Effects of component of some Chinese herbs on proliferation of human umbilical vein endothelial cells in vitro     

HE Xiu-juan  LI Ping  QIU Quan-ying  SHENG Xun  WANG Fang 《园艺学报》2004,20(5):832-835

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair.  相似文献   

Effects of puerarin on inducible nitric oxide synthase in lens during diabetic cataract in rats     

HAO Li-na  LING Yi-ling  GU Zhen-yong  HUANG Xin-li  HE Shou-zhi 《园艺学报》2004,20(4):620-626

  相似文献   

The molecular mechanism of inhibition of murine Lewis lung carcinoma metastasis by weimaining in vivo     

LOU Jin-li  LIN Hong-sheng  QIU Quan-ying  PEI Ying-xia  QI Xin  HE Xiu-juan 《园艺学报》2004,20(4):627-630

AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg·kg^-1·d^-1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue.  相似文献   

Radioligand binding assay of insulin receptor in rabbit kidney during ischemic and reperfusion     

HE Tao  XIE Hua-fu  DUAN Cheng-gang  LI Rong-hui  FENG Zhi-qiang 《园艺学报》2004,20(5):866-869

AIM: To observe the change of insulin receptor in rabbit kidney with acute ischemic-reperfusion injury. METHODS: 15 Japanese white rabbits were allocated randomly into control group, ischemic-reperfusion group(IR group). IR group received clamping for 1 h followed by 2 h or 48 h of reperfusion. At 2 h or 48 h after reperfusion, glucose and insulin in serum were determined. Insulin receptor in renal tissue was analyzed by radioligand binging assay(BAD). RESULTS: The level of serum glucose increased after 2 h reperfusion in 2 groups, but in IR group the value increased much more higher than those in control groups(P＜0.05). Plasma insulin of IR group was significantly higher than that in control after 2 h reperfusion(P＜0.05). Scatchard analysis of data resulted in curvilinear profiles, indicating that there are two classes of receptors with different affinity or the presence of a single class of receptors with a negative cooperative hormone-receptor interaction. Data analyzed for a two-site model showed that the values of Bmax₁(high affinity site), Bmax₂(low affinity site) and Kd₁, Kd₂ were significantly lower than that of control (P＜0.05) after 2 h perfusion. 48 h after IR there was no difference of Bmax₁, Bmax₂, and Kd₁ between 2 groups,but Kd₂ of IR group was higher than that of control (P＜0.05). CONCLUSIONS: The results indicate that the effect of intrinsic insulin decreases in the progress of the renal ischemic-reperfusion. The resulting high serum glucose may aggravate renal injury in the progress of ischemic-reperfusion.  相似文献   

鹅细小病毒VP2基因在大肠杆菌中的表达及纯化   总被引：2，自引：0，他引：2  

贺云霞  王君伟  王立群  Ulrich Neumann 《中国兽医学报》2004,24(2):126-128

将鹅细小病毒 (GPV) VP2基因插入原核表达载体 p PROEX- HTb,获得重组表达质粒 p PROEX- HTb- VP2。将其转化大肠杆菌 DH5α,用 IPTG诱导表达 ,表达菌体蛋白中可产生与预期大小相符的约 72 0 0 0的蛋白。以光密度扫描对该表达产物进行定量分析 ,表达产物约占菌体总蛋白的 14 %。经 His- tag金属螯合层析纯化 ,获得纯度较高的 6 His- VP2融合蛋白。 Western- blot结果表明 ,所得蛋白与鹅抗 GPV高免血清有较好的免疫反应性 ,说明在 VP2的 N端融合 6个组氨酸不影响其与特异抗体结合的活性。  相似文献   

苜蓿秋眠性研究进展   总被引：6，自引：9，他引：6  

何云  刘圈炜  王成章  王彦华  潘俊良  陈继红 《草业科学》2005,22(11):25-30

苜蓿Medicago sativa的秋眠性实质上是在秋季日照变短和温度降低时植株所表现出的一种适应性生长特性,这种生长特性与植物的抗寒性和生产性能相关.早在20世纪20年代,美国就发现了这一现象,但直至20世纪70年代末期,随着秋眠性测定方法的发明和分类标准的制订,才使它在苜蓿生产中发挥着越来越重要的作用,在许多国家秋眠性已成为栽培选择品种时的首要指标.概述了秋眠性的概念及发展史、测定方法、有关生理生化研究进展及其在国内外的应用.  相似文献   

乳蛋白综合调控技术研究进展     

何英俊 《畜牧与兽医》2005,37(2):49-51

乳蛋白是牛奶中最重要的成分, 是评定鲜牛奶品质的主要指标。本文从遗传、日粮营养及管理三个方面综述了国内外乳蛋白调控技术的研究进展, 为提高乳蛋白产量和含量提供依据。  相似文献   

日粮中添加杜仲提取物对肉仔鸡生产性能的影响   总被引：2，自引：0，他引：2  

吕武兴  贺建华 《饲料研究》2005,(7):3-5

选用825只艾维茵肉仔鸡,随机分为5组,每组5个重复,每个重复33只鸡。对照组饲喂基础日粮,试验组在基础日粮中分别添加1500,2000,2500g/t的杜仲提取物或50mg/kg的效美素,比较杜仲提取物对肉仔鸡生产性能及胴体品质的影响。试验分2个阶段(0～21d和21～42d),试验鸡自由采食和饮水。研究结果表明,1)日粮中添加杜仲提取物能降低采食量,提高肉仔鸡成活率,其中以添加1500g/t杜仲提取物效果较好。2)杜仲提取物能促进肌肉组织蛋白质的合成,降低体内脂肪的沉积,在一定程度上改善肉仔鸡胴体品质。  相似文献   

云岭黑山羊受体在夏季同期发情处理效果分析     

和占星  王芸  和协超  张继才  陈永昌  吴晓东  金显栋  朱芳贤  叶瑞卿  洪琼花  邵庆勇  王喆  王继  苏雷 《中国草食动物》2005,25(1):20-21

对 2 2 4只云岭黑山羊及其杂交羊在夏季用CIDR孕酮栓 PMSG法进行同期发情处理 ,结果表明 :①总体发情率为64 73 % ,移植率为 47 3 2 % ,其中发情羊在撤栓后 48小时内 10 0 %发情 ,同期化程度高 ;②楚雄羊 (n =44只 )、师宗羊 (n =96只 )、文山羊 (n =71只 )和杂交羊 (n =9只 ) ,发情率分别为 :5 2 2 7%、65 63 %、66 2 0 %和 10 0 0 0 % ,以杂交羊的同期发情率和移植率高于楚雄山羊 (P <0 0 1)、师宗山羊和文山山羊 (P <0 0 5 ) ,但三个地方的云岭黑山羊的发情率和移植率均无显著差异 (P >0 0 5 ) ;③ 1～ 2 5岁受体羊的发情率比 3～ 5岁羊高 17 74个百分点 (P <0 0 5 ) ,移植率高 11 0 6个百分点(P >0 0 5 ) ;④ 2 0～ 2 9kg羊的发情率分别比 3 0～ 3 9kg和 40～ 5 1kg的羊群高 19 2 3个百分点 (P <0 0 5 )和 2 6 0 1个百分点(P <0 0 1) ;移植率分别高 13 0 7和 14 83个百分点 (P >0 0 5 ) ;⑤青年羊的掉栓率显著低于经产羊 (P <0 0 5 ) ,而掉栓羊群和持栓羊群的发情率和移植率分别比较 ,差异不显著 (P >0 0 5 ) ;⑥处理羊在第 16天撤栓和第 17天撤栓的同期发情率差异不显著 (P >0 0 5 )。  相似文献   

纤维素酶渣替代黄豆饲喂杂交奶牛试验   总被引：1，自引：0，他引：1  

王尚荣  王放银  何华西 《中国草食动物》2005,25(2):46-47

采用纤维素酶渣替代黄豆对西黄杂奶牛(西门塔尔×本地黄牛)作饲喂对比试验,并对其产奶量及奶品质进行观察。结果表明:试验组比对照组牛日产奶量平均提高1 88kg,其乳蛋白和乳糖含量也有不同程度的提高。  相似文献