On the fracture morphology in wood     

M. Bariska  L. J. Kučera 《Wood Science and Technology》1985,19(1):19-34

Summary Failure forms caused by axial ultimate compression stress in three softwood and nine hardwood species and in model specimens made of wood, paper and plastics are described. Three categories of failure forms are distinguished: 1. Wood characteristic failure forms are connected with the general anisotropic structure of the wood. 2. Failure forms specific to the species are modifications of the first category arising from predominant anatomical structures. 3. Modifications of the failure forms are also induced experimentally. The interdependence among the anatomical structure, strength characteristics and failure forms of the wood specimens are examined by statistical methods. The structure cipher introduced in this paper, as a numerical characteristic of the anatomical features of the wood species, is seen to be the most important influencing factor as regards the intensity and pattern of the fracture, followed in second and third place by the geometry of the specimen and its specific gravity. Specimen volume and other factors are shown to have only a marginal influence on the fracture morphology.The strength tests were carried out as part of a diploma thesis by K. Buchmüller. Also the assistance of E. Risi in measuring and evaluating is gratefully acknowledged  相似文献   

非洲猪瘟病毒无标签p30-ELISA抗体检测方法的建立及应用   总被引：1，自引：1，他引：0  

于学祥  陈晓雨  李栋凡  孙琪  库旭钢  范盛先  杨汉春  何启盖 《畜牧兽医学报》2022,53(5):1517-1526

非洲猪瘟(African swine fever, ASF)是由非洲猪瘟病毒(African swine fever virus, ASFV)引起猪的一种急性、热性、出血性、高度接触性传染病,临床症状以败血症、皮炎和关节炎为特征,高发病率和高死亡率。为建立临床检测ASFV抗体的间接ELISA检测方法,本研究扩增了ASFV-CP204L基因,通过pET-30a原核表达系统表达p30蛋白,使用Ni-NTA纯化表达产物,通过肠激酶切除外源性蛋白,得到无His-组氨酸标签的p30蛋白,以此为诊断抗原,建立间接ELISA方法。结果显示：表达的无标签p30重组蛋白大小约为30 ku,与ASF阳性猪血清具有较好的反应原性;确定ELISA抗原包被浓度为1 μg·mL^-1,根据ROC曲线下面积确定S/P值>0.398判定为阳性,批内、批间变异系数均<10%;与PCV2、CSFV、PRV-gE、PRRSV阳性血清无交叉反应与INGENASA商品化试剂盒总符合率为97.78%。用该方法分别检测标准阳性血清、动物感染试验血清和收集的区域性临床血清644份,该方法最低可检测到1∶512倍稀释的标准阳性血清样品;检测感染动物血清,其中80%(4/5)的试验动物在第10天抗体为阳性。644份临床猪血清样品中抗体阳性率为7.61%,其中,母猪、后备母猪、仔猪、保育猪和育肥猪抗体阳性率分别为3.03%、0%、4.94%、7.55%和28.7%。本试验建立的ASFV-p30间接ELISA方法具有良好的特异性、灵敏度和重复性,可应用于ASFV的抗体检测,为ASF的诊断和流行病学调查提供了技术手段。  相似文献   

Soft Tissue Foreign Body Removal Technique Using Portable Ultrasonography     

Karolina Paziana  J. Matthew Fields  Masashi Rotte  Arthur Au  Bon Ku 《The Forestry Chronicle》2012,23(4):343-348

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Genetic dissection of leaf area by jointing two F2 : 3 populations in maize (Zea Mays L.)     

Lixia Ku  Jun Zhang  Jia Chen Zhang  Shulei Guo  Haiying Liu  Ruifang Zhao  Quxia Yan  Yanhui Chen 《Plant Breeding》2012,131(5):591-599

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基于Mean-shift和提升小波变换的棉花叶片边缘的图像检测   总被引：2，自引：2，他引：0  

李寒  王库  边昊一 《农业工程学报》2010,26(13):182-186

该文提出了一种基于Mean-shift和提升小波变换的具有复杂背景的棉花叶片边缘检测算法。该方法首先用Mean-shift算法对彩色图像进行平滑,然后对平滑后的图像进行提升小波变换,以将平滑后的图像进行灰度增强。最后基于Canny算子对图像进行边缘检测。该算法能有效减少非边缘噪声,并且能够有效提取相互重叠叶片的边缘。与传统边缘检测方法边缘检测结果进行对比,该方法能够更加鲁棒地提取复杂背景下的重叠叶片边缘,其有效性和准确性是很明显的。  相似文献   

有限元法在打结器钳嘴应力分析中的应用     

刘智强  张志毅  黄伟  李岩  布库  万其号 《农机化研究》2009,31(9)

将钳嘴PROE模型无缝导入ANSYS软件中,利用有限元方法对钳嘴易产生应力集中的部位进行了分析.通过计算得到了钳嘴的结构应力分布规律和变形量;通过对危险截面的强度校核验证了原设计方案的可靠性,从而为该钳嘴优化设计方案的最终确定提供了理论依据,同时也为轴类结构设计提供了一种有效的方法.  相似文献   

Glutathione Transferase Isozymes Involved in Insecticide Resistance of Diamondback Moth Larvae   总被引：1，自引：0，他引：1  

Ku C. C.  Chiang F. M.  Hsin C. Y.  Yao Y. E.  Sun C. N. 《Pesticide biochemistry and physiology》1994,50(3)

In addition to the three glutathione transferase (GST) isozymes already identified in diamondback moth larvae, Plutella xylostella (L.), a fourth one, GST-4, was purified from a teflubenzuron (TFB)-resistant strain. This GST isozyme was similar to GST-3 in terms of biochemical and toxicological properties. GST-4, a homodimer with a subunit molecular mass of 26.6 kDa and a pI of ca. 8.9, displayed even stronger substrate preference than GST-3 for 1,2-dichloro-4-nitrobenzene and several organophosphorus insecticides, i.e., parathion, methyl parathion, and paraoxon. These two proteins were highly immunorelated and shared at least the first eight amino acids at the N-terminus. Immunoblotting analysis indicated that polyclonal antiserum raised against GST-3 cross-reacted with GST-1 and GST-2 at least 40-fold less intensely than with the antigen. Using this antiserum as probe, higher amounts and greater variations of GST-3/GST-4 were observed in larvae of a methyl parathion- and a TFB-resistant strain compared with a susceptible and a fenvalerate-resistant strain. Among the six lepidopterous insects examined, only Spodoptera exigua larvae clearly had proteins immunorelated to GST-3/GST-4 of diamondback moth. No such cross-reactivity was observed in Musca domestica, Drosophila melanogaster, and Aedes aegypti.  相似文献   

An immunohistochemical study of the gastrointestinal endocrine cells in the C57BL/6 mice   总被引：12，自引：0，他引：12  

Ku SK  Lee HS  Lee JH 《Anatomia, histologia, embryologia》2003,32(1):21-28

The regional distributions and relative frequencies of some gastrointestinal endocrine cells in the eight portions (fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum) of the gastrointestinal tract of C57BL/6 mouse was studied with immunohistochemical method using seven types of specific anti-sera against chromogranin A (CGA), serotonin, somatostatin, human pancreatic polypeptide (HPP), glucagon, gastrin and cholecystokinin (CCK)-8. In this study, all these seven types of immunoreactive (IR) cells were identified. Most of these IR cells in the intestinal portion were generally spherical or spindle in shape (open-type cell) while cells showing round in shape (closed-type cell) were found in the intestinal gland and stomach regions occasionally. Their relative frequencies were varied according to each portion of gastrointestinal tract. CGA-IR cells were demonstrated throughout the whole gastrointestinal tract and they showed most predominant in the pylorus and duodenum. Serotonin-IR cells were detected throughout whole gastrointestinal tract and they showed highest frequency in the stomach and colon. Somatostatin-IR cells were demonstrated throughout whole gastrointestinal tract except for large intestine and showed highest frequency in the fundus. HPP-IR cells were found in the fundus with rare frequency. Peculiarly, glucagon-IR cells were restricted to the fundus, ileum and colon with a few frequencies. Gastrin-IR cells were restricted to the pylorus with numerous frequency and CCK-8-IR cells were observed in the pylorus, duodenum and jejunum with numerous and/or a few frequencies, respectively. In conclusion, some peculiar distributional patterns of gastrointestinal endocrine cells were found in C57BL/6 mouse.  相似文献   

Field trials on the prophylaxis of intramammary infections in pregnant heifers     

Malinowski E  Kłossowska A  Kaczmarowski M  Kuźma K  Markiewicz H 《Polish journal of veterinary sciences》2003,6(2):117-124

The study was carried out in 5 farms on 174 pregnant heifers. Clinical examination of the udder and bacteriological tests of quarter secretion were performed between the 8th and 3rd week before parturition, and then the animals were divided into a control group (64 heifers) and 3 experimental groups and immediately treated. A group of 32 experimental heifers was injected once with antioxidants (Vitamin A--600,000 i.u.; Vitamin D3--200,000 i.u.; Vitamin E--1.5 mg/kg b.w., Selenium--0.022 mg/kg b.w., i.m.). The next group (26 heads) was intramammary infused with antibiotic DC product (cloxacillin). Heifers from last experimental group (52) were injected with lysosyme dimer in a single dose of 0.02 mg/kg b.w. Clinical and bacteriological examinations were made during the first week after calving. The presence of bacteria was found in secretion of 22.6-38.9% udder quarters in 56.2-71.2% of pregnant heifers. The number of infected quarters (cows) did not change distinctly in the first week after calving except the lysozyme dimer group, where a decrease by 30% was noted. The percentage of quarters with elevated somatic cell count was higher in antibiotic DC group and closely similar in the other groups. None of examined methods showed an acceptable prophylactic effect. Clinical mastitis cases during first week after parturition were mostly caused by Escherichia coli, Staph. chromogenes, Staph. simulans, Staph. aureus, Staph. hyicus, Str. uberis, Str. acidominimus and Enterococcus faecalis.  相似文献   

不同改良措施的研究综述     

刘库  谢应忠 《中国草地》2005,27(5):53-57

由于自然因素及人为因素的共同作用,使得我国植被退化日趋严重.综合前人不同的研究成果,探讨了不同改良措施对退化植被的影响。  相似文献