Databases tailored for biodiversity conservation     

Smith AT  Boitani L  Bibby C  Brackett D  Corsi F  da Fonseca GA  Gascon C  Dixon MG  Hilton-Taylor C  Mace G  Mittermeier RA  Rabinovich J  Richardson BJ  Rylands A  Stein B  Stuart S  Thomsen J  Wilson C 《Science (New York, N.Y.)》2000,290(5499):2073-2074

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A high-resolution root spatiotemporal map reveals dominant expression patterns     

Brady SM  Orlando DA  Lee JY  Wang JY  Koch J  Dinneny JR  Mace D  Ohler U  Benfey PN 《Science (New York, N.Y.)》2007,318(5851):801-806

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Reproducing in cities     

Mace R 《Science (New York, N.Y.)》2008,319(5864):764-766

Reproducing in cities has always been costly, leading to lower fertility (that is, lower birth rates) in urban than in rural areas. Historically, although cities provided job opportunities, initially residents incurred the penalty of higher infant mortality, but as mortality rates fell at the end of the 19th century, European birth rates began to plummet. Fertility decline in Africa only started recently and has been dramatic in some cities. Here it is argued that both historical and evolutionary demographers are interpreting fertility declines across the globe in terms of the relative costs of child rearing, which increase to allow children to outcompete their peers. Now largely free from the fear of early death, postindustrial societies may create an environment that generates runaway parental investment, which will continue to drive fertility ever lower.  相似文献   

Sustainable Fishery Systems     

Pamela M. Mace 《Fish and Fisheries》2002,3(1):54-55

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利用重组近交系群体检测花生青枯病抗性SSR标记   总被引：7，自引：1，他引：7  

姜慧芳  陈本银  任小平  廖伯寿  雷永  傅廷栋  马朝芝  E Mace  J H Crouch 《中国油料作物学报》2007,29(1):26-30

用抗青枯病花生品种远杂9102与感病品种Chico杂交，从F2起用单粒传法构建了花生重组近交系群体（RIL）F6和F7。采用354对SSR引物对重组近交系F6群体的基因组DNA鉴定，获得多态性标记45个。结合重组近交系群体F6和F7青枯病抗性鉴定结果，应用相关软件统计分析，构建了栽培种花生部分遗传连锁图。图谱总长度为603.9cM，含29个标记（28个SSR标记和1个表型标记）的8个连锁群，还有17个独立的SSR标记；获得了与青枯病抗性相关的SSR标记2个（7G02和PM137），位于该图谱的第1连锁群上，与青枯病抗性基因间的遗传距离为10.9cM和13.8cM，并且位于抗性基因的两侧，两标记间的距离为23.7cM。  相似文献   

Birth of Kids After Artificial Insemination with Sex‐Sorted,Frozen‐Thawed Goat Spermatozoa     

R Bathgate  N Mace  K Heasman  G Evans  WMC Maxwell  SP de Graaf 《Reproduction in domestic animals》2013,48(6):893-898

Successful sex‐sorting of goat spermatozoa and subsequent birth of pre‐sexed kids have yet to be reported. As such, a series of experiments were conducted to develop protocols for sperm‐sorting (using a modified flow cytometer, MoFlo SX^®) and cryopreservation of goat spermatozoa. Saanen goat spermatozoa (n = 2 males) were (i) collected into Salamon's or Tris catch media post‐sorting and (ii) frozen in Tris–citrate–glucose media supplemented with 5, 10 or 20% egg yolk in (iii) 0.25 ml pellets on dry ice or 0.25 ml straws in a controlled‐rate freezer. Post‐sort and post‐thaw sperm quality were assessed by motility (CASA), viability and acrosome integrity (PI/FITC‐PNA). Sex‐sorted goat spermatozoa frozen in pellets displayed significantly higher post‐thaw motility and viability than spermatozoa frozen in straws. Catch media and differing egg yolk concentration had no effect on the sperm parameters tested. The in vitro and in vivo fertility of sex‐sorted goat spermatozoa produced with this optimum protocol were then tested by means of a heterologous ova binding assay and intrauterine artificial insemination of Saanen goat does, respectively. Sex‐sorted goat spermatozoa bound to sheep ova zona pellucidae in similar numbers (p > 0.05) to non‐sorted goat spermatozoa, non‐sorted ram spermatozoa and sex‐sorted ram spermatozoa. Following intrauterine artificial insemination with sex‐sorted spermatozoa, 38% (5/13) of does kidded with 83% (3/5) of kids being of the expected sex. Does inseminated with non‐sorted spermatozoa achieved a 50% (3/6) kidding rate and a sex ratio of 3 : 1 (F : M). This study demonstrates for the first time that goat spermatozoa can be sex‐sorted by flow cytometry, successfully frozen and used to produce pre‐sexed kids.  相似文献   

Molecular Cloning and Characterization of CD9 cDNA from Sheep and Cashmere Goat     

WJ Xing  LQ Wang  Q Wu  SC Ren  XH Bao  S Bou 《Reproduction in domestic animals》2010,45(3):383-392

CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. Some CD9 cDNA have been cloned in mammals and certain fish genera in recent years, but goat and sheep counterparts of cattle, human and mouse have not been identified. To facilitate the studies, we cloned the cDNA encoding for CD9 of cashmere goat (Capra hircus) and sheep (Ovis aries), and expressed sheep CD9 in Escherichia coli cells. Structural analysis indicated for both goat and sheep that a 1123 bp cDNA spanned an open reading frame of 681 bp which predicted a protein of 226 amino acids with a typical TM4SF structure, including four highly conserved transmembrane domains, two extracellular domains and a CCG motif, which is a hallmark of the TM4SF. The predicted amino acid sequences were highly homologous to those of cattle, mouse and human CD9. Molecular phylogenetic analysis based on CD9 cDNA sequences indicated that goat and sheep CD9 were closely related to CD9 of cattle, which is in agreement with their morphological taxonomy.  相似文献   

Early Meiotic‐Specific Protein Expression in Post‐natal Rat Ovaries     

P Zhang  LX Lv  WJ Xing 《Reproduction in domestic animals》2010,45(6):e447-e453

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What is your diagnosis? Hypoadrenocorticism (Addison's disease)     

Mace NR 《The Journal of small animal practice》2002,43(2):57, 91-57, 92

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治疗奶牛乳腺炎的新药盐酸吡利霉素研究进展   总被引：1，自引：0，他引：1  

徐福亮  申维金 《中国兽药杂志》2007,41(12):38-41

综述了治疗奶牛乳腺炎的新药——盐酸吡利霉素最新研究进展.对其基本药学性质、药动、药效、毒性和残留等多方面进行了详细阐述.  相似文献