Preparation and characterization of anti-hBLyS monoclonal antibody by DNA immunization     

ZHANG Zhi-fang  ZHANG Chun-yan  PAN Jing-yun  LIN Xue-yan 《园艺学报》2002,18(6):625-629

AIM:To establish the monoclonal antibody against human B lymphocyte stimulator (hBLyS) by DNA immunization and analyse its characterization. METHODS:The 858 bp DNA fragment of hBLyS was cloned into pcDNA3 plasmids. The cloned insert was identified by both sequence analysis and double digestion of the recombinant plasmid with restriction enzymesXho Iand EcoR I. After the splenocytes from BALB/c mice immunized with the recombinant plasmid of pcDNA3/hBLyS were fused with myeloma cells SP2/0,the hybridoma which can produce monoclonal antibodies against hBLyS were obtained. The specificity of anti-BLyS monoclonal antibody from hybridoma was verified by ELISA, Western blot and flow cytometry. RESULTS:The recombinant mammalian cell expression vector of pcDNA3/hBLyS was constructed,the sequence of the insert gene was identified to be the sequence encoding hBLy S antigen. The culture supernatants of hybridoma 9c10 were tested to be the monoclonal antibody with specificity against hBLyS on human peripheral blood CD3⁺T cell activated by hIFN-γ by ELISA,Western blot and flow cytometry.CONCLUSION:The monoclonal antibodies against hBLyS with high activity and specificity have been established successfully, and will be an useful tool in the studies of relationship between hBLyS and human autoimmunity diseases.  相似文献   

The genetic susceptibility of HLA-DRB1 alleles in esophageal neoplasm of Hubei Han Chinese     

LIN Jun  SUN Jie  ZHOU Yan  HUANG Xing  XIONG Ping  WANG Ya-ping  DENG Chang-sheng 《园艺学报》2002,18(9):1112-114

AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls(0.2500 vs 0.1397, P =0.028; the odds ratiO².053; etiologic fraction 0.1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exoN²)published in GenBank.  相似文献   

大果型鲜食枣新品种‘大白铃’   总被引：1，自引：0，他引：1  

高丽  郭裕新  周广芳  单公华  王斌 《园艺学报》2002,29(1):85-85

 ‘大白铃’为大果型鲜食枣优良新品种。果实特大, 平均单果质量24. 5 ~ 25. 9 g, 最大80 g,整齐, 外形美观, 品质优良。早实, 丰产, 抗逆性强。  相似文献   

枯草芽孢杆菌（Bacillus subtilis）S9对植物病原真菌的溶菌作用   总被引：19，自引：1，他引：19  

呈福  李德葆 《植物病理学报》2003,33(2):174-177

本研究共分离了976株细菌分离物，发现来自甘蔗根围的1株枯草芽孢杆菌(Bacillus subtilis)S9对立枯丝核菌(Rhizoctonia solani)、终极腐霉(Pythium ultimum)和西瓜枯萎病菌(Fusarium oxysporum f.sp.niveum)在PDA平板的对峙培养过程中不形成抑菌圈，但4d后可使上述植物病原真菌的菌丝溶解。扫描电镜观察发现S9菌株在待测的立枯丝核菌表面形成了溶菌斑。S9菌株对立枯丝核菌的作用过程是通过吸附在病原真菌的菌丝上，并随菌丝生长而生长，而后产生溶菌物质消解菌丝体。液体共培养测定也证明了S9菌株具有上述作用。本研究还发现，S9菌株对植物病原真菌的拮抗真菌绿色木霉(Trichoderma viride)、鲜红毛壳菌(Chaetomium cupreum)和球毛壳菌（Chaetomium globosum）的生长没有影响。盆栽试验证明了S9菌株可有效地控制立枯丝核菌(R．solani)引起的番茄苗期病害。S9菌株与其它拮抗真菌混合具有促进防治植物病原真菌引起的植物病害的潜力。  相似文献   

山羊传染性胸膜肺炎支原体和绵羊肺炎支原体对抗菌药物敏感性的研究   总被引：4，自引：0，他引：4  

林密  柴静  孙继国  王爱华 《中国兽药杂志》2004,38(5):29-32

测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰乐菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y-98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰乐菌素对Y-goat和四环素与氧氟沙星、泰乐菌素对Y-98的联合药敏作用.结果表明,这8种抗菌药物对Y-goat和Y-98的MIC(μg/mL)分别为:环丙沙星0.223、0.002 23,氧氟沙星0.281、0.014 0,单诺沙星0.136、0.014 0,红霉素0.021 8、无效,罗红霉素0.032 7、无效,泰乐菌素0.042 2、0.039 0,泰妙菌素0.021 7、0.052 0,四环素0.195、0.052 0.红霉素与氧氟沙星的联合药敏指数为1,是相加作用;红霉素与泰乐菌素对Y-goat的联合药敏指数为1.5,是无关作用;四环素与氧氟沙星、泰乐菌素对Y-98的联合药敏试验指数均为0.375,是协同作用.  相似文献   

不同培养基对杏鲍菇菌丝生长的影响   总被引：7，自引：0，他引：7  

刘宇  陈文良  林秀敏  王丽珍  孟莉莉 《中国食用菌》2004,23(5):22-24

比较了杏鲍菇一级种和二级种培养基配方 ,试验表明杏鲍菇一级种培养基以配方三最好 ,菌丝干重是配方七 (对照 )的 3 5倍 ,差异极显著 ;杏鲍菇二级种培养基以配方四最好 ,菌丝生长势最强 ,与供试各培养基配方相比较 ,差异均为极显著  相似文献   

干旱胁迫下保水剂对苗木生长及生理的影响   总被引：10，自引：0，他引：10  

林文杰  马焕成  周蛟  张志斌  甘云浩 《干旱区研究》2004,21(4):353-357

在盆栽条件下,对不同保水剂处理的土壤吸持水特性以及云南拟单性木兰和绒叶含笑苗的水分生理、生长状况进行试验研究。结果表明,随着保水剂用量的增加,土壤保水能力和供水能力增加,苗木在有限水分条件下生存期延长3-91 d,地径、树高、新增叶片分别增加了16.7％-461.2％,22.7％-368.2％,42.9％-372.7％;随着保水剂用量增加,土壤容重下降了6.2％-35.1％,总孔隙度和毛管孔隙度明显增加了3.6％-20.3％和5.9％-22.6％;在有限水分条件下,通过不同保水剂处理能减少水分的胁迫,净光合速率增长8.9％-461.2％,蒸腾速率增长了22.2％-518.7％,气孔导度增长了4.2％-512.5％。  相似文献   

检疫害虫稻水象甲的控制技术研究进展   总被引：5，自引：0，他引：5  

黄澍  胡美英官珊 《昆虫天敌》2004,26(2):86-91

稻水象甲(Lissorhoptrus。oryzophilus Kuschel)是一种毁灭性的水稻害虫，也是一种重要的检疫害虫。近年来在很多省市发生为害，且发生程度不断加重并呈扩大蔓延趋势。本文概述了近年来稻水象甲大发生危害的情况及原因，深入分析了其发生规律，提出了加强植物检疫、农业防治、化学防治、生物防治、物理防治等综合治理的措施。  相似文献   

The molecular mechanism of inhibition of murine Lewis lung carcinoma metastasis by weimaining in vivo     

LOU Jin-li  LIN Hong-sheng  QIU Quan-ying  PEI Ying-xia  QI Xin  HE Xiu-juan 《园艺学报》2004,20(4):627-630

AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg·kg^-1·d^-1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue.  相似文献   

亚洲璃眼蜱唾液腺一新功能基因的克隆与测序   总被引：1，自引：0，他引：1  

程天印  周金林  施启顺  周勇志  林矫矫 《畜牧与兽医》2005,37(5):4-6

HaB1是亚洲璃眼蜱雌成蜱唾液腺差异表达基因文库中的一个片段,根据其序列设计引物HaB1-GSP1和HaB1-GSP2,以唾液腺总RNA为模板,RACE法扩增获得HaB1的未知3′-末端。测定该末端序列,进行序列拼接,设计全长引物5-′CCAGTCCGAAGGAAGGGCG-3′和5-′TCTC-CGGGCACGTGAAGTGTC-3′。以cDNA第一链为模板,扩增获得基因全长。经核查表明,该基因为一新基因(登录号AY803896)。  相似文献