气候变化对内蒙古农牧业生态环境的影响   总被引：2，自引：1，他引：2  

陈素华  宫春宁  苏日那 《干旱区资源与环境》2005,19(4):155-158

分析了数十年来气候变暖对内蒙古农牧业生态环境的影响。发现气候变暖使内蒙古地区无霜期延长,积雪、冰雹、雷暴、大风、沙尘暴日数减少,降水量也出现由少转多的变化。事实表明,气候增暖使内蒙古总的生态环境出现向好转化,但是增暖所带来的负面影响是病虫害的增加,加上人为的作用,严重地破坏了内蒙古的生态平衡。  相似文献   

The comet assay of peripheral blood lymphocytes in patients with chronic hepatitis B and hepatocellular carcinoma     

HE Wei  TANG Yong-huang  LIANG Xu-jing  HUANG Yao-xiong  ZHANG Li-fa  GONG Xiao-bing  CAI Dong-qing 《园艺学报》2005,21(4):820-821

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Effects of NK cells on the etiology of severe pre-eclampsia     

ZHANG Zhan  JIA Li-ting  HOU Lei  WANG Xue-mei  CHANG Cai-hong  YANG Ru-jing  ZHANG Xi  LIU Sheng-hong  ZHENG Fang  GONG Fei-li 《园艺学报》2005,21(12):2457-2461

AIM: To detect the effects of NK cells on the etiology of severe pre-eclampsia. METHODS: The peripheral blood and umbilical blood from severe pre-eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by ［51Cr］ releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA-G in placenta of them. RESULTS: (1) Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood, umbilical blood and decidua of severe pre-eclamptic patients were significantly higher (all P<0.05). (2) Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre-eclamptic patients were significantly higher. (3) The expression of HLA-G protein in placenta of severe pre-eclampsia group was lower than that in normal group (P<0.05). CONCLUSION: The quantity of NK cells in severe pre-eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre-eclampsia.  相似文献   

牛分枝杆菌抗原MPB70、MPB83和ESAT-6的融合表达及重组蛋白的初步应用   总被引：14，自引：1，他引：14  

郭设平  刘思国  张秀华  王春来  宫强  郭洋  邵美丽 《畜牧兽医学报》2006,37(7):676-680

应用PCR方法从牛分枝杆菌Vallee株基因组中扩增获得mpb70、mpb83和esat-6三个目的基因片段。采用重叠延伸剪接技术(splicing by overlap extension,SOE)获得融合基因mpb70-mpb83后,将mpb70-mpb83和esat-6串连于同一表达载体pET32a( )中得到重组质粒pET70-83-E6。转化BL21(DE3)大肠杆菌感受态后,经IPTG诱导以可溶的形式表达融合蛋白。用Ni2 亲合层析的方法纯化该融合蛋白。Western blot分析显示:该融合蛋白能与抗牛分枝杆菌阳性血清发生特异性反应,而与牛副结核病阳性血清不反应。用该纯化蛋白初步建立了间接ELISA方法,并检测了117份临床血清样本(其中67份为PPD阳性牛血清),阳性率为39.32%(46/117)份,与PPD皮试诊断的符合率为82.05%(96/117)。  相似文献   

猪传染性胸膜肺炎重组亚单位疫苗诱导小鼠免疫保护效果的观察     

邵美丽  刘思国  王春来  王勇  宫强  尹训南  郭设平  刘建东  佟恒敏 《畜牧兽医学报》2006,37(10):1021-1026

重组表达猪传染性胸膜肺炎放线杆菌6种主要毒力因子基因:apxⅠ、apxⅡ、apxⅢ、apxⅣ、apfa和omp,以重组蛋白rApxⅠ、rApxⅡ、rApxⅢ和rOMP组合免疫小鼠作为试验I组,重组蛋白rApxⅠ、rApxⅡ、rApxⅢr、OMPr、ApxⅣ和rApfa组合免疫小鼠作为试验Ⅱ组,PBS为对照组,分3次免疫小鼠,采用背部皮下多点注射,每次间隔2周,免疫剂量为0.2 mL/只,3免后1周分别以APP1型菌Shope 4074株(5×109cfu)和APP2型菌S1536株(5×1010cfu)进行攻毒试验。通过小鼠保护率与抗体效价的相关性研究、肺部病理变化及肺脏细菌的分布情况等指标进行综合评价。结果显示,试验Ⅰ组4种重组蛋白特异性抗体水平显著高于其他两组(P<0.05),对APP1型菌攻毒的保护率(9/10)明显高于试验Ⅱ组(5/10)和对照组(0/8),小鼠的免疫保护率与抗体效价之间存在显著正相关;且该组对APP2型菌攻毒的保护作用(无肺脏损伤)也明显优于其它两组(典型肺部损伤)。间接免疫荧光试验表明试验Ⅰ组对肺脏细菌的清除效果也明显优于其他两组。本试验揭示试验Ⅰ组对不同血清型APP攻击能够提供很好的交叉保护作用,从而为猪传染性胸膜肺炎新型疫苗的研制提供参考。  相似文献   

鲁西黄牛体细胞克隆保种技术的研究     

朱化彬  程金华  孙秀柱  王栋  戴蕴平  龚国春  万荣  郝海生 《畜牧兽医学报》2006,37(12):1287-1292

以体外培养的鲁西黄牛耳皮肤成纤维细胞作核供体，研究利用体细胞克隆技术保存我国地方黄牛优良品种的技术方法。通过组织块培养法建立的鲁西黄牛（1♂，4♀）成纤维细胞系，作为核移植供体细胞，利用屠宰场母牛卵巢卵母细胞经体外培养成熟后作为核受体进行核移植，试验结果表明：重构胚的融合率为62．5％（242／387），分裂率为63．6％（154／242），体外培养第7天囊胚发育率为42．9％（66／154）。体外培养第7天的囊胚的内细胞团细胞（ICM）与滋养层细胞数平均为37和47，ICM占44．2％。体外发育到7d的囊胚新鲜胚胎的移植妊娠率（新鲜胚胎）移植受体10头，60d妊娠率为20％（2／10）。  相似文献   

霍乱弧菌封闭带毒素功能片段△G的原核表达及其免疫增强活性     

焦玉萍马静云  谢青梅曹永长周庆丰  胡轶鹏王静 毕英佐 《中国兽医科技》2007,37(2):135-139

用PCR扩增了MBP—ZOT质粒上的ZOT基因，该基因编码264～399位氨基酸残基C末端的15ku △G片段，并与经改造后的质粒P^BCX、连接。将重组后的质粒PMLAG在大肠杆菌BL21中表达，将表达的目的蛋白纯化，再将p^BCX-△G片段与传染性腔上囊炎病毒VP2包涵体蛋白通过滴鼻免疫途径对14日龄SPF鸡进行联合免疫试验，分别于免疫后第10、20d采集血清样品，进行ELISA试验，检测免疫后IBD抗体效价。动物免疫试验发现：融合蛋白P^BCX △G起到了明显免疫增强效果，与VP2重组蛋白联合免疫2次后，血清IBD的VP2 IgG抗体效价是单独用VP2免疫组的3倍，明显高于空白免疫组。  相似文献   

激素免疫法对家畜生殖内分泌的调节及其应用     

马惠海  巩俊明  李艳  赵玉民  代春玲  张利  杨世娟 《吉林畜牧兽医》2007,28(5):10-13

激素免疫中和技术基于激素的内分泌机制,利用激素免疫动物,改变动物体内激素正常代谢水平,以达到提高动物生产性能的目的。目前该项技术已在动物繁育上显示出很大的潜力,它在提高动物繁殖和应用于免疫去势等方面显示出优势。作者主要综述了激素免疫的作用机理在畜牧生产的应用以及影响激素免疫效果的因素等研究内容。  相似文献   

Effect of Supplementing Alfalfa Powder to Diet on Growth Performance and Meat Quality of Goose     

YAN Chunchi  DONG Biao  ZHAO Minmeng  LIU Long  GENG Tuoyu  GONG Daoqing  WANG Jian 《中国畜牧兽医》2007,47(11):3502-3509

The experiment was aimed to investigate the effects of dietary alfalfa powder on the growth performance,body size,slaughter performance,organ index and meat quality of Siji geese.A total of 256 15-day-old geese (half male and half female) with similar body weight were randomly divided into four groups with 4 replicates of 16 geese each.The geese in control group were fed with corn-soybean meal-based diet (control diet),and geese in the experimental groups were fed the basal diet replaced with 10%,20% and 30% alfalfa powder,respectively.The results showed that,compared with the control group,dietary alfalfa powder supplementation had no significant effect on the average daily intake,average daily gain,feed conversion ratio,body oblique length,keel length,shin circumference,semi diving length,chest width and shin length (P>0.05),and also had no effect on the carcass ratio,half-eviscerated carcass ratio and pectoral muscle ratio (P>0.05).However,dietary 20% and 30% alfalfa powder supplementation significantly increased the chest depth (P<0.05),dietary 30% alfalfa powder significantly decreased the full-eviscerated carcass ratio (P<0.05),and dietary 10% alfalfa powder significantly increased the leg muscle ratio (P<0.05) of geese.In addition,dietary alfalfa powder significantly decreased the abdominal fat ratio (P<0.05),raised the growth index of liver,jejunum,ileum,and cecum (P<0.05),and increased the water loss rate of pectoral muscle and leg muscle (P<0.05) compared with the control group.Dietary 10% and 30% alfalfa powder significantly increased duodenal growth index (P<0.05) of geese.In conclusion,dietary alfalfa powder supplementation might improve the slaughter performance (increase chest depth and leg muscle ratio,reduce abdominal fat rate) and organ growth index (increase the growth index of liver,duodenum,jejunum,ileum and cecum).However,dietary alfalfa powder increased water loss rates in pectoral muscle and leg muscle,and 30% alfalfa powder reduced the full-eviscerated carcass ratio of geese.The appropriate supplementation level of alfalfa powder was recommended at 10% in the present study.  相似文献   

猪囊尾蚴特异性单克隆抗体的制备及其识别抗原成分的鉴定     

石团员  宁长申  张改平  王选年  张龙现  李青梅  职爱民  菅复春  蔡书军  乔宏兴 《中国兽医学报》2007,27(6):853-857,962

通过SDS-PAGE方法对猪囊尾蚴囊液抗原成分进行了分离鉴定,并分别分离纯化了其中的16 000和10 000特异性蛋白质成分;将16 000和10 000纯化蛋白质成分分别做成佛氏佐剂苗,分组免疫BALB/c小鼠,超免后,无菌取脾脏制备脾细胞,分别与NS0骨髓瘤细胞进行融合,经阳性筛选和特异性鉴定获得2株分泌猪囊尾蚴特异性单抗的杂交瘤细胞,命名为TSCF-1611H12B8和TSCF-1012G5B5。免疫学鉴定结果表明,单抗TSCF-1611H12B8和TSCF-1012G5B5与猪细颈囊尾蚴、旋毛虫、住肉孢子虫和蛔虫抗原间不存在交叉反应;单抗TSCF-1611H12B8识别囊液中16 000和10 000蛋白质抗原条带,而单抗TSCF-1012G5B5识别囊液中的10 000蛋白质条带。  相似文献