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1.
Gideon N. Gathaara Edward L. Glumac Peter Felker 《Forest Ecology and Management》1991,40(3-4):189-198
The productivity of Leucaena leucocephala and a superior L. pulverulenta family were examined over a 3-year period at spacings (1.5 m×1.5 m and 1.5×3.0 m) chosen to compare two mechanical weeding operations. Leucaena pulverulenta was examined since faster-growing families of this cold-hardy, low-mimosine leucaena species were recently identified. There was no significant difference in yields between the species (P = 0.9060) but there was a significant difference between spacings (P = 0.0491). There was no significant spacing×species, species×year or spacing×year interaction. The annual growth rates for the first, second, and third seasons' growth for L. leucocephala were 3.73, 10.11 and 6.00 t ha−1 at the close spacing and 3.03, 7.14 and 5.04 for the wider spacing. The annual growth-rates for the first, second and third seasons' growth for L. pulverulenta were 2.03, 8.35 and 8.31 t ha−1 at the close spacing and 1.25, 9.38 and 5.29 at the wider spacing. Despite the fact that L. leucocephala froze to the ground in the first and second winter when L. pulverulenta was nearly undamaged, the L. leucocephala coppice resprout was greater than the second year's growth for L. pulverulenta. 相似文献
2.
Mira Fine Edna Sakal Dorit Vashdi Nava Chapnik-Cohen Violet Daniel Avigdor Levanon Orli Lipshitz Arieh Gertler 《Fish physiology and biochemistry》1993,11(1-6):353-361
Carp growth hormone (cGH) cDNA, in which Cys-123 was mutated to Ala, was prepared, transferred to the expression vector, expressed
in Escherichia coli and the mutant was purified to homogeneity. The mutation only slightly improved yield of the monomeric fraction, indicating
that Cys-123 is not involved in improper refolding. As compared to cGH, the mutant (cGH-C123A) exhibited lower binding affinity
toward homologous liver receptors and lower bioactivity in a 3T3-F442A preadipocyte bioassay despite the fact that both hormones
exhibited almost identical cross-reactivity with anti-cGH antibodies. These results, along with those of a structural comparison
to hGH, suggest that Cys-123 is located in the hydrophobic core of the hormone, and is most likely affecting the conformation
of the binding site. Dimeric forms of the hormone and its mutant were less active than their respective monomers. Homologous
binding experiments using a carp liver microsomal fraction revealed a single receptor population with Kd = 0.77 nM and Bmax = 241 fmol/mg microsomal protein.
Résumé Un ADN complémentaire (cDNA) de l'hormone de croissance de carpe (cGH), dans lequel l'acide aminé Cys-123 a été muté en Ala, a été préparé, inséré dans un vecteur d'expression, et exprimé dans Escherichia coli. Le mutant a ensuite été purifié jusqu'à homogénéité. La mutation améliore seulement faiblement la production de la fraction monomérique, indiquant que le Cys-123 n'est pas impliqué dans un repliement erroné. Comparé à la cGH, sa forme mutée (cGH-C123A) montre une plus faible affinité de liaison vis à vis de récepteurs hépatiques homologues, et une plus faible activité biologique dans un test réalisé sur des préadipocytes 3T3-F442A; cela en dépit du fait que les deux hormones présentent des réactions croisées presque identiques avec un anticorps anti-cGH polyclonale. Ces résultats, associés à une comparaison à la structure de l'hGH, suggèrent que le Cys-123 est localisé dans la partie hydrophobique de l'hormone, et affecte, le plus vraisemblablement, la conformation du site de liaison. Les formes dimériques de l'hormone et de sa forme mutée sont moins actives que leurs monomères respectifs. Les études de liaison homologue, réalisées avec des fractions microsomales de foie, révèlent une population unique de récepteurs de Kd = 0,77 nM et de Bmax = 241 fmol/mg de proteine microsomale.相似文献
3.
Corinne Valdivia M. Kengo Danda Dekha Sheikh Harvey S. James Jr. Violet Gathaara Grace Mbure Festus Murithi William Folk 《Agriculture and Human Values》2014,31(4):673-681
Genetically modified (GM) cassava is currently being developed to address problems of diseases that threaten the food security of farmers in developing countries. The technologies are aimed at smallholder farmers, in hopes of reducing the vulnerability of cassava production to these diseases. In this paper we examine barriers to farmers’ voice in the development of GM cassava. We also examine the role of a translational research process to enhance farmers’ voice, to understand the sources of vulnerability farmers in a group in Kenya’s Coast face, and to determine if their concerns are consistent with those of the scientists in agriculture addressing farmers’ needs. A two-way communication participatory process provided insights into the complex vulnerability context of farmers, their primary concerns with processing and markets of cassava in order to improve livelihoods, the lack of networks with two way communication flows, and the lack of information on GM technologies. The translational research engaged farmers and scientists in an iterative process where scientists are learning what farmers need, and farmers are learning about the potential benefits and risks from GM technologies, while at the same time expressing their concerns. 相似文献
4.
Miyuki Iiyama David Newman Cristel Munster Meshack Nyabenge Gudeta W. Sileshi Violet Moraa James Onchieku Jeremias Gasper Mowo Ramni Jamnadass 《Agroforestry Systems》2013,87(4):729-746
With the global bioenergy boom, the planting of jatropha (Jatropha curcas) was widely promoted by the private sector and non-government organizations as one of the candidate tree species for bioenergy in Kenya. This was motivated by the belief that it grows easily with minimal management requirements. The present study attempts to determine whether management practices by smallholder farmers, which are heterogeneous, are optimal for jatropha yields in Kenya. A survey conducted in different agro-ecological zones showed that yields are very low under Kenyan farm conditions. Regardless of the age and management condition, 41 % of the farmers obtained no seed yield, while 79 % obtained up to 0.1 kg/tree. This is dismal in comparison with the figures (up to 2.0 kg/tree) reported from elsewhere for 1–5 year old trees grown under similar conditions. Examination of farmer management practices indicated that irrigation, manuring and weeding, in order to maximize yields, could be offset by misapplication of other components especially, selection of planting materials, timing of planting and choice of intercrops during the establishment phases. This indicates that the anticipated high yields have not been achieved partly because growers are still using unimproved germplasm, management practices are sub-optimal, and the biophysical boundaries of high jatropha yield are poorly defined. Thus at the current stage, jatropha should not be grown by smallholder farmers in Kenya because of low or dismal productivity. If jatropha is to play a role in the pro-poor bioenergy development, future projects need to identify management recommendations that optimize yields. This also needs to take into consideration the preferences and constraints of farm households on labor and land allocation to other farm and livelihood activities. 相似文献
5.
6.
Kang&#;ethe Erastus Kimani Violet Grace Delia Mitoko Grace McDermott Brigid Ambia Julie Nyongesa Concepta Mbugua Gabriel Ogara William Obutu Peninnah 《Tropical animal health and production》2012,44(1):41-46
This paper describes a trans-disciplinary process of co-generating and disseminating evidence-based messages for reducing the risk from cryptosporidiosis and other zoonoses in an urban community in Nairobi. Research findings about disease prevalence, risk factors and observed risky and risk-mitigating practices were analysed by a team comprising researchers, community members and local policy- and decision-makers. Using participatory planning, multiple strategies were developed for disseminating key information. We identified five vulnerable groups at higher risk of exposure to cryptosporidiosis and other cattle zoonoses with similar transmission pathways (women, children, elderly people, immunosuppressed people and male farm workers). For each group, targeted messages were developed. Good practices already in use, as were also practices as practices to improve environmental conditions. These messages were disseminated through printed material, in a workshop, through community campaigners and also an edutainment soap opera episode broadcast on Kenyan television. In conclusion, a participatory and trans-disciplinary process can help transform the findings of research into messages that are targeted, attractive and understandable. 相似文献
7.
Nyangaga Julius N. Grace Delia Kimani Violet Kiragu Monica W. Langat Alfred K. Mbugua Gabriel Mitoko Grace Kang&#;ethe Erastus K. 《Tropical animal health and production》2012,44(1):47-51
A study was undertaken to investigate and mitigate the risk from zoonotic Cryptosporidium associated with dairy farming in Dagoretti division, Nairobi, Kenya. Outcome mapping (OM), a relatively new tool for planning and evaluation, was used to foster and then monitor changes in farmer management of health risks. Elements of the OM framework, including the vision, mission and expected progress markers, were developed in participatory sessions and a set of progress markers was used for monitoring behaviour change in farmers participating in the project (the boundary partners). Behaviour change (the outcome challenge) was supported by a range of awareness and educational campaigns, working with strategic partners (extension agents and administrative leaders). The farmers the project worked with made considerable progress according to the markers; they demonstrated an understanding of cryptosporidiosis, established or maintained clean and well drained cattle sheds, and took conscious effort to reduce possible infection. Farmers who did not participate in the project (non-contact farmers) were found to be less advanced on the progress marker indicators. Non-contact farmers who carried out risk-reducing practices had done so independently of the project team. The administration leaders, as strategic partners, had a positive attitude towards the project and confidence in their ability to support project objectives. The study demonstrates the utility of OM in helping to identify and support behavioural change. 相似文献
8.
JN Nyangaga D Grace V Kimani MW Kiragu AK Langat G Mbugua G Mitoko EK Kang'ethe 《Tropical animal health and production》2012,44(Z1):47-51
A study was undertaken to investigate and mitigate the risk from zoonotic Cryptosporidium associated with dairy farming in Dagoretti division, Nairobi, Kenya. Outcome mapping (OM), a relatively new tool for planning and evaluation, was used to foster and then monitor changes in farmer management of health risks. Elements of the OM framework, including the vision, mission and expected progress markers, were developed in participatory sessions and a set of progress markers was used for monitoring behaviour change in farmers participating in the project (the boundary partners). Behaviour change (the outcome challenge) was supported by a range of awareness and educational campaigns, working with strategic partners (extension agents and administrative leaders). The farmers the project worked with made considerable progress according to the markers; they demonstrated an understanding of cryptosporidiosis, established or maintained clean and well drained cattle sheds, and took conscious effort to reduce possible infection. Farmers who did not participate in the project (non-contact farmers) were found to be less advanced on the progress marker indicators. Non-contact farmers who carried out risk-reducing practices had done so independently of the project team. The administration leaders, as strategic partners, had a positive attitude towards the project and confidence in their ability to support project objectives. The study demonstrates the utility of OM in helping to identify and support behavioural change. 相似文献
9.
Development and delivery of evidence-based messages to reduce the risk of zoonoses in Nairobi, Kenya
E Kang'ethe V Kimani D Grace G Mitoko B McDermott J Ambia C Nyongesa G Mbugua W Ogara P Obutu 《Tropical animal health and production》2012,44(Z1):41-46
This paper describes a trans-disciplinary process of co-generating and disseminating evidence-based messages for reducing the risk from cryptosporidiosis and other zoonoses in an urban community in Nairobi. Research findings about disease prevalence, risk factors and observed risky and risk-mitigating practices were analysed by a team comprising researchers, community members and local policy- and decision-makers. Using participatory planning, multiple strategies were developed for disseminating key information. We identified five vulnerable groups at higher risk of exposure to cryptosporidiosis and other cattle zoonoses with similar transmission pathways (women, children, elderly people, immunosuppressed people and male farm workers). For each group, targeted messages were developed. Good practices already in use, as were also practices as practices to improve environmental conditions. These messages were disseminated through printed material, in a workshop, through community campaigners and also an edutainment soap opera episode broadcast on Kenyan television. In conclusion, a participatory and trans-disciplinary process can help transform the findings of research into messages that are targeted, attractive and understandable. 相似文献
10.
Kang&#;ethe Erastus K. Kimani Violet N. McDermott Brigid Grace Delia Lang&#;at Alfred K. Kiragu Monica W. Karanja Nancy Njehu Alice N. Randolph Thomas Mbugua Gabriel Irungu Tabitha W. Ombutu Peninnah 《Tropical animal health and production》2012,44(1):3-10
This paper characterises the dairy farming system in Dagoretti, Nairobi. Characterisation was part of a broader ecohealth project to estimate the prevalence and risk of cryptosporidiosis and develop risk mitigation strategies. In the project a trans-disciplinary team addressed epidemiological, socioeconomic, environmental and policy aspects of cryptosporidiosis, an emerging zoonosis. This paper also provides background and describes sampling methods for the wider project. Three hundred dairy households were probabilistically sampled from a sampling frame of all dairy households in five of the six locations of Dagoretti, one of the eight districts of Nairobi Province. Randomly selected households identified 100 non-dairy-keeping households who also took part in the study. A household questionnaire was developed, pre-tested and administered in the dry and wet seasons of 2006. An additional study on livelihood and economic benefits of dairying took place with 100 dairy farmers randomly selected from the 300 farms (as well as 40 non-dairy neighbours as a control group), and a risk-targeted survey of environmental contamination with Cryptosporidium was conducted with 20 farmers randomly selected from the 29 farmers in the wider survey who were considered at high risk because of farming system. We found that around 1 in 80 urban households kept dairy cattle with an average of three cattle per household. Cross-breeds of exotic and local cattle predominate. Heads of dairy-keeping households were significantly less educated than the heads of non-dairy neighbours, had lived in Dagoretti for significantly longer and had significantly larger households. There was a high turnover of 10 % of the cattle population in the 3-month period of the study. Cattle were zero grazed, but productivity parameters were sub-optimal as were hygiene and husbandry practices. In conclusion, dairy keeping is a minor activity in urban Nairobi but important to households involved and their community. Ecohealth approaches are well suited to tackling the complex problem of assessing and managing emerging zoonoses in urban settings. 相似文献