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991.
WEI Chang-kong YE Ren-gao LI You-ji YANG Nian-sheng DONG Xiu-qing YU Xue-qing 《园艺学报》2005,21(9):1669-1674
AIM: To investigate the association of gene polymorphism at position 196 of tumor necrosis factor receptor Ⅱ (TNFRⅡ) with systemic lupus erythematosus (SLE) in Chinese, and establish recombinant retroviral vector to analyze the function of the TNFRⅡ 196M/R. METHODS: The genotype at position 196 of TNFRⅡ was determined by PCR-RFLP in 106 SLE patients and 119 healthy controls in china. Human TNFRⅡ196M cDNA were amplified by PCR and cloned into PMD18-T vector. Then, PMD18-TNFRⅡ196R was induced by site-directed mutagenesis. The recombinant T vector, PMD18-TNFRⅡ196M and PMD18-TNFRⅡ196R, were subcloned into retroviral vector PLXSN. Both normal and variant were transfected into rat mesangial cell. The effects of TNFα on production of sTNFRⅡ and IL-6 were study by ELISA. RESULTS: (1) The frequency of TNFRⅡ196R allele was significantly higher than those in controls (35.2% vs 14.3%, P<0.05); (2) The recombinant retroviral vector (PLXSN-TNFR 196M and PLXSN-TNFR 196R) was constructed successfully; (3) rhTNFα caused a significant increase in IL-6 production by rat mesangial cells transfected with PLXSN-TNFRⅡ196R than that with TNFRⅡ196M. CONCLUSION: These data indicate that TNFRⅡ196R allele is associated with SLE in the Chinese. TNFRⅡ196R transduces the signals of TNFα more effectively than TNFRⅡ196M, which may be involved in the pathogenesis of SLE. 相似文献
992.
AIM: In this study, we investigated the anticancer effect and mechanisms of manumycin on pancreatic cancer cell line-Panc-1 and the role of p38MAPK pathway in apoptosis. METHODS: The test of anticancer effect was performed by MTT assay. Apoptosis was induced in the cells by manumycin and then treated with SB203580, a specific p38MAPK inhibitor. A quantitative caspase-3 activity assay kit was used in this experiment. RESULTS: Manumycin (6 μmol/L, 18 μmol/L, 54 μmol/L) significantly inhibited cell growth of pancreatic cancer cell line Panc-1. The inhibition rates 24 h after treatment with 6 μmol/L, 18 μmol/L and 54 μmol/L manumycin were 8.9%, 21.9% and 67.0%, respectively. Compared with the control group, the survival levels of the last two groups were of significant statistical difference (P<0.01). The anticancer effects also showed dosage-effect relationship, the value of IC50 24 h after treatment was 34.7 μmol/L. In addition, this reagent simultaneously activated caspase-3 protein, which was partly blocked by p38MAPK specific inhibitor, SB203580. CONCLUSION: Manumycin exerted anticancer effect on Panc-1 cell line via inducing cell apoptosis, which was partly regulated by p38MAPK. 相似文献
993.
包膜控释尿素对大棚番茄的增产与品质提高效应 总被引:4,自引:0,他引:4
对施用包膜控释尿素和普通尿素的大棚番茄进行了田间生产条件下产量与品质对比分析试验。结果表明:与普通尿素相比施用控释尿素有显著的增产效果。D 60控释处理(控释期60 d)产量最高,为68.85 t/hm2,比对照增产19.39 t/hm2,增产率为39.2%;比尿素增产10.10 t/hm2,增产率为17.2%;D 90(控释期90 d)比对照增产15.37 t/hm2,增产率为31.0%;比尿素增产6.08 t/hm2。增产率为10.3%;D 90与D 60两种控释尿素之间产量差异不显著。控释尿素具有增加单株果数、单果重的效果。D 90与D 60氮素当季利用率比普通尿素分别高65.5%和108.8%。硝酸盐含量,以D 90为最低,仅比对照高4.4%,比普通尿素低56%;D 60比对照高15%,比尿素处理低51.6%;尿素处理比对照高137.3%。V c和氨基酸含量,D 90为最高,分别比对照高1.00倍和2.46倍;比普通尿素处理高22%和100%;D 60与尿素两处理V c含量差异不显著,但氨基酸总量前者却比后者高48%。可溶性糖含量以D 60较高,普通尿素为最低。综合品质指标为:D 90>D 60>U。说明控释尿素具有显著地增产效应和提高蔬菜品质的突出效应,特别在提高蔬菜的安全品质方面贡献最大。综合各项,在番茄生产中以选用控释期为60 d的包膜控释尿素为最好。 相似文献
994.
水肥因素对膜下滴灌棉花产量和棉株群体冠层结构的影响研究 总被引:19,自引:0,他引:19
以新陆早7号为试材,进行了不同水肥条件下膜下滴灌棉株群体冠层结构测定和产量结果分析。试验表明,合理水肥条件下的棉株群体叶面积指数(LAI)、叶倾角(MLA)和散射光透过率(TD),在盛花盛铃期分别为3.55~3.95、40°~48°和18.5%~28.1%。在一定水肥范围内,滴灌棉花产量随灌水量和施肥量的增加而增加,但超过一定阈值产量有下降趋势。适宜水肥条件下产量最高。 相似文献
995.
三唑酮种衣剂对小麦白粉菌侵染和非侵染位点硅等元素含量的影响 总被引:3,自引:0,他引:3
借助扫描电子显微镜-X射线能谱分析方法从超微结构和元素水平观测了三唑酮种衣剂包衣处理和空白对照的小麦幼苗上白粉病菌侵染及非侵染位点硅等元素含量的变化。结果表明,接种后20~36 h内三唑酮种衣剂处理的麦苗侵染位点细胞内元素硅含量由5.204%上升为42.064%,非侵染位点由5.821%上升为12.342%;空白对照中侵染位点元素硅含量由低于检测水平上升为17.388%,非侵染位点接种后36 h时为19.460%。在处理和空白对照的麦苗上侵染和非侵染位点均检测到了含量不等的磷、硫、氯等元素存在。 相似文献
996.
997.
林间释放平腹小蜂防治银杏大蚕蛾研究 总被引:4,自引:1,他引:4
笔者于1999-2000年在城口银杏大蚕蛾发生区释放用其卵繁育的平腹小蜂防治银杏大蚕蛾,结果表明:放蜂区虫卵寄生率明显提高,其校正寄生率为69.30%,是一种可利用的天敌昆虫。可在银杏大蚕蛾发生区内推广应用。 相似文献
998.
The antagonistic fungus Phaeotheca dimorphospora was tested as a biocontrol agent against the Dutch elm disease pathogens, Ophiostoma ulmi and O. novo-ulmi , on Ulmus americana seedlings in the glasshouse. Curative inoculation of seedlings with P. dimorphospora had no significant effect on disease development. Conditioning inoculation of seedlings with the antagonist protected them against O. ulmi but not against the more aggressive O. novo-ulmi . In seedlings challenged with both the antagonist and either one of the pathogens, P. dimorphospora only spread locally around the inoculation point; however, the pathogens spread systemically throughout the whole plant. O. novo-ulmi survived in the region colonized by P. dimorphospora , whereas O. ulmi did not. 相似文献
999.
1000.
RAPD-PCR was used to characterize isolates of Rhizoctonia solani from bare patches in cereal and pasture crops at two locations in Western Australia, Newdegate and Esperance. All of the isolates belonged to anastomosis group 8, pectic zymogram group 1-1 or 2. The pectic zymogram assignment could be confirmed by RAPD-PCR. There was no difference in RAPD-PCR pattern between highly virulent and weakly virulent isolates at Newdegate, or between isolates from different patches at Newdegate. The Newdegate isolates were identical to isolates from Esperance, and to isolates from various locations in South Australia. 相似文献