OBJECTIVES: (1) To describe promotional activities, particularly student-led, targeting lower-fat à la carte foods that were conducted in secondary schools; and (2) to describe the relationships between the number and duration of total promotional activities for lower-fat à la carte foods and cafeteria sales of such foods over two years. DESIGN: Promotional activities were implemented in schools that were randomised to the intervention condition of a larger, two-year, school-based, randomised, controlled nutrition intervention trial. SETTING: Ten Minnesota secondary schools. SUBJECTS: Students and school faculty, school food-service and research staff (measured at the school level). RESULTS: Over two years, 181 promotions were implemented (n=49 in Year 1 and n=132 in Year 2). In Year 1, the number of promotions conducted in schools was significantly associated with percentage lower-fat food sales. In Year 2, the duration of promotions was significantly associated with percentage lower-fat food sales. CONCLUSIONS: Collaborative efforts among students, school food-service staff and research staff can be successful in implementing a large number of nutrition-related, school-wide promotional activities. These efforts can increase the sales of lower-fat foods in à la carte areas of school cafeterias. 相似文献
CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. Some CD9 cDNA have been cloned in mammals and certain fish genera in recent years, but goat and sheep counterparts of cattle, human and mouse have not been identified. To facilitate the studies, we cloned the cDNA encoding for CD9 of cashmere goat (Capra hircus) and sheep (Ovis aries), and expressed sheep CD9 in Escherichia coli cells. Structural analysis indicated for both goat and sheep that a 1123 bp cDNA spanned an open reading frame of 681 bp which predicted a protein of 226 amino acids with a typical TM4SF structure, including four highly conserved transmembrane domains, two extracellular domains and a CCG motif, which is a hallmark of the TM4SF. The predicted amino acid sequences were highly homologous to those of cattle, mouse and human CD9. Molecular phylogenetic analysis based on CD9 cDNA sequences indicated that goat and sheep CD9 were closely related to CD9 of cattle, which is in agreement with their morphological taxonomy. 相似文献
Ketamine is a rapid acting, potent, nonspecific, noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist commonly used for inducing general anesthesia and for providing post-operative pain management and may possibly lessen the need for other potentially harmful or contraindicated analgesics in camelids, such as nonsteroidal anti-inflammatory drugs. Prior to determining the effectiveness of CRI ketamine for analgesia, a safe, sub-anesthetic dose was established that did not produce untoward side effects, sedation or alter normal behavior. Six healthy male alpacas (40–90 kg) were used for the trial and each acted as its own control. Each alpaca was randomly assigned to receive ketamine at 20 and 40 μg kg–1 minute–1 in 500 mL saline. A blinded observer recorded heart rate, respiratory rate, and body temperature hourly, and behavior for 8 hours. There was a 72-hour washout period between each dosing regime. An equal volume saline CRI without ketamine was used as a control. Each alpaca was allowed a one-week washout prior to being anesthetized with isoflurane using mask induction. After achieving a stable plane of anesthesia, the MAC value for isoflurane was determined. Ketamine was infused at 40 μg kg–1pre-existing pain is unknown, but for elective procedures, preemptive analgesia using ketamine CRI in alpacas may be beneficial. 相似文献
The variation in milk acetone concentration between cows and the resumption of ovarian activity was determined in 68 Holstein–Friesian cows grazing pasture. Cows calved in two groups approximately three weeks apart and were assessed over the first 60 d of lactation.
The largest coefficient of variation (152%) for milk acetone concentration between cows was found in the first week of lactation. This variability in milk acetone concentration was largely explained by a consistent cyclic pattern, in relation to the day of the study, with regular peaks at 21–26 d intervals. These peaks were synchronised between cows, and cows in the second group synchronised with cows in the first group.
Milk acetone peaks greater than 0.4 mmol/L were associated with a significant drop in milk yield, possibly due to energy deprivation around this time. In this respect, liveweight change over the first 60 d of lactation was significantly negatively correlated (r = − 0.29, p < 0.05) with the mean milk acetone concentration taken over the same period. However, there was no significant relationship (p > 0.05) between mean or maximal milk acetone concentration and the resumption of ovarian activity for the cows that resumed ovarian activity in the first 9-weeks post-partum.
The results of this study suggest that the use of milk acetone as a practical indicator of energy balance is limited due to its variability over time. Milk acetone concentration may be useful in experimental situations or when regular milk samples can be obtained in the field, such as in-line analysis. There is a need to undertake more research to elucidate the implications of the cyclic pattern of acetone concentration in milk on reproductive performance. 相似文献
The objective of this study was to quantify the differences in the nutritive value over 4 seasons, of 7 C3 temperate grasses, 2 C4 tropical grasses and 11 clover species used as forages for dairy cows. The nutritive value was assessed in terms of nutrient content and the availability of effective rumen degradable protein, rumen by pass protein, metabolisable protein (MP) and fermentable metabolisable energy.
All species were grown in plots as monocultures under conditions of non limiting nutrients and moisture and harvested by mechanical means. All species had a high crude protein content and this resulted in a high effective rumen degradable protein: fermentable metabolisable energy ratio varying from 15, for cowpeas (Vigna unguiculata) to 29 for birdsfoot clover (Lotus corniculatus), and all were above the ratio of 11 required for optimal microbial protein synthesis in the rumen of dairy cows. The calculated availability of MP varied from 105 g/kg dry matter (DM) for cowpeas to 173 g/kg DM for berseem clover (Trifolium alexandrinum) indicating that all forages would be able to meet the requirements of dairy cows producing up to 30 L/milk/day, provided they were able to consume over 19 kg DM of forage/cow/day.
Grasses had much higher hemicellulose (neutral detergent fibre minus acid detergent fibre) content than legumes. Kikuyu (Pennisetum clandestinum), a C4 grass, had a higher proportion of hemicellulose content than the C3 temperate grasses. Perennial ryegrass (Lolium perenne) and kikuyu had a similar metabolisable energy (ME) density (9.9 MJ/kg DM) in summer. The mean ME density of perennial ryegrass, prairie grass (Bromus wildinowii) and short rotation ryegrass (Lolium multiflorum) winter was similar at 10.6 MJ/kg DM and slightly higher than cocksfoot (Dactylus glomeratus), phalaris (Phalaris tuberosa) and fescue (Fescue arundunicea) which had a mean ME density of 10 MJ/kg DM.
All forages grown were able to satisfy MP and ME requirements of dairy cows producing up to 30 L milk/cow/day, provided they were able to consume sufficient forage to achieve this level of production. 相似文献
Automatic milking systems (AMS) present an opportunity for dairy farmers to not only improve their lifestyle and conditions of work, but also save on labour costs and/or increase the time available to focus on overall farm management. However, the viability of AMS will rely on achieving high levels of pasture utilization. Well‐established pasture management principles are implemented on many conventional milking system (CMS) farms and high levels of pasture utilization are achieved; however, the ability to follow these same principles on AMS is unknown. This study compared levels of pasture utilization and pre‐ and post‐grazing pasture mass between AMS and CMS farms at the same site when managed by the same pasture management principles. From 1 March 2007 to 29 February 2008, pre‐ and post‐grazing compressed height, milk yield and milk composition data were collected for two CMS farms and one AMS farm at the Elizabeth Macarthur Agricultural Institute, Camden, Australia. Despite differences in pre‐ and post‐ grazing pasture mass between milking systems, pre‐grazing mass was predominantly maintained within the bounds of 2200 and 2500 kg DM ha?1 and post‐grazing mass between 1400 and 1500 kg DM ha?1 (5–6 cm height). Similar levels of pasture utilization (mean 13 500 kg DM ha?1 year?1) were recorded between AMS and CMS farms. These findings highlight the ability to follow established grazing management principles and achieve high levels of pasture utilization on pasture‐based AMS farms. 相似文献
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