O型口蹄疫病毒结构蛋白VP1的原核表达与抗原性分析     

徐程  陈亚波  方维焕 《动物医学进展》2007,28(6):14-18

研究分析了O型口蹄疫病毒(FMDV)结构蛋白VP1与当前猪FMDV疫苗血清的免疫反应性.将VP1基因克隆至原核表达载体pET32c,并在大肠埃希菌BL21中得到了表达,Western blot分析表明该重组蛋白与豚鼠O型FMDV标准阳性血清具有良好免疫反应性.目的蛋白经纯化后用ELISA分析其与猪疫苗血清的免疫反应性,结果显示该重组VP1蛋白(rVP1)只能与部分O型FMDV疫苗血清反应.推测当前使用的不同O型FMDV疫苗毒株在VP1重要中和抗原位点G-H环(134 aa～158 aa)与C末端(200 aa～213 aa)存在较大差异.  相似文献   

牦牛输卵管上皮细胞和卵泡颗粒细胞的培养     

郭宪  阎萍  许保增  曾玉峰  裴杰 《动物医学进展》2007,28(8):29-32

分离培养牦牛输卵管上皮细胞和卵泡颗粒细胞的目的是克服体外受精中早期胚胎发育阻断,建立有利于牦牛早期胚胎体外发育的共培养体系.采集牦牛输卵管上皮细胞进行原代及传代培养,同时从卵泡液中获取颗粒细胞进行原代培养,培养过程中观察2种细胞的生长方式和形态特点.输卵管上皮细胞贴壁生长时,呈多边形,且呈单层成簇生长.培养144 h～216 h,可形成细胞单层.颗粒细胞贴壁生长时,呈现聚集生长特性,贴壁细胞形状不规则,呈放射状.培养72 h～96 h,形成颗粒细胞单层.  相似文献   

H5N1亚型禽流感灭活疫苗免疫鸵鸟后抗体消长规律及免疫程序的研究     

刘佩红  周锦萍  瞿浩生  刘明华  倪惠军  金笑敏  卢军  张维谊  徐新红 《动物医学进展》2007,28(Z1):1-4

为了探讨鸵鸟对H5亚型禽流感灭活疫苗的免疫原性,做好鸵鸟禽流感的防控工作,采用哈尔滨兽医研究所研制的重组H5N1亚型禽流感疫苗,不同剂量免疫鸵鸟,用HI方法检测母源抗体和免疫抗体,根据母源抗体的衰减和免疫抗体的消长规律确定首免和再免日龄.结果表明,雏鸵鸟母源抗体能维持约3周～8周;8周龄时分组首免,C1组产生的免疫反应优于C2组,抗体峰值能达到7.50 log2,维持时间为9周左右;17周龄时C1组以3.0 mL/羽进行二免,2周后抗体达到7.40log2,3周～7周抗体维持在高峰值,最高达8.80log2,以后逐渐下降,有效抗体水平能维持至接种后25周左右.二免后25周进行三免,以5 mL/羽三免后2周抗体可达到9.80log2,2周～4周抗体维持在最高峰,以后缓慢下降,期间抗体时有起伏,但有效抗体水平约可维持1年时间.三免后45周内抗体水平合格率均在70%以上.根据抗体消长规律,初步推荐了鸵鸟的免疫程序.  相似文献   

食蟹猴骨髓采集穿刺方法及其骨髓象测定     

徐龙建  唐贤丽 《动物医学进展》2007,28(Z1):15-17

为探索采集食蟹猴骨髓的操作方法及其正常骨髓象指标,试验采集了60只食蟹猴的髂骨骨髓,并测定其骨髓象、测得的骨髓象及采集过程中的注意事项.结果表明,可以顺利的采出骨髓,雌雄之间晚幼红细胞、粒红比有显著性差异(P＜0.05),其余无显著差异.  相似文献   

叶面喷施硼酸对苹果果实硼和钙含量的影响   总被引：2，自引：0，他引：2  

魏宗梅  许雪峰  李天忠  王忆  孔瑾  韩振海 《园艺学报》2007,34(5):1111-1116

 以‘富士’苹果为试材，通过幼果期、果实膨大期和果实成熟期叶面喷施硼酸，研究硼对果实硼和钙含量的影响。结果表明：从叶面喷硼对果实吸收硼的短期效果来看，果实发育的不同时期叶面喷施硼酸均促进果实对硼、钙的吸收，并且处理的效果顺序是：幼果期>果实膨大期>果实成熟期；在果实采收时硼含量为：果实膨大期>果实成熟期>幼果期。不同时期叶面喷施硼酸均提高了果肉细胞中水溶态硼、半束缚态硼和束缚态硼的绝对含量，从3种形态硼所占的比例来看，随着果实不断发育，水溶态硼和半束缚态硼比例升高，而束缚态硼比例下降。  相似文献   

不同灵芝类群药效的特异性研究   总被引：4，自引：0，他引：4  

陈璐  罗霞  曾谨  许小燕  江南  郑林用 《中国食用菌》2007,26(6):40-43

通过对10种灵芝的表型性状及亲缘关系进行分析将灵芝聚为3类,对不同类群灵芝菌株的免疫调节、抗疲劳及改善睡眠的药效作用进行了比较研究。结果表明:灵芝的功效具有特异性,不同类群间存在功效差异性。  相似文献   

乙酰水杨酸调控猕猴桃果实后熟软化进程中的Ad-EXP1基因表达   总被引：1，自引：0，他引：1  

杨绍兰  陈妙金  张波  李鲜  徐昌杰  陈昆松 《果树学报》2007,24(6):778-782

以布鲁诺猕猴桃(Actinidia deliciosa cv.Bruno)成熟果实为材料,根据植物α-expansin(EXP)基因的氨基酸保守序列设计简并引物,利用RT-PCR方法结合3’RACE扩增得到1个长为957bp的cDNA片段(Ad-EXP1)。该基因片段编码211个氨基酸,与其它植物该基因核苷酸同源性为59%～85%,氨基酸同源性为73%～91%。Northern杂交结果表明,Ad-EXP1基因在采收当天的果实中表达很弱,随着果实成熟衰老进程加快趋于增强;乙酰水杨酸(ASA)处理延缓果实后熟软化和乙烯生成,也显著抑制Ad-EXP1基因表达。鉴于Ad-EXP1表达丰度与乙烯生成、果实软化程度的一致性,推测该基因在猕猴桃果实后熟软化进程中起着重要作用。  相似文献   

Effects of celecoxib on cardiac myocyte apoptosis after myocardial infarction     

XIA Yong  ZHANG Yong  LI Dong-ye  LIN Li  XU Rui-jin  YU Hao  DING Ru-yue  YANG Yu  CHEN Qing-zhi 《园艺学报》2007,23(1):49-52

AIM: To investigate the effects of celecoxib, a selective cyclooxygenase-2 inhibitor, on antioxidative capability and apoptosis of cardiac myocytes after myocardial infarction. METHODS: 24 New Zealand rabbits were divided into three groups randomly (8 in each group): sham-operated group (sham group), myocardial infarction group (MI group), celecoxib group (Cele group, 10 mg kg^-1·d^-1, qd, with the drugs gastric gavage for six weeks). The NO concentration, total antioxidative capability (T-AOC), the activity of constitutive nitric oxide synthase (cNOS) and inducible NOS (iNOS) in cardiac tissue homogenate, adjacent to the infracted area, were detected. The pathological changes were observed by light microscope and electron microscopy. The expressions of Bcl-2 and Bax protein in myocytes were observed using immunohistochemistry, and the degree of apoptosis were examined by TUNEL. RESULTS: Cardiac tissue in MI group presented interstitial edema, fibroplastic proliferation, inflammatory cellular infiltration, and vacuolar degeneration in cardiac myocytes. The results of electron microscopy showed that myocytes presented more changes caused by ischemic injury: widened interspace of myofibril, disordered myofibrillae, focal lysis of myofilament, ectasia of sarcoplasmic reticulum. In Cele group, the pathological changes were light, the NO^-₂/ NO^-₃ concentration, the activity of iNOS were lower (P<0.05), while the activity of cNOS and T-AOC were higher (P<0.05) than those in MI group. The expression rate of Bcl-2 protein in Cele group was higher than that in MI group, while the Bax was lower (P<0.01). The number of apoptotic myocytes was lower than that in MI group (P<0.01).CONCLUSION: Celecoxib decreases the number of apoptotic cardiomyocytes and increases the antioxidative capability after myocardial infarction.  相似文献   

Effect of uric acid on the maturation and the biological function of BMDCs     

MA Xiao-jun  TIAN De-ying  XU Dong  ZHU Hui-fen 《园艺学报》2007,23(1):95-98

AIM: To investigate the effect of uric acid on the maturation and the biological function of murine bone-marrow derived dendritic cells (BMDCs) in vitro.METHODS: BMDCs were cultured with GM-CSF, IL-4, LPS and uric acid. Cell phenotypes were analyzed by flow cytometry. MTT was used to detect the effect of uric acid on the function of BMDCs in stimulating the proliferation of T cells. IL-12 released by BMDCs was also detected. RESULTS: BMDCs were cultured and identified. Uric acid at concentrations of 200 mg/L and 400 mg/L increased the expression of the molecules (CD11c, CD83, CD86, IA/IE) on BMDCs surface and the IL-12 level in the culture supernatants (P<0.05), promoted the proliferation of T cells at the T: DC rate 5∶1, 10∶1, 20∶1 (P<0.05). However, uric acid at concentration of 70 mg/L had no effect on above molecule expression (P>0.05), no effect on T cell proliferation with BMDCs (P>0.05) was observed. CONCLUSION: Uric acid promotes the differentiation, maturation, the expression of co-stimulatory molecules, the IL-12 production in BMDCs and enhances the ability of BMDCs to stimulate the proliferation of T cell in a specical dose range.  相似文献   

Biological characteristics of JAK2 transduced CD34⁺ cells from cord blood during ex vivo expansion     

ZHAO Sheng-ming  GU Xi-chun  CHANG Nai-bai  XU Xiao-dong  PEI Lei 《园艺学报》2007,23(12):2460-2464

AIM: To explore the feasibility and biological characterization of long-term regulated expansion of JAK2 transduced human CD34⁺ cord blood cells in vitro.METHODS: A retrovirus (RV) vector which contains JAK2 catalytic domain and two binding sites for a chemical inducer,dimerization (AP20187),was cloned (designated MGI-F₂JAK2).CD34⁺cells were enriched from cord blood with a MiniMACS system.The purified CD34⁺ cells were transfected with supernatant from the retrovirus packaging cell line that expressed JAK2.Following transduction,cells were expanded into four groups: AP20187 alone,FL alone,TPO,alone,AP20187+FL+TPO,respectively.The expanded cells were monitored by GFP expression,immunophenotyping,progenitor colony assay,karyotype analysis as well as tumorigenesis in nude mice.RESULTS: The purity of selected CD34⁺ cells was over 91% and gene transfer rate was 49.32%±6.21%.Only the group of AP20187 +FL+ TPO was obtained a significant sustained outgrowth of the transduced CD34⁺ cord blood cells.The percentage of GFP+ cells consistently produced a rise to the 90% peak level by the end of 8th week of culture.Flow cytometry analysis showed that the phenotype of the expanded cells was CD33⁺,CD61⁺ and Gly-A⁺ partial positive;CD38⁺ and HLA-DR⁺ strong positive,while CD2,CD7 and CD19 were almost negative.Colony assays performed in methycelluos,which can give rise to BFU-E,CFU-GM and CFU-Mix,the CFU-GM was predominantly in all colonies.The tumor was not observed in nude mice and the karyotype analysis was normal from expanded cells.CONCLUSION: The results demonstrate that AP20187-mediated activation of JAK2 signaling is capable of stimulating expansion JAK2 transduced CB CD34⁺ cells in combination with FL and TPO.This system may have applications for studies in signaling transduction,hematopoiesis,and for gene and cell therapy.  相似文献