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为探究肌肉生长抑制素(myostatin,MSTN)对牛骨骼肌生长发育的作用机制,本研究前期利用定量蛋白质组学与磷酸化蛋白质组学分析野生型蒙古牛(MG.WT)和MSTN+/-蒙古牛(MG.MSTN+/-)腿臀肌肌肉组织中蛋白质水平和磷酸化修饰水平的差异变化,使用已建立的牛骨骼肌卫星细胞体外诱导成肌分化模型,检测设计合成的MSTN siRNA (si-MSTN)干扰效果;采用实时荧光定量PCR和Western blotting方法检测转染si-MSTN的增殖期(GM)和分化第3天(DM3)牛骨骼肌卫星细胞中肌动蛋白细胞骨架调节通路相关基因的mRNA和蛋白水平的表达变化,研究敲低MSTN表达对肌动蛋白细胞骨架调节通路的影响。结果显示,在MSTN+/-蒙古牛肌肉组织中共鉴定到16个肌动蛋白细胞骨架调节通路相关基因表达丰度上调;转染si-MSTN细胞中的MSTN表达水平极显著降低(P<0.01);在转染si-MSTN的GM期牛骨骼肌卫星细胞中,肌动蛋白细胞骨架调节通路相关基因ENAH、ACTN4和Cdc42的mRNA水平均显著升高(P<0.05),PFN1、RhoA和ACTN4的蛋白水平均显著或极显著升高(P<0.05;P<0.01);在转染si-MSTN的DM3牛骨骼肌卫星细胞中,ENAH、CFL1、SCIN和Cdc42基因mRNA水平均显著升高(P<0.05),RhoA基因mRNA水平极显著升高(P<0.01),PFN1和ACTN4的蛋白水平均显著升高(P<0.05)。结果表明,干扰MSTN可以促进肌动蛋白细胞骨架调节通路相关基因的表达,探明了MSTN可能通过介导肌动蛋白细胞骨架调节通路影响牛骨骼肌卫星细胞增殖和成肌分化的分子机制,为进一步研究MSTN对牛成肌分化的调控机制提供参考。 相似文献
66.
综述了非离子表面活性剂对反刍动物瘤胃微生物数量、酶活性、瘤胃发酵特性、饲料消化率和生产性能等方面的影响作用以及与其他饲料添加剂的互作效应,并就其作用机制进行了阐述。 相似文献
67.
Junko Matsuki Tomoya Okunishi Hiroshi Okadome Keitaro Suzuki Koichi Yoza Ken Tokuyasu 《Cereal Chemistry》2015,92(5):487-490
A simple method employing a commercially available canister was developed for the determination of mode of water absorption of rice flour samples. The samples prepared by four different grinding methods were used to analyze water absorption. The total amount of water in a flour sample was described by using an exponential model. Capacity and rate of water absorption of the samples were determined, and the relationship to baking quality of partially substituted rice bread was investigated. The water absorption was highly dependent on the method of grinding. Flours produced by wet jet‐milling of the grains, which absorbed a small amount of water at high speed, were most suitable for rice bread. The method was applicable to other food powders, provided that flour particles do not stick together or swell immediately upon contact with water. 相似文献
68.
Effects of extracerebral dopamine on salsolinol‐ or thyrotropin‐releasing hormone‐induced prolactin (PRL) secretion in goats
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Yuki Inaba Yuki Kato Azumi Itou Aoi Chiba Ken Sawai Ferenc Fülöp György Miklos Nagy Tsutomu Hashizume 《Animal Science Journal》2016,87(12):1522-1527
The aim of the present study was to clarify the effect of extracerebral dopamine (DA) on salsolinol (SAL)‐induced prolactin (PRL) secretion in goats. An intravenous injection of SAL or thyrotropin‐releasing hormone (TRH) was given to female goats before and after treatment with an extracerebral DA receptor antagonist, domperidone (DOM), and the PRL‐releasing response to SAL was compared with that to TRH. DOM alone increased plasma PRL concentrations and the PRL‐releasing response to DOM alone was greater than that to either SAL alone or TRH alone. The PRL‐releasing response to DOM plus SAL was similar to that to DOM alone, and no additive effect of DOM and SAL on the secretion of PRL was observed. In contrast, the PRL‐releasing response to DOM plus TRH was greater than that to either TRH alone or DOM alone and DOM synergistically increased TRH‐induced PRL secretion. The present results demonstrate that the mechanism involved in PRL secretion by SAL differs from that by TRH, and suggest that the extracerebral DA might be associated in part with the modulation of SAL‐induced PRL secretion in goats. 相似文献
69.
Toru TACHIBANA Ken Takeshi KUSAKABE Sayuri OSAKI Takeshi KURAISHI Shosaku HATTORI Midori YOSHIZAWA Chieko KAI Yasuo KISO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):161-165
The New World monkey Aotus spp. (night monkeys) are expected
for use of valuable experimental animal with the close species of Saimiri
spp. (squirrel monkeys). Saimiri is known to show spontaneous
hypercortisolemia, although few reports in Aotus. We compared basic
states of blood steroid hormones and histological structure of the adrenal glands in two
monkeys. Serum cortisol and ACTH levels were statistically lower in Aotus
than Saimiri. Conversely, Aotus adrenocortical area
showed significant enlargement, especially at the zona fasciculata. Electron microscopic
observation at Aotus fasciculata cells revealed notable accumulation of
large lipid droplets and irregular shapes of the mitochondrial cristae. These results
suggest potential differences in cellular activities for steroidogenesis between
Aotus and Saimiri and experimental usefulness in
adrenocortical physiology and pathological models. 相似文献
70.
Hiroshi BANNAI Manabu NEMOTO Koji TSUJIMURA Takashi YAMANAKA Ken MAEDA Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):309-311
To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4
(EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer:
MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more
strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera
from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%),
whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer
ELISA. 相似文献