辣椒素的抑菌作用及其对某些保护酶活性的影响     

王建明  梁建根  陈燕飞  高俊明  张作刚 《植物病理学报》2005,35(Z1):35-37

 本文对辣椒素的抑菌作用和对寄主体内3种保护酶活性的影响进行了研究,结果表明3.57μg/mL-50.00μg/mL的辣椒素对辣椒枯萎病菌的菌丝生长都有明显的抑制作用,浓度愈高,抑制作用愈强。20μg/mL辣椒素对另外供试的8种病原菌的菌丝具有强烈的抑制作用,其中对番茄灰霉病菌抑制作用最强。辣椒素处理辣椒后1-6 d内,SOD、POD和CAT酶活性的平均增减比率值均明显高于对照,而且对根部和叶部3种保护酶活性的影响显著不同。  相似文献   

鸡源耐安普霉素大肠杆菌耐药表型研究     

高允乐  张秀英  底佳芳  陶娅 《中国兽药杂志》2005,39(9):9-11

进行了安普霉素耐药大肠杆菌耐药表型的研究.采用常规方法和生化鉴定管对有过安普霉素用药史的鸡场分离的鸡源病原性大肠杆菌进行鉴定,并用试管二倍稀释法测定其最低抑菌浓度(MIC),筛选出鸡源安普霉素耐药大肠杆菌;采用药敏纸片法研究了这些耐药菌对安普霉素等14种抗菌药物的敏感性.共筛选出7株对安普霉素耐药的鸡源性大肠杆菌,这些耐药菌株全部对安普霉素、妥布霉素、奈啶酸、多西环素和阿莫西林耐药;大部分对庆大霉素、链霉素、卡那霉素、壮观霉素也呈现耐药.对新霉素的耐药较低,对阿米卡星高度敏感.部分交叉耐药现象的存在揭示对安普霉素等氨基糖苷类药物产生耐药性的菌株,其他抗生素也可能对它们失去疗效.  相似文献   

Effects of homocysteine on number and activity of endothelial progenitor cells from peripheral blood     

WANG Xing-xiang  SHANG Yun-peng  ZHU Jun-hui  CHEN Jun-zhu  ZHU Jian-hua  TAO Qian-min  GUO Xiao-gang  WANG Zhan-kun  ZHANG Li 《园艺学报》2005,21(7):1274-1278

AIM: To investigate whether homocysteine (Hcy) has influences on endothelial progenitor cell (EPCs) number and activity from peripheral blood. METHODS: Total mononuclear cells (MNCs) were plated on fibronectin-coated culture dishes and cultured for 7 days, and then attached cells were stimulated with Hcy or vehicle control for 6 h, 12 h, 24 h and 48 h. The adhesion, proliferation, migration and in vitro vasculogenesis activity of EPCs were assayed, respectively. RESULTS: Incubation of isolated human MNCs with Hcy dose and time-dependently decreased the number of EPCs with maximum at 200 μmol/L for 24 hours (35.7±6.7 vs 62.5±10.6， P＜0.01). In addition, Hcy impaired EPC proliferative (0.531±0.061 vs 0.328±0.055， P＜0.05), migratory (26.3±6.4 vs 6.4±3.7， P＜0.01), adhesive (33.1±8.1 vs 17.4±7.5， P＜0.01) and vasculogenesis capacity (25.4±9.1 vs 10.4±4.7， P＜0.01) in a dose and time-dependent manner. CONCLUSION: It is suggested that Hcy may result in the reduction of EPCs and decrease EPC functional activity.  相似文献   

Antisense c-myc sensitizes osteosarcoma cells to cisplatin-induced apoptosis     

XIE Xian-kuan  YANG Di-sheng  YE Zhao-ming  TAO Hui-min 《园艺学报》2005,21(7):1326-1330

AIM: To down-regulate expression of c-myc through antisense therapy and to investigate its effect on the sensitivity of osteosarcoma MG-63 cells to cisplatin-induced apoptosis. METHODS: The recombinant adenovirus (Ad-Asc-myc) encoding antisense c-myc fragment was constructed and transfected into osteosarcoma MG-63 cells in vitro in order to down-regulate the expression of c-myc, and the change in the sensitivity to cisplatin-induced apoptosis was observed. MTT, Western blot, RT-PCR, flow cytometry (FCM) and electron microscope were used to evaluate tumor cell proliferation in vitro, genes expression related to apoptosis regulation and effects on the sensitivity of osteosarcoma MG-63 cells to cisplatin-induced apoptosis. RESULTS: Ad-Asc-myc down-regulated the expression of c-myc protein after transfected MG-63 cells for 48 h, combined with the treatment of 2.0 mg/L cisplatin for 2 h inhibited tumor cell proliferation in vitro by 38.0%. RT-PCR revealed that Ad-Asc-myc down-regulated the expression of Bcl-2 and up-regulated the expression of Bax. No appreciable change was observed in the expression of E2F-1. FCM showed that Ad-Asc-myc induced apoptosis in intransfected cells, and rendered it more sensitive to cisplatin. CONCLUSION: Antisense c-myc is able per se to induce apoptosis and sensitize osteosarcoma cells to cisplatin-induced apoptosis.  相似文献   

Manumycin inhibits activity of pancreatic duct cancer cell line Panc-1 via inducing apoptosis     

YANG Hui-ling  TAO Xiang  YANG Lin  ZHENG Qin 《园艺学报》2005,21(10):1888-1891

AIM: In this study, we investigated the anticancer effect and mechanisms of manumycin on pancreatic cancer cell line-Panc-1 and the role of p38MAPK pathway in apoptosis. METHODS: The test of anticancer effect was performed by MTT assay. Apoptosis was induced in the cells by manumycin and then treated with SB203580, a specific p38MAPK inhibitor. A quantitative caspase-3 activity assay kit was used in this experiment. RESULTS: Manumycin (6 μmol/L, 18 μmol/L, 54 μmol/L) significantly inhibited cell growth of pancreatic cancer cell line Panc-1. The inhibition rates 24 h after treatment with 6 μmol/L, 18 μmol/L and 54 μmol/L manumycin were 8.9%, 21.9% and 67.0%, respectively. Compared with the control group, the survival levels of the last two groups were of significant statistical difference (P<0.01). The anticancer effects also showed dosage-effect relationship, the value of IC50 24 h after treatment was 34.7 μmol/L. In addition, this reagent simultaneously activated caspase-3 protein, which was partly blocked by p38MAPK specific inhibitor, SB203580. CONCLUSION: Manumycin exerted anticancer effect on Panc-1 cell line via inducing cell apoptosis, which was partly regulated by p38MAPK.  相似文献   

西北干旱区水资源利用与生态环境重建研究   总被引：39，自引：32，他引：39  

陶希东  石培基  李鸣骥 《干旱区研究》2001,18(1):18-22

西北干旱地区是我国西部大开发战略的重点建设区域之一。但是长期以来，由于气候干旱以及在市场经济利益驱动下所形成的人类对水产 过度开发利用，导致了一系列阻碍区域经济发展的严重生态环境问题。水资源的合理利用是干旱区振兴流域经济的主要保证，也是促使生态经济系统向良性方向发展的关键因素。本文从分析西北干旱区内陆河流域水资源系统特征、开发利用现状以及主要的水环境问题出发，得出干旱区生态恢复与重建必须以水资源的合理利用为核心的结论，同时提出了干旱区水资源利用优化途径与生态重建的基本对策。  相似文献   

铝中毒对蛋鸡白细胞和红细胞免疫功能的影响   总被引：4，自引：0，他引：4  

刘福堂  李艳飞  顾庆云 《中国畜牧兽医》2007,34(10):70-72

采用连续腹腔注射相同体积不同浓度梯度的三氯化铝，建立不同程度的鸡亚慢性铝中毒型，检测铝中毒雏鸡外周血白细胞数(WBC)、淋巴细胞总数、ANAE+T数及红细胞C3b受体花环率和红细胞免疫复合物花环率。结果表明，铝中毒雏鸡外周血白细胞数、淋巴细胞总数、ANAE+T数及红细胞C3b受体花环率均明显低于健康对照组雏鸡，而红细胞免疫复合物花环率明显高于健康对照组雏鸡。上述结果说明, 铝中毒对雏鸡细胞免疫和非特异性免疫功能有明显的抑制作用。  相似文献   

畜禽内源氨基酸代谢及测定方法学研究进展   总被引：1，自引：0，他引：1  

陶青燕  王康宁 《中国畜牧杂志》2006,42(17):44-46

内源氨基酸的排泄量是计算饲料真可消化率以及析因法估计动物蛋白质、氨基酸需要量的重要参数。本文综述了近年来内源氨基酸代谢以及测定方法学的国内外研究进展,特别是我国科技工作者在准确测定动物内源氨基酸代谢方面的研究成果。  相似文献   

阿苯达唑和奥芬达唑对体外培养猪囊尾蚴能量代谢变化的影响   总被引：1，自引：0，他引：1  

高学军  李艳飞  李庆章 《中国兽医学报》2006,26(1):71-73

测定了在阿苯达唑和奥芬达唑作用下体外培养猪囊尾蚴磷酸烯醇式丙酮酸羧激酶（PEPCK）、丙酮酸激酶（PK）、延胡索酸还原酶（FR）、苹果酸酶（ME）活性的变化及葡萄糖（GLC）、乳酸（LAC）含量的变化。结果表明，2种药物作用均可在体外作用条件下显著改变未成熟期及成熟期猪囊尾蚴的能量代谢。提示，苯并咪唑氨基甲酸酯类药物的作用机理可能是通过干扰虫体能量代谢，阻断能量的产生，导致虫体死亡。  相似文献   

不同氟水平对生长育肥猪毒性的研究     

陶新  许梓荣  汪以真 《中国兽医学报》2006,26(1):91-93

96头始重(24.14±1.12)kg的杜长大三元杂交猪,随机分为4组。第1组为对照组,饲喂基础日粮(含氟量37.39mg/kg),第2、3、4组分别饲喂含氟(以NaF形式添加)量为100、150、200mg/kg的日粮,研究日粮中不同氟水平对猪生长性能和血清某些生化指标的影响,并探讨了氟在肝、肾和肌肉中的沉积,饲料氟和粪氟、血清氟、尿氟之间的相关关系。结果表明,第3、4组猪增重与对照组相比分别降低8.86%(P<0.05)和9.81%(P<0.05),料重比分别升高7.36%(P<0.05)和8.07%(P<0.05)。第4组血清钙、镁、白蛋白含量和碱性磷酸酶的活力与对照组相比分别降低了17.33%(P<0.01)、29.91%(P<0.01)、10.75%(P<0.05)和33.71%(P<0.01)。第3、4组肝、肾和肌肉氟含量与对照组相比显著增加(P<0.05)。各试验组粪氟、血清氟、尿氟含量随饲料中氟含量的增加而升高,且各组间差异极显著(P<0.001),饲料氟与粪氟、血清氟、尿氟含量相关系数分别为0.943(P<0.01)、0.911(P<0.01)和0.959(P<0.01)。  相似文献