小麦对条锈病的水平抗病性研究初报   总被引：4，自引：0，他引：4  

曾士迈  王沛有  武修英  张万义  王吉庆  宋位中  王生元 《植物保护学报》1979,6(1):1-10

将小麦品种采用随机区组的田间设计种于小种病圃中,以鉴定和测定其水平抗性。根据各个“品种——小种”组合的相对病情指数的方差分析的结果,有些品种,如农大311、西农6028和丰产3号,其品种——小种互作高度显著,被鉴定为具有垂直抗性,另外一些品种,对试验所用的小种并无专化性,并且在大田生产中已显出20多年的持久的中度抗性,初步推测为属水平抗性。在后一类中,有些品种,如平原50,表现为中等反应型,其余则属于呈典型感病的迟锈慢锈类型。在迟锈慢锈品种的病指的对数矫正值(logits)和病圃的锈病强度(以感病对照品种的病指表之)的对数矫正值之间发现了直线回归。  相似文献   

Statistical study on the predisposition to summer sterility in Holstein bulls born in Hokkaido]     

T Ishikawa  W K Sung 《The Japanese journal of veterinary research》1975,23(2):45-59

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Malignant catarrhal fever-like disease in sheep after intranasal inoculation with ovine herpesvirus-2.     

Hong Li  Donal O'Toole  Okjin Kim  J Lindsay Oaks  Timothy B Crawford 《Journal of veterinary diagnostic investigation》2005,17(2):171-175

A malignant catarrhal fever (MCF)-like disease was induced experimentally in 3 sheep after aerosol inoculation with ovine herpesvirus-2 (OvHV-2). Each of 3 OvHV-2-negative sheep was nebulized with 2 ml of nasal secretions containing approximately 3.07 X 10(9) OvHV-2 DNA copies from a sheep experiencing an intensive viral-shedding episode. Ovine herpesvirus-2 DNA became detectable by polymerase chain reaction in the peripheral blood leukocytes of all 3 sheep within 3 days, and all 3 seroconverted between 6 and 8 days postinfection (PI). The sheep developed clinical signs, with copious mucopurulent nasal discharge and fever around 14 days PI. One of the 3 clinically affected sheep was euthanized at 18 days PI. Major lesions at necropsy were multifocal linear erosions and ulcers in mucosa of the cheeks, tongue, pharynx, and proximal esophagus and mild disseminated pneumonia. Microscopically, there was extensive moderate superficial histiocytic-lymphocytic rhinitis with epithelial dissociation and degeneration. Moderate multifocal histiocytic bronchointerstitial pneumonia was associated with loss of terminal bronchiolar epithelium. Lymphocytic vasculitis was present only in the lung. The remaining 2 sheep recovered clinically, approximately 25 days PI. The study revealed that clinical signs and lesions resembling MCF can develop when uninfected sheep are exposed to a high dose of aerosolized OvHV-2.  相似文献   

韩国一次性收获的红椒品种—Saeng-ryeog216     

M.C.Cho  D.H.pae  Y.S.Cho  Y.Chae  W.M.Lee  D.H.Kim 《辣椒杂志》2003,(4)

根据栽培面积以及为农民提供的收入而言,红辣椒在韩国一直是仅次于水稻而居第二位的重要农作物。按人均计算,韩国人均年辣椒粉的消费量达2.5kg以上,可能是世界最高记录。辣椒在韩国主要用作泡菜(韩国称之为Kimchi)香辛料和菜肴中的  相似文献   

农杆菌介导的蝴蝶兰基因转化系统的建立   总被引：9，自引：1，他引：9  

柴明良  金斗焕 《园艺学报》2004,31(4):537-540

 针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB)，与含绿色荧光蛋白基因( )和潮霉素磷酸转移酶基因(^p￡)的pCAMBIA 1300一SmGFP的根瘤农杆菌LBA4404 共培养，培育出了转基因的蝴蝶兰。经绿色荧光蛋白检测和Southern印迹，证实了再生植株中含cop基因和hpt基因。  相似文献   

Antiviral Effect of Saccharomyces cerevisiaeβ‐glucan to Swine Influenza Virus by Increased Production of Interferon‐γ and Nitric Oxide     

K. Jung  Y. Ha  S.‐K. Ha  D. U. Han  D.‐W. Kim  W. K. Moon  C. Chae 《Zoonoses and public health》2004,51(2):72-76

The aim of these experiments was to investigate the potential antiviral effect of Saccharomyces cerevisiaeβ‐glucan on the pneumonia induced by swine influenza virus (SIV). Forty colostrum‐deprived 5‐day‐old piglets were randomly divided into four groups of 10. The 20 pigs in groups 1 and 2 were administered Saccharomyces cerevisiaeβ‐glucan orally (50 mg/day/pig; En‐Bio Technology Co., Ltd) for 3 days before SIV infection and those in groups 3 and 4 were given culture medium/diluent alone. Groups 1 and 3 were inoculated intranasally with 3 ml of tissue culture fluid containing 2 × 10⁶ tissue culture infective doses 50% (TCID₅₀)/ml of SIV and those in groups 2 and 4 were exposed in the same manner to uninfected cell culture supernatant. The microscopic lung lesions induced by SIV infection (group 1 pigs) were significantly more severe than those induced by infection in animals pre‐administered β‐glucan (group 3) (P < 0.05). Significantly more SIV nucleic acid was detected in the lungs of pigs experimentally infected with SIV only (group 1) at 5, 7 and 10 days post‐inoculation (dpi) compared with lungs from pigs pre‐administered β‐glucan and infected with SIV (group 3) (P < 0.05). The concentrations of interferon‐γ (IFN‐γ) and nitric oxide (NO) in bronchoalveolar lavage fluid from pigs pre‐administered β‐glucan and infected with SIV (group 3) were significantly higher than for any other group at 7 and 10 dpi for IFN‐γ, and at 5, 7 and 10 dpi for NO (P < 0.05). Saccharomyces cerevisiaeβ‐glucan reduced the pulmonary lesion score and viral replication rate in SIV‐infected pigs. These findings support the potential application of β‐glucan as prophylactic/treatment agent in influenza virus infection.  相似文献   

Analysis of a Secreted Aspartic Peptidase Disruption Mutant of Glomerella cingulata     

Kim M. Plummer  Sarah J. Clark  Lana M. Ellis  Ashwini Loganathan  Taha H. Al-Samarrai  Erik H.A. Rikkerink  Patrick A. Sullivan  Matthew D. Templeton  Peter C. Farley 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):265-274

Peptidases have been implicated in the pathogenicity of fungi that cause disease in plants. Expression of the secreted aspartic peptidase gene (gcsap), of a Glomerella cingulata isolate pathogenic on apples, is induced during appressorium formation. To determine whether the secreted aspartic peptidase (GcSAP) is essential to pathogenicity, gcsap was disrupted using a vector containing a 637 bp fragment of genomic DNA that encodes the sequence spanning the two active site aspartic acid (Asp) residues. To ensure that the truncated gcsap gene products could not have residual peptidase activity the codons for the active site residues Asp¹¹² and Asp²⁹⁷ were both mutated to histidine residues. Both PCR and Southern analysis confirmed disruption of gcsap. Neither gcsap mRNA nor GcSAP activity was detected in the disruption mutant. Pathogenicity tests on fruit from three apple cultivars showed that GcSAP was not required for pathogenicity. The disruption mutant grew on medium containing protein as the sole source of nitrogen because G. cingulata secretes a previously undetected peptidase(s). A serine peptidase that had a pH optimum between pH 7.0 and 8.0 and a K _m of 0.25 mM for the synthetic substrate succinyl-Ala–Ala–Pro–Phe-p-nitroanilide was identified.  相似文献   

遗传标记及其在家禽育种中的应用   总被引：1，自引：1，他引：1  

M.G.Emara  H.Kim  吴艳 《中国家禽》2005,27(7):41-42

1 SNP SNP是染色体中遗传变异的最普通形式,如在人体已经检测基因组DNA中每1,000到2,000个单位有1个SNP,在某些区域估计每300个单位有一个SNP.基于SNP的高分辨率,遗传图谱在一些人的染色体上通常没有得到发展.正如其名称所指,SNP是发生在基因(启动子、外显子、或内含子)或基因之间(基因间隔区)的单个碱基改变或核苷酸变异.编码序列中的SNP以同义(不会导致氨基酸改变)或非同义(导致氨基酸改变)来分类.非同义SNP由于对蛋白质表达及最终对表现型的潜在影响而受到重视.相反,同义SNP可能对基因表达有微小的影响(除那些在启动子和其它基因组区域中,对DNA-蛋白质作重要的核苷酸或与RNA稳定性有关的核苷酸).同义和非同义SNP在基因图谱研究中均是很好的遗传标记.  相似文献   

Pharmacokinetic profiles of the two major active metabolites of metamizole (dipyrone) in cats following three different routes of administration          下载免费PDF全文

B. Lebkowska‐Wieruszewska  T. W. Kim  B. Chea  H. Owen  A. Poapolathep  M. Giorgi 《Journal of veterinary pharmacology and therapeutics》2018,41(2):334-339

This study was performed to determine pharmacokinetic profiles of the two active metabolites of the analgesic drug metamizole (dipyrone , MET), 4‐methylaminoantipyrine (MAA), and 4‐aminoantipyrine (AA), after intravenous (i.v., intramuscular (i.m.), and oral (p.o.) administration in cats. Six healthy mixed‐breed cats were administered MET (25 mg/kg) by i.v., i.m., or p.o. routes in a crossover design. Adverse clinical signs, namely salivation and vomiting, were detected in all groups (i.v. 67%, i.m. 34%, and p.o. 15%). The mean maximal plasma concentration of MAA for i.v., i.m., and p.o. administrations was 148.63 ± 106.64, 18.74 ± 4.97, and 20.59 ± 15.29 μg/ml, respectively, with about 7 hr of half‐life in all routes. Among the administration routes, the area under the plasma concentration curve (AUC) value was the lowest after i.m. administration and the AUC_EV/i.v. ratio was higher in p.o. than the i.m. administration without statistical significance. The plasma concentration of AA was detectable up to 24 hr, and the mean plasma concentrations were smaller than MAA. The present results suggest that MET is converted into the active metabolites in cats as in humans. Further pharmacodynamics and safety studies should be performed before any clinical use.  相似文献   

Effects of oral administration of different dosages of carvacrol essential oils on intestinal barrier function in broilers          下载免费PDF全文

ShuDong Liu  MinHo Song  Won Yun  JiHwan Lee  ChangHee Lee  WooGi Kwak  NamSoo Han  HyeunBum Kim  JinHo Cho 《Journal of animal physiology and animal nutrition》2018,102(5):1257-1265

Essential oils are widely used in the pharmaceutical, food and cosmetic industries, and many plant essential oils have shown that they have positive effects on broilers nutrition. This experiment was conducted to study the effects of orally administered different dosages of carvacrol essential oils on intestinal barrier function in broiler chickens. A total of eighty 28‐day‐old (1.28 ± 0.15 kg) ROSS 308 broilers were randomly allocated to four groups of 20 replicates each, with one chicken per replicate per cage, and all were fed with the same diet. Four experimental groups were orally administered 0, 200, 300 or 400 μl carvacrol essential oils at 18:00 hr every day during the 2‐week experimental period. As a result of which, the gene expression of the occludin, claudin‐1, claudin‐5, ZO‐1 and ZO‐2 in intestinal mucosa of small intestine (p < 0.05) and the goblet cell content in small intestine epithelium (p < 0.05) were significantly increased; test subjects with 300 or 400 μl carvacrol essential oils reduced the microbial counts of Salmonella spp. and Escherichia coli in the intestines (p < 0.05); Essential oils administration also significantly increased activity of the sucrase (p < 0.05) and lactase (p < 0.05) in intestinal mucosa. In conclusion, the carvacrol essential oils have positive effects on growth performance and intestinal barriers function of broilers; those effects may be related to the dosage, as administration of 300 or 400 μl was more effective than that of 200 μl.  相似文献