Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody     

ZHANG Ling-ling  YUE Yao-jing  FENG Rui-lin  LI Hong-feng  GUO Ting-ting  YUAN Chao  NIU Chun-e  LIU Jian-bin  SUN Xiao-ping  HAN Ji-long  LIU Shan-bo  YANG Bo-hui 《中国畜牧兽医》2016,43(8):2156-2163

In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research.  相似文献   

浮游藻类与噬藻体生态功能及其互作关系研究进展     

王子艺  成亚辉  张仕颖  肖炜  段红平 《贵州农业科学》2020,48(11):114-120

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微生物菌群培养组学在动物医学中的应用     

彭娜  彭先启  乐敏 《畜牧兽医学报》2020,51(12):2942-2953

实验室条件下可培养的微生物约占自然界中微生物总数的1%，这限制了人们对99%未知微生物的认识和利用，而研究表明，那些“不可培养的微生物”是可以被开发和利用的，未能被纯培养的微生物才是未知微生物的主体。微生物培养组学探索利用多种培养条件和长时间的培养，结合基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF-MS）和16S核糖体RNA（rRNA）测序可以大规模鉴定各种微生物，同时利用全基因组测序和宏基因组测序手段对未知微生物进行深入分析。本文综述了国内外近年来微生物菌群培养组学在反刍动物胃肠道、禽类盲肠及家畜鼻腔微生物菌群研究中的最新进展，探讨将动物体内菌群培养组学方法应用于动物疾病防治领域的可行性。作为一个新兴的研究方法，尽管该培养组学还存在一些不够成熟的方面，但它的发展前景十分广阔，微生物菌群培养组学方法和其他研究方法的互补已经逐渐成为发展兽医微生物学新的突破口。  相似文献   

Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines     

Xiao-feng YUE  Yan-lun JU  Zi-zhu TANG  Ya-meng ZHAO  Xu-liang JIAO  Zhen-wen ZHANG 《农业科学学报》2019,18(9):2052-2062

The effects of the severity and timing of leaf removal(LR) on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season. High-performance liquid chromatography(HPLC) was used to analyze the amino acids profiles of grape berries and wines. The basal leaves were removed at three time points(40, 56 and 72 days after flowering, named LR40, LR56 and LR72, respectively) at two severity levels(one at which the first, third, and fifth basal leaves of each shoot were removed(50% level); and another at which the first six basal leaves were removed(100% level)). The results showed that leaf removal had little impact on total soluble solids(°Brix), titratable acidity, pH or berry weight. The LR72-50% treated grapes had higher berry weight, titratable acidity and °Brix than those of the other treatments. The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50% treated grapes(2 952.58 and 2 764.36 mg L~(-1), respectively); the lowest were detected in LR72-100% treated grapes(2 172.82 and 2 038.71 mg L~(-1), respectively). LR72-50% treatment significantly promoted the synthesis of aspartic acid, serine, arginine, alanine, aminobutyric acid and proline at both severity levels for grapes, the concentrations of all of these amino acids were increased relative to the control concentrations. The LR72-50%, LR40-100% and LR72-100% treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids, such as arginine, alanine and serine, than did the control wines. Of all the amino acids studied, glycine, tyrosine, cysteine, methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines. The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines.  相似文献   

双免耕覆盖对旱地土壤微生物、酶活性和冬小麦干物质转运的影响     

邵运辉  李俊红  岳俊芹  丁志强  张德奇  方保停  杨程  李向东 《麦类作物学报》2020,40(12):1486-1492

为了解双免耕覆盖下旱地土壤微生物数量、土壤酶活性及冬小麦干物质转运特征，研究了双免耕覆盖下麦田土壤中细菌、真菌、放线菌的数量、土壤蛋白酶和脲酶的活性、冬小麦花前和花后干物质向籽粒的转运量与传统耕作（对照）的差异。结果表明，与对照相比，双免耕覆盖下土壤中微生物的数量和酶活性在冬小麦不同生育时期和不同土层均显著（P<0.05）或极显著（P<0.01）高于对照；在0～60 cm土壤中，双免耕覆盖下微生物总量平均较对照高68.2%，差异达极显著水平；土壤中蛋白酶和脲酶活性平均较对照高 27.3%和32.7%。双免耕覆盖下冬小麦花后干物质积累量对籽粒产量的贡献率为44.2%，较对照增加了 61.7%；产量较对照提高18.4%，二者差异达极显著水平。  相似文献   

长期定位施肥对潮土磷素下移及有效磷生态阈值的研究   总被引：2，自引：0，他引：2  

张珂珂  黄绍敏  郭斗斗  宋晓  岳克  张水清  黄晨晨 《中国土壤与肥料》2020,(5):64-69

研究长期施肥条件下磷素下移情况及其与0～20 cm土层有效磷含量的关系,明确有效磷生态阈值对作物产量和农业生态安全有重要的意义。试验基于"国家潮土土壤肥力与肥料效益长期监测站" 30年定位试验,选取5个处理,即不施磷肥(NK)、施用氮磷钾化肥(NPK)、氮磷钾化肥和有机肥配施(MNPK)、MNPK处理施肥量的1.5倍(1.5NPKM)、氮磷钾化肥与玉米秸秆还田配施(SNPK),采集0～20、 20～40、40～60、60～80、80～100 cm土壤样品,分析有效磷含量,研究土壤有效磷垂直变化,并分析0～20 cm土层有效磷含量与磷素下移关系。结果表明,长期施用磷肥可提高有效磷含量,施磷量越高,有效磷含量增加越快;0～20 cm土层有效磷含量15.7～24.7 mg/kg,磷素下移慢,可下移到40～60 cm土层;而有效磷含量大于55.6 mg/kg时,磷素下移速度显著增加,下移到60～80 cm土层,并且下移量更大。综上,磷素下移的深度受耕层有效磷含量的影响,潮土区土壤有效磷生态阈值为25 mg/kg,既满足作物高产对养分的需要,又不对生态环境造成危害。0～20 cm土层有效磷含量为15～25 mg/kg时,控制磷的投入量;当0～20 cm土层有效磷含量超出25 mg/kg,减少磷的投入。  相似文献   

白花蛇舌草超微粉对小鼠免疫功能的影响     

王航  汤承  岳华 《中国畜牧兽医》2014,41(3):166-169

The assay was aimed to study the effect of Hedyotis diffusa Willd superfines powder on immune function. Three groups of mice were gavaged with the drugs at doses of 1.75,1.25 and 0.75 g/kg once a day for 7 consecutive days, repectively. The mice of control group were administered with the same volume of saline.At the last time, mice were injected into the muscle with Newcastle disease vaccine,and get peripheral blood on days 7, 14 and 21 after the medicine administration. The enhancement of innate immune responses were evaluated by using CCK-8 method, hemagglutination inhibition test, macrophage phagocytic function test and organ coefficient method. The levels of lymphocytes proliferative capacity, serum antibody, peritoneal macrophage phagocytosis and the indexes of immune organs in mice were extremely significantly enhanced (P＜0.01). Hedyotis diffusa dWilld superfines powder could extremely significantly regulate the immunity function of mice (P＜0.01).  相似文献   

浅析乌兰察布地区金叶榆嫁接以及嫁后管理技术     

徐翔宇  于泓民  马妍  常峥  岳颂民 《内蒙古林业调查设计》2015,38(1):64-65,69

金叶榆叶片金黄色,枝条密生,树冠丰满,生长旺盛,适合做多种造型,在园林绿化上应用较多;再者,抗寒、抗旱能力强,很适合在乌兰察布地区推广应用,为了满足绿化的需要,对该地区嫁接技术以及嫁后管理技术进行了总结,为当地金叶榆嫁接技术以及嫁后管理技术提供理论参考。  相似文献   

樱桃根腐病诱发因素研究     

岳文秀  蒋平 《耕作与栽培》2020,40(4):37-38

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基于双向电泳技术研究湖羊初乳和常乳乳蛋白质组差异     

翁秀秀  刘欣欣  乐祥鹏  李万宏  李发弟 《中国畜牧兽医》2021,48(3):865-872

试验旨在分析绵羊初乳和常乳乳蛋白质组的差异，揭示绵羊不同泌乳阶段的乳蛋白质组特征。选用6只胎次相同、预产期相近的湖羊母羊，分别采集分娩后第1、3、7天的初乳和第14、28、56天的常乳，每个时间点采集6份奶样并等体积混合，离心弃去乳脂肪，采用双向电泳（2-DE）技术对初乳和常乳的脱脂乳蛋白进行比较分析。结果发现，初乳中乳蛋白含量较高，且随着泌乳期的延伸，乳蛋白含量降低。以第1天乳蛋白2-DE图谱为参照，第3天检测到38个差异蛋白点，其中有20个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第3天图谱中，其余17个蛋白点呈现表达量的差异；第7天检测到35个差异蛋白点，其中有19个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第7天图谱中，其余15个蛋白点呈现表达量的差异；第14天检测到34个差异蛋白点，其中有11个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第14天图谱中，其余22个蛋白点呈现表达量的差异；第28天检测到38个差异蛋白点，其中有28个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第28天图谱中，其余9个蛋白点呈现表达量的差异；第56天检测到36个差异蛋白点，其中有26个蛋白点仅存在于第1天图谱中，有1个蛋白点仅存在于第56天图谱中，其余9个蛋白点呈现表达量的差异。综上，共检测出44个差异表达的蛋白点，其中有8个蛋白点仅存在于第1天图谱中，而这些蛋白主要涉及免疫活性功能。  相似文献