Anaplasmosis in Uganda. I. Use of dried blood on filter papers and serum samples for serodiagnosis of anaplasmosis—A comparative study     

G. S. Z. Ssenyonga  I. Kakoma  Sonia Montenegro-James  R. Hansen 《Tropical animal health and production》1992,24(1):2-8

Summary The suitability of blood collected on filter papers in comparison with corresponding conventional serum samples in the diagnosis of bovine anaplasmosis was studied using the complement fixation test, DOT-ELISA, Western immunoblot and rapid card agglutination test. Dried blood on Whatman filter paper no. 1 was eluted in PBS 0·05% Tween 20 giving an initial dilution of 1∶10. The reactivity of the eluted samples in both DOT-ELISA and Western immunoblotting were similar to those obtained with the corresponding straight serum sample dilutions. Filter paper samples gave lower reactivity in the remaining tests when compared with corresponding serum samples. There was no significant difference in the reactivity between the eluates from filter papers stored at temperatures ranging between 15·5 and 24°C and those kept refrigerated. Storage at 15·5 to 24°C did not significantly affect reactivity for up to six months. Eluates from filter papers stored for six months at 15·5 to 24°C continued to give similar reactivity as those from freshly prepared filter papers in both DOT-ELISA and Western blot, and in the rapid card agglutination test. It is concluded that collecting blood on filter papers is a suitable technique for large scale seroepidemiological studies on anaplasmosis and offers many advantages in developing countries where transport and cold chain facilities are a major constraint.

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Resumen Se estudió la efectividad de muestras de sangre colectadas en papel filtro, en comparación con las correspondientes muestras convencionales du suero, en el diagnóstico de anaplasmosis bovina, utilizando fijación de complemento, DOT-ELISA, Western, immunoblot y la prueba rápida de la tarjeta. La sangre seca en papel Whatman N^o 1 fue removida con PBS 0·05% entre 20, dando una dilución inicial de 1∶10. La reactividad de las muestras removidas de papel filtro, en la prueba de DOT-ELISA y Western immunoblotting, fueron similares a la obtenida con la correspondiente muestra de suero. Las muestras de papel filtro reaccionaron menos en las otras pruebas, cuando se compararon con las correspondients muestras du suero. No hubo diferencia significativa en la reactividad entre los lavados del papel filtro guardados a temperaturas entre 15·5 y 24°C y aquellos guardados en refrigeración. El almacenaje entre 15·5 y 24°C, no afectó la reactividad hastas seis meses. Los lavados de papel filtro guardados por seis meses entre 15·5 y 24°C, dieron la misma reactividad como los lavados frescos, en la prueba DOT-ELISA y Western blot, lo mismo que en la prueba de aglutinación rápida de tarjeta. Se concluye, qu la colección de sangre en papel filtro, es una buena técnica para estudios epidemiológicos de cierta magnitud, sobre anaplasmosis, ofreciendo ventajas considerables en paises en desarrollo en donde las cadenas de frío son deficientes.

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Résumé La fiabilité du sang récolté sur papier filtre comparée à celle des prélèvements conventionnels de sérum pour le diagnostic de l'anaplasmose a été étudiée à l'aide des tests suivants: fixation du complément, ELISA, immunoblot de Western, test rapide d'agglutination sur carte. Du sang séché sur papier filtre Whatman N^o 1 a fait l'object d'une élution dans une solution de PBS à 0,05 p. 100 (Tween 20) pour donner une dilution de base au dilution de base au 1∶10. Le réactivité des échantillons, autant avec le test ELISA que l'immunoblot Western, a été identique à celle obtenue par dilution directe de sérums homologue. Les échantillons sur papier filtre ont donné une réactivité plus faible pour les autres tests, comparée à celle des échantillons de sérum semblables. Aucune différence significative n'a été décelée quant à leur réactivité les éluats provenant de papiers filtres stockés à des températures comprises entre 15,5 et 24°C at ceux conservés au réfrigérateur. Le stockage entre 15,5 et 24°C n'a pas non plus affecté la réactivité de fa?on significative; les éluats conservés à partir des papiers filtres, à cette même température durant 6 mois, ont montré des réactions identiques que ceux provenant de papiers filtres fra?chement préparés, à la fois avec le test ELISA, celui de Western Blot et le test d'agglutination rapide sur carte. On peut conclure que la collecte du sang sur papier filtre est une technique adaptée à l'étude épidémiologique de l'anaplasmose à grande échelle. Elle offre de nombreux avantages dans les pays en développement où offre de nombreux avantages dans les pays en développement où les moyens de transports et la cha?ne du froid constituent des contraintes majeures.

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A device for determining the sorptivity of soil aggregates   总被引：2，自引：0，他引：2  

P.B. LEEDS-HARRISON  E.G. YOUNGS  B. UDDIN 《European Journal of Soil Science》1994,45(3):269-272

A device that infiltrated water over a small circular surface area having a radius ranging between 1.45 mm and 2.5 mm was used to measure the sorptivity of initially air-dry aggregates of size greater than 20 mm. The small infiltration area caused the water uptake to be dominated by capillarity that allowed the use of a simple formula to obtain the sorptivity from the steady-state flow rate that occurred very early after the start of imbibition. The results of measurements of sorptivity made on a fine sand agreed with those obtained from measurements on one-dimensional water infiltration into columns of the sand. Sorptivity measurements on stabilized aggregates of a clay soil and on air-dried field aggregates of a clay soil were easily made. The simplicity and rapidity of the method allowed measurements to be conveniently replicated.  相似文献   

菲律宾以稻为基础的种植制度生态学评价 Ⅰ.各类种植方式的养分平衡     

赵强基 Garr.  DD 《作物学报》1993,19(5):402-411

在菲律宾的雨养旱地、雨养低地和灌溉田种植制度研究基点上,分别对各种农民和拟推广的实验种植方式,进行了生物量及其组成、作物分器官的 C、N、P、K 含量与分布比率、作物残茬燃烧后 C、N、P、K 损失测定。从添加与取走两个方面,分三种添加与取走情况,计算了土壤N、P、K 的部分养分平衡与有机质平衡。结果显示:在当地施肥水平下,即使归还全部残茬,很难维持 N、P、K 的养分平衡,但能较好满足土壤有机质平衡。为维持土壤有机质的年平衡,雨养低地和雨养旱地需分别归还1400和1900公斤生物物质/公顷.年。并对当地维持养分平衡的途径作了分析与探讨。  相似文献   

The diagnosis of nephrogenic diabetes insipidus in the dog]     

E G Grünbaum  A Moritz 《Tier?rztliche Praxis》1991,19(5):539-544

Nephrogenic diabetes insipidus is characterized by polyuria, hyposthenuria and compensatory polydipsia. With conventional clinical and laboratory examinations the diagnosis can be established and the differential diagnostic diseases excluded. In order to verify the diagnosis, differentiate the condition from central and nephrogenic diabetes insipidus and rule out psychogenic polydipsia, additional laboratory studies are required: of these, the concentration test, modified Carter-Robbins test and AVP stimulation test are described.  相似文献   

调整财政政策大力支持林业的跨越式发展     

赵鸣骥 《绿色中国(A版)》2001,(3):18-22

根据党的十五届五中全会和中央经济工作会议精神 ,根据“十五”期间财政改革的总体部署和要求以及实现林业新世纪跨越式发展的任务 ,2 0 0 1年以及今后一个时期财政部门的林业财务工作应当重点抓好以下几件事 :　　一、继续深化改革 ,调整林业财务管理工作的思路　　 (一 )按照财政宏观管理职能的要求 ,集中精力抓大事。财政作为宏观管理部门的职能和地位 ,决定了我们在工作中必须牢固树立全局的观念 ,善于从全局的高度分析和处理问题 ,因此 ,必须集中精力抓好大事。所谓大事 ,就是参与研究、制定林业的重大方针政策 ,包括 :林业的发展规划…  相似文献   

Intradermal testing of horses with chronic obstructive pulmonary disease and recurrent urticaria.     

A G Evans  M R Paradis  M O'Callaghan 《American journal of veterinary research》1992,53(2):203-208

Six horses with chronic obstructive pulmonary disease (COPD) and 8 horses with recurrent urticaria were skin tested with 67 extracts from 58 allergens, including pollens, epidermals, cultivated farm plants, dusts, molds, and insects. Reactions were evaluated 3 times over a 24-hour period immediately after the injections. Results were compared with those obtained from 11 clinically normal horses. All horses had positive skin test reactions. Significant differences was evident between horses with COPD and clinically normal horses for only 3.0% of the possible extract reactions, and between horses with urticaria and clinically normal horses for only 4.5% of the possible extract reactions. Horses with COPD or urticaria had greater total percentage of allergen extract reactions than did clinically normal horses. Positive reactions were observed at all 3 evaluation periods, and late-onset reactions were not always preceded by positive reaction at earlier periods. All horses with COPD or urticaria had at least 1 skin test reaction that exceeded the mean +/- 2 SD, as calculated for each of the 67 extracts for the group of clinically normal horses.  相似文献   

百脉根种子硬实处理与耐藏性的探讨   总被引：8，自引：2，他引：6  

宋淑明  聂朝相 《草业科学》1994,11(5):52-54

地百脉根种子硬实用不同方法处理和对不同贮藏时间种子耐藏性的测定结果表明：贮藏１年种子含硬实率５９．３％。经擦破种皮或硫酸浸种处理，硬实率明显下降，发芽势，发芽率显著提高；种子随贮藏期的增长，硬实率明显下降，其发芽势，发芽率，活力指数以及直播出苗率，幼细苗存活率等亦随贮藏时间的延长而下降；种子电导值则随贮藏时间的延长而增大。  相似文献   

Release of tumor necrosis factor-alpha from bovine alveolar macrophages stimulated with bovine respiratory viruses and bacterial endotoxins     

S.E. Bienhoff  G.K. Allen  J.N. Berg 《Veterinary immunology and immunopathology》1992,30(4):341-357

The release of tumor necrosis factor-alpha (TNF-) from cultured bovine alveolar macrophages (BAM) was evaluated following stimulation of BAM with bovine herpesvirus-1 (BHV-1), parainfluenza-3 (PI-3) virus, bovine respiratory syncytial virus (BRSV), Escherichia coli 0111:B4 endotoxin, Pasteurella haemolytica type 1 endotoxin, Pasteurella multocida endotoxin, and virus/endotoxin combinations. A cytotoxic assay system using Georgia bovine kidney cells as targets was used to measure TNF- activity. The cytotoxic activity was neutralized by an anti-human TNF- monoclonal antibody.

Stimulation of BAM with 1 median tissue culture infectious dose (TCID₅₀) of live or ultraviolet (UV)-inactivated PI-3 virus/cell resulted in release of TNF- in significantly (P<0.05) higher amounts than sham-induced BAM. The quantities of TNF- released after live or UV-inactivated BHV-1 or BRSV induction were not significantly higher than sham-induced BAM. E. coli 0111:B4, P. haemolytica type 1 and P. multocida endotoxins stimulated TNF- release in a dose-dependent manner. Sequential exposure of BAM to 1 TCID₅₀ per cell of either live BHV-1, PI-3 virus or BRSV and then 5 μg ml⁻¹ of either E. coli 0111:B4, P. haemolytica type 1 or P. multocida endotoxin caused a significant (P<0.05) reduction in detectable TNF- in seven of nine virus/endotoxin combinations tested, when compared with 5 μg ml⁻¹ of endotoxin alone. Parainfluenza-3 virus/endotoxin combinations stimulated higher TNF- release when compared with other virus/endotoxin combinations. Five out of six test animals had serum-neutralizing antibodies to PI-3 virus, one out of six had serum-neutralizing antibodies to BHV-1, and two out of six had serum-neutralizing antibodies to BRSV, suggesting a possible relationship between serum neutralizing antibodies and TNF- release from in vitro cultivated BAM.  相似文献   

大豆室内耐冷筛选及其在田间的应用研究   总被引：4，自引：1，他引：3  

李育军  常汝镇 《大豆科学》1992,11(1):49-57

本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。  相似文献   

Potential of a recombinant antigen as a prophylactic vaccine for day-old broiler chickens against Eimeria acervulina and Eimeria tenella infections.   总被引：5，自引：0，他引：5  

B S Bhogal  G A Miller  A C Anderson  E J Jessee  S Strausberg  R McCandliss  J Nagle  R L Strausberg 《Veterinary immunology and immunopathology》1992,31(3-4):323-335

A genetically engineered Eimeria tenella antigen (GX3262), produced as a fusion protein with beta-galactosidase and identified with a monoclonal antibody, induced partial but significant protection in young broiler chickens against experimental E. tenella and Eimeria acervulina infections. The antigen appears to share a T-helper cell epitope with the parasite as evidenced by (a) booster inoculation with either the recombinant antigen or with a small number of live oocysts enhanced the protective immunity in GX3262 primed chickens, and (b) ability of the antigen to induce in vitro stimulation of T-cells from chickens immunized with antigen or parasite. These observations suggest the feasibility of a single vaccination of 1 or 2-day-old broilers with GX3262 to induce an acceptable degree of protective immunity. The implications of the observations reported here are far reaching in terms of a practical coccidiosis vaccine for poultry, and show for the first time that 1-day-old broiler chickens can be efficiently vaccinated with a recombinant antigen against one or more species of Eimeria.  相似文献