisms in general with MicroResp-based community level physiological profiling (CLPP)
in a poplar plantation (POPFACE) grown under elevated [CO2] with and without nitrogen fertilization.
Materials and Methods The POPFACE experimental plantation and FACE facility are located in central Italy, Tuscania (VT). Clones of Populus alba, Populus nigra and Populus x euramericana were grown, from 1999 to 2004, in six 314 m2 plots treated either with atmospheric (control) or enriched (550 μmol mol−1) CO2 with FACE (Free Air CO2 Enrichment) technology in each growing season. Each plot is divided into six triangular sectors, with two sectors per poplar
genotype: three species × two nitrogen levels. After removal of the litter layer one soil core per genotype (10 cm wide, 20
cm depth) was taken inside each of the three sectors in each plot, for a total of 36 soil cores (3 replicates × 2 [CO2] × 2 fertilization × 3 species) in October 2004 and in July 2005. DNA was extracted with a bead beating procedure. 18S rDNA
gene fragments were amplified with PCR using fungal primers (FR1 GC and FF390). Analysis of CLPP was performed using the MicroResp
method. Carbon substrates were selected depending on their ecological relevance to soil and their solubility in water. In
particular rhizospheric C sources (carboxylic acids and carbohydrates) were chosen considering the importance of root inputs
for microbial metabolism.
Results The fertilization treatment differentiated the fungal community composition regardless of elevated [CO2] or the poplar species; moreover the number of fungal species was lower in fertilized soil. The effect of elevated [CO2] on the fungal community composition was evident only as interaction with the fertilization treatment as, in N-sufficient
soils, the elevated [CO2] selected a different microbial community. For CLPP, the differ ent poplar species were the main factors of variation. The
FACE treatment, on average, resulted in lower C utilization rates in un-fertilized soils and higher in fertilized soils.
Discussion Fungal biomass and fungal composition depend on different factors: from previous studies we know that the greater quantity
and the higher C/N ratio of organic inputs under elevated [CO2] influenced positively the fungal biomass both in fertilized and in un-fertilized soil, whereas nitrogen availability resulted
to be the main determinant of fungal community composition in this work. Whole active microbial community was directly influenced
by the soil nutrient availability and the poplar species. Under elevated CO2 the competition for N with plants strongly affected the microbial communities, which were not able to benefit from added
rhizospheric substrates. Under Nsufficient conditions, the increase of microbial activity due to [CO2] enrichment was related to a more active microbial community, favoured by the current availability of C and N.
Conclusions Different factors influenced the microbial community at different levels: poplar species and root exudates affected the functional
properties of the microbial community, while the fungal specific composition (as seen with DGGE) remained unaffected. On the
other hand, factors such as N and C availability had a strong impact on the community functionality and composition. Fungal
community structure reflected the availability of N in soils and the effect of elevated [CO2] on community structure and function was evident only in N-sufficient soils. The simultaneous availability of C and N was
therefore the main driving force for microbial structure and function in this plantation.
Recommendations and Perspectives Using the soil instead of soil extracts for CLPP determination provides a direct measurement of substrate catabolism by microbial
communities and reflects activity rather than growth because more immediate responses to substrates are measured. Further
applications of this approach could include selective inhibition of different microbial functional groups to investigate specific
CLPPs. To combine the structural analysis and the catabolic responses of specific microbial communities (i.e. fungi or bacteria)
could provide new outlooks on the role of microbes on SOM decomposition.
ESS-Submission Editor: Dr. Kirk Semple (k.semple@lancaster.ac.uk) 相似文献
439.
The epiphytic lichen Parmelia caperata was used systematically to biomonitor trace elements in the Travale-Radicondoli geothermal field (central Italy). The results showed a very low occurrence of potentially toxic heavy metals such as Cd, Hg and Pb, and relatively high values for As, B, Mo and elements associated with soil dust (Al, Co, Cr, Fe, Mn and Ni). Boron was the principal element of geothermal origin. Arsenic seems to originate both from power plants and adjacent thermal springs. It is concluded that trace element pollution in the area is low. 相似文献
440.
Sacchetti G Medici A Maietti S Radice M Muzzoli M Manfredini S Braccioli E Bruni R 《Journal of agricultural and food chemistry》2004,52(11):3486-3491
Wild Amazonian basil Ocimum micranthum Willd. (O. campechianum Mill.) Labiatae essential oil was analyzed by GC and GC-MS: 31 compounds were identified. The main components were eugenol (46.55 +/- 5.11%), beta-caryophyllene (11.94 +/- 1.31%), and beta-elemene (9.06 +/- 0.99%), while a small amount of linalool (1.49 +/- 0.16%) was detected. The oil was tested for its in vitro food-related biological activities and compared with common basil Ocimum basilicum and Thymus vulgaris commercial essential oils. Radical scavenging activity was evaluated employing 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The oil exerted a good capacity to act as a nonspecific donor of hydrogen atoms or electrons when checked in the diphenylpicrylhydrazyl assay, quenching 76,61 +/- 0.33% of the radical, with values higher than those reported by reference oils. In the beta-carotene bleaching test, the oil provided an antioxidant efficacy comparable with that of O. basilicum and T. vulgaris essential oils. These data were confirmed by photochemiluminescence, where the oil showed a remarkable antioxidant capacity (2.39 +/- 0.1), comparable to that of Trolox and vitamin E, and higher than the other essential oils. Antibacterial activity of O. micranthum essential oil was evaluated against Gram positive and Gram negative bacterial strains. The oil showed a dose-dependent antifungal activity against pathogenic and food spoiling yeasts. 相似文献