首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   16767篇
  免费   1240篇
  国内免费   32篇
林业   1232篇
农学   822篇
基础科学   108篇
  3215篇
综合类   1059篇
农作物   673篇
水产渔业   1324篇
畜牧兽医   7722篇
园艺   253篇
植物保护   1631篇
  2023年   115篇
  2022年   118篇
  2021年   249篇
  2020年   266篇
  2019年   242篇
  2018年   650篇
  2017年   695篇
  2016年   631篇
  2015年   530篇
  2014年   618篇
  2013年   947篇
  2012年   1013篇
  2011年   963篇
  2010年   597篇
  2009年   545篇
  2008年   840篇
  2007年   745篇
  2006年   630篇
  2005年   632篇
  2004年   542篇
  2003年   531篇
  2002年   430篇
  2001年   463篇
  2000年   411篇
  1999年   373篇
  1998年   116篇
  1997年   110篇
  1996年   101篇
  1995年   88篇
  1992年   223篇
  1991年   208篇
  1990年   208篇
  1989年   217篇
  1988年   192篇
  1987年   183篇
  1986年   174篇
  1985年   153篇
  1984年   134篇
  1983年   116篇
  1981年   92篇
  1980年   89篇
  1979年   161篇
  1978年   126篇
  1977年   127篇
  1976年   103篇
  1975年   91篇
  1974年   139篇
  1973年   135篇
  1972年   119篇
  1971年   122篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
191.
Landscape Ecology - Harmful effects of habitat loss and fragmentation can be detected across multiple spatial scales, yet most studies that aim to characterize these effects take place at a single...  相似文献   
192.
Improving Drought Tolerance of Rice by Designed QTL Pyramiding   总被引:4,自引:0,他引:4  
Drought is the most important factor limiting rice yields in the rainfed areas of Asia. To overcome the problem, we developed a new strategy 'designed QTL pyramiding' to more efficiently develop drought tolerant (DT) rice cultivars. In the first phase of this strategy, we developed large numbers of introgression lines (ILs) in elite backgrounds using backcross (BC) breeding, each of which carries multiple genomic segments for improved DT from a known donor. Then, we genotyped all ILs with SSR markers to track the genomewide pattern of introgression in the DT ILs of 16 BC populations derived from crosses between 2 recipients (IR64 and Teqing) and four different donors. X^2 tests and linkage disequilibrium analyses of introgression in the DT ILs revealed significant frequency shifts at many loci across the genome and non-random associations at the multilocus level as a result of selection for DT in the ILs, which led us to the discovery of putative genetic networks underlying DT in the ILs. The networks each containing all DT loci detected in ILs from a BC population showed some interesting features.  相似文献   
193.
Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis, cis-1,4-pentadiene structure producing hydroperoxy acids with conjugated dienes. LOX activity has been found in a wide range of plants. Typical substrates for LOX in plants are linoleic acid and linilenic acid fatty acids. The function of various LOXs in plants is unknown, but their participation in all stages of plant growth and development has been suggested (Hildebrand, 1989; Siedow, 1991). Some of the physiological processes in whicn lipoxygenses have been implicated include wounding (Saravitz and Siedow, 1996), pathogen attack (Melan et al., 1993), seed germination (Kato et al., 1992), fruit ripening (Ferrie et al., 1994), plant senescence (Paliyath and Droillard, 1992). The study on the role of lipoxygenase in ripening and senescence fruit focused on tomato and strawberry. Cloning LOX gene of cucumber fruit will make us further understand the molecularaction mode of this enzyme during fxuit ripening and senescence. In this paper we isolated the partial nucleotide sequences of cucumber fiuit lipoxygenase gene and discuss the characterization of it.  相似文献   
194.
This study assesses the effect of salinity in bioavailability and toxicity of Zn by means of laboratory bioassays by observing contamination in both sediment and water, accumulation of Zn in biological tissues, and histopathological damage in the gills and guts tissues of Ruditapes philippinarum clams, which were exposed to different types of sediments from the Gulf of Cádiz (SW Spain) as well as two dilutions of toxic mud coming from an accidental mining spill. With this objective, the coefficients of distribution (K D) for Zn between overlying water and sediments were calculated, the histopathological frequencies in the tissues of the gills and guts of clams were determined, and the biota-sediment bioaccumulation factors as well as the bioaccumulation factors were quantified in the different stations. Results showed that the greatest histopathological damages appeared when the salinity values decreased. Statistical results showed that salinity was inversely correlated with histopathological damage (p?<?0.01) for the lesion index for gills. The most outstanding results were observed in the two dilutions of toxic mud (0.3% and 7.9%) at a salinity value of 10. Salinity was inversely correlated with the concentration of Zn in biological tissues (p?<?0.05) and inversely correlated with the concentration of Zn in water and sediment. Zn mobilization to the overlying water is produced when salinity values decrease.  相似文献   
195.
An inexpensive and portable surface plasmon resonance (SPR) sensor, SPReeta Evaluation Kit SPR3, has been used to develop a biosensor for the determination of fluoroquinolone antibiotics (FQs) and to demonstrate its performance analyzing FQ residues in milk samples. The SPReeta three-channel gold chips were activated with a mixed self-assembled monolayer (m-SAM) and functionalized with a FQ haptenized protein. Binding of the antibody produced a concentration-dependent increase of the SPR signal as a result of the change in the refraction index. Similarly, the presence of the FQ produced a dose-dependent decrease of the response, which allowed a good limit of detection (LOD) to be obtained (1.0 ± 0.4 μg L(-1) for enrofloxacin in buffer). The response was reproducible in all three channels, on different injections and days, and also between chips. Milk samples could be analyzed after a simple sample treatment involving fat removal by centrifugation and dilution with water. Under these conditions calibration curves were obtained showing that FQ residues can be analyzed in milk samples with an IC(50) value of 26.4 ± 7.2 μg L(-1) and a LOD of 2.0 ± 0.2 μg L(-1) (for enrofloxacin), far below the European Union regulations for this antibiotic family in this matrix. Finally, the paper also demonstrates that the biosensor is able to selectively detect the presence of FQs in milk samples, even in the presence of other antibiotics. Enrofloxacin, ciprofloxacin, and norfloxacin residues were detected in blind samples supplied by Nestle? Co.  相似文献   
196.
This study focused on the application of native strains of arbuscular mycorrhizal fungi (AMF) and Sinorhizobium in effective crop production during reclamation of coal‐mine spoil banks. Two greenhouse experiments were conducted in spoil‐bank clay with a low dose of organic amendment to determine whether the microbial inoculation improves growth and utility qualities of two cultivars of Linum usitatissimum L. (oil and fiber flax). Inoculation with two native AMF isolates (Glomus mosseae, G. intraradices, and their mixture) significantly increased growth and shoot phosphorus (P) concentration of both flax cultivars. Inoculated fiber flax plants produced fivefold more fibers than the uninoculated ones. In oil flax, mycorrhizal inoculation significantly but quantitatively to a minor degree decreased the concentration of nonsaturated fatty acids in the seed oil. A mixture of five native Sinorhizobium sp. strains supported growth and P uptake of oil flax only in the absence of AMF. However, these beneficial effects of the bacteria were significantly lower as compared to AMF. No synergic action of Sinorhizobium strains and AMF was observed, and their interactions were often even antagonistic. Inoculation with AMF significantly decreased population density of Sinorhizobium in the soil. These results suggest that a careful selection of suitable bacterial strains is necessary to provide effective AMF combinations and maximize flax‐growth support.  相似文献   
197.
Sunflower broomrape (Orobanche cumana Wallr.) is a parasitic plant that infects sunflower (Helianthus annuus L.) plants. In this work, sunflower plants were grown under greenhouse conditions in pots with the substrate infested or non-infested with broomrape seeds. At different numbers of days after sowing, plant height, internode lengths, number of leaves, head diameter, mineral composition of leaves, and potassium (K) concentration in stem were measured. The negative effects of broomrape parasitism were assessed from 57 d after sowing, when broomrape started to emerge. Parasitized plants exhibited lower shoot dry weight, height, and head diameter than control plants. The reduction in internode lengths was associated with a decrease in the gradient of K concentration from basal to apical stem. The mineral composition of leaves was also affected in parasitized plants. The concentrations of calcium (Ca), magnesium (Mg), manganese (Mn), and zinc (Zn) in leaves of parasitized plants were lower than those of the control plants, while there were few differences for K, phosphorus (P), iron (Fe), and copper (Cu). The effects of parasitism are discussed in relation to their competition for resources and to perturbations of the host physiology such as hormonal and water balance.  相似文献   
198.
Nutrient solution composition plays an important role in root uptake rate due to interactions among nutrients and internal regulation. Studies to determine the optimum nutrient solution concentration are focused on individual ions, ignoring the adaptation mechanisms triggered by plants when growing in a varying external nutrient concentration. The objective of the present study was to determine the response in growth and tissue ion concentration of lilium cv. ‘Navona’ to nutrient mixtures of varying proportions of nitrogen (N), potassium (K+), and calcium (Ca2+) in solution using mixture experiments methodology in order to determine the optimum concentration. Bulbs of lilium were transplanted in plastic crates and drip-irrigated with the treatment solutions, which consisted of a mixture of N, K+, and Ca2+ whose total concentration was 340 mg L?1 and minimum concentrations of each ion was 34 mg L?1. Chlorophyll concentration (SPAD), shoot fresh weight (FW), leaf FW, and leaf area were measured 60 days after transplanting and ion analysis was performed on shoot tissues from selected treatments. Lilium exhibited a moderate demand for N and K+ (136–170 mg L?1 N and 116–136 mg L?1 K+) and a very low demand for Ca2+ (34–88 mg · L?1). This low demand may be due to the remobilization of the nutrients stored in the bulbs. Integrating the predictions of the models estimated to produce >90% of maximum growth, the optimum nutrient solution should contain Ca2+ at a concentration between 34 and 126 mg · L?1, K+ between 119 and 211 mg · L?1, and N between 92 mg · L?1 and 211 mg · L?1. Increasing external N concentration affected internal N concentration but not internal K+ or Ca2+ concentrations, despite that the increase in external N was associated with a decrease in external K+ and Ca2+. Similar trends were observed for external K+ and Ca2+ concentration. In conclusion, lilium was able to maintain a relatively constant K+ and Ca2+ concentration regardless of the lower concentration in the nutrient solution when N was increased (similar response was observed for K+ and Ca2+) and it has a low Ca2+ demand and moderate N and K+ supply.  相似文献   
199.
200.
Tomato (Solanum lycopersicum Mill) is an important crop in terms of its economic and nutritional value. Many factors, including cultivar, climate, geography, geochemistry, and agricultural practice, can affect its nutrient concentrations. An HJ‐biplot study was performed to examine the effects of cultivar (Dorothy, Boludo, Dominique, Thomas, and Dunkan), agricultural practices, climatic factors, and their interactions. Significant differences were analyzed using a one‐way ANOVA. All samples were collected and assayed at the same degree of ripeness. In the conventional and organic tomato samples, those harvested from December to April had the highest concentrations of fructose, glucose, citric acid, malic acid, ascorbic acid, protein, Na, and Mg, while those harvested in October had the highest concentrations of lycopene and hydroxycinnamic acid. There were high concentrations of Ca, P, Zn, and Cu in the no‐soil tomatoes. Conventional and organic cultivation practices showed similar results with respect to the collection period, both presenting high organic compound concentrations, while high mineral concentrations seemed to correspond to the no‐soil practice. No clear pattern was observed among the different cultivars, perhaps due to all the samples having been collected at the same degree of ripeness.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号