Genetic identification and morphology of naturally spawned eggs of the Japanese eel Anguilla japonica collected in the western North Pacific     

Tatsuki Yoshinaga  Michael J. Miller  Kazuki Yokouchi  Tsuguo Otake  Shingo Kimura  Jun Aoyama  Shun Watanabe  Akira Shinoda  Machiko Oya  Sachie Miyazaki  Kei Zenimoto  Ryusuke Sudo  Tetsuya Takahashi  Hyojin Ahn  Ryotaro Manabe  Seishi Hagihara  Hiroshi Morioka  Hikaru Itakura  Masamichi Machida  Kazuyuki Ban  Mayu Shiozaki  Bunpei Ai  Katsumi Tsukamoto 《Fisheries Science》2011,77(6):983-992

Eggs of the Japanese eel Anguilla japonica collected in the western North Pacific were identified by onboard species-specific polymerase chain reaction (PCR) and DNA nucleotide sequencing after the cruise. Fish eggs of various species were collected by large plankton net tows at 12 stations along the southern part of the West Mariana Ridge on 19–25 May 2009. A total of 43 fish eggs were distinguished morphologically as possibly being of A. japonica. Thirty-one of those were analyzed by PCR, which included 15 eggs collected at 12°50–55′N, 141°15–20′E (in 5 tows) that showed positive results. The 16S ribosomal RNA (rRNA) gene sequences of eggs determined after the cruise indicated that 31 A. japonica eggs had been collected. The remaining eggs were of mesopelagic eel species (Serrivomeridae and Derichthyidae), or unidentified species. The morphological characteristics of the A. japonica eggs were consistent with those of artificially spawned eggs, except they had a slightly larger diameter. The egg diameter range did not overlap with those of mesopelagic eels of the Serrivomeridae, which often spawn in the same area as A. japonica. These results suggest that egg diameter and embryo shape can be used to morphologically identify naturally spawned A. japonica eggs.  相似文献   

Developmental changes in drinking rate and ion and water permeability during early life stages of euryhaline tilapia, Oreochromis mossambicus, reared in fresh water and seawater     

H. Miyazaki  T. Kaneko  S. Hasegawa  T. Hirano 《Fish physiology and biochemistry》1998,18(3):277-284

Euryhaline tilapia (Oreochromis mossambicus) can breed either in fresh water (FW) or in seawater, and the developing embryos and larvae withstand direct transfer from FW to SW or vice versa, before the development of osmoregulatory organs. In the study, developmental changes in drinking rate and ion and water permeability were examined after transfer of the embryos from FW to SW. Drinking was measured by accumulation of fluorescent beads in the intestine and also by 14C-dextran accumulation he whole body. The drinking rate increased steadily from 2 to 10 days after hatching, and the larvae in SW consistently imbibed more water than those in FW. The diffusional water permeability remained low during embryonic stages but increased markedly after hatching in both FW and SW; the water permeability was consistently less in SW-adapted embryos and larvae than those in FW. In contrast, the turnover rates of chloride ion in SW were 50 to 100 times greater than those in FW, and increased markedly after hatching. The drinking rate as well as ion and water permeability of the tilapia embryos and larvae in FW and SW were comparable with those reported for stenohaline species. These results clearly indicate that different water and ion regulatory mechanisms are operating in the tilapia embryos and larvae in FW and those in SW to convey their strong euryhalinity.  相似文献   

Predicting unsaturated hydraulic conductivity of soil based on some basic soil properties     

J. Zhuang  K. Nakayama  G. R. Yu  T. Miyazaki 《Soil & Tillage Research》2001,59(3-4):143-154

Soil hydraulic conductivity is a crucial parameter in modeling flow process in soils and deciding water management. In this study, by combining the non-similar media concept (NSMC) to the one-parameter model of Brooks and Corey, a new NSMC-based model for estimating unsaturated hydraulic conductivity of various soils was presented. The main inputs are soil bulk density, particle-size distribution, soil water retention characteristic and saturated hydraulic conductivity of soil. The results indicated that the NSMC-based model could generally more accurately predict unsaturated hydraulic conductivity of soils, as compared to four one-parameter models and van Genuchten–Mualem model. This study, by introducing NSMC, provided a new way to incorporate soil physical heterogeneity into soil hydraulic simulation, and hence NSMC-based approach is expected to improve efficiency of the existing models in the simulation of soil water flow.  相似文献   

Population genetic structure and variability of Pacific herring <Emphasis Type="Italic">Clupea pallasii</Emphasis> in the stocking area along the Pacific coast of northern Japan     

Takuma Sugaya  Michihiro Sato  Emi Yokoyama  Yuta Nemoto  Tomonari Fujita  Hiroyuki Okouchi  Katsuyuki Hamasaki  Shuichi Kitada 《Fisheries Science》2008,74(3):579-588

ABSTRACT:   The genetic diversity of wild and hatchery-released Pacific herring Clupea pallasii collected from three brackish lakes and two bays in Honshu and Hokkaido, Japan was examined with five microsatellite loci. All loci showed high genetic variability with expected heterozygosities ranging from 0.815 to 0.945. Significant differences in genotypic and allelic distributions were detected among all locations except for between the two bays in Honshu Island. Pairwise population analysis based on the F _ST values showed close genetic relationships among the locations in Hokkaido Island, and the hierarchical analyses of molecular variance showed significant genetic difference between the two islands. Those results suggest the existence of subpopulations due to natal homing. In addition, stocked fish showed as much genetic diversity as the wild fish. The pairwise population analyses also showed close relationships between the hatchery fish and the wild fish in respective stocking areas, showing that no effects of stocking programs on genetic diversity of wild populations were detected.  相似文献   

Motility and fertility of cryopreserved spermatozoa of the Japanese sea cucumber Apostichopus japonicus     

Yuta Mizuno  Atsushi Fujiwara  Keisuke Yamano  Hiromi Ohta 《Aquaculture Research》2019,50(1):106-115

We developed both a cryopreservation method for Japanese sea cucumber spermatozoa and an artificial fertilization method using post‐thaw spermatozoa. Twenty per cent dimethyl sulfoxide (DMSO), 16% foetal bovine serum, and 64% artificial seawater were suitable cryodiluent, and the diluent was pre‐cooled to 0°C. Semen was diluted with the solution and enclosed in a 250 μl straw, cooled to ?50°C at 10.4 ± 0.4°C/min, and immediately immersed in liquid nitrogen. Although this method showed the highest post‐thaw motility in all the conditions we examined, its post‐thaw motility was still less than approximately 15%. Artificial fertilization was carried out by adding post‐thaw semen with a cryodiluent to the oocytes. The fertilization rate of 200 oocytes/ml seawater increased with the amount of post‐thaw semen from 1 to 5 μl but showed a significant decrease at 25 μl. This decrease was considered to be due to DMSO in the cryodiluent, because the fertilization rate of the fresh semen decreased sharply when the DMSO concentration around the oocytes was 1.0% or more. Further improvement in increasing post‐thaw motility and lowering the cryoprotectant concentration is necessary for commercial‐scale artificial fertilization.  相似文献   

Effects of different water management practices on the dry matter production process and characteristics in NERICAs     

Mayumi Kikuta  Daigo Makihara  Naoya Arita  Akira Miyazaki  Yoshinori Yamamoto 《Plant Production Science》2013,16(2):168-179

ABSTRACT

In this study, we aimed to clarify the effects of different water management strategies on dry matter production and yield performance of New Rice for Africa (NERICA) varieties. Dry matter production of NERICA 1 and NERICA 5 was compared with that of Yumenohatamochi, a Japanese upland variety, and Hinohikari, a Japanese lowland variety under three water regimes, i.e. continuously flooded, supplemental irrigation, and non-irrigation (rainfed). Total carbohydrate content in the panicles under different watering regimes was more closely related to post-heading photosynthates than pre-heading reserve assimilates. Dry matter production during ripening tended to decrease under low soil water conditions, whereas the dry matter translocated from the leaf and stem to the panicle tended to increase. Consequently, the distribution ratio of post-heading photosynthates in the total carbohydrate content declined in response to the reduction in available soil moisture. These results indicate that the total carbohydrate content vary depending on the soil water conditions. In NERICAs, dry matter production during ripening was lower than that in Japanese varieties, indicating that their dependence on pre-heading reserve assimilates was greater. In particular, post-heading photosynthate content of NERICA 1 was strongly affected by the variation in water management in comparison with that of other varieties. The decrease in crop growth rate during ripening in NERICA 1 can be mainly attributed to the lower post-heading photosynthate content. Thus, the ability of NERICA 1 to assimilate carbon after heading was considered to be potentially low, which has to be improved to achieve higher yield.  相似文献   

Seasonal changes in cellular compartmentation and membrane permeability to sugars in relation to early or high temperature-induced watercore in ‘Orin’ apples     

H. Yamada  T. Okumura  Y. Miyazaki  S. Amano 《The Journal of Horticultural Science and Biotechnology》2013,88(6):1069-1073

Summary

The intensity of watercore in ‘Orin’ apples (Malus domestica Borkh.) from the outer canopy increased from early-July to mid-August, followed by a decrease through late-October, while no watercore was detected in inner canopy fruit during the same period. Seasonal changes in sorbitol content in each cellular compartment paralleled the development of watercore. Significantly higher sorbitol, fructose and glucose contents in the vacuoles of cells in outer canopy fruit than in inner canopy fruit suggest that active metabolism and uptake of sorbitol might occur in watercored apples in mid-August. The permeability of sorbitol across the tonoplast was significantly higher in outer than in inner canopy fruit after mid-August, whereas no difference was observed between bearing position for the other sugars or for the plasma membrane. These data suggest that elevated sorbitol levels in the intercellular spaces might result from active apoplastic unloading and not from an inhibition of metabolism and/or an increase in leakage from cells.  相似文献   

Infrared spectroscopic evidence for protonated water clusters forming nanoscale cages     

Miyazaki M  Fujii A  Ebata T  Mikami N 《Science (New York, N.Y.)》2004,304(5674):1134-1137

Size-dependent development of the hydrogen bond network structure in large sized clusters of protonated water, H+(H2O)n (n = 4 to 27), was probed by infrared spectroscopy of OH stretches. Spectral changes with cluster size demonstrate that the chain structures at small sizes (n less, similar 10) develop into two-dimensional net structures (approximately 10 < n < 21), and then into nanometer-scaled cages (n >/= 21).  相似文献   

Effects of hypoxia and glucose-removal condition on muscle contraction of the smooth muscles of porcine urinary bladder     

Yuta NAGAI  Takeharu KANEDA  Yasuyuki MIYAMOTO  Takaomi NURUKI  Hidenori KANDA  Norimoto URAKAWA  Kazumasa SHIMIZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):55-59

To elucidate the dependence of aerobic energy metabolism and utilization of glucose in contraction of urinary bladder smooth muscle, we investigated the changes in the reduced pyridine nucleotide (PNred) fluorescence, representing glycolysis activity, and determined the phosphocreatine (PCr) and ATP contents of the porcine urinary bladder during contractions induced by high K⁺ or carbachol (CCh) and with and without hypoxia (achieved by bubbling N₂ instead of O₂) or in a glucose-free condition. Hyperosmotic addition of 65 mM KCl (H-65K⁺) and 1 µM CCh induced a phasic contraction followed by a tonic contraction. A glucose-free physiological salt solution (PSS) did not change the subsequent contractile responses to H-65K⁺ and CCh. However, hypoxia significantly attenuated H-65K⁺- and CCh-induced contraction. H-65K⁺ and CCh induced a sustained increase in PNred fluorescence, representing glycolysis activity. Hypoxia enhanced H-65K⁺- and CCh-induced increases in PNred fluorescence, whereas glucose-free PSS decreased these increases, significantly. In the presence of H-65K⁺, hypoxia decreased the PCr and ATP contents; however, the glucose-free PSS did not change the PCr contents. In conclusion, we demonstrated that high K⁺- and CCh-induced contractions depend on aerobic metabolism and that an endogenous substrate may be utilized to maintain muscle contraction in a glucose-free PSS in the porcine urinary bladder.  相似文献   

Renal expression and urinary excretion of liver-type fatty acid-binding protein in cats with renal disease     

Masaaki Katayama  Keiichi Ohata  Tamako Miyazaki  Rieko Katayama  Nobuko Wakamatsu  Misa Ohno  Tetsuro Yamashita  Tsuyoshi Oikawa  Takeshi Sugaya  Masao Miyazaki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(2):761-769

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