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31.
The performance (survival and growth) of Douglas-fir (Pseudotsuga menziesii Mirb. Franco.) seedlings planted in minimally disturbed, scalped, and bedded soils, both with and without herbicidal control of weeds, were compared. Douglas-fir growing for 3 years in bedded soils treated with herbicide were heavier, taller, and had deeper root systems than trees growing in other preparations. Scalping did not improve seedling performance when compared to minimally disturbed soils. Soils rich in organic matter benefited tree growth. Competing vegetation in raised beds was detrimental to seedling performance.  相似文献   
32.
The karyotypes of both Oreochromis mortimeri (Trewavas) and Sarotherodon melanotheron (Rüppell) are shown to consist of 22 pairs of chromosomes (2n = 44). In O. mortimeri, the karyotype is composed of 9 sm/st and 13 t chromosomes. In the case of S. melanotheron, the karyotype is composed of 15 sm/st, 2 m and 5 t chromosomes. The karyotypes of these species are therefore similar to those of the majority of other tilapia species, with that of S. melanotheron being considerably different from that previously reported for this species.  相似文献   
33.

Background

Serum N‐terminal pro‐C‐natriuretic peptide (NT‐proCNP) concentration at hospital admission has sufficient sensitivity and specificity to differentiate naturally occurring sepsis from nonseptic systemic inflammatory response syndrome (SIRS). However, little is known about serum NT‐proCNP concentrations in dogs during the course of sepsis.

Objective

To determine serum NT‐proCNP and cytokine kinetics in dogs with endotoxemia, a model of canine sepsis.

Samples

Eighty canine serum samples.

Methods

Eight healthy adult Beagles were randomized to receive Escherichia coli lipopolysaccharide (LPS, 5 μg/kg) or placebo (0.9% NaCl) as a single IV dose in a randomized crossover study. Serum collected at 0, 1, 2, 4, and 24 hours was stored at −80°C for batch analysis. Serum NT‐proCNP was measured by ELISA and 13 cytokines and chemokines by multiplex magnetic bead‐based assay.

Results

Serum NT‐proCNP concentrations did not differ significantly between LPS‐ and placebo‐treated dogs at any time. When comparing serum cytokine concentrations, LPS‐treated dogs had higher interleukin‐6 (IL‐6), IL‐10, TNF‐α and KC‐like at 1, 2, and 4 hours; higher CCL2 at 1, 2, 4, and 24 hours; and higher IL‐8 and CXCL10 at 4 hours compared to placebo‐treated dogs. There were no differences in serum GMCSF, IFN‐γ, IL‐2, IL‐7, IL‐15 or IL‐18 between LPS‐ and placebo‐treated dogs.

Conclusions and Clinical Importance

Serum NT‐proCNP concentration does not change significantly in response to LPS administration in healthy dogs. Certain serum cytokine and chemokine concentrations are significantly increased within 1–4 hours after LPS administration and warrant further investigation as tools for the detection and management of sepsis in dogs.  相似文献   
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Abstract – Fisheries data were collected for six species of 0-group cyprinid fishes from eight sites in the lower reaches of the River Trent, England, between May and October 1999 inclusive, using a micromesh seine net. Recruitment and growth patterns were observed via monthly length–frequency histograms and estimations of mean length. In the cases of roach and dace, growth was approximately linear through the summer, before declining in September and October. In the cases of chub and gudgeon, and to a lesser extent bream and bleak, however, there were suggestions of multiple recruitment events. It is argued that some of the fish species examined in the present study adopt fractional or protracted spawning strategies in the lower River Trent.  相似文献   
36.
Batches of eggs (1 batch/female) from 17 rainbow trout in their first spawning season were stripped and reared separately, and the percentage which hatched and the percentage which reached the stage of first feeding were determined. The fertilised egg batches were analysed for egg wet weight, egg dry weight, chorion weight and levels in the eggs of free, bound and total lipid, precipitable protein, protein phosphorus, lipid phosphorus, calcium and iron. All determinations were made on single eggs, and six eggs from each batch were analysed for each variable. Results were expressed in absolute terms (as weight of component per egg) and as percentage of egg dry weight. Highly significant variations in all these aspects of egg composition were shown to occur between parent females. However, there was no significant correlation between the percentage of the original number of eggs which hatched and any one aspect of egg composition, except for a weak positive correlation (P<0.05) with the percentage of protein phosphorus in the eggs. Similarly, there was no significant correlation between the percentage of the original number of eggs which reached first feeding and any one aspect of egg composition except for weak positive correlations (P<0.05) with egg wet weight and with both the absolute level and the percentage of protein phosphorus in the egg. There was significant positive correlation (P<0.05) between the percentage of hatched eggs (alevins) surviving to first feeding and each of the following: egg wet weight, egg dry weight and absolute levels in the egg of bound lipid, precipitable protein and protein phosphorus. Egg batches with higher hatching percentage (>50%) differed significantly from those with zero hatching percentage in having (in absolute terms) higher egg weight, chorion weight, protein phosphorus (all P<0.001), egg dry weight, bound lipid and precipitable protein (all P<0.01) and (percentages) higher chorion weight (P<0.05) and protein phosphorus (P<0.001), and lower free and total lipid (both P<0.01) and iron (P<0.05). In a separate experiment to investigate the effects of allowing the eggs to be retained by the female within the abdominal cavity for increasing periods of time after ovulation, eggs were obtained from three females on three or four successive occasions 2–11 days apart. Although the above aspects of egg composition remained almost constant when the eggs were held in the female for up to 18 days after ovulation, the hatching percentage declined sharply within this period, in two females falling from over 90% to near zero. These results together indicate that the time of stripping of the eggs in relation to the date of ovulation is a much more significant determinant of egg quality than any of the chemical and physical aspects of egg composition which were investigated.  相似文献   
37.
Genetically modified (GM) cassava is currently being developed to address problems of diseases that threaten the food security of farmers in developing countries. The technologies are aimed at smallholder farmers, in hopes of reducing the vulnerability of cassava production to these diseases. In this paper we examine barriers to farmers’ voice in the development of GM cassava. We also examine the role of a translational research process to enhance farmers’ voice, to understand the sources of vulnerability farmers in a group in Kenya’s Coast face, and to determine if their concerns are consistent with those of the scientists in agriculture addressing farmers’ needs. A two-way communication participatory process provided insights into the complex vulnerability context of farmers, their primary concerns with processing and markets of cassava in order to improve livelihoods, the lack of networks with two way communication flows, and the lack of information on GM technologies. The translational research engaged farmers and scientists in an iterative process where scientists are learning what farmers need, and farmers are learning about the potential benefits and risks from GM technologies, while at the same time expressing their concerns.  相似文献   
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39.
In the spring of 2013, project leaders who received funding from the John Templeton Foundation’s program “Can GM Crops Help to Feed the World?” met in England to discuss progress on funded projects and to identify common objectives and research interests. The collection of essays in this special symposium is one outcome of that meeting. This introduction provides background on the symposium’s theme of understanding the challenges to smallholder farmers having a voice. Farmer voice is important not only in debates about genetically modified crops but also for policies, technologies and other efforts designed by interests seeking ostensibly to improve the livelihoods of smallholder farmers.  相似文献   
40.
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